Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable translation initiation factor eIF-2B subunit beta

Gene

tif222

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor eIF-2B subunit beta
Alternative name(s):
eIF-2B GDP-GTP exchange factor subunit beta
Gene namesi
Name:tif222
ORF Names:SPAC343.14c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC343.14c.
PomBaseiSPAC343.14c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 2B complex Source: PomBase
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Probable translation initiation factor eIF-2B subunit betaPRO_0000317323Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei106 – 1061Phosphoserine1 Publication
Modified residuei108 – 1081Phosphoserine1 Publication
Modified residuei112 – 1121Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UT76.

PTM databases

iPTMnetiQ9UT76.

Interactioni

Subunit structurei

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.By similarity

Protein-protein interaction databases

BioGridi279470. 7 interactions.
MINTiMINT-4709788.

Structurei

Secondary structure

1
393
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 74Combined sources
Helixi11 – 2111Combined sources
Helixi28 – 4518Combined sources
Helixi51 – 6818Combined sources
Helixi73 – 9624Combined sources
Helixi141 – 1444Combined sources
Helixi170 – 18415Combined sources
Helixi187 – 1915Combined sources
Helixi192 – 1976Combined sources
Beta strandi204 – 2096Combined sources
Helixi212 – 22211Combined sources
Beta strandi228 – 2325Combined sources
Turni235 – 2373Combined sources
Helixi239 – 25113Combined sources
Beta strandi255 – 2595Combined sources
Helixi261 – 2633Combined sources
Helixi264 – 2674Combined sources
Helixi268 – 2703Combined sources
Beta strandi273 – 2764Combined sources
Beta strandi279 – 2813Combined sources
Beta strandi287 – 2904Combined sources
Helixi293 – 30210Combined sources
Beta strandi307 – 3104Combined sources
Helixi313 – 3153Combined sources
Helixi324 – 3274Combined sources
Helixi333 – 3353Combined sources
Helixi342 – 3465Combined sources
Beta strandi347 – 3504Combined sources
Beta strandi355 – 3584Combined sources
Helixi360 – 3623Combined sources
Beta strandi365 – 3684Combined sources
Beta strandi371 – 3733Combined sources
Helixi375 – 3773Combined sources
Helixi378 – 3858Combined sources
Helixi388 – 3914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99C/D1-393[»]
ProteinModelPortaliQ9UT76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000208487.
InParanoidiQ9UT76.
KOiK03754.
OMAiFVSPHEV.
OrthoDBiEOG70KH0H.
PhylomeDBiQ9UT76.

Family and domain databases

InterProiIPR000649. IF-2B-related.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UT76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTINVEHTY PAVSSLIADL KSRKVQGPFA VAVETALVMR QVISQTRWST
60 70 80 90 100
VDQLIDTVRA VGSTLVKAQP TEFSCGNIIR RILRLIREEY QELLKTADEN
110 120 130 140 150
EKLIVSSSNS SSPSQKRDIP SNEKLVQSHE PVSVQMYSSM LNLLGRPTLE
160 170 180 190 200
SPTHSKTVGD SRVTGGMDMR AVIISGIQDV IDELDKINTD IEVQSMDHLH
210 220 230 240 250
SNEIILTQGC SKTVEAFLRF AAKKRKFSVI VAEGFPNNQK GSHAMAKRLA
260 270 280 290 300
QAGIDTTVIS DATIFAIMSR VNKVILGTHA ILGNGGLVTY SGAQLVAQAA
310 320 330 340 350
RHHATPVVVC SGIYKLSPVY PYDLESIIQL SSPDKIMSFN EGDLISRAEI
360 370 380 390
LNPYYDYIPP DLVDLFITNL GGYPPSYLYR IMNDTYDASD TIL
Length:393
Mass (Da):43,221
Last modified:May 1, 2000 - v1
Checksum:i19F324EF0135628B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB52277.1.
PIRiT38663.
RefSeqiNP_593435.1. NM_001018868.2.

Genome annotation databases

EnsemblFungiiSPAC343.14c.1; SPAC343.14c.1:pep; SPAC343.14c.
GeneIDi2543035.
KEGGispo:SPAC343.14c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB52277.1.
PIRiT38663.
RefSeqiNP_593435.1. NM_001018868.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99C/D1-393[»]
ProteinModelPortaliQ9UT76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279470. 7 interactions.
MINTiMINT-4709788.

PTM databases

iPTMnetiQ9UT76.

Proteomic databases

MaxQBiQ9UT76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC343.14c.1; SPAC343.14c.1:pep; SPAC343.14c.
GeneIDi2543035.
KEGGispo:SPAC343.14c.

Organism-specific databases

EuPathDBiFungiDB:SPAC343.14c.
PomBaseiSPAC343.14c.

Phylogenomic databases

HOGENOMiHOG000208487.
InParanoidiQ9UT76.
KOiK03754.
OMAiFVSPHEV.
OrthoDBiEOG70KH0H.
PhylomeDBiQ9UT76.

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiQ9UT76.

Family and domain databases

InterProiIPR000649. IF-2B-related.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; SER-108 AND SER-112, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEI2BB_SCHPO
AccessioniPrimary (citable) accession number: Q9UT76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.