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Q9USL3

- SYIM_SCHPO

UniProt

Q9USL3 - SYIM_SCHPO

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Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

ism1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: PomBase

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: PomBase
  2. mitochondrial translation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ism1
ORF Names:SPCC18B5.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome III

Organism-specific databases

PomBaseiSPCC18B5.08c.

Subcellular locationi

Cytoplasm 1 Publication. Mitochondrion matrix By similarity

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 973973Isoleucine--tRNA ligase, mitochondrialPRO_0000339654Add
BLAST

Proteomic databases

MaxQBiQ9USL3.

Interactioni

Protein-protein interaction databases

MINTiMINT-4708152.
STRINGi4896.SPCC18B5.08c-1.

Structurei

3D structure databases

ProteinModelPortaliQ9USL3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi87 – 9711"HIGH" regionBy similarityAdd
BLAST
Motifi625 – 6295"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
InParanoidiQ9USL3.
KOiK01870.
OMAiEYPEGHI.
OrthoDBiEOG7JMGNT.
PhylomeDBiQ9USL3.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9USL3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFSYISSLP KGHNHICCKQ AGRFLSSQAD LKKYSESLCL PKTSFPIKPN
60 70 80 90 100
VKGNNEKYFK SITSDLYEWQ KENLNKEDSF VLLDGPPFAN GRLHIGHALN
110 120 130 140 150
KILKDIINRW QLLKGRSVHY VPGWDCHGLP IESKAIKANA ERKSSLEIRK
160 170 180 190 200
IAKDFANSAV QEQLMMFQRM AVMGDWPSRY ITMSDKFEIA ELKVFLSLLQ
210 220 230 240 250
KDLIFRQNKP VYWSSSSRSA LAESEIEYDD NHVSTSIYFT FPVNSFSIDG
260 270 280 290 300
CEYNNVKALV WTTTPWTIPS NLALSYHPEI NYGLYQHNNS IYLMSDNLVP
310 320 330 340 350
NLDFMQGAKR LASCPSDIIS SFTYENPLLP KQSFPFLQSN YVTNDIGTGI
360 370 380 390 400
VHVAPGHGME DYLLGLENNL RPFSPLDDYG RYTKEALDGS LEGLEVLGDG
410 420 430 440 450
GKKVISIMKN QNMIVKVSPY KHRYPYDWRT HKPLILRATP QWFISLENER
460 470 480 490 500
KTAIKALDSV KMIPPNSRAR LLGFLNGRPE WCISRQRAWG LPIPVLYEKG
510 520 530 540 550
TKIPLLTVKS VSYIIEKMEV EGVDSWFNDT ENNGHAQWVH PDYRNKEYIR
560 570 580 590 600
GTETLDVWFD SGTSWTTIAP RKNKPLIDLC LEGSDQHRGW FQSLLLTYTA
610 620 630 640 650
YQSKPEAPFS TLFTHGFIFD ERGQKQSKSL GNVTDPEDVI NGKLLKGKKL
660 670 680 690 700
PYGVDLLRLW VASCDSTNDT NLGPNILTQV GESLKKWRLT SRFCLGNLHD
710 720 730 740 750
WNTSSSVDVG ELRGIDKLAL VQLDKFQTEI RELYESYSIN KVVHHLNYFM
760 770 780 790 800
NSFLSSTYFD AVKDRLYADL PNSVSRRSVQ TVLYHSLLTL IWAISPITPL
810 820 830 840 850
LAQEIWQSLP DSYLNSSYQT PFHAGETHLI SSLRSKVDLL DRKFLIKEYL
860 870 880 890 900
VLQQLKYSIN LLITAARENL TIKNSLEAYV VIKSKSQSLL SFLKNYSSDL
910 920 930 940 950
PFLFNTSKVF INEIPENLKL ASVTQPEVQL DYGVANISLF FSNQQKCLRC
960 970
WMHTAHEDGL CDRCESVLAQ LKR
Length:973
Mass (Da):111,255
Last modified:May 1, 2000 - v1
Checksum:i7061018D18A6C43B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAB52155.1.
PIRiT41201.
RefSeqiNP_587938.1. NM_001022929.2.

Genome annotation databases

EnsemblFungiiSPCC18B5.08c.1; SPCC18B5.08c.1:pep; SPCC18B5.08c.
GeneIDi2539323.
KEGGispo:SPCC18B5.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329672 Genomic DNA. Translation: CAB52155.1 .
PIRi T41201.
RefSeqi NP_587938.1. NM_001022929.2.

3D structure databases

ProteinModelPortali Q9USL3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4708152.
STRINGi 4896.SPCC18B5.08c-1.

Proteomic databases

MaxQBi Q9USL3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPCC18B5.08c.1 ; SPCC18B5.08c.1:pep ; SPCC18B5.08c .
GeneIDi 2539323.
KEGGi spo:SPCC18B5.08c.

Organism-specific databases

PomBasei SPCC18B5.08c.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
InParanoidi Q9USL3.
KOi K01870.
OMAi EYPEGHI.
OrthoDBi EOG7JMGNT.
PhylomeDBi Q9USL3.

Miscellaneous databases

NextBioi 20800488.
PROi Q9USL3.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYIM_SCHPO
AccessioniPrimary (citable) accession number: Q9USL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3