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Q9USL3

- SYIM_SCHPO

UniProt

Q9USL3 - SYIM_SCHPO

Protein

Isoleucine--tRNA ligase, mitochondrial

Gene

ism1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei628 – 6281ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: PomBase

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: PomBase
    2. mitochondrial translation Source: PomBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ism1
    ORF Names:SPCC18B5.08c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome III

    Organism-specific databases

    PomBaseiSPCC18B5.08c.

    Subcellular locationi

    Cytoplasm 1 Publication. Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 973973Isoleucine--tRNA ligase, mitochondrialPRO_0000339654Add
    BLAST

    Proteomic databases

    MaxQBiQ9USL3.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4708152.
    STRINGi4896.SPCC18B5.08c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9USL3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi87 – 9711"HIGH" regionBy similarityAdd
    BLAST
    Motifi625 – 6295"KMSKS" regionBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEYPEGHI.
    OrthoDBiEOG7JMGNT.
    PhylomeDBiQ9USL3.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9USL3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFSYISSLP KGHNHICCKQ AGRFLSSQAD LKKYSESLCL PKTSFPIKPN    50
    VKGNNEKYFK SITSDLYEWQ KENLNKEDSF VLLDGPPFAN GRLHIGHALN 100
    KILKDIINRW QLLKGRSVHY VPGWDCHGLP IESKAIKANA ERKSSLEIRK 150
    IAKDFANSAV QEQLMMFQRM AVMGDWPSRY ITMSDKFEIA ELKVFLSLLQ 200
    KDLIFRQNKP VYWSSSSRSA LAESEIEYDD NHVSTSIYFT FPVNSFSIDG 250
    CEYNNVKALV WTTTPWTIPS NLALSYHPEI NYGLYQHNNS IYLMSDNLVP 300
    NLDFMQGAKR LASCPSDIIS SFTYENPLLP KQSFPFLQSN YVTNDIGTGI 350
    VHVAPGHGME DYLLGLENNL RPFSPLDDYG RYTKEALDGS LEGLEVLGDG 400
    GKKVISIMKN QNMIVKVSPY KHRYPYDWRT HKPLILRATP QWFISLENER 450
    KTAIKALDSV KMIPPNSRAR LLGFLNGRPE WCISRQRAWG LPIPVLYEKG 500
    TKIPLLTVKS VSYIIEKMEV EGVDSWFNDT ENNGHAQWVH PDYRNKEYIR 550
    GTETLDVWFD SGTSWTTIAP RKNKPLIDLC LEGSDQHRGW FQSLLLTYTA 600
    YQSKPEAPFS TLFTHGFIFD ERGQKQSKSL GNVTDPEDVI NGKLLKGKKL 650
    PYGVDLLRLW VASCDSTNDT NLGPNILTQV GESLKKWRLT SRFCLGNLHD 700
    WNTSSSVDVG ELRGIDKLAL VQLDKFQTEI RELYESYSIN KVVHHLNYFM 750
    NSFLSSTYFD AVKDRLYADL PNSVSRRSVQ TVLYHSLLTL IWAISPITPL 800
    LAQEIWQSLP DSYLNSSYQT PFHAGETHLI SSLRSKVDLL DRKFLIKEYL 850
    VLQQLKYSIN LLITAARENL TIKNSLEAYV VIKSKSQSLL SFLKNYSSDL 900
    PFLFNTSKVF INEIPENLKL ASVTQPEVQL DYGVANISLF FSNQQKCLRC 950
    WMHTAHEDGL CDRCESVLAQ LKR 973
    Length:973
    Mass (Da):111,255
    Last modified:May 1, 2000 - v1
    Checksum:i7061018D18A6C43B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAB52155.1.
    PIRiT41201.
    RefSeqiNP_587938.1. NM_001022929.2.

    Genome annotation databases

    EnsemblFungiiSPCC18B5.08c.1; SPCC18B5.08c.1:pep; SPCC18B5.08c.
    GeneIDi2539323.
    KEGGispo:SPCC18B5.08c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329672 Genomic DNA. Translation: CAB52155.1 .
    PIRi T41201.
    RefSeqi NP_587938.1. NM_001022929.2.

    3D structure databases

    ProteinModelPortali Q9USL3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4708152.
    STRINGi 4896.SPCC18B5.08c-1.

    Proteomic databases

    MaxQBi Q9USL3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPCC18B5.08c.1 ; SPCC18B5.08c.1:pep ; SPCC18B5.08c .
    GeneIDi 2539323.
    KEGGi spo:SPCC18B5.08c.

    Organism-specific databases

    PomBasei SPCC18B5.08c.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EYPEGHI.
    OrthoDBi EOG7JMGNT.
    PhylomeDBi Q9USL3.

    Miscellaneous databases

    NextBioi 20800488.
    PROi Q9USL3.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYIM_SCHPO
    AccessioniPrimary (citable) accession number: Q9USL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3