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Q9USL3 (SYIM_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, mitochondrial

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ism1
ORF Names:SPCC18B5.08c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length973 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Subcellular location

Cytoplasm. Mitochondrion matrix By similarity Ref.2.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 973973Isoleucine--tRNA ligase, mitochondrial HAMAP-Rule MF_02002
PRO_0000339654

Regions

Motif87 – 9711"HIGH" region By similarity
Motif625 – 6295"KMSKS" region By similarity

Sites

Binding site6281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9USL3 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 7061018D18A6C43B

FASTA973111,255
        10         20         30         40         50         60 
MKFSYISSLP KGHNHICCKQ AGRFLSSQAD LKKYSESLCL PKTSFPIKPN VKGNNEKYFK 

        70         80         90        100        110        120 
SITSDLYEWQ KENLNKEDSF VLLDGPPFAN GRLHIGHALN KILKDIINRW QLLKGRSVHY 

       130        140        150        160        170        180 
VPGWDCHGLP IESKAIKANA ERKSSLEIRK IAKDFANSAV QEQLMMFQRM AVMGDWPSRY 

       190        200        210        220        230        240 
ITMSDKFEIA ELKVFLSLLQ KDLIFRQNKP VYWSSSSRSA LAESEIEYDD NHVSTSIYFT 

       250        260        270        280        290        300 
FPVNSFSIDG CEYNNVKALV WTTTPWTIPS NLALSYHPEI NYGLYQHNNS IYLMSDNLVP 

       310        320        330        340        350        360 
NLDFMQGAKR LASCPSDIIS SFTYENPLLP KQSFPFLQSN YVTNDIGTGI VHVAPGHGME 

       370        380        390        400        410        420 
DYLLGLENNL RPFSPLDDYG RYTKEALDGS LEGLEVLGDG GKKVISIMKN QNMIVKVSPY 

       430        440        450        460        470        480 
KHRYPYDWRT HKPLILRATP QWFISLENER KTAIKALDSV KMIPPNSRAR LLGFLNGRPE 

       490        500        510        520        530        540 
WCISRQRAWG LPIPVLYEKG TKIPLLTVKS VSYIIEKMEV EGVDSWFNDT ENNGHAQWVH 

       550        560        570        580        590        600 
PDYRNKEYIR GTETLDVWFD SGTSWTTIAP RKNKPLIDLC LEGSDQHRGW FQSLLLTYTA 

       610        620        630        640        650        660 
YQSKPEAPFS TLFTHGFIFD ERGQKQSKSL GNVTDPEDVI NGKLLKGKKL PYGVDLLRLW 

       670        680        690        700        710        720 
VASCDSTNDT NLGPNILTQV GESLKKWRLT SRFCLGNLHD WNTSSSVDVG ELRGIDKLAL 

       730        740        750        760        770        780 
VQLDKFQTEI RELYESYSIN KVVHHLNYFM NSFLSSTYFD AVKDRLYADL PNSVSRRSVQ 

       790        800        810        820        830        840 
TVLYHSLLTL IWAISPITPL LAQEIWQSLP DSYLNSSYQT PFHAGETHLI SSLRSKVDLL 

       850        860        870        880        890        900 
DRKFLIKEYL VLQQLKYSIN LLITAARENL TIKNSLEAYV VIKSKSQSLL SFLKNYSSDL 

       910        920        930        940        950        960 
PFLFNTSKVF INEIPENLKL ASVTQPEVQL DYGVANISLF FSNQQKCLRC WMHTAHEDGL 

       970 
CDRCESVLAQ LKR 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329672 Genomic DNA. Translation: CAB52155.1.
PIRT41201.
RefSeqNP_587938.1. NM_001022929.2.

3D structure databases

ProteinModelPortalQ9USL3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4708152.
STRING4896.SPCC18B5.08c-1.

Proteomic databases

MaxQBQ9USL3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPCC18B5.08c.1; SPCC18B5.08c.1:pep; SPCC18B5.08c.
GeneID2539323.
KEGGspo:SPCC18B5.08c.

Organism-specific databases

PomBaseSPCC18B5.08c.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAEYPEGHI.
OrthoDBEOG7JMGNT.
PhylomeDBQ9USL3.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20800488.
PROQ9USL3.

Entry information

Entry nameSYIM_SCHPO
AccessionPrimary (citable) accession number: Q9USL3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries