Q9UT49 (CID13_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) RNA polymerase cid13
Short name=PAP
EC=2.7.7.19

Alternative name(s):
Caffeine-induced death protein 13
Polynucleotide adenylyltransferase cid13

Gene names

Name:	cid13
ORF Names:	SPAC821.04c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Polymerase that creates the 3' poly(A) tail of suc22 mRNA. Ref.2
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Interacts with pab1. Ref.2
Subcellular location	Cytoplasm. Nucleus Ref.2 Ref.3.
Sequence similarities	Belongs to the DNA polymerase type-B-like family. Contains 1 PAP-associated domain.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from direct assay Ref.2. Source: PomBase mitotic DNA replication checkpoint Inferred from mutant phenotype PubMed 12218190. Source: PomBase
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: PomBase nucleus Inferred from direct assay Ref.3. Source: PomBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA 3'-UTR binding Inferred by curator. Source: PomBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from direct assay Ref.2. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Poly(A) RNA polymerase cid13		PRO_0000120313
Regions
	Domain	275 – 330	56	PAP-associated
	Compositional bias	551 – 556	6	Poly-Ser
Sites
	Metal binding	110	1	Magnesium or manganese; catalytic By similarity
	Metal binding	112	1	Magnesium or manganese; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UT49 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A572D21E13552351	FASTA	578	65,771
	10 20 30 40 50 60 MDNANCVGGC KFETRSFQYR RRIPYSLGAD PLPPVHPLSL KNLVDIDTDL ISSQLYELYD 70 80 90 100 110 120 SIILNDSGLE RRYAFVQKLE QILKKEFPYK NIKTSLFGST QSLLASNASD IDLCIITDPP 130 140 150 160 170 180 QCAPTTCEVS AAFARNGLKK VVCISTAKVP IVKVWDSELQ LSCDCNINKT ISTLNTRLMR 190 200 210 220 230 240 SYVLCDPRVR PLIVMIKYWA KRRCLNDAAE GGTLTSYTIS CMVINFLQKR DPPILPSLQM 250 260 270 280 290 300 LPHLQDSSTM TDGLDVSFFD DPDLVHGFGD KNEESLGILF VEFFRFFGYL FDYEHFVLSI 310 320 330 340 350 360 RHGTFLSKRA KGWQFQLNNF LCVEEPFHTS RNLANTADEI TMKGIQLEFR RVFRLLAYNC 370 380 390 400 410 420 NVDDACSQFT FPSLTDTSFM DDYVNELQLE IVPGFSHGRD SSDTSCTESP PEPSHFAWAF 430 440 450 460 470 480 DPYNATASPY YNQNINSSID YSSIYSNDVP AIPPNVPYTF VDPYTYACYI NNNSYLPPSY 490 500 510 520 530 540 MDFYTWYNSP YPKSSHHFDE RHGGDRHEKN LSNSRRYSRN KFHKKKQSSG PFQYYPDAFS 550 560 570 FTPTDNNSPP SNSSSSEVVS PVSLHSEPVL STVQAFKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA." Saitoh S., Chabes A., McDonald W.H., Thelander L., Yates J.R. III, Russell P. Cell 109:563-573(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAB1, SUBCELLULAR LOCATION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB57438.1.
PIR	T41715.
RefSeq	NP_593157.1. NM_001018555.2.
3D structure databases
ProteinModelPortal	Q9UT49.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UT49. 6 interactions.
STRING	4896.SPAC821.04c-1.
Proteomic databases
PRIDE	Q9UT49.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC821.04c.1; SPAC821.04c.1:pep; SPAC821.04c.
GeneID	2543204.
KEGG	spo:SPAC821.04c.
Organism-specific databases
PomBase	SPAC821.04c.
Phylogenomic databases
eggNOG	COG5260.
OMA	DICIIAP.
OrthoDB	EOG46QB1M.
Family and domain databases
InterPro	IPR002934. Nucleotidyltransferase. IPR002058. PAP_assoc. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF03828. PAP_assoc. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804227.

Entry information

Entry name

CID13_SCHPO

Accession

Primary (citable) accession number: Q9UT49

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program