Reviewed, UniProtKB/Swiss-Prot Q9USY4 (ABP2_SCHPO)

Names and origin

Protein names

Recommended name:
    ARS-binding protein 2

Gene names

Name:	abp2
ORF Names:	SPBC1861.02

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds, preferentially, to the Maundrell ARS consensus sequence within ARS3002. Ref.1
Subcellular location	Nucleus.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication initiation Inferred by curator. Source: GeneDB_SPombe
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	DNA replication origin binding Ref.1 Inferred from direct assay. Source: GeneDB_SPombe protein binding Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	ARS-binding protein 2		PRO_0000064430
Amino acid modifications
	Modified residue	297	1	Phosphoserine Ref.3
	Modified residue	298	1	Phosphoserine Ref.3
	Modified residue	302	1	Phosphoserine Ref.3
Experimental info
	Mutagenesis	331	1	R → A: 40-fold decrease in DNA-binding. Ref.1
	Mutagenesis	332	1	G → A: Loss of DNA-binding. Ref.1
	Mutagenesis	333	1	R → A: Loss of DNA-binding. Ref.1
	Sequence conflict	260	1	A → P in AAC49930. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9USY4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2860AE683999B9F4	FASTA	527	59,629
	10 20 30 40 50 60 MNFYSLLPSR HDVSADNITE KFCQFCLCCN PWYAGADTRQ LANAFNMIPK SEGQKFEIWV 70 80 90 100 110 120 LFLLVRQYHQ KIINSWSKLV GFLGVERKDE QSTQKIQQYV VRLKRWMSQT HVDAFFDYLL 130 140 150 160 170 180 NKPNPYYLEI PETQPQVRCN VNVTDDLVIK SLRAGLMSHP DVNSSSISTM RTSTSPSNWI 190 200 210 220 230 240 HSASASLDDN THNLGSFVTN PHADSQECPT PQSLLMRASH HMSERGSQQA HQTTPQNHSL 250 260 270 280 290 300 PHPPNMFEPP DEDHQLPSAA NNSHNHDHAF QTAEAVGVAD SIDPDWHQWP DDLRDVSSPK 310 320 330 340 350 360 ESDGLNDSWS RQTNQVETEN VENEAGVPRK RGRPPGARNK IKRLRSEPSV SLTLSISWYE 370 380 390 400 410 420 RFEKLMHAQN TMLRSAFSHV ARLPMETVSQ LLSHYTETIS QYLPPSETSP IPKTPNFKFI 430 440 450 460 470 480 SSTLLALVSS HSEILEASTD RLIWTVHQGP LTATICHAFN LEKAPSMHSL AEAQMIPDVP 490 500 510 520 ISHSNSMEPL DTVSSLKLEI AKLNAALKEK NLELENLKRK IMNAVFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization, and molecular cloning of a protein (Abp2) that binds to a Schizosaccharomyces pombe origin of replication (ars3002)." Sanchez J.P., Murakami Y., Huberman J.A., Hurwitz J. Mol. Cell. Biol. 18:1670-1681(1998) [PubMed: 9488484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 154-171; 302-308; 312-328; 377-384 AND 419-431, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-331; GLY-332 AND ARG-333. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297; SER-298 AND SER-302, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73044 mRNA. Translation: AAC49930.1. CU329671 Genomic DNA. Translation: CAB52738.1.
PIR	T39741.
RefSeq	NP_596719.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9USY4. 1 interaction.
STRING	Q9USY4.
Genome annotation databases
GeneID	2539827.
GenomeReviews	Gene locus abp2 in contig CU329671_GR.
KEGG	spo:SPBC1861.02.
NMPDR	fig|4896.1.peg.2585.
Organism-specific databases
GeneDB_Spombe	SPBC1861.02.
Phylogenomic databases
OrthoDB	EOG98964J
Gene expression databases
ArrayExpress	Q9USY4.
Family and domain databases
InterPro	IPR018562. ARS_binding_protein-2. [Graphical view]
Pfam	PF09441. Abp2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABP2_SCHPO

Accession

Primary (citable) accession number: Q9USY4
Secondary accession number(s): P78967

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)