Q9USQ6 (SYJ2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol-1,4,5-trisphosphate 5-phosphatase 2
EC=3.1.3.36

Alternative name(s):
Synaptojanin-like protein 2

Gene names

Name:	syj2
ORF Names:	SPBC577.13

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	889 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Controls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate. Involved in distinct membrane trafficking and signal transduction pathways. Highly active against a range of soluble and lipid inositol phosphates. Active in dephosphorylating the 5-position of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 and to a lesser extent Ins(1,4,5,6)P4 By similarity.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm Ref.3.
Induction	By stress. Ref.2
Sequence similarities	Belongs to the synaptojanin family. In the central section; belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. Contains 1 SAC domain.

Ontologies

Keywords
Biological process	Lipid metabolism Protein transport Transport
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to stress Inferred from expression pattern. Source: GeneDB_Spombe endocytosis Inferred from sequence or structural similarity. Source: GeneDB_Spombe inositol lipid-mediated signaling Inferred by curator. Source: GeneDB_Spombe phosphatidylinositol dephosphorylation Inferred from sequence or structural similarity. Source: GeneDB_Spombe protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	inositol-polyphosphate 5-phosphatase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 889	889	Inositol-1,4,5-trisphosphate 5-phosphatase 2		PRO_0000359407
Regions
	Domain	171 – 484	314	SAC

Sequences

	Sequence		Length	Mass (Da)
	Q9USQ6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A22120E274337F99	FASTA	889	101,708
	10 20 30 40 50 60 MQCYLKRKER SVAFITEKYA AIIQKPSVKD SDCIITFTCI SLEEFEKIKE YELLFGGEKI 70 80 90 100 110 120 LGTLGLFNYV CEDMKEGVFL CVARKAKLVL RIGNRDPISK LENVSFISLD QELWDEELFE 130 140 150 160 170 180 PMPRNSSPSS TFSTSTSDLN NIEKDNLVNS EYSSKYSSTT RIYPYHSLSQ LTDLLTDGSF 190 200 210 220 230 240 YVSTNISCFS RFQSEQPYGP KDIYCWNRFL ITELDNHFSE AHILEKFPNL LLHCFRGYVE 250 260 270 280 290 300 RMFIDSMSMS IISKVSSYGS RQTYPPSGID DSSYCSMFVE TEFIVEIAQT VFSFVQVRGT 310 320 330 340 350 360 VPCFWEEQFS SWYGPSISFL RSSQASQSLF NFHFSKLYKA YGDIYVIDLL QTKGFEASLY 370 380 390 400 410 420 EAYKHHLMLL PFPAVVKKFH FTPDPARPDI DPRLETDLAD DLKEMGYTQK SIENDMLESF 430 440 450 460 470 480 QKGVFRINDL DCLGRTNVIQ YQISRLVLKD IFFNLQIGVT FNILEYLRHL WSNNGDAIAK 490 500 510 520 530 540 LITGVGSIGS SATRRGRKSI AGSLSDISKS FGRMYVGRYP DVESQNAILL LLGCFPNQSP 550 560 570 580 590 600 VLISESVSSY IQGVLRQRRS EYRVERDFSI FSSTFNANGK VPSTDEFKRL LLPFGERTSA 610 620 630 640 650 660 YDLYVVAVQE IITLNMSHLV SSSNQKLRIW EEKILMILNS RDSNNKYMLI SSIQMAGVFL 670 680 690 700 710 720 GVFIRKDDHL VVSKVTKTTR KTGFGGFSAN KGAVAIEMNV CDSDFCFVSS HFAPKVNNIS 730 740 750 760 770 780 ERNMEYTSIS DNLVFPSGMK IYDHTNILWM GDFNYRIDSD NEEVRKLVEL DDLDKLASYD 790 800 810 820 830 840 QLCTEMKKGT VFHGLVEPQL TFLPTYKFDN GTNDYDTSDK QRVPSWTDRI LATKSFTRNC 850 860 870 880 YKSCDIRCSD HRPVFATFSL KIFSTCMDKK AGIIRDARIA YIKSKNSNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Global transcriptional responses of fission yeast to environmental stress." Chen D., Toone W.M., Mata J., Lyne R., Burns G., Kivinen K., Brazma A., Jones N., Baehler J. Mol. Biol. Cell 14:214-229(2003) [PubMed: 12529438] [Abstract] Cited for: INDUCTION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB54821.1.
PIR	T40557.
RefSeq	NP_595311.1. NM_001021218.1.
3D structure databases
HSSP	HSSP built from PDB template 1I9Z based on UniProtKB O43001.
ProteinModelPortal	Q9USQ6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC577.13.1; SPBC577.13.1:pep; SPBC577.13.
GeneID	2541098.
GenomeReviews	Gene locus syj2 in contig CU329671_GR.
KEGG	spo:SPBC577.13.
NMPDR	fig|4896.1.peg.1177.
Organism-specific databases
GeneDB_Spombe	SPBC577.13.
Phylogenomic databases
eggNOG	fuNOG06107.
GeneTree	EFGT00050000000216.
OrthoDB	EOG498Z9T.
Gene expression databases
ArrayExpress	Q9USQ6.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR002013. Syja_N. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. PF02383. Syja_N. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
PROSITE	PS50275. SAC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYJ2_SCHPO

Accession

Primary (citable) accession number: Q9USQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program