Q9UT28 (NNT1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative nicotinamide N-methyltransferase
EC=2.1.1.1

Gene names

Name:	nnt1
ORF Names:	SPAC8F11.09c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Putative nicotinamide N-methyltransferase involved in rDNA silencing and in lifespan determination By similarity.
Catalytic activity	S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NNT1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin silencing at rDNA Inferred from sequence or structural similarity. Source: GeneDB_Spombe nicotinamide metabolic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	nicotinamide N-methyltransferase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Putative nicotinamide N-methyltransferase		PRO_0000096899

Sequences

	Sequence		Length	Mass (Da)
	Q9UT28 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8E16DB39F6173EA0	FASTA	255	28,490
	10 20 30 40 50 60 MADNDFEGFG IFEEPEGFRP STPPPKEVLH TRVIVPNGPE EIKLRLVGSH SLWAHYLWNS 70 80 90 100 110 120 GIELANYIDK NPDTVRAKKV LELGAGAGLP SIVSAFDGAK FVVSTDYPDP ALIDNLEHNV 130 140 150 160 170 180 KQYAEIASKI SAVGYLWGSN IKEVMSNAGF KDNEVFDILL LSDLVFNHTE HSKLIKSCKM 190 200 210 220 230 240 AIEGNPNAVV YVFFTHHRPH LAKKDMIFFD IAQSEGFQIE KILEEKRTPM FEEDPGAPEI 250 RATVHGYKMT IPIPV

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB52170.1.
PIR	T39184.
RefSeq	NP_593958.1. NM_001019385.1.
3D structure databases
ProteinModelPortal	Q9UT28.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UT28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC8F11.09c.1; SPAC8F11.09c.1:pep; SPAC8F11.09c.
GeneID	2543599.
GenomeReviews	Gene locus nnt1 in contig CU329670_GR.
KEGG	spo:SPAC8F11.09c.
NMPDR	fig|4896.1.peg.3928.
Organism-specific databases
GeneDB_Spombe	SPAC8F11.09c.
Phylogenomic databases
eggNOG	fuNOG05731.
GeneTree	EFGT00050000001751.
HOGENOM	HBG397955.
OMA	HLLWNAG.
OrthoDB	EOG4MKRRQ.
Gene expression databases
ArrayExpress	Q9UT28.
Family and domain databases
InterPro	IPR019410. Nicotinamide_N-MeTfrase_put. [Graphical view]
Pfam	PF10294. Methyltransf_16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NNT1_SCHPO

Accession

Primary (citable) accession number: Q9UT28

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program