Q9USP2 (YND1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi apyrase
EC=3.6.1.5

Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
Golgi nucleoside diphosphatase

Gene names

Name:	ynd1
ORF Names:	SPCC11E10.05c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Required for Golgi glycosylation and cell wall integrity. Involved in N-mannosylation of proteins in Golgi. Ref.2 UniProtKB P40009
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate. Ref.2
Cofactor	Divalent metal cations. Ca2+, Mg2+ or Mn2+. Ref.2
Pathway	Protein modification; protein glycosylation. Ref.2
Subcellular location	Golgi apparatus. Membrane; Single-pass membrane protein Potential Ref.2 Ref.3.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein mannosylation Inferred from mutant phenotype Ref.2. Source: PomBase
Cellular_component	Golgi apparatus Inferred from direct assay Ref.2Ref.3. Source: PomBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-diphosphatase activity Inferred from genetic interaction Ref.2. Source: PomBase nucleoside-triphosphate diphosphatase activity Inferred from genetic interaction. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Golgi apyrase		PRO_0000347282
Regions
	Topological domain	1 – 470	470	Lumenal Potential UniProtKB P40009
	Transmembrane	471 – 491	21	Helical; Potential
	Topological domain	492 – 572	81	Cytoplasmic Potential UniProtKB P40009
Sites
	Active site	145	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9USP2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8E039FBFA0D3B482	FASTA	572	64,680
	10 20 30 40 50 60 MVRKYGIFID AGSSGSRLLI YSWDYDTDSS LSDKVKKLPL IETGIGDGGK WSLKVQPGIS 70 80 90 100 110 120 SFANNPKHVG KKHLKELLDF AAHAIPKDVH KETPVFLSAT AGMRLLGVDA QNKILSHACR 130 140 150 160 170 180 YIKKNYDFDI PNCSNSIRVI DGKAEGMYGW LATNYLLKTL EEKDTSTVGF LDMGGASVQI 190 200 210 220 230 240 AFELPPSQLK NYKDSISTVH IGLQNGQQLE YPLFVTTWLG FGANEAYRRY LGLLIESENG 250 260 270 280 290 300 KVGNTLSDPC SLRGRTYDID GIEFAGTGDL KQCLKLTYNL LNKDKPCSMD PCNFDGISIP 310 320 330 340 350 360 PVDFANTEFV GVSEFWYTTN DVFDMGGSYH FPNFYKKVDE YCGTEWETML SRLYNKELTP 370 380 390 400 410 420 STDENKLEKL CFKASWALNV LHEGFDVPKS NTSSNDAKDG LSVIPAYHSP FTSLEKIERT 430 440 450 460 470 480 EVSWTLGQVL LYASNQQLLA KPEYANYYMD PYGKLIASPS KHWMRLFPNK LFFILSFIFC 490 500 510 520 530 540 LFFLFSLVLF GYDPKRRQRF KKFLLRLQRR KAPYIMSANG SYEDIADFSD DLEMSSPSKW 550 560 570 HGPPIRTTSS HVLADRLSFT ASRERTPRSP FP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Nucleoside diphosphatase and glycosyltransferase activities can localize to different subcellular compartments in Schizosaccharomyces pombe." D'Alessio C., Trombetta E.S., Parodi A.J. J. Biol. Chem. 278:22379-22387(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY, COFACTOR, SUBCELLULAR LOCATION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAB57847.1.
PIR	T40856.
RefSeq	NP_588201.1. NM_001023191.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPCC11E10.05c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC11E10.05c.1; SPCC11E10.05c.1:pep; SPCC11E10.05c.
GeneID	2538741.
KEGG	spo:SPCC11E10.05c.
Organism-specific databases
PomBase	SPCC11E10.05c.
Phylogenomic databases
eggNOG	COG5371.
HOGENOM	HOG000246725.
OMA	PAPNIPL.
OrthoDB	EOG4WM83T.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. PTHR11782. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20799925.

Entry information

Entry name

YND1_SCHPO

Accession

Primary (citable) accession number: Q9USP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program