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Protein

Probable cytosolic Fe-S cluster assembly factor SPAC806.02c

Gene

SPAC806.02c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Fusion protein of two essential components of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. May form a heterotetramer with nubp35, functioning as a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (By similarity).By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of nbp35 and two labile, bridging clusters between subunits of the nbp35-SPAC806.02c heterotetramer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi201Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi204Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 20ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosolic Fe-S cluster assembly factor SPAC806.02c
Gene namesi
ORF Names:SPAC806.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC806.02c.
PomBaseiSPAC806.02c.

Subcellular locationi

GO - Cellular componenti

  • CIA complex Source: InterPro
  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002788831 – 608Probable cytosolic Fe-S cluster assembly factor SPAC806.02cAdd BLAST608

Proteomic databases

MaxQBiQ9UT57.
PRIDEiQ9UT57.

Interactioni

Subunit structurei

Heterotetramer of 2 nbp35 and 2 SPAC806.02c chains.By similarity

Protein-protein interaction databases

MINTiMINT-4709672.

Structurei

3D structure databases

ProteinModelPortaliQ9UT57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati288 – 327WD 1Add BLAST40
Repeati331 – 371WD 2Add BLAST41
Repeati376 – 415WD 3Add BLAST40
Repeati421 – 460WD 4Add BLAST40
Repeati465 – 504WD 5Add BLAST40
Repeati529 – 567WD 6Add BLAST39
Repeati576 – 608WD 7Add BLAST33

Sequence similaritiesi

In the N-terminal section; belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.Curated
In the C-terminal section; belongs to the WD repeat CIA1 family.Curated
Contains 7 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

InParanoidiQ9UT57.
OrthoDBiEOG092C4BLN.
PhylomeDBiQ9UT57.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
Gene3Di2.130.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03037. ciao1. 1 hit.
MF_02040. Mrp_NBP35. 1 hit.
MF_03039. NUBP2. 1 hit.
InterProiIPR028608. CIAO1/Cia1.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR033756. YlxH/NBP35.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UT57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVQHVILV LSGKGGVGKS SVTTQLALSL HDSKVYSRPL KTGILDIDLT
60 70 80 90 100
GPSIPRMFGK DAERNRIHQS SAGWVPVYTD ETKEIGLMSL GFLLTSKNDS
110 120 130 140 150
VVWRGPKKAA MIRQFISDVS WGELDFLIID TPPGTGDEHL TIVESLLSET
160 170 180 190 200
STVRDVPIDG AVIVTTPQGI ATLDVQKEID FCKKASIKIL GIVENMSGYI
210 220 230 240 250
CPHCADCTNI FSSGGGLTLS EKYKLPFLGS VPIDPKFGEM IENLTPDSNI
260 270 280 290 300
VHLYSKTEMS KKFSFITNEF LNQLYGPRKL DTITTISGHT GRLWSVAAHP
310 320 330 340 350
MLPLFATSSQ DKSVRIYNSN TYNLVHVIDG FHTRSIRRVA WRPIERPVLA
360 370 380 390 400
VASFDSVVSI NEKIDDDWEC TAALEGHENE VKCIAWSCNG NYLATCSRDK
410 420 430 440 450
SVWIWEATED DEFDCLAVLQ EHTQDVKVVT WHPTEDLLVS GSYDNSICFW
460 470 480 490 500
RDDGDDWALT CQLQGHTNTV WALAFSPNGN TLASADNDGN VFLWIKISSN
510 520 530 540 550
EDVATIDSTN ILRPALQEEW KQQTSLPHIH KGAVYTISWM NDATLCSAGG
560 570 580 590 600
DGKIVVYQRE KHDEALWHVA YEQDHAHGVY EINSLEYLRD DRLLSGGDDG

ECRVWSFK
Length:608
Mass (Da):67,680
Last modified:May 1, 2000 - v1
Checksum:i151FD4B532E81357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB55281.1.
PIRiT39094.
RefSeqiNP_592852.1. NM_001018253.2.

Genome annotation databases

EnsemblFungiiSPAC806.02c.1; SPAC806.02c.1:pep; SPAC806.02c.
GeneIDi2542122.
KEGGispo:SPAC806.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB55281.1.
PIRiT39094.
RefSeqiNP_592852.1. NM_001018253.2.

3D structure databases

ProteinModelPortaliQ9UT57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4709672.

Proteomic databases

MaxQBiQ9UT57.
PRIDEiQ9UT57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC806.02c.1; SPAC806.02c.1:pep; SPAC806.02c.
GeneIDi2542122.
KEGGispo:SPAC806.02c.

Organism-specific databases

EuPathDBiFungiDB:SPAC806.02c.
PomBaseiSPAC806.02c.

Phylogenomic databases

InParanoidiQ9UT57.
OrthoDBiEOG092C4BLN.
PhylomeDBiQ9UT57.

Miscellaneous databases

PROiQ9UT57.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
Gene3Di2.130.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03037. ciao1. 1 hit.
MF_02040. Mrp_NBP35. 1 hit.
MF_03039. NUBP2. 1 hit.
InterProiIPR028608. CIAO1/Cia1.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR033756. YlxH/NBP35.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCFD1_SCHPO
AccessioniPrimary (citable) accession number: Q9UT57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Results from a fusion of two genes coding for two proteins which both play a role in assembly of Fe-S clusters in other species (CFD1 and CIA1 in Saccharomyces cerevisiae).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.