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Protein

NASP-related protein sim3

Gene

sim3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Histone H3 and H3-like CENP-A-specific chaperone. Promotes delivery and incorporation of CENP-A in centromeric chromatin, probably by escorting nascent CENP-A to CENP-A chromatin assembly factors. Required for central core silencing and normal chromosome segregation.1 Publication

GO - Biological processi

  • CENP-A containing nucleosome assembly Source: PomBase
  • chromatin maintenance Source: PomBase
  • histone exchange Source: PomBase
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
NASP-related protein sim3
Alternative name(s):
CENP-A escort protein sim3
Silencing in the middle of the centromere protein 3
Gene namesi
Name:sim3
ORF Names:SPBC577.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC577.15c.
PomBaseiSPBC577.15c. sim3.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: PomBase
  • nucleus Source: PomBase
  • protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81G → E in sim3-143; Reduces interaction with CENP-A and causes abnormal mitotic phenotypes, including hypercondensed chromatin, lagging chromosomes in anaphase, and unequal segregation of chromosomes. 1 Publication1
Mutagenesisi207E → K in sim3-205; Reduces interaction with CENP-A and causes abnormal mitotic phenotypes, including hypercondensed chromatin, lagging chromosomes in anaphase, and unequal segregation of chromosomes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003633811 – 396NASP-related protein sim3Add BLAST396

Proteomic databases

MaxQBiQ9USQ4.
PRIDEiQ9USQ4.

Interactioni

Subunit structurei

Interacts with cnp1, hht1, hht2 and hht3; has a preference for CENP-A (cnp1) over histone H3 (hht1/2/3).1 Publication

Protein-protein interaction databases

BioGridi277411. 13 interactors.
MINTiMINT-4708488.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati32 – 65TPR 1Add BLAST34
Repeati89 – 122TPR 2Add BLAST34
Repeati199 – 232TPR 3Add BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili267 – 329Sequence analysisAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi390 – 395Poly-Lys6

Sequence similaritiesi

Belongs to the NASP family.Curated
Contains 3 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

InParanoidiQ9USQ4.
KOiK11372.
OMAiDFEVAWE.
OrthoDBiEOG092C4G31.
PhylomeDBiQ9USQ4.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR019544. Tetratricopeptide_SHNi-TPR_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF10516. SHNi-TPR. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9USQ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDTKTLEN SKGNSATDAD TKNPSSSDSR AIEQLVTQGN MAYAQKNYEE
60 70 80 90 100
AVDKYGQALM QSESIHGSES LENRNVLWLY GKSLFQIAIE NSQVLGNALG
110 120 130 140 150
AKESVSQATE SFEEPEAIGS FTFSGQKIEN KYTVNEENSS IAHPEKESEE
160 170 180 190 200
KETNEASPAS EEDEDDFNVA WEVLDLTRVM QSKAVDAYPD SKDEKIRLAD
210 220 230 240 250
IYDLLGELSL EIENFSQASQ DLKTALEWKE KVYNVSNNTL LSEAHYKLAL
260 270 280 290 300
ALEFTNPEDP SNKSRACEHV EKAAEILKNV LNERENEVTD KKGKGKQKAE
310 320 330 340 350
ESTLTSDLEN LREMLSELEQ KTLDLKHGAP SLEEAVMSKM HESSLLSKDS
360 370 380 390
SSLAQAVAEA VKNANDLGGL VKRKRTKQEV TSSSQKEGPK DKKKKD
Length:396
Mass (Da):43,903
Last modified:May 1, 2000 - v1
Checksum:i2077DFC3449FC270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB54823.1.
PIRiT40559.
RefSeqiNP_595313.1. NM_001021220.2.

Genome annotation databases

EnsemblFungiiSPBC577.15c.1; SPBC577.15c.1:pep; SPBC577.15c.
GeneIDi2540895.
KEGGispo:SPBC577.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB54823.1.
PIRiT40559.
RefSeqiNP_595313.1. NM_001021220.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277411. 13 interactors.
MINTiMINT-4708488.

Proteomic databases

MaxQBiQ9USQ4.
PRIDEiQ9USQ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC577.15c.1; SPBC577.15c.1:pep; SPBC577.15c.
GeneIDi2540895.
KEGGispo:SPBC577.15c.

Organism-specific databases

EuPathDBiFungiDB:SPBC577.15c.
PomBaseiSPBC577.15c. sim3.

Phylogenomic databases

InParanoidiQ9USQ4.
KOiK11372.
OMAiDFEVAWE.
OrthoDBiEOG092C4G31.
PhylomeDBiQ9USQ4.

Miscellaneous databases

PROiQ9USQ4.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR019544. Tetratricopeptide_SHNi-TPR_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF10516. SHNi-TPR. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSIM3_SCHPO
AccessioniPrimary (citable) accession number: Q9USQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.