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Q9UT61

- MAN1_SCHPO

UniProt

Q9UT61 - MAN1_SCHPO

Protein

Alpha-mannosidase

Gene

ams1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Degrades free oligosaccharides in the vacuole.By similarity

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi290 – 2901ZincBy similarity
    Metal bindingi292 – 2921ZincBy similarity
    Active sitei402 – 4021NucleophileBy similarity
    Metal bindingi402 – 4021ZincBy similarity
    Metal bindingi615 – 6151ZincBy similarity

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: PomBase
    2. carbohydrate binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mannose metabolic process Source: InterPro
    2. oligosaccharide catabolic process Source: PomBase

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-mannosidase (EC:3.2.1.24)
    Alternative name(s):
    Alpha-D-mannoside mannohydrolase
    Gene namesi
    Name:ams1
    Synonyms:mns2
    ORF Names:SPAC513.05
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC513.05.

    Subcellular locationi

    Cytoplasm 1 Publication. Vacuole By similarity

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. fungal-type vacuole membrane Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10771077Alpha-mannosidasePRO_0000363389Add
    BLAST

    Proteomic databases

    MaxQBiQ9UT61.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4709700.
    STRINGi4896.SPAC513.05-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UT61.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Phylogenomic databases

    eggNOGiCOG0383.
    HOGENOMiHOG000048425.
    KOiK01191.
    OMAiCHMAPSE.
    OrthoDBiEOG79SF5G.
    PhylomeDBiQ9UT61.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9UT61-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLFPVLNNT PVGKQVDSIY ESRLDQFLSE GQYRDFNLPS VYDHARIDNP     50
    SGDVNNDLSK GFVDLKVYRV PDLSRPSFNE VVGHKKFDET ASKGDTFGPS 100
    WATFWFEVHI RLPKSWAKYE QVIFQWNCDN EGLVYSQDGV PLQAFSGSER 150
    TDFILPDSWK TTEDTFYIEM ACNGMFGTGA GSQIAPPDPN RYFTLTKADL 200
    VAPNLPAMAL AYDFLLMQQC VKQLPSNCWQ KYKARQICND IMNTFHPNDL 250
    STINECRNLA KAFLGNDIDS EAVFEKNNDK ANVFAIGHCH IDTAWLWPFA 300
    ETRRKIVRSW ATQMNIMDRY PEYQFVCSQA LQYLWLKEDH PDVFEKLKEY 350
    VNQNKFIPIG GSWVEHDTNI PNGESLIRQF LLGQHFFEKE FGVRCRTFWL 400
    PDTFGYSSQI PQICRLCGMD RFLTQKLSWN NINSFPTSTF NWVALDGSQV 450
    ICHMPPANTY TADTNVNDVL HSIDQHKNLV NDQAGLLVFG IGDGGGGPTP 500
    EMLEKLRRCK GIANTVGYLP NVKLGNTVDE FFDGILKRTN AGQTLPSWNG 550
    ELYFEFHRGT YTTQAELKKL MRKVEIALHD AEYVSTLASI FSKDYSYPKE 600
    SLQDLWRDTL LCQFHDVLPG SCIEMVYKDA IPIMSKVLKN TEALLWQAIE 650
    QLGFKKASSS DNKEQLCLLN TLPWNVRGVI TETEENKLVY FESCDGKGIL 700
    TAAHTSLKHP AAAYQKDDNF ILVNDHLRVT IAPNGLILSL FDLHKEREIL 750
    DLKSGKNHAG ANQYVLFEDT PLSWQAWDTE VFSLEKYEVL DKGKVSIKES 800
    GPLRASVVVD IPISELSHMK ATISLEGYND CSEFTGVNFT CEVDWHESCK 850
    FLKVEFPVDI HSEFASYETQ FGITKRPTHY NTSWDVAKFE VCHQKFADYS 900
    DFTYGVSVLN DCKYGFSTHG NLMRLSLLRS PKQPDAHADM GKHTIRYAVY 950
    PHSKPLDSST VRAAHKFNSN FRLLTRASDT ANLDIFDAFQ LVGEPNVILS 1000
    HIKMAEKGKS IILRVYESLG GKSRARLVIK SLTVASVTKC NGLEEDLEEL 1050
    CTLKSNDYYE VPIELRAFEI ATFKVNL 1077
    Length:1,077
    Mass (Da):122,438
    Last modified:May 1, 2000 - v1
    Checksum:i8774DDF4A2194A46
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB58728.1.
    PIRiT38900.
    RefSeqiNP_593979.1. NM_001019405.2.

    Genome annotation databases

    EnsemblFungiiSPAC513.05.1; SPAC513.05.1:pep; SPAC513.05.
    GeneIDi2543485.
    KEGGispo:SPAC513.05.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB58728.1 .
    PIRi T38900.
    RefSeqi NP_593979.1. NM_001019405.2.

    3D structure databases

    ProteinModelPortali Q9UT61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4709700.
    STRINGi 4896.SPAC513.05-1.

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    Proteomic databases

    MaxQBi Q9UT61.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC513.05.1 ; SPAC513.05.1:pep ; SPAC513.05 .
    GeneIDi 2543485.
    KEGGi spo:SPAC513.05.

    Organism-specific databases

    PomBasei SPAC513.05.

    Phylogenomic databases

    eggNOGi COG0383.
    HOGENOMi HOG000048425.
    KOi K01191.
    OMAi CHMAPSE.
    OrthoDBi EOG79SF5G.
    PhylomeDBi Q9UT61.

    Miscellaneous databases

    NextBioi 20804497.
    PROi Q9UT61.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMAN1_SCHPO
    AccessioniPrimary (citable) accession number: Q9UT61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3