Q9UT35 (GDA1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-diphosphatase
Short name=GDPase
EC=3.6.1.42

Gene names

Name:	gdp1
ORF Names:	SPAC824.08

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol. Ref.1
Catalytic activity	GDP + H2O = GMP + phosphate.
Cofactor	Calcium. Ref.1 Manganese. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.1.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Manganese Metal-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from direct assay. Source: GeneDB_Spombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	guanosine-diphosphatase activity Inferred from direct assay. Source: GeneDB_Spombe metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide diphosphatase activity Inferred from mutant phenotype. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Guanosine-diphosphatase		PRO_0000209918
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	34 – 556	523	Lumenal Potential
Sites
	Active site	256	1	Proton acceptor By similarity
Amino acid modifications
	Glycosylation	372	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UT35 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1D811E3D6A6BBB85	FASTA	556	61,589
	10 20 30 40 50 60 MTPTMKSIAR RKALLIALSI FAVTFILWNG FPGSSNRPLP SSNDEFHYED IELPSGYRSE 70 80 90 100 110 120 GEVVDLLNPK DELEEPLFSE EPLFPVTTSI PTKTAVSKPK IAPTSAAKDV TFSSSIDSDD 130 140 150 160 170 180 CSVAYDNSKP VRQYVLMIDA GSTGSRVHVY QFNNCNPSPK LEEEFFKMIE PGLSSFAGDP 190 200 210 220 230 240 EGAAASLDPL LDYAMENVPE EYRRCSPIAV KATAGLRLTG ESEAKAILKS VRQHLENDYP 250 260 270 280 290 300 FPIVKDGVSI LEGSMEGIYA WITINYLLGT LGGKATHSTV AVMDLGGAST QLVFEPRFAS 310 320 330 340 350 360 DGESLVDGDH KYVLDYNGEQ YELYQHSHLG YGLKEARKLI HKFVLNNAEA LKESLELLGD 370 380 390 400 410 420 STSIIHPCLH LNASLTHPDS KSEASEVVFV GPSLAHLSLQ CRGIAEKALY KDKNCPVRPC 430 440 450 460 470 480 SFNGVHQPKF TETFTDSPIY LISYFYDRMI SLGMPSTFTI EDMKYLANSV CSGPTYWQDA 490 500 510 520 530 540 FSLTDALKEL KEEPEWCLDL NYMISLLSVG YEIPNNRQLH TAKKIDNKEL GWCLGASLSM 550 LSEQNNGWNC NVKEEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of gdp1+ as encoding a GDPase in the fission yeast Schizosaccharomyces pombe." Sanchez R., Franco A., Gacto M., Notario V., Cansado J. FEMS Microbiol. Lett. 228:33-38(2003) [PubMed: 14612233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, SUBCELLULAR LOCATION. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF465240 Genomic DNA. Translation: AAL69974.1. CU329670 Genomic DNA. Translation: CAB57338.1.
PIR	T39109.
RefSeq	NP_593447.1. NM_001018880.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UT35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC824.08.1; SPAC824.08.1:pep; SPAC824.08.
GeneID	2541834.
GenomeReviews	Gene locus gdp1 in contig CU329670_GR.
KEGG	spo:SPAC824.08.
NMPDR	fig|4896.1.peg.3417.
Organism-specific databases
GeneDB_Spombe	SPAC824.08.
Phylogenomic databases
eggNOG	fuNOG05233.
GeneTree	EFGT00050000004211.
HOGENOM	HBG736908.
OMA	EISAFSY.
OrthoDB	EOG447K2K.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001132-MONOMER.
Gene expression databases
ArrayExpress	Q9UT35.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. IPR015605. Guanosine_diphosphatase. [Graphical view]
KO	K01526.
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit. PTHR11782:SF9. Gda1p. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDA1_SCHPO

Accession

Primary (citable) accession number: Q9UT35

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program