Reviewed, UniProtKB/Swiss-Prot Q9USP2 (YND1_SCHPO)

Names and origin

Protein names

Recommended name:
    Golgi apyrase
    EC=3.6.1.5
Alternative name(s):
    ATP-diphosphatase
    Adenosine diphosphatase
      Short name=ADPase
    ATP-diphosphohydrolase
    Golgi nucleoside diphosphatase

Gene names

Name:	ynd1
ORF Names:	SPCC11E10.05c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Required for Golgi glycosylation and cell wall integrity. Involved in N-mannosylation of proteins in Golgi. Ref.2 UniProtKB P40009
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate. Ref.2
Cofactor	Divalent metal cations. Ca2+, Mg2+ or Mn2+. Ref.2
Pathway	Protein modification; protein glycosylation. Ref.2
Subcellular location	Golgi apparatus. Membrane; Single-pass membrane protein Potential Ref.2 Ref.3.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid mannosylation Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	Golgi apparatus Ref.2 Ref.3 Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Ref.2 Inferred from genetic interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Golgi apyrase		PRO_0000347282
Regions
	Topological domain	1 – 470	470	Lumenal Potential UniProtKB P40009
	Transmembrane	471 – 491	21	Potential
	Topological domain	492 – 572	81	Cytoplasmic Potential UniProtKB P40009

Sequences

	Sequence		Length	Mass (Da)
	Q9USP2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8E039FBFA0D3B482	FASTA	572	64,680
	10 20 30 40 50 60 MVRKYGIFID AGSSGSRLLI YSWDYDTDSS LSDKVKKLPL IETGIGDGGK WSLKVQPGIS 70 80 90 100 110 120 SFANNPKHVG KKHLKELLDF AAHAIPKDVH KETPVFLSAT AGMRLLGVDA QNKILSHACR 130 140 150 160 170 180 YIKKNYDFDI PNCSNSIRVI DGKAEGMYGW LATNYLLKTL EEKDTSTVGF LDMGGASVQI 190 200 210 220 230 240 AFELPPSQLK NYKDSISTVH IGLQNGQQLE YPLFVTTWLG FGANEAYRRY LGLLIESENG 250 260 270 280 290 300 KVGNTLSDPC SLRGRTYDID GIEFAGTGDL KQCLKLTYNL LNKDKPCSMD PCNFDGISIP 310 320 330 340 350 360 PVDFANTEFV GVSEFWYTTN DVFDMGGSYH FPNFYKKVDE YCGTEWETML SRLYNKELTP 370 380 390 400 410 420 STDENKLEKL CFKASWALNV LHEGFDVPKS NTSSNDAKDG LSVIPAYHSP FTSLEKIERT 430 440 450 460 470 480 EVSWTLGQVL LYASNQQLLA KPEYANYYMD PYGKLIASPS KHWMRLFPNK LFFILSFIFC 490 500 510 520 530 540 LFFLFSLVLF GYDPKRRQRF KKFLLRLQRR KAPYIMSANG SYEDIADFSD DLEMSSPSKW 550 560 570 HGPPIRTTSS HVLADRLSFT ASRERTPRSP FP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Nucleoside diphosphatase and glycosyltransferase activities can localize to different subcellular compartments in Schizosaccharomyces pombe." D'Alessio C., Trombetta E.S., Parodi A.J. J. Biol. Chem. 278:22379-22387(2003) [PubMed: 12686557] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, COFACTOR, SUBCELLULAR LOCATION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAB57847.1.
PIR	T40856.
RefSeq	NP_588201.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9USP2.
Genome annotation databases
GeneID	2538741.
GenomeReviews	Gene locus ynd1 in contig CU329672_GR.
KEGG	spo:SPCC11E10.05c.
NMPDR	fig|4896.1.peg.539.
Organism-specific databases
GeneDB_Spombe	SPCC11E10.05c.
Phylogenomic databases
eggNOG	fuNOG04417.
OMA	QIAFELP.
OrthoDB	EOG9Q5B5R.
PhylomeDB	Q9USP2.
Gene expression databases
ArrayExpress	Q9USP2.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YND1_SCHPO

Accession

Primary (citable) accession number: Q9USP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)