Reviewed, UniProtKB/Swiss-Prot Q9USQ2 (ALP6_SCHPO)

Names and origin

Protein names

Recommended name:
    Spindle pole body component alp6
Alternative name(s):
    Altered polarity protein 6

Gene names

Name:	alp6
ORF Names:	SPBC428.20c, SPBC902.01c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	832 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the gamma tubule complex that is required for the regulation of both interphase microtubules and mitotic bipolar spindles.
Subcellular location	Cytoplasm › cytoskeleton › spindle pole body. Note: And the microtubule organizing center (MTOC).
Sequence similarities	Belongs to the GCP family.
Sequence caution	The sequence BAA94097.1 differs from that shown. Reason: Frameshift at position 1.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	M/G1 transition of mitotic cell cycle Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic microtubule organization Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_SPombe microtubule nucleation Non-traceable author statement. Source: GeneDB_SPombe mitotic cell cycle spindle assembly checkpoint Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe mitotic metaphase/anaphase transition Inferred by curator. Source: GeneDB_SPombe nuclear migration along microtubule Non-traceable author statement. Source: GeneDB_SPombe spindle assembly Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe equatorial microtubule organizing center Ref.1 Inferred from direct assay. Source: GeneDB_SPombe gamma-tubulin complex Ref.1 Traceable author statement. Source: GeneDB_SPombe microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: GeneDB_SPombe spindle pole body Ref.1 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 832	832	Spindle pole body component alp6		PRO_0000078133
Amino acid modifications
	Modified residue	286	1	Phosphothreonine Ref.3
Experimental info
	Sequence conflict	665 – 675	11	Missing in CAA22295. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9USQ2-1 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: C92771C3DBF5C01A	FASTA	832	95,997
	10 20 30 40 50 60 MSEIHVKTAL SRLADKYLQN SKNPSVPYTI ETIVSFFQEI IHSISPDTFQ LDIDDILYKI 70 80 90 100 110 120 YSKIPPEENN DALFSKLSNL VSRLKSQTVI HNKSQILYFL YLLSPISQSS RDVSSHLLDE 130 140 150 160 170 180 SISNPINIPS TEVESSNFGQ TRYDQVPENP QITDWDEGLE NESSISIAHD SSRLNRSTET 190 200 210 220 230 240 SSVQHTLITE ADLLSSISYV LQGISTEYVQ FKNELALLSK RIPVQYLLQM RALSETGLLY 250 260 270 280 290 300 QELKVFSNYD PSVSQSIDGD NVSKAFINDQ SLALQSLKSV ISKELTNFLA LIASLDSQIR 310 320 330 340 350 360 ADASLEKPMV TIRRCIAWTQ VAKLKLRILS SVVNDNMNQE NKKRLIQVVS KYNVHGDPLI 370 380 390 400 410 420 QELSDKILTE ITGPLYEMIE NWIYKGELVD PYQEFFVKEK NGSESHDHQG QGDVVWKGKY 430 440 450 460 470 480 FLDKELIPSF LSEELVDKIF LIGKSLNFAR YGCGDFDWAQ EHYQKLVKKL SYRDPHSLET 490 500 510 520 530 540 VVDKAYTESI NHLVYLMEEV FHLTDHLKAI KKYLLLGQGD FVDLLMESLG NSLDQPANTL 550 560 570 580 590 600 FRHNLTASLE SAIRSSNASY EPEYVLKRLD ARLLELSHGE TGWDVFTLEY KVDSPINVII 610 620 630 640 650 660 TPYCSRQYLK IFNFLWRLKR IEFALAHSWR RVNLGERNVF RNLDYTKFEW HFVSCHLAEM 670 680 690 700 710 720 IHFVCQLQYY ILFEVIEISW QELQLAMEKP NATLDTYIEA HEKYVTSITH KGLLGGGKSR 730 740 750 760 770 780 NEDSFLHQLH DILKVILNFH DAIELLYNFS CSLSNRIRIN VPISTDALAA QYTPIKNELS 790 800 810 820 830 NFTEEFQVRL QKLLHGLASH KDPEMRFLSV RLNYNEFYVS HRRRHDKDVT SQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint." Vardy L., Toda T. EMBO J. 19:6098-6111(2000) [PubMed: 11080156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-286, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB040811 Genomic DNA. Translation: BAA94097.1. Frameshift. CU329671 Genomic DNA. Translation: CAA22295.1.
PIR	T50382.
RefSeq	XP_001713121.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9USQ2.
Genome annotation databases
GeneID	2540690.
GenomeReviews	Gene locus alp6 in contig CU329671_GR.
KEGG	spo:SPBC428.20c.
Organism-specific databases
GeneDB_Spombe	SPBC428.20c.
Phylogenomic databases
OrthoDB	EOG908PRV
Gene expression databases
ArrayExpress	Q9USQ2.
Family and domain databases
InterPro	IPR015697. GCP3. IPR007259. Spc97_Spc98. [Graphical view]
PANTHER	PTHR19302:SF14. GCP3. 1 hit. PTHR19302. Spc97_Spc98. 1 hit.
Pfam	PF04130. Spc97_Spc98. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALP6_SCHPO

Accession

Primary (citable) accession number: Q9USQ2
Secondary accession number(s): O94366, Q9P954

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	November 24, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)