Q9UT45 (ENG1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-1,3(4)-beta-glucanase 1
Short name=Endo-1,3-beta-glucanase 1
Short name=Endo-1,4-beta-glucanase 1
EC=3.2.1.6

Alternative name(s):
Laminarinase-1

Gene names

Name:	eng1
ORF Names:	SPAC821.09

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1016 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has a role in cell separation where it is required for the degradation of the primary septum after completion of cytokinesis. Ref.3
Catalytic activity	Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Subcellular location	Membrane. Note: Localized in a ring-like structure around the septum. Ref.2 Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 81 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro extracellular polysaccharide metabolic process Inferred by curator. Source: GeneDB_Spombe oligosaccharide catabolic process Inferred by curator. Source: GeneDB_Spombe primary cell septum disassembly Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cell septum surface Inferred from direct assay. Source: GeneDB_Spombe cell surface Inferred from direct assay. Source: GeneDB_Spombe membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endo-1,3(4)-beta-glucanase activity Inferred from electronic annotation. Source: EC glucan endo-1,3-beta-D-glucosidase activity Inferred from direct assay. Source: GeneDB_Spombe polysaccharide binding Inferred from direct assay. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 1016	996	Endo-1,3(4)-beta-glucanase 1		PRO_0000012132
Regions
	Compositional bias	752 – 956	205	Thr-rich
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UT45 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9240CA34D35786FC	FASTA	1,016	109,743
	10 20 30 40 50 60 MSSYLRSFIF GLLTISLAQC SPILKDTKDT KFSTGSNISL KKRDTNVFDS VVDTINPASY 70 80 90 100 110 120 FGTVSHPVTP AGVSTDSLSS PIETNKFFDN NLLGSRTNFM YADPFRYWWQ SSDTMGGICI 130 140 150 160 170 180 AHTDDNQRVM DTDDTIPSYY YEPIGICSLG FGASGITSNT DPIVDEIDQM SARFTFSWDS 190 200 210 220 230 240 SSMQLTLTEG MAVTTAVYTN AIPQIFSSTL YINDFVEVPG TSAVQKYRVT MSDNHVWLIY 250 260 270 280 290 300 IYGDSLTLTE STSQMLVGSN TFNGYIQIAK IPLGDGTAEA LYDTYAGVYI TGISISGYVE 310 320 330 340 350 360 DAVGYYSFDF TTAGDTSVEP LFFLLPHQVD TAVSGTKVTS IVLASLVSGD MNAAAGNSIT 370 380 390 400 410 420 FAEAIPQDIG FLPWSPTGGQ IGYSEEALEI IAEVAGTELG EDFSANSNLN SMYYSGKVLA 430 440 450 460 470 480 KYAMLCVTIN DILGDETSSE QCIQKLEAAF ARFVDNQQIY PLTYDNTWKG VVSVAGLSGD 490 500 510 520 530 540 SLADFGNSYY NDHHFHYGYF VFTAAVIGHI DPDWINTGNN KEWVNFLVRD VANPSSNDPY 550 560 570 580 590 600 FPKHRMIDIY HGHGWASGLF ESNDGKDEES TSEDYNFFFG MKLWGQVIGD SDMEDRANII 610 620 630 640 650 660 LGIERNALNK YMLYADGNVQ PTSMQPNYVA GITFMNKITH TTYFGTNIEY IQGIHMLPIT 670 680 690 700 710 720 PISAFIRGPS FVLAEWNALL ASVIDYVDSG WRSLLYANLA IAEPEESYEY FSSSDFNTDY 730 740 750 760 770 780 LDDGASRAWY LAYAAGLWAN DAVYYPVSSS STTTTSTSTG SVTTTSTTAT ASCTLPISYT 790 800 810 820 830 840 STPTTTSISG TCNGATFDAS LYVCDGTVLC PIVNGVSYQN CNGACYNPSQ YGCDNGALGP 850 860 870 880 890 900 VQSSSTTSSI TPTPTTTSSI TPTPTTTSTT TTAQSTGMQL CGSNYYDASS YYCDNDQLCP 910 920 930 940 950 960 IIDGVDYLSC NGACYNPSQY VCSDGSLSPN TVTTTKATTT FTPTPTTTTT PTPTTTSATS 970 980 990 1000 1010 TNVIAQCGSA WYDSQSYICY GNILCPIING SPLLACGNAC YDSSIYGCSN GALVAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-168, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[3]	"The endo-beta-1,3-glucanase eng1p is required for dissolution of the primary septum during cell separation in Schizosaccharomyces pombe." Martin-Cuadrado A.B., Duenas E., Sipiczki M., de Aldana C.R.V., del Rey F. J. Cell Sci. 116:1689-1698(2003) [PubMed: 12665550] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB57443.1. AB027918 Genomic DNA. Translation: BAA87222.1. Sequence problems.
PIR	T41720.
RefSeq	NP_593162.1. NM_001018560.1.
3D structure databases
ProteinModelPortal	Q9UT45.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UT45.
Protein family/group databases
CAZy	CBM52. Carbohydrate-Binding Module Family 52. GH81. Glycoside Hydrolase Family 81.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC821.09.1; SPAC821.09.1:pep; SPAC821.09.
GeneID	2543379.
GenomeReviews	Gene locus eng1 in contig CU329670_GR.
KEGG	spo:SPAC821.09.
NMPDR	fig|4896.1.peg.3132.
Organism-specific databases
GeneDB_Spombe	SPAC821.09.
Phylogenomic databases
eggNOG	fuNOG05625.
GeneTree	EFGT00050000000117.
HOGENOM	HBG398584.
OMA	GNTLYND.
OrthoDB	EOG4MKRQH.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000864-MONOMER.
Gene expression databases
ArrayExpress	Q9UT45.
Family and domain databases
InterPro	IPR018909. CBM_septum. IPR005200. Glyco_hydro_81. [Graphical view]
Pfam	PF10645. Carb_bind. 3 hits. PF03639. Glyco_hydro_81. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENG1_SCHPO

Accession

Primary (citable) accession number: Q9UT45
Secondary accession number(s): Q9UTZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program