Q9USU8 (NGG1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromatin-remodeling complexes subunit ngg1

Alternative name(s):
Kinesin-associated protein 1

Gene names

Name:	ngg1
Synonyms:	ada3, kap1
ORF Names:	SPBC28F2.10c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription regulator. Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA, SLIK and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of a subset of genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3. ADA preferentially acetylates nucleosomal histones H3 (at 'Lys-14' and 'Lys-18') and H2B.
Subunit structure	Component of the SAGA complex, the SALSA complex, the ADA complex and the SLIK complex which is probably a subcomplex of SAGA.
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the NGG1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cadmium ion Inferred from mutant phenotype PubMed 18684775. Source: PomBase chromatin remodeling Inferred from sequence orthology. Source: PomBase detoxification of cadmium ion Inferred from mutant phenotype PubMed 18684775. Source: PomBase histone acetylation Inferred from sequence orthology. Source: PomBase regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 19056896. Source: PomBase transcription from RNA polymerase II promoter Inferred by curator. Source: PomBase
Cellular_component	SAGA complex Inferred from direct assay PubMed 19056896. Source: PomBase nuclear chromatin Inferred by curator. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Chromatin-remodeling complexes subunit ngg1		PRO_0000353803
Regions
	Coiled coil	417 – 476	60	Potential
Amino acid modifications
	Modified residue	120	1	Phosphoserine Ref.4
	Modified residue	144	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9USU8 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F037D741B0A78403	FASTA	551	62,407
	10 20 30 40 50 60 MSSEQQNEAD SKPAVIPQCF KIENQYETFS RLSETSTPGV VPSVSTLWRL LFELQKMIEC 70 80 90 100 110 120 EPSCVEYFRQ RKEELESHVD SEIETSKDES SVNKVEEKVE EFKEDNVEQE IKQKRSLSES 130 140 150 160 170 180 PQESMLEKVS KKPKVSEAHN EEISPENVET IENELDLPVK GKDEQTTGLV YKNANDLLTG 190 200 210 220 230 240 SLLSFIVDDS FSYEQKKKLL CVDSFPTSDV RSLVAGTPAT DDFSHNKPNN QISISTFYSS 250 260 270 280 290 300 LDPYFRAFND DDIAFLKKGF DVSSSYNIPP LGERYYDLTP EDEMTNLCAN SIYQNLQTSA 310 320 330 340 350 360 QGSLEAFNEA DTVSEEVRCG PLTERLMASL IPCYTQNDEE QKPSIAVGEF AETDSGSEKS 370 380 390 400 410 420 KIGTSIDGIE SGNNEYTEQP DIQESSLSIC EDRLRYTLKQ LGILYDGDVD WSKRQDDEIS 430 440 450 460 470 480 ATLRSLNARL KVVSDENEKM RNALLQMLPE EMAFQEFQNV MDDLDKQIEQ AYVKRNRSLK 490 500 510 520 530 540 VKKKRIVTDK IGSSATSGSF PVIKSLMDKR SMWLEKLQPL FQDKLTQHLG SPTSIFNDLS 550 DHTTSNYSTS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The studies of the kinesin-associated proteins in Schizosaccharomyces pombe." Jeong J.W., Kim H.B. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120 AND SER-144, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF351206 mRNA. Translation: AAK17897.1. CU329671 Genomic DNA. Translation: CAB57939.1.
PIR	T40053.
RefSeq	NP_595671.1. NM_001021566.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC28F2.10c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC28F2.10c.1; SPBC28F2.10c.1:pep; SPBC28F2.10c.
GeneID	2540436.
KEGG	spo:SPBC28F2.10c.
Organism-specific databases
PomBase	SPBC28F2.10c.
Phylogenomic databases
eggNOG	NOG265778.
KO	K11315.
OrthoDB	EOG47M56J.
Family and domain databases
InterPro	IPR019340. Histone_AcTrfase_su3. [Graphical view]
Pfam	PF10198. Ada3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801563.

Entry information

Entry name

NGG1_SCHPO

Accession

Primary (citable) accession number: Q9USU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program