Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

met26

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.1 Publication

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (met26)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi652ZincBy similarity1
Metal bindingi654ZincBy similarity1
Metal bindingi735ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adenine biosynthetic process Source: PomBase
  • cysteine metabolic process Source: PomBase
  • homocysteine metabolic process Source: GO_Central
  • L-methionine biosynthetic process from homoserine via O-acetyl-L-homoserine and cystathionine Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:met26
ORF Names:SPAC9.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC9.09.
PomBaseiSPAC9.09. met26.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • extracellular region Source: GO_Central
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to methionine and adenine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000987032 – 764Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182Phosphoserine1 Publication1
Modified residuei441Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UT19.
PRIDEiQ9UT19.

PTM databases

iPTMnetiQ9UT19.

Interactioni

Protein-protein interaction databases

BioGridi280000. 3 interactors.
MINTiMINT-4709347.

Structurei

3D structure databases

ProteinModelPortaliQ9UT19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246221.
InParanoidiQ9UT19.
KOiK00549.
OMAiLESHILW.
OrthoDBiEOG092C0LN1.
PhylomeDBiQ9UT19.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UT19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSAVLGFP RIGKNRELKK ATEAYWSGKT SAEELLATAK QLRLEHWKLQ
60 70 80 90 100
KAQGVDIIPS NDFSLYDQIM DHSFSFNVIP PRYRLSGLSS LDTYFAMGRG
110 120 130 140 150
MQRAATADKA AVDVPAGEMV KWFDSNYHFL RPEVSEETDF KLSSTKALDE
160 170 180 190 200
FLEAKEAGII TRPVLVGPVT YLFIAKAAKG SSIKPIELLP KLLPVYVELI
210 220 230 240 250
KKLTEAGAEY IQIDEPILTL DLPQEILASY KEAYETLGKI GKLILTTYFG
260 270 280 290 300
SLQSNADVLK GLPIAGVHVD VVRAPENLDR ALAVLGENQI ISVGVVSGRN
310 320 330 340 350
IWKTDFQKAT AIIEKAISAV GSERVQVASS SSILHIPHSL SGEDQINPEI
360 370 380 390 400
KRWFAFAVEK CAELAILTKA ANDGPASVRA ELEANAADCK ARAESPITNV
410 420 430 440 450
EAVRERQSKV TPQMHERKSP FETRYAKQQA SLKLPLFPTT TIGSFPQTKE
460 470 480 490 500
IRVTRNRFAK GLISQEEYDA FIRKEISDVV KFQEEVGLDV LVHGEPERND
510 520 530 540 550
MVQYFGERME GFVFTVNGWV QSYGSRCVRP PIIVGDVYRP APMTVKESQY
560 570 580 590 600
AQSITSKPMK GMLTAPITIL RWSFPRDDVH DSVQAQQIAL GLRDEVLDLE
610 620 630 640 650
KAGIKVIQCD EPALREGLPL RRAEWDEYLK WAIDAFRLAT AAVQDDTQIH
660 670 680 690 700
SHFCYSDFND IFDAIQRLDA DVVSIENSKS DMKLLNVLSR YTSCIGPGLF
710 720 730 740 750
DIHSPRVPPV SEFKERIDAI VKHVPKDHLW LNPDCGLKTR GWPETTADLK
760
NMIAAAREAR EQYA
Length:764
Mass (Da):85,340
Last modified:May 1, 2000 - v1
Checksum:iE574CDC98A54710A
GO

Sequence cautioni

The sequence BAA13829 differs from that shown. Reason: Frameshift at position 738.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti416E → G in BAA13829 (PubMed:9501991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB57427.1.
D89167 mRNA. Translation: BAA13829.1. Frameshift.
PIRiT39194.
T42529.
RefSeqiNP_593352.1. NM_001018784.2.

Genome annotation databases

EnsemblFungiiSPAC9.09.1; SPAC9.09.1:pep; SPAC9.09.
GeneIDi2543585.
KEGGispo:SPAC9.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB57427.1.
D89167 mRNA. Translation: BAA13829.1. Frameshift.
PIRiT39194.
T42529.
RefSeqiNP_593352.1. NM_001018784.2.

3D structure databases

ProteinModelPortaliQ9UT19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280000. 3 interactors.
MINTiMINT-4709347.

PTM databases

iPTMnetiQ9UT19.

Proteomic databases

MaxQBiQ9UT19.
PRIDEiQ9UT19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC9.09.1; SPAC9.09.1:pep; SPAC9.09.
GeneIDi2543585.
KEGGispo:SPAC9.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC9.09.
PomBaseiSPAC9.09. met26.

Phylogenomic databases

HOGENOMiHOG000246221.
InParanoidiQ9UT19.
KOiK00549.
OMAiLESHILW.
OrthoDBiEOG092C0LN1.
PhylomeDBiQ9UT19.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.

Miscellaneous databases

PROiQ9UT19.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETE_SCHPO
AccessioniPrimary (citable) accession number: Q9UT19
Secondary accession number(s): P78818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.