Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiamine transporter thi9

Gene

thi9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiamine transporter involved in the cellular uptake of thiamine. Pyrithiamine, oxythiamine, amprolium, and the thiazole part of thiamine have been shown to be also substrates of thi9.1 Publication

Kineticsi

  1. KM=0.4 µM for thiamine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • thiamine transmembrane transport Source: PomBase
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Transport

    Protein family/group databases

    TCDBi2.A.3.4.6. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine transporter thi9
    Gene namesi
    Name:thi9
    ORF Names:SPAC9.10
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome I

    Organism-specific databases

    EuPathDBiFungiDB:SPAC9.10.
    PomBaseiSPAC9.10. thi9.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei98 – 11821HelicalSequence analysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence analysisAdd
    BLAST
    Transmembranei397 – 41721HelicalSequence analysisAdd
    BLAST
    Transmembranei450 – 47021HelicalSequence analysisAdd
    BLAST
    Transmembranei545 – 56521HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • endoplasmic reticulum Source: PomBase
    • endoplasmic reticulum membrane Source: UniProtKB-SubCell
    • integral component of plasma membrane Source: GO_Central
    • medial membrane band Source: PomBase
    • plasma membrane Source: PomBase
    • plasma membrane of cell tip Source: PomBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi81 – 811E → K: Accumulates in the endoplasmic reticulum and punctuate cytoplasmic structures. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 591591Thiamine transporter thi9PRO_0000054169Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei585 – 5851Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9UT18.

    PTM databases

    iPTMnetiQ9UT18.

    Interactioni

    Protein-protein interaction databases

    BioGridi279993. 13 interactions.
    MINTiMINT-4709338.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000160602.
    InParanoidiQ9UT18.
    OMAiSQTESHE.
    OrthoDBiEOG092C1KJF.
    PhylomeDBiQ9UT18.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 2 hits.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9UT18-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSSQISHQD PELGQTSSGS SSIKEKAEPQ LYAGPIDPAR RPDVFQEGFE
    60 70 80 90 100
    DVSVTDDDND NELLRKMGYQ PVLHRSFEFF ESFAASFASL DVVSGVRLTF
    110 120 130 140 150
    SWGISFGGPA AYWSAMLVTG FCSIVTAACL AEICSALPAA GSIYLWAAES
    160 170 180 190 200
    AGPRFGRFVS FLVAWWSTTA WTTFVASITQ STANFIFAEV STFNNPWPTN
    210 220 230 240 250
    DSDVKFRAVQ WIVAEVLLVF TILLNQVPPR YYKWIFKASM LLMFIDYVMN
    260 270 280 290 300
    IIWVPVATSK KPDGFRSAKW VFTETIYDQA GYIKEVDDAN GNPIASLSKI
    310 320 330 340 350
    VPKGWQWCLS YFATAGVIVG YDASGHIAEE TKDASIKAAR GIFYSTVTSF
    360 370 380 390 400
    IVAFSLAILY LFCCPDLDTF TAILYNDNSP QPFVNFYSYL LGRGGHVVMN
    410 420 430 440 450
    VVIILEIFLN GVVSVLACSR LVFAVSRDGV LPFSNWISQV SKTGQPKNAI
    460 470 480 490 500
    TVIYIVSALL LCTILPSAVA FTSLVSAAGA PSFAAYAVLA FCRLFITRDK
    510 520 530 540 550
    FPKGRWSLGW LSKPCLVITL VYNLFALVVN VSPYTYPVTG PSFNYAVVIM
    560 570 580 590
    GGVSIFAIIC TIVIPKSRWV ANRYRYESDS EHSASVKELK V
    Length:591
    Mass (Da):65,070
    Last modified:May 1, 2000 - v1
    Checksum:iFFCCD707AC7849D4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAB57428.1.
    PIRiT39195.
    RefSeqiNP_593353.1. NM_001018785.2.

    Genome annotation databases

    EnsemblFungiiSPAC9.10.1; SPAC9.10.1:pep; SPAC9.10.
    GeneIDi2543578.
    KEGGispo:SPAC9.10.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329670 Genomic DNA. Translation: CAB57428.1.
    PIRiT39195.
    RefSeqiNP_593353.1. NM_001018785.2.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi279993. 13 interactions.
    MINTiMINT-4709338.

    Protein family/group databases

    TCDBi2.A.3.4.6. the amino acid-polyamine-organocation (apc) family.

    PTM databases

    iPTMnetiQ9UT18.

    Proteomic databases

    MaxQBiQ9UT18.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPAC9.10.1; SPAC9.10.1:pep; SPAC9.10.
    GeneIDi2543578.
    KEGGispo:SPAC9.10.

    Organism-specific databases

    EuPathDBiFungiDB:SPAC9.10.
    PomBaseiSPAC9.10. thi9.

    Phylogenomic databases

    HOGENOMiHOG000160602.
    InParanoidiQ9UT18.
    OMAiSQTESHE.
    OrthoDBiEOG092C1KJF.
    PhylomeDBiQ9UT18.

    Miscellaneous databases

    PROiQ9UT18.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 2 hits.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiTHI9_SCHPO
    AccessioniPrimary (citable) accession number: Q9UT18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 14, 2001
    Last sequence update: May 1, 2000
    Last modified: September 7, 2016
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.