Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diphthine--ammonia ligase

Gene

mug71

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (eft201 or eft202) to diphthamide (By similarity). Has a role in meiosis.By similarity1 Publication

Catalytic activityi

ATP + diphthine-[translation elongation factor 2] + NH3 = AMP + diphosphate + diphthamide-[translation elongation factor 2].

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine--ammonia ligase (EC:6.3.1.14)
Alternative name(s):
Diphthamide synthase
Diphthamide synthetase
Gene namesi
Name:mug71
ORF Names:SPBC577.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC577.12.
PomBaseiSPBC577.12.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Diphthine--ammonia ligasePRO_0000278564Add
BLAST

Proteomic databases

MaxQBiQ9USQ7.

Interactioni

Protein-protein interaction databases

BioGridi277240. 14 interactions.
MINTiMINT-4708505.

Structurei

3D structure databases

ProteinModelPortaliQ9USQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the Diphthine--ammonia ligase family.Curated
In the C-terminal section; belongs to the RutC family.Curated

Phylogenomic databases

HOGENOMiHOG000247962.
InParanoidiQ9USQ7.
KOiK06927.
OMAiIVLVHLY.
OrthoDBiEOG092C4KTI.
PhylomeDBiQ9USQ7.

Family and domain databases

Gene3Di3.30.1330.40. 2 hits.
3.40.50.620. 1 hit.
InterProiIPR002761. Diphthami_syn_dom.
IPR030662. DPH6/MJ0570.
IPR013813. Endoribo_LPSP/chorism_mut-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR006175. YjgF/YER057c/UK114.
[Graphical view]
PANTHERiPTHR12196. PTHR12196. 1 hit.
PfamiPF01902. Diphthami_syn_2. 1 hit.
PF01042. Ribonuc_L-PSP. 2 hits.
[Graphical view]
SUPFAMiSSF55298. SSF55298. 2 hits.
TIGRFAMsiTIGR00290. MJ0570_dom. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9USQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLGLISGG KDSCFNLMHC VSLGHEVVAL ANLHPEDGKD EIDSFMYQSV
60 70 80 90 100
GHDVIPLYAE CFDLPLYREK IGGQSINQNL DYQFTEKDET EDLYRLIKRV
110 120 130 140 150
LTNHPDLEAV STGAILSTYQ RTRVENVCKR LGLKSLSFLW QKDQEKLLND
160 170 180 190 200
MVVSGLNAIL IKVAAIGLTR KDLGKSLAEM QDKLLTLNKK FELHPCGEGG
210 220 230 240 250
EYETLVLDCP LFKKRIVLTD KEVVEHSSGE VCYLKVKACV KDKPEWQPIS
260 270 280 290 300
LKSELVPNEE LLGEEYSHIY HTISKKYELI DDQEETPTSL IPIPLRESAF
310 320 330 340 350
QQKKGSFLVL GNVVATKGSY NTFQGEAESA INNLNELLGT YGYSNKNVYF
360 370 380 390 400
VTVILSSMSK FAEFNSVYNK YFDFTNPPSR SCVAAPLASE YRIVMSCIVG
410 420 430 440 450
DVTEKRALHV QGQSYWAPAN IGPYSQSICA NGVVFISGQI GLIPSVMELK
460 470 480 490 500
LHDKIFEMVL ALQHANRVAK AMRVGSLIAC LAYVCDSRDA DCVVKIWSEY
510 520 530 540 550
TKNTGESSPV LVALVDALPR NASVEWQLLY NDSSCDVPLL SSLVTNQTLF
560 570 580 590 600
GSDTAWDVAL LNQNGLRMES SFIHHEHPSA YAIVLNNAFP NSQLLHVRYT

ARDQNV
Length:606
Mass (Da):67,624
Last modified:May 1, 2000 - v1
Checksum:i059CE8F3A651F599
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB54820.1.
PIRiT40556.
RefSeqiNP_595310.1. NM_001021217.2.

Genome annotation databases

EnsemblFungiiSPBC577.12.1; SPBC577.12.1:pep; SPBC577.12.
GeneIDi2540717.
KEGGispo:SPBC577.12.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB54820.1.
PIRiT40556.
RefSeqiNP_595310.1. NM_001021217.2.

3D structure databases

ProteinModelPortaliQ9USQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277240. 14 interactions.
MINTiMINT-4708505.

Proteomic databases

MaxQBiQ9USQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC577.12.1; SPBC577.12.1:pep; SPBC577.12.
GeneIDi2540717.
KEGGispo:SPBC577.12.

Organism-specific databases

EuPathDBiFungiDB:SPBC577.12.
PomBaseiSPBC577.12.

Phylogenomic databases

HOGENOMiHOG000247962.
InParanoidiQ9USQ7.
KOiK06927.
OMAiIVLVHLY.
OrthoDBiEOG092C4KTI.
PhylomeDBiQ9USQ7.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-SPO-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

PROiQ9USQ7.

Family and domain databases

Gene3Di3.30.1330.40. 2 hits.
3.40.50.620. 1 hit.
InterProiIPR002761. Diphthami_syn_dom.
IPR030662. DPH6/MJ0570.
IPR013813. Endoribo_LPSP/chorism_mut-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR006175. YjgF/YER057c/UK114.
[Graphical view]
PANTHERiPTHR12196. PTHR12196. 1 hit.
PfamiPF01902. Diphthami_syn_2. 1 hit.
PF01042. Ribonuc_L-PSP. 2 hits.
[Graphical view]
SUPFAMiSSF55298. SSF55298. 2 hits.
TIGRFAMsiTIGR00290. MJ0570_dom. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPH6_SCHPO
AccessioniPrimary (citable) accession number: Q9USQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.