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Protein

Dimethyladenosine transferase

Gene

dim1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1779)/adenine(1780) in 18S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1779)/N(6)-dimethyladenine(1780) in 18S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei33 – 331S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei58 – 581S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei79 – 791S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei107 – 1071S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei122 – 1221S-adenosyl-L-methioninePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethyladenosine transferase (EC:2.1.1.183)
Alternative name(s):
18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase
18S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene namesi
Name:dim1
ORF Names:SPBC336.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC336.02.
PomBaseiSPBC336.02.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: GO_Central
  • mitotic spindle Source: PomBase
  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Dimethyladenosine transferasePRO_0000101462Add
BLAST

Proteomic databases

MaxQBiQ9USU2.

Interactioni

Protein-protein interaction databases

MINTiMINT-4708798.

Structurei

3D structure databases

ProteinModelPortaliQ9USU2.
SMRiQ9USU2. Positions 30-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000227963.
InParanoidiQ9USU2.
KOiK14191.
OMAiGNMTIKI.
OrthoDBiEOG7WQ83Z.
PhylomeDBiQ9USU2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9USU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKIRVRNNN AASDAEVRNT VFKFNKDFGQ HILKNPLVAQ GIVDKADLKQ
60 70 80 90 100
SDTVLEVGPG TGNLTVRMLE KARKVIAVEM DPRMAAEITK RVQGTPKEKK
110 120 130 140 150
LQVVLGDVIK TDLPYFDVCV SNTPYQISSP LVFKLLQQRP APRAAILMFQ
160 170 180 190 200
REFALRLVAR PGDPLYCRLS ANVQMWAHVK HIMKVGKNNF RPPPLVESSV
210 220 230 240 250
VRIEPKNPPP PLAFEEWDGL LRIVFLRKNK TIGACFKTSS IIEMVENNYR
260 270 280 290 300
TWCSQNERMV EEDFDVKSLI DGVLQQCNLQ DARASKCGQT EFLSLLHAFH

QVGVHFA
Length:307
Mass (Da):34,678
Last modified:May 1, 2000 - v1
Checksum:iE2F4543EB190B94A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701S → P in CAA92585 (Ref. 1) Curated
Sequence conflicti290 – 2923TEF → QS in CAA92585 (Ref. 1) Curated
Sequence conflicti298 – 2981A → P in CAA92585 (Ref. 1) Curated
Sequence conflicti304 – 3074VHFA → RSFWLMGQDGVFH in CAA92585 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68293 Genomic DNA. Translation: CAA92585.1.
CU329671 Genomic DNA. Translation: CAB58154.1.
PIRiT40240.
T43249.
RefSeqiNP_596122.1. NM_001022040.2.

Genome annotation databases

EnsemblFungiiSPBC336.02.1; SPBC336.02.1:pep; SPBC336.02.
GeneIDi2540247.
KEGGispo:SPBC336.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68293 Genomic DNA. Translation: CAA92585.1.
CU329671 Genomic DNA. Translation: CAB58154.1.
PIRiT40240.
T43249.
RefSeqiNP_596122.1. NM_001022040.2.

3D structure databases

ProteinModelPortaliQ9USU2.
SMRiQ9USU2. Positions 30-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4708798.

Proteomic databases

MaxQBiQ9USU2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC336.02.1; SPBC336.02.1:pep; SPBC336.02.
GeneIDi2540247.
KEGGispo:SPBC336.02.

Organism-specific databases

EuPathDBiFungiDB:SPBC336.02.
PomBaseiSPBC336.02.

Phylogenomic databases

HOGENOMiHOG000227963.
InParanoidiQ9USU2.
KOiK14191.
OMAiGNMTIKI.
OrthoDBiEOG7WQ83Z.
PhylomeDBiQ9USU2.

Miscellaneous databases

PROiQ9USU2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and comparative analysis of the SpDIM1 gene from Schizosaccharomyces pombe."
    Housen I., Demonte D., Lafontaine D., Vandenhaute J.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiDIM1_SCHPO
AccessioniPrimary (citable) accession number: Q9USU2
Secondary accession number(s): Q9Y7V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.