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Protein

Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

met26

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.1 Publication

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (met26)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi652ZincBy similarity1
Metal bindingi654ZincBy similarity1
Metal bindingi735ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adenine biosynthetic process Source: PomBase
  • cysteine metabolic process Source: PomBase
  • homocysteine metabolic process Source: GO_Central
  • L-methionine biosynthetic process from homoserine via O-acetyl-L-homoserine and cystathionine Source: PomBase

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:met26
ORF Names:SPAC9.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC9.09
PomBaseiSPAC9.09 met26

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to methionine and adenine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000987032 – 764Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182Phosphoserine1 Publication1
Modified residuei441Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UT19
PaxDbiQ9UT19
PRIDEiQ9UT19

PTM databases

iPTMnetiQ9UT19

Interactioni

Protein-protein interaction databases

BioGridi280000, 3 interactors
STRINGi4896.SPAC9.09.1

Structurei

3D structure databases

ProteinModelPortaliQ9UT19
SMRiQ9UT19
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246221
InParanoidiQ9UT19
KOiK00549
OMAiGRNIWKN
OrthoDBiEOG092C0LN1
PhylomeDBiQ9UT19

Family and domain databases

Gene3Di3.20.20.210, 4 hits
HAMAPiMF_00172 Meth_synth, 1 hit
InterProiView protein in InterPro
IPR013215 Cbl-indep_Met_Synth_N
IPR006276 Cobalamin-indep_Met_synthase
IPR002629 Met_Synth_C/arc
IPR038071 UROD/MetE-like_sf
PfamiView protein in Pfam
PF08267 Meth_synt_1, 1 hit
PF01717 Meth_synt_2, 1 hit
PIRSFiPIRSF000382 MeTrfase_B12_ind, 1 hit
SUPFAMiSSF51726 SSF51726, 2 hits
TIGRFAMsiTIGR01371 met_syn_B12ind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UT19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSAVLGFP RIGKNRELKK ATEAYWSGKT SAEELLATAK QLRLEHWKLQ
60 70 80 90 100
KAQGVDIIPS NDFSLYDQIM DHSFSFNVIP PRYRLSGLSS LDTYFAMGRG
110 120 130 140 150
MQRAATADKA AVDVPAGEMV KWFDSNYHFL RPEVSEETDF KLSSTKALDE
160 170 180 190 200
FLEAKEAGII TRPVLVGPVT YLFIAKAAKG SSIKPIELLP KLLPVYVELI
210 220 230 240 250
KKLTEAGAEY IQIDEPILTL DLPQEILASY KEAYETLGKI GKLILTTYFG
260 270 280 290 300
SLQSNADVLK GLPIAGVHVD VVRAPENLDR ALAVLGENQI ISVGVVSGRN
310 320 330 340 350
IWKTDFQKAT AIIEKAISAV GSERVQVASS SSILHIPHSL SGEDQINPEI
360 370 380 390 400
KRWFAFAVEK CAELAILTKA ANDGPASVRA ELEANAADCK ARAESPITNV
410 420 430 440 450
EAVRERQSKV TPQMHERKSP FETRYAKQQA SLKLPLFPTT TIGSFPQTKE
460 470 480 490 500
IRVTRNRFAK GLISQEEYDA FIRKEISDVV KFQEEVGLDV LVHGEPERND
510 520 530 540 550
MVQYFGERME GFVFTVNGWV QSYGSRCVRP PIIVGDVYRP APMTVKESQY
560 570 580 590 600
AQSITSKPMK GMLTAPITIL RWSFPRDDVH DSVQAQQIAL GLRDEVLDLE
610 620 630 640 650
KAGIKVIQCD EPALREGLPL RRAEWDEYLK WAIDAFRLAT AAVQDDTQIH
660 670 680 690 700
SHFCYSDFND IFDAIQRLDA DVVSIENSKS DMKLLNVLSR YTSCIGPGLF
710 720 730 740 750
DIHSPRVPPV SEFKERIDAI VKHVPKDHLW LNPDCGLKTR GWPETTADLK
760
NMIAAAREAR EQYA
Length:764
Mass (Da):85,340
Last modified:May 1, 2000 - v1
Checksum:iE574CDC98A54710A
GO

Sequence cautioni

The sequence BAA13829 differs from that shown. Reason: Frameshift at position 738.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416E → G in BAA13829 (PubMed:9501991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB57427.1
D89167 mRNA Translation: BAA13829.1 Frameshift.
PIRiT39194
T42529
RefSeqiNP_593352.1, NM_001018784.2

Genome annotation databases

EnsemblFungiiSPAC9.09.1; SPAC9.09.1:pep; SPAC9.09
GeneIDi2543585
KEGGispo:SPAC9.09

Similar proteinsi

Entry informationi

Entry nameiMETE_SCHPO
AccessioniPrimary (citable) accession number: Q9UT19
Secondary accession number(s): P78818
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health