SPAC824.07

Functionⁱ

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.By similarity

Catalytic activityⁱ

S-(2-hydroxyacyl)glutathione + H₂O = glutathione + a 2-hydroxy carboxylate.

Cofactorⁱ

Zn²⁺By similarityNote: Binds 2 Zn²⁺ ions per subunit.By similarity

Pathwayⁱ: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Lactoylglutathione lyase (glo1)
Probable hydroxyacylglutathione hydrolase C824.07 (SPAC824.07), Probable hydroxyacylglutathione hydrolase C13B11.03c (SPCC13B11.03c)

This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature key	Position(s)	Length	Description
Metal bindingⁱ	63 – 63	1	Zinc 1; via tele nitrogenBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Metal bindingⁱ	65 – 65	1	Zinc 1; via pros nitrogenBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Metal bindingⁱ	67 – 67	1	Zinc 2By similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Metal bindingⁱ	68 – 68	1	Zinc 2; via tele nitrogenBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Metal bindingⁱ	118 – 118	1	Zinc 1; via tele nitrogenBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Metal bindingⁱ	139 – 139	1	Zinc 1By similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Metal bindingⁱ	139 – 139	1	Zinc 2By similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Binding siteⁱ	148 – 148	1	SubstrateBy similarity
Metal bindingⁱ	178 – 178	1	Zinc 2; via tele nitrogenBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)

Enzyme and pathway databases

Reactomeⁱ	R-SPO-70268. Pyruvate metabolism.
UniPathwayⁱ	UPA00619; UER00676.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable hydroxyacylglutathione hydrolase C824.07 (EC:3.1.2.6) Alternative name(s): Glyoxalase II Short name: Glx II
Gene namesⁱ	ORF Names:SPAC824.07
Organismⁱ	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifierⁱ	284812 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe
Proteomesⁱ	UP000002485 Componentⁱ: Chromosome I

Organism-specific databases

EuPathDBⁱ	FungiDB:SPAC824.07.
PomBaseⁱ	SPAC824.07.

Subcellular locationⁱ

Cytoplasm
1 Publication
Manual assertion based on experiment inⁱ
- Ref.2
  "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
  Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
  Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Nucleus
1 Publication
Manual assertion based on experiment inⁱ
- Ref.2
  "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
  Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
  Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

GO - Cellular componentⁱ

cytosol
Source: PomBase
Inferred from direct assayⁱ
- 16823372
nucleus
Source: PomBase
Inferred from direct assayⁱ
- 16823372

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Chainⁱ	1 – 256	256	Probable hydroxyacylglutathione hydrolase C824.07		PRO_0000337689	Add BLAST

Proteomic databases

MaxQBⁱ	Q9UT36.

MaxQBⁱ

Q9UT36.

Interactionⁱ

Protein-protein interaction databases

MINTⁱ	MINT-4709494.

MINTⁱ

MINT-4709494.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9UT36.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Regionⁱ	178 – 180	3	Substrate bindingBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)
Regionⁱ	250 – 253	4	Substrate bindingBy similarity Manual assertion inferred from sequence similarity toⁱ UniProtKB:Q16775 (GLO2_HUMAN)

Sequence similaritiesⁱ

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.Curated

Phylogenomic databases

HOGENOMⁱ	HOG000058041.
InParanoidⁱ	Q9UT36.
KOⁱ	K01069.
OMAⁱ	HVKCLST.
OrthoDBⁱ	EOG092C4YMK.
PhylomeDBⁱ	Q9UT36.

Family and domain databases

Gene3Dⁱ	3.60.15.10. 1 hit.
InterProⁱ	IPR032282. HAGH_C. IPR001279. Metallo-B-lactamas. [Graphical view]
Pfamⁱ	PF16123. HAGH_C. 1 hit. PF00753. Lactamase_B. 1 hit. [Graphical view]
SMARTⁱ	SM00849. Lactamase_B. 1 hit. [Graphical view]
SUPFAMⁱ	SSF56281. SSF56281. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9UT36-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSFHITPIWM WKGTGNNYAY LLTCDKTKIT AIVDPAEPES VIPVIKEKTA 
        60         70         80         90        100
KKEIDLQYIL TTHHHYDHAG GNEDILSYFP SLKVYGGKNA SGVTYTPKDK 
       110        120        130        140        150
EIFKVGEVQV EALHTPCHTQ DSICYYVSSP SKRAVFTGDT LFTSGCGRFF 
       160        170        180        190        200
EGDAKQMDYA LNHVLAALPD DTVTYPGHEY TKSNAKFSST IFSTPELTKL 
       210        220        230        240        250
VDFCKNHEST TGHFTIGDEK KFNPFMCLGL ESVQKAVGSS DPITVMKKLR 

DMKNAA

Length:256

Mass (Da):28,483

Last modified:May 1, 2000 - v1

Checksum:ⁱ1DA6B3A0F8BBF0FE

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329670 Genomic DNA. Translation: CAB57337.1.
PIRⁱ	T39108.
RefSeqⁱ	NP_593446.1. NM_001018879.2.

Genome annotation databases

EnsemblFungiⁱ	SPAC824.07.1; SPAC824.07.1:pep; SPAC824.07.
GeneIDⁱ	2542447.
KEGGⁱ	spo:SPAC824.07.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CU329670 Genomic DNA. Translation: CAB57337.1.
PIRⁱ	T39108.
RefSeqⁱ	NP_593446.1. NM_001018879.2.

3D structure databases

ProteinModelPortalⁱ	Q9UT36.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

MINTⁱ	MINT-4709494.

MINTⁱ

MINT-4709494.

Proteomic databases

MaxQBⁱ	Q9UT36.

MaxQBⁱ

Q9UT36.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	SPAC824.07.1; SPAC824.07.1:pep; SPAC824.07.
GeneIDⁱ	2542447.
KEGGⁱ	spo:SPAC824.07.

Organism-specific databases

EuPathDBⁱ	FungiDB:SPAC824.07.
PomBaseⁱ	SPAC824.07.

Phylogenomic databases

HOGENOMⁱ	HOG000058041.
InParanoidⁱ	Q9UT36.
KOⁱ	K01069.
OMAⁱ	HVKCLST.
OrthoDBⁱ	EOG092C4YMK.
PhylomeDBⁱ	Q9UT36.

Enzyme and pathway databases

UniPathwayⁱ	UPA00619; UER00676.
Reactomeⁱ	R-SPO-70268. Pyruvate metabolism.

Miscellaneous databases

PROⁱ	Q9UT36.

PROⁱ

Q9UT36.

Family and domain databases

Gene3Dⁱ	3.60.15.10. 1 hit.
InterProⁱ	IPR032282. HAGH_C. IPR001279. Metallo-B-lactamas. [Graphical view]
Pfamⁱ	PF16123. HAGH_C. 1 hit. PF00753. Lactamase_B. 1 hit. [Graphical view]
SMARTⁱ	SM00849. Lactamase_B. 1 hit. [Graphical view]
SUPFAMⁱ	SSF56281. SSF56281. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ

GLO21_SCHPO

Accessionⁱ

Primary (citable) accession number: Q9UT36

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 1, 2000
Last modified:	September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9UT36 (GLO21_SCHPO)

UniProt

Q9UT36 - GLO21_SCHPO

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Probable hydroxyacylglutathione hydrolase C824.07

Select a section on the left to see content.

<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

<p>Describes the metabolic pathway(s) associated with a protein.</p><p><a href='../manual/pathway' target='_top'>More...</a></p>Pathwayi: methylglyoxal degradation

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>Provides information on the location and the topology of the mature protein in the cell.</p><p><a href='../manual/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>Provides information on the tertiary and secondary structure of a protein.</p><p><a href='../manual/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>Contains any relevant information that doesn’t fit in any other defined sections</p><p><a href='../manual/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: methylglyoxal degradation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ