Q9USR3 (STT4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase stt4
Short name=PI4-kinase
Short name=PtdIns-4-kinase
EC=2.7.1.67

Gene names

Name:	stt4
ORF Names:	SPBC577.06c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1877 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from sequence or structural similarity. Source: GeneDB_Spombe phosphatidylethanolamine biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe mitochondrion Inferred from sequence or structural similarity. Source: GeneDB_Spombe plasma membrane Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1877	1877	Phosphatidylinositol 4-kinase stt4		PRO_0000314092
Regions
	Domain	1305 – 1491	187	PIK helical
	Domain	1619 – 1875	257	PI3K/PI4K
	Region	1492 – 1625	134	Pleckstrin homology (PH) domain conferring phosphoinositide binding specificity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9USR3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: AD4577CC2D4EC5BD	FASTA	1,877	213,963
	10 20 30 40 50 60 MDCIDKSIRR ESLRKLGCLP DHGWNLFVQS SNRYSKLLEA DSKLFKAQRI EDTVLSICEV 70 80 90 100 110 120 TLENKASENA LTLFQQLRFY LLNYLFLEDT SEYKSVLCSL DESVYPNLLP LTANICPCIL 130 140 150 160 170 180 KALLALVRQH RELSSDIVKF LNSIFLALTR ILNTPHSSNN NPTHTKTTIF EKVFYASHLG 190 200 210 220 230 240 FFFEEIADAC ISALPQDACL WAMEVCNTIL VSDLPCKLID LHNDSFLHHS NYYAGFGLSY 250 260 270 280 290 300 YLYLSSMKAY INSSGCLWYE DATSFWDIVK HRPSSPEEKP FLTQFQSILN LSDKFLSKTG 310 320 330 340 350 360 TLRLQEKLGT SCNLFFVLVL KLALLSSSYI GFVPYTYSDW INVLLGSISE DPELLPHLIE 370 380 390 400 410 420 LMASLACVFP THFQFAMKRL RLIAIYAPRF DDGNVTYAQL ASRKLAQLFN YSSRDTAITS 430 440 450 460 470 480 IYQFANLLSP ADTPDSYLAA PKERLSISDN MSSSSSQTAT VNTISNYLNV IDSVREIALT 490 500 510 520 530 540 VNDEKIYGLA ISLLIQKFSR KFDSRVSTEL VRTLADISTK ANDRDFSILV QFYSIQNKML 550 560 570 580 590 600 VKNGDEALTI AICDSRCLIA RGTESNSFKR SDLLKSLLEE IIHIGIVRDT PAPELKGYYF 610 620 630 640 650 660 GMSKAVQALV ECVAGTDWSS LPKEEMYPAL FRDMWFTLVI NRFRYSVDLG ETLEVDLENI 670 680 690 700 710 720 AKNSPLLVFE DFGSNFESNL ELNTVLRTHI EHSVISQIKS ELLVRVPNID LKPLSTSEIC 730 740 750 760 770 780 FLSAVLLLES LRCKSNKLSA LVEYLLDPSL RDSQLPQSIR AIALYNLTSF VESLSKERRV 790 800 810 820 830 840 ASSTIDEFQK LLCLCCNRVD CVRQLALECM NYIMETLPHL LGIKEILFSV LELSSLLWKA 850 860 870 880 890 900 RTEECTDQYV PQLLYKSNKL NLSVILSDIY SCREEVLFQF NRHARSWIQN SSKAIPYQVK 910 920 930 940 950 960 NLLESYLADF VDFDDLEVVE LGRSLAVELG TRIVSSDRDN FVLPAFGNWT PDTSSEFMAE 970 980 990 1000 1010 1020 YTIRQRYSHV DNSILNIEGD MSTDRIDLIL NEKSKLFETH LAALKSLEDD ILQGNTVSPQ 1030 1040 1050 1060 1070 1080 RLRNEVRKAA AHAVQEPIFQ FSVLARKIVR IPFLDFSPSS FKLGITLWNW MMNQVPSFSS 1090 1100 1110 1120 1130 1140 FLISNIIRNW KNEIVTEKRG YFSTAKSKSP LALPMTYSPT ERASFLSYKN KVMSQMIPHL 1150 1160 1170 1180 1190 1200 LLLQLIAGNF EGFWYGDRQT AKLIVHFMKF VLKKITSMEV NLNVLTRELH FKFVSFGLRI 1210 1220 1230 1240 1250 1260 AENLLNSPLG SRFYNLCVDA GLCWFSGMPN WTYGADKLHV AAEISVMRSL RDKLDSFLLR 1270 1280 1290 1300 1310 1320 YPLKVSTTLK QKLLIILLNN EMYMLYTWLT PVLHGRNVRM VEPIPDSDAA SSPITLEMLQ 1330 1340 1350 1360 1370 1380 VAWNVSPNIV LYAPKRFQNA PLKQMALNFV IANPFTSVKH EVALEYLFEH YPSGEFPIDR 1390 1400 1410 1420 1430 1440 KFILYWEPMY PVSGPVMFMP NVKWNPQLLQ YTMRSMESYP VSVTFFYVPQ IVQSLRYDSM 1450 1460 1470 1480 1490 1500 GYAESFILET SGTSQLFAHQ ILWNMKANLY KDEAATVPDS IKPILDRVMD KMINSLSGED 1510 1520 1530 1540 1550 1560 KQFYEREFTF FNEVTSISGK LKPFIRKSKP EKKAKIDEEM KKIKLDVGVY LPSNPDGVIV 1570 1580 1590 1600 1610 1620 GIDRKSGKPL QSHAKAPFMA TFKIRKEKLV DADPEELAVN GTEEEAGDSA KKQTYEVWQS 1630 1640 1650 1660 1670 1680 AIFKVGDDCR QDVLTLQLIA MFKNIFNSVG LDVYLFPYRV TATNPGCGVI DVLPNCISRD 1690 1700 1710 1720 1730 1740 MLGREAVNGL YDYFRTKFGD EDSIAFQKAR SNFVQSMAAY SVITYLLQFK DRHNGNIMID 1750 1760 1770 1780 1790 1800 DQGHILHIDF GFIFDIAPGG ITFESAPFKL TTEMIAVMGG SNKSQPFQWF QELCVKAFLA 1810 1820 1830 1840 1850 1860 CRPYAHYICQ AVEVMLDSGL PCFKGQLTIT HCLERFALNL NERQASTFML HLIEQSYANK 1870 RTLMYDQFQK ATNGIPY

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB54814.1.
PIR	T40550.
RefSeq	NP_595304.1. NM_001021211.1.
3D structure databases
HSSP	HSSP built from PDB template 1E8Y based on UniProtKB P48736.
ProteinModelPortal	Q9USR3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9USR3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC577.06c.1; SPBC577.06c.1:pep; SPBC577.06c.
GeneID	2540883.
GenomeReviews	Gene locus stt4 in contig CU329671_GR.
KEGG	spo:SPBC577.06c.
NMPDR	fig|4896.1.peg.1170.
Organism-specific databases
GeneDB_Spombe	SPBC577.06c.
Phylogenomic databases
eggNOG	fuNOG06080.
GeneTree	EFGT00050000002040.
OMA	CGVIDVL.
OrthoDB	EOG4GF6P8.
Gene expression databases
ArrayExpress	Q9USR3.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
KO	K00888.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STT4_SCHPO

Accession

Primary (citable) accession number: Q9USR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program