Q9USN5 (DHE2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative NAD-specific glutamate dehydrogenase
Short name=NAD-GDH
EC=1.4.1.2

Gene names

ORF Names:

SPCC132.04c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1106 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH.
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamate catabolic process to 2-oxoglutarate Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	glutamate dehydrogenase (NAD+) activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1106	1106	Putative NAD-specific glutamate dehydrogenase		PRO_0000310353
Sites
	Active site	654	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9USN5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5AFFEAB84F1295CA	FASTA	1,106	125,712
	10 20 30 40 50 60 MYLTKQIPHY SRIHTTQLLT VVNHRTIPFK IRGSFSNCRR YSNFATVPLL ERPFKNKSKH 70 80 90 100 110 120 NTARASRPFS TQKMLLTKSH PPNMYKDSVF FGYRPIVFSG KQDQKSKVIQ CLRTERKTIP 130 140 150 160 170 180 DDLVEDEVDR FYNKLGLDDY YFQMEPVSII ADHIEIIYAA KIAAHASHAK EELNIHVKNE 190 200 210 220 230 240 NEDLAIYLDS SPVTQPELDQ SSAVEESIST RYLDPFKLTD PTAYRVESFT SVTNIDRTST 250 260 270 280 290 300 ENSNIYTYFV TKCDFVDNPK KDASPDVPTD IASVSDKTFL EKASDNTIEM YQDVMNSVLT 310 320 330 340 350 360 RFGPVVRLFD YQGRSEIRLV VGYRRGSIFQ YFPSLSKLFR YYGLHSTRTY VEQFSNGVTI 370 380 390 400 410 420 ISYNFKPELF KNAAVTSINE LFSQITREAS LLYCLPSTDF QPLFVSEKLS IQEVTYAHCV 430 440 450 460 470 480 RIFCEHVMNK LGPEYSSLSA ILDHSNNIHA EILETIKRRL STLAFTRTKI HDTIMQYPGL 490 500 510 520 530 540 VHTLFEQFYL EHAINHNSTP HLHRAKSATS LADEASTYSI TPMSATALMD LIQKTCTNEE 550 560 570 580 590 600 DVSVMEMFVK FNTHLLKTNF FQTTKVALSF RFDPSFLDST QYKDPLYAMI MSIGNEFRGF 610 620 630 640 650 660 HLRFRDVARG GIRLIKSANP EAFGLNARGL FDENYNLAKT QMLKNKDIPE GGAKGVILLG 670 680 690 700 710 720 KDCQDKPELA FMKYIDSIID LLIVNKSQPL VDKLGKPEIL FMGPDENTAD LVNWATIHAH 730 740 750 760 770 780 RRNAPWWKSF FTGKKPTMGG IPHDKYGMTS LSVRCYVEGI YKKLNITDPS KLTKVQTGGP 790 800 810 820 830 840 DGDLGSNEIK LSNEKYIAVI DGSGVLYDPA GLDRTELLRL ADERKTIDHF DAGKLSPEGY 850 860 870 880 890 900 RVLVKDTNLK LPNGEIVRNG TIFRNTAHLR YKADTFVPCG GRPNAININN VEQLIDDHGR 910 920 930 940 950 960 PAFKYLVEGA NLFITQDAKS VLEKAGVIVI RDASANKGGV TSSSLEVLAS LSFDDASFKE 970 980 990 1000 1010 1020 NMCVHDGKVP TFYADYVNEV KRIIQRNANL EFEAIWKGHS ENKIPYTSLS NHLSTEIVKL 1030 1040 1050 1060 1070 1080 DHDIYNYEKL WADVGFRNAV LRASIPKTLQ AKIGLEKMLE RIPESYLRAI FSTYLASRFV 1090 1100 YQHVVSSDPF AFFDYISTEM KMLKDA

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329672 Genomic DNA. Translation: CAB58131.1.
PIR	T40931.
RefSeq	NP_588149.1. NM_001023138.1.
3D structure databases
ProteinModelPortal	Q9USN5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9USN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC132.04c.1; SPCC132.04c.1:pep; SPCC132.04c.
GeneID	2539148.
KEGG	spo:SPCC132.04c.
NMPDR	fig|4896.1.peg.487.
Organism-specific databases
GeneDB_Spombe	SPCC132.04c.
Phylogenomic databases
eggNOG	fuNOG04119.
GeneTree	EFGT00050000004146.
HOGENOM	HBG330596.
OMA	PCGGRPN.
OrthoDB	EOG47DDQ7.
Gene expression databases
ArrayExpress	Q9USN5.
Family and domain databases
InterPro	IPR006096. Glu/Leu/Phe/Val_DH_C. IPR016210. Glu_DH_NAD-dep. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K15371.
Pfam	PF00208. ELFV_dehydrog. 1 hit. [Graphical view]
PIRSF	PIRSF000184. GDH_NAD. 1 hit.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE2_SCHPO

Accession

Primary (citable) accession number: Q9USN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program