Reviewed, UniProtKB/Swiss-Prot Q9USL3 (SYIM_SCHPO)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase, mitochondrial
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ism1
ORF Names:	SPCC18B5.08c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	973 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm. Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 973	973	Isoleucyl-tRNA synthetase, mitochondrial		PRO_0000339654
Regions
	Motif	87 – 97	11	"HIGH" region By similarity
	Motif	625 – 629	5	"KMSKS" region By similarity
Sites
	Binding site	628	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9USL3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7061018D18A6C43B	FASTA	973	111,255
	10 20 30 40 50 60 MKFSYISSLP KGHNHICCKQ AGRFLSSQAD LKKYSESLCL PKTSFPIKPN VKGNNEKYFK 70 80 90 100 110 120 SITSDLYEWQ KENLNKEDSF VLLDGPPFAN GRLHIGHALN KILKDIINRW QLLKGRSVHY 130 140 150 160 170 180 VPGWDCHGLP IESKAIKANA ERKSSLEIRK IAKDFANSAV QEQLMMFQRM AVMGDWPSRY 190 200 210 220 230 240 ITMSDKFEIA ELKVFLSLLQ KDLIFRQNKP VYWSSSSRSA LAESEIEYDD NHVSTSIYFT 250 260 270 280 290 300 FPVNSFSIDG CEYNNVKALV WTTTPWTIPS NLALSYHPEI NYGLYQHNNS IYLMSDNLVP 310 320 330 340 350 360 NLDFMQGAKR LASCPSDIIS SFTYENPLLP KQSFPFLQSN YVTNDIGTGI VHVAPGHGME 370 380 390 400 410 420 DYLLGLENNL RPFSPLDDYG RYTKEALDGS LEGLEVLGDG GKKVISIMKN QNMIVKVSPY 430 440 450 460 470 480 KHRYPYDWRT HKPLILRATP QWFISLENER KTAIKALDSV KMIPPNSRAR LLGFLNGRPE 490 500 510 520 530 540 WCISRQRAWG LPIPVLYEKG TKIPLLTVKS VSYIIEKMEV EGVDSWFNDT ENNGHAQWVH 550 560 570 580 590 600 PDYRNKEYIR GTETLDVWFD SGTSWTTIAP RKNKPLIDLC LEGSDQHRGW FQSLLLTYTA 610 620 630 640 650 660 YQSKPEAPFS TLFTHGFIFD ERGQKQSKSL GNVTDPEDVI NGKLLKGKKL PYGVDLLRLW 670 680 690 700 710 720 VASCDSTNDT NLGPNILTQV GESLKKWRLT SRFCLGNLHD WNTSSSVDVG ELRGIDKLAL 730 740 750 760 770 780 VQLDKFQTEI RELYESYSIN KVVHHLNYFM NSFLSSTYFD AVKDRLYADL PNSVSRRSVQ 790 800 810 820 830 840 TVLYHSLLTL IWAISPITPL LAQEIWQSLP DSYLNSSYQT PFHAGETHLI SSLRSKVDLL 850 860 870 880 890 900 DRKFLIKEYL VLQQLKYSIN LLITAARENL TIKNSLEAYV VIKSKSQSLL SFLKNYSSDL 910 920 930 940 950 960 PFLFNTSKVF INEIPENLKL ASVTQPEVQL DYGVANISLF FSNQQKCLRC WMHTAHEDGL 970 CDRCESVLAQ LKR

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329672 Genomic DNA. Translation: CAB52155.1.
PIR	T41201.
RefSeq	NP_587938.1.
3D structure databases
HSSP	HSSP built from PDB template 1FFY based on UniProtKB P41972.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9USL3.
Genome annotation databases
GeneID	2539323.
GenomeReviews	Gene locus ism1 in contig CU329672_GR.
KEGG	spo:SPCC18B5.08c.
NMPDR	fig|4896.1.peg.276.
Organism-specific databases
GeneDB_Spombe	SPCC18B5.08c.
Phylogenomic databases
OMA	FPMRGNL.
Gene expression databases
ArrayExpress	Q9USL3.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIM_SCHPO

Accession

Primary (citable) accession number: Q9USL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)