SPAC824.07

Functionⁱ

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.By similarity

Catalytic activityⁱ

S-(2-hydroxyacyl)glutathione + H₂O = glutathione + a 2-hydroxy carboxylate.

Cofactorⁱ

Zn²⁺By similarityNote: Binds 2 Zn²⁺ ions per subunit.By similarity

Pathwayⁱ: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Lactoylglutathione lyase (glo1)
Probable hydroxyacylglutathione hydrolase C13B11.03c (SPCC13B11.03c), Probable hydroxyacylglutathione hydrolase C824.07 (SPAC824.07)

This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	63	Zinc 1; via tele nitrogenBy similarity	1
Metal bindingⁱ	65	Zinc 1; via pros nitrogenBy similarity	1
Metal bindingⁱ	67	Zinc 2By similarity	1
Metal bindingⁱ	68	Zinc 2; via tele nitrogenBy similarity	1
Metal bindingⁱ	118	Zinc 1; via tele nitrogenBy similarity	1
Metal bindingⁱ	139	Zinc 1By similarity	1
Metal bindingⁱ	139	Zinc 2By similarity	1
Binding siteⁱ	148	SubstrateBy similarity	1
Metal bindingⁱ	178	Zinc 2; via tele nitrogenBy similarity	1

GO - Molecular functionⁱ

hydroxyacylglutathione hydrolase activity Source: PomBase
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular detoxification Source: PomBase
methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: PomBase

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-SPO-70268. Pyruvate metabolism.
UniPathwayⁱ	UPA00619; UER00676.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable hydroxyacylglutathione hydrolase C824.07 (EC:3.1.2.6) Alternative name(s): Glyoxalase II Short name: Glx II
Gene namesⁱ	ORF Names:SPAC824.07
Organismⁱ	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifierⁱ	284812 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe
Proteomesⁱ	UP000002485 Componentⁱ: Chromosome I

Organism-specific databases

EuPathDBⁱ	FungiDB:SPAC824.07.
PomBaseⁱ	SPAC824.07.

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000337689	1 – 256	Probable hydroxyacylglutathione hydrolase C824.07Add BLAST		256

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9UT36.
PaxDbⁱ	Q9UT36.
PRIDEⁱ	Q9UT36.

PTM databases

iPTMnetⁱ	Q9UT36.

iPTMnetⁱ

Q9UT36.

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	278908. 1 interactor.
STRINGⁱ	4896.SPAC824.07.1.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9UT36.
SMRⁱ	Q9UT36.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	178 – 180	Substrate bindingBy similarity		3
Regionⁱ	250 – 253	Substrate bindingBy similarity		4

Sequence similaritiesⁱ

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.Curated

Phylogenomic databases

HOGENOMⁱ	HOG000058041.
InParanoidⁱ	Q9UT36.
KEGG Orthology (KO) More...KOⁱ	K01069.
OMAⁱ	NYIWLLQ.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4YMK.
PhylomeDBⁱ	Q9UT36.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Dⁱ	3.60.15.10. 1 hit.
InterProⁱ	View protein in InterPro IPR035680. Clx_II_MBL. IPR032282. HAGH_C. IPR001279. Metallo-B-lactamas. IPR036866. RibonucZ/Hydroxyglut_hydro.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16123. HAGH_C. 1 hit. PF00753. Lactamase_B. 1 hit.
SMARTⁱ	View protein in SMART SM00849. Lactamase_B. 1 hit.
SUPFAMⁱ	SSF56281. SSF56281. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9UT36-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSFHITPIWM WKGTGNNYAY LLTCDKTKIT AIVDPAEPES VIPVIKEKTA 
        60         70         80         90        100
KKEIDLQYIL TTHHHYDHAG GNEDILSYFP SLKVYGGKNA SGVTYTPKDK 
       110        120        130        140        150
EIFKVGEVQV EALHTPCHTQ DSICYYVSSP SKRAVFTGDT LFTSGCGRFF 
       160        170        180        190        200
EGDAKQMDYA LNHVLAALPD DTVTYPGHEY TKSNAKFSST IFSTPELTKL 
       210        220        230        240        250
VDFCKNHEST TGHFTIGDEK KFNPFMCLGL ESVQKAVGSS DPITVMKKLR 

DMKNAA

Length:256

Mass (Da):28,483

Last modified:May 1, 2000 - v1

Checksum:ⁱ1DA6B3A0F8BBF0FE

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329670 Genomic DNA. Translation: CAB57337.1.
PIRⁱ	T39108.
NCBI Reference Sequences More...RefSeqⁱ	NP_593446.1. NM_001018879.2.

Genome annotation databases

EnsemblFungiⁱ	SPAC824.07.1; SPAC824.07.1:pep; SPAC824.07.
GeneIDⁱ	2542447.
KEGGⁱ	spo:SPAC824.07.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q9UT36	A0A1C1XD87 A0A0G2FRB1 M7NSV2 A0A1W5D8M7 A0A1E3QEL3 UPI000866E7B6 A0A0J8RAZ2 A0A150V280 H6BMZ3 N1PVS0 +22	Diaporthe helianthi Diaporthe ampelina Pneumocystis murina (strain B123) (Mouse pneumocystis pneumonia agent) (Pneumocystis carinii f. sp. muris) Umbilicaria pustulata Lipomyces starkeyi NRRL Y-11557 Saitoella complicata NRRL Y-17804 Coccidioides immitis RMSCC 3703 Acidomyces richmondensis BFW Exophiala dermatitidis (strain ATCC 34100 / CBS 525.76 / NIH/UT8656) (Black yeast) (Wangiella dermatitidis) Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight fungus) (Mycosphaerella pini) And more	256	UniRef50_Q9UT36	Cluster: Probable hydroxyacylglutathione hydrolase C824.07	33

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329670 Genomic DNA. Translation: CAB57337.1.
PIRⁱ	T39108.
NCBI Reference Sequences More...RefSeqⁱ	NP_593446.1. NM_001018879.2.

3D structure databases

ProteinModelPortalⁱ	Q9UT36.
SMRⁱ	Q9UT36.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	278908. 1 interactor.
STRINGⁱ	4896.SPAC824.07.1.

PTM databases

iPTMnetⁱ	Q9UT36.

iPTMnetⁱ

Q9UT36.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9UT36.
PaxDbⁱ	Q9UT36.
PRIDEⁱ	Q9UT36.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	SPAC824.07.1; SPAC824.07.1:pep; SPAC824.07.
GeneIDⁱ	2542447.
KEGGⁱ	spo:SPAC824.07.

Organism-specific databases

EuPathDBⁱ	FungiDB:SPAC824.07.
PomBaseⁱ	SPAC824.07.

Phylogenomic databases

HOGENOMⁱ	HOG000058041.
InParanoidⁱ	Q9UT36.
KEGG Orthology (KO) More...KOⁱ	K01069.
OMAⁱ	NYIWLLQ.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C4YMK.
PhylomeDBⁱ	Q9UT36.

Enzyme and pathway databases

UniPathwayⁱ	UPA00619; UER00676.
Reactomeⁱ	R-SPO-70268. Pyruvate metabolism.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9UT36.

PROⁱ

PR:Q9UT36.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Dⁱ	3.60.15.10. 1 hit.
InterProⁱ	View protein in InterPro IPR035680. Clx_II_MBL. IPR032282. HAGH_C. IPR001279. Metallo-B-lactamas. IPR036866. RibonucZ/Hydroxyglut_hydro.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF16123. HAGH_C. 1 hit. PF00753. Lactamase_B. 1 hit.
SMARTⁱ	View protein in SMART SM00849. Lactamase_B. 1 hit.
SUPFAMⁱ	SSF56281. SSF56281. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GLO21_SCHPO
Accessionⁱ	Q9UT36Primary (citable) accession number: Q9UT36
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
	Last sequence update:	May 1, 2000
	Last modified:	February 28, 2018
	This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9UT36 (GLO21_SCHPO)

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Probable hydroxyacylglutathione hydrolase C824.07

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: methylglyoxal degradation

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytosol

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: methylglyoxal degradation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ