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Q9USP8

- IDH2_SCHPO

UniProt

Q9USP8 - IDH2_SCHPO

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Protein

Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial

Gene

idh2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Performs an essential role in the oxidative function of the citric acid cycle and is involved in glutamate biosynthesis. Also binds RNA; specifically to the 5'-untranslated leaders of mitochondrial mRNAs.1 Publication

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291SubstrateBy similarity
Binding sitei139 – 1391SubstrateBy similarity
Binding sitei160 – 1601SubstrateBy similarity
Sitei167 – 1671Critical for catalysisBy similarity
Sitei214 – 2141Critical for catalysisBy similarity
Metal bindingi247 – 2471Magnesium or manganeseBy similarity
Binding sitei247 – 2471SubstrateBy similarity
Metal bindingi273 – 2731Magnesium or manganeseBy similarity
Metal bindingi277 – 2771Magnesium or manganeseBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NAD+) activity Source: PomBase
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamate biosynthetic process Source: PomBase
  2. isocitrate metabolic process Source: PomBase
  3. tricarboxylic acid cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_213505. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase
NAD(+)-specific ICDH
Gene namesi
Name:idh2
Synonyms:glu2
ORF Names:SPBC902.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC902.05c.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrial matrix Source: PomBase
  2. mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
BLAST
Chaini28 – 379352Isocitrate dehydrogenase [NAD] subunit 2, mitochondrialPRO_0000014433Add
BLAST

Proteomic databases

MaxQBiQ9USP8.
PaxDbiQ9USP8.
PRIDEiQ9USP8.

Interactioni

Subunit structurei

Octamer of two non-identical subunits IDH1 and IDH2.By similarity

Protein-protein interaction databases

BioGridi277774. 12 interactions.
MINTiMINT-4708417.
STRINGi4896.SPBC902.05c-1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
InParanoidiQ9USP8.
KOiK00030.
OrthoDBiEOG75XGWZ.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9USP8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMLSTLRTA GSLRTFSRSA CYSFQRFSST KAAAGTYEGV KNANGNYTVT
60 70 80 90 100
MIAGDGIGPE IAQSVERIFK AAKVPIEWER VKVYPILKNG TTTIPDDAKE
110 120 130 140 150
SVRKNKVALK GPLATPIGKG HVSMNLTLRR TFGLFANVRP CVSITGYKTP
160 170 180 190 200
YDNVNTVLIR ENTEGEYSGI EHEVIPGVVQ SIKLITRAAS ERVIRYAFQY
210 220 230 240 250
ARQTGKNNIT VVHKATIMRM ADGLFLECAK ELAPEYPDIE LREEILDNAC
260 270 280 290 300
LKIVTDPVPY NNTVMVMPNL YGDIVSDMCA GLIGGLGLTP SGNIGNQASI
310 320 330 340 350
FEAVHGTAPD IAGKGLANPT ALLLSSVMML KHMNLNDYAK RIESAIFDTL
360 370
ANNPDARTKD LGGKSNNVQY TDAIISKLK
Length:379
Mass (Da):41,188
Last modified:July 11, 2012 - v2
Checksum:iAE2FB477AC36D963
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB62099.2.
PIRiT50386.
RefSeqiNP_595203.2. NM_001021109.2.

Genome annotation databases

EnsemblFungiiSPBC902.05c.1; SPBC902.05c.1:pep; SPBC902.05c.
GeneIDi2541260.
KEGGispo:SPBC902.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB62099.2 .
PIRi T50386.
RefSeqi NP_595203.2. NM_001021109.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277774. 12 interactions.
MINTi MINT-4708417.
STRINGi 4896.SPBC902.05c-1.

Proteomic databases

MaxQBi Q9USP8.
PaxDbi Q9USP8.
PRIDEi Q9USP8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC902.05c.1 ; SPBC902.05c.1:pep ; SPBC902.05c .
GeneIDi 2541260.
KEGGi spo:SPBC902.05c.

Organism-specific databases

PomBasei SPBC902.05c.

Phylogenomic databases

eggNOGi COG0473.
HOGENOMi HOG000021113.
InParanoidi Q9USP8.
KOi K00030.
OrthoDBi EOG75XGWZ.

Enzyme and pathway databases

Reactomei REACT_213505. Citric acid cycle (TCA cycle).

Miscellaneous databases

NextBioi 20802372.
PROi Q9USP8.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Comparative functional genomics of the fission yeasts."
    Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
    , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
    Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  3. "Enzyme defects in glutamate-requiring strains of Schizosaccharomyces pombe."
    Barel I., MacDonald D.W.
    FEMS Microbiol. Lett. 113:267-272(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Isolation and RNA-binding analysis of NAD+ -isocitrate dehydrogenases from Kluyveromyces lactis and Schizosaccharomyces pombe."
    Elzinga S.D.J., van Oosterum K., Maat C., Grivell L.A., van der Spek H.
    Curr. Genet. 38:87-94(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIDH2_SCHPO
AccessioniPrimary (citable) accession number: Q9USP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 11, 2012
Last modified: November 26, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3