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Protein

Cell wall alpha-1,3-glucan synthase ags1

Gene

ags1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for alpha-1,3-glucan and alpha-1,4-glucan production which are required for cell wall synthesis.1 Publication

Catalytic activityi

UDP-glucose + (alpha-D-glucosyl-(1->3))(n) = UDP + (alpha-D-glucosyl-(1->3))(n+1).

GO - Molecular functioni

  • alpha-1,3-glucan synthase activity Source: PomBase
  • alpha-1,4-glucan synthase activity Source: PomBase

GO - Biological processi

  • alpha-glucan biosynthetic process Source: PomBase
  • cell wall organization Source: UniProtKB-KW
  • fungal-type cell wall (1->3)-alpha-glucan biosynthetic process Source: GO_Central
  • fungal-type cell wall biogenesis Source: PomBase
  • primary cell septum biogenesis Source: PomBase

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BRENDAi2.4.1.B3. 5613.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
GT5. Glycosyltransferase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall alpha-1,3-glucan synthase ags1 (EC:2.4.1.183)
Alternative name(s):
Cell wall alpha-1,4-glucan synthase
Gene namesi
Name:ags1
Synonyms:mok1
ORF Names:SPCC1281.01, SPCC17A7.01, SPCC338.01c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

PomBaseiSPCC1281.01. ags1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1526E → A: No accumulation of alpha-1,4-glucan. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803281 – 2410Cell wall alpha-1,3-glucan synthase ags1Add BLAST2410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1643Phosphoserine1 Publication1
Modified residuei1644Phosphoserine1 Publication1
Modified residuei1651Phosphoserine1 Publication1
Modified residuei1653Phosphothreonine1 Publication1
Modified residuei1738Phosphoserine1 Publication1
Modified residuei1812Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9USK8.
PRIDEiQ9USK8.

PTM databases

iPTMnetiQ9USK8.

Interactioni

Subunit structurei

Interacts with sad1.1 Publication

Protein-protein interaction databases

IntActiQ9USK8. 1 interactor.
STRINGi4896.SPCC1281.01.1.

Structurei

3D structure databases

ProteinModelPortaliQ9USK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family.Curated

Phylogenomic databases

HOGENOMiHOG000163916.
InParanoidiQ9USK8.
OrthoDBiEOG092C010V.
PhylomeDBiQ9USK8.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR001296. Glyco_trans_1.
IPR017853. Glycoside_hydrolase_SF.
IPR020846. MFS_dom.
IPR013534. Starch_synth_cat_dom.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9USK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHGLQGLCFR RAVIALALLL FHSVFAAPYS EDEEPWNLNQ NKNASSVLEY
60 70 80 90 100
SGEWADHDFF PSPDNWRMSF ITVILDRWYD GDPSNNDIEK TPFEYDISEV
110 120 130 140 150
SFRNGGDIVG LELSLDYLEG LGTQGIYIAG TPFVNMPWGA DQYSPLDYTI
160 170 180 190 200
LDHHLGTIDQ WRSTITAMHE RGMYLVVDLT VATLGDLIGM VGHLNDTSGV
210 220 230 240 250
DFNLNEYNAM WKTSEYRYVD FNFTNVYNTS CEYPRFWGED GGPVSIQFKG
260 270 280 290 300
CYVSDFDHYG DTEAFGSHPD WQRQLSKFAS VQDRLRDWKP DVAEKLMHLS
310 320 330 340 350
CLVISMLDVD GFRIDKATQM TADFIVDWSM YVRECAAKYN KKNFLIVGEV
360 370 380 390 400
TGSSSYGSIY YNRGRQPDQR PPNVTAAFNY TSDESQYSLR DSDHYGFDGS
410 420 430 440 450
AFHYSIYRAL LRFLGMDSKM EIDFDVSSVL TTAWNGIQIN EDAVNINTGT
460 470 480 490 500
VEPLHMYGVA NHDVFRWGAI ENGTARLILG TMITSLLFPG IPLLYYGDEQ
510 520 530 540 550
GMYVLDNSAN NYLYGRQAMN SARAWYIHGC YNGSATSYPT VDLSPAQRGC
560 570 580 590 600
QDSWNYLDHF DIASAHRNVY RNIHSIRRHY LSLSEGWRFD HIANWTDDVY
610 620 630 640 650
FPDSQPYASP MGLYSVLRGP MKEIQDFDSI TNASNVSKSE VWVLYANRND
660 670 680 690 700
THLWSYDCTD EDSAIIGPWK SGTTLRNLIY PYDTIELEDS WNSSWGCIPN
710 720 730 740 750
IELDPYAFKL YVPEEDFIEN DPIITSLTPE HDARVVASGN EIDLTIEFSR
760 770 780 790 800
SMDCDSIKNA LSVVSSTRPK NTTAVIDVDS SFCRNYSEDA STSLHGQTAG
810 820 830 840 850
RFAWYGTLTN IDPGIHRISL KSVPTSDFSS RTLSTDNFLI RVGSTNNPIV
860 870 880 890 900
HYSANYSDTL LIMQDGDLYI NHSAPGAVLF RYSTDFQSHW SDWEEYNGGL
910 920 930 940 950
TKVQASNWTG TRRQGWEGHH IHVQYWSDLG GSANHMQQSD YGFKYRRFLP
960 970 980 990 1000
HMFLEGDFNE YGYDSGVENR FLQKSDFYWE AGFISETYPA AIQLNVWGMN
1010 1020 1030 1040 1050
PDGIPDKTRV YGSQGNSTVL SRSDPASLVG NNITIYHPPP HGYLSYKILL
1060 1070 1080 1090 1100
RDDDMIYRLA PSGEWGVSIA IYVLCIVIPP LSAIVVSWAF KNSFYTVKFN
1110 1120 1130 1140 1150
KHGNNDLGKF YPLKSLVPFR KKNDLDSPAK VTPVVSGVSA RKKKCVLIAT
1160 1170 1180 1190 1200
LEYDITDWKI RIKIGGLGVM AQLMAQHLKH EDLVWVVPCV GDVVYPEAEE
1210 1220 1230 1240 1250
ASPIEVKIID QTYTINVYHH YLDNIKYVLL DAPVFRRQTS KEPYPARMDD
1260 1270 1280 1290 1300
LGSAIFYSAW NQCIAEVIRR NPIDIYHIND YHGALAPCYL LPDIIPCALS
1310 1320 1330 1340 1350
LHNAEFQGLW PLRTPEEKEE VCAVYNISQR VCTKYVQFGN VFNLLHAAVS
1360 1370 1380 1390 1400
YIRIHQKGFG AVGVSNKYGK RSWARYPIFW GLKKIGKLPN PDPTDTDEIV
1410 1420 1430 1440 1450
DDKAVAITDI DPDMEKSKVE HKRLAQEWAG LEVNEKYDLL VFVGRWSSQK
1460 1470 1480 1490 1500
GIDLIADIAP SLLESYKVQL ICVGPIIDLY GKFAAEKLDV LQKKYPTRVF
1510 1520 1530 1540 1550
SQPKFTQLPP YIFSGADFAL IPSRDEPFGL VAVEFGRKGA LGIGARVGGL
1560 1570 1580 1590 1600
GQMPGWWYSV ESSATPHLLK QFEQACQQAL SSSQRTRARL RARSAKQRFP
1610 1620 1630 1640 1650
VSQWKAKLEA LTDGCIKCSQ KYGRNSRSRS SFYSLIHESF SRSSEVLPTS
1660 1670 1680 1690 1700
SDTNLDAKRA EEAEMIMIET PPTAEANTGA KLDRSLSLGS RRGPGHTTED
1710 1720 1730 1740 1750
DASDGLDTIQ EESMTAGDST SGGSDISRYR AERLNPDSHS PSEYSFDSGD
1760 1770 1780 1790 1800
YEFDPQRSYY YDDLFDDDTT IRNAPSFRPQ MGSFDAEHAV GATFSQDDLS
1810 1820 1830 1840 1850
DPARSVDSDS VSPPLPPFVA GSNPNARNNN NPYFYGNLHT ESSLSLASVM
1860 1870 1880 1890 1900
SGKEKRDFSL TRVEETFTDE DGQALRSFSE KLQKLNAKNS KDDLCIEQYL
1910 1920 1930 1940 1950
MKSERSFFHE RRAIKLGLQK PNKLHVNELS SHSGTEESES LSNGQTSYDD
1960 1970 1980 1990 2000
IIAMTDESNY TQLGDDDFKT IHGLKKFMLF KIYDWPIYSI FLALGQILAA
2010 2020 2030 2040 2050
TAYQLTLFTG TSNIQTYEIY SVCAFFIGAS FVWWFMFARL PSYYVLSIPW
2060 2070 2080 2090 2100
LFYAVALFLV GLPAFDTVAP GRVWITNVAA WIYAIASASG SIFFSLNFGE
2110 2120 2130 2140 2150
EGAVQTRIWV FRACLVQGVQ QVWSAALWYW GAHLNKRLTA GEANTFKMSP
2160 2170 2180 2190 2200
AIPSITWPLS AVSILIFALL FKGLPEYYRQ LSGSIPAFYK SLLRRKLVVW
2210 2220 2230 2240 2250
FCISVFLQNF WLSSLNGRSW SYLWDIGNIH QWQIFLLIVA FYIVLWALLL
2260 2270 2280 2290 2300
GVLAWISRTH SWIICVFGVG LGAPRWLQQF WATSNIGLYL PWAGYSGPYL
2310 2320 2330 2340 2350
GRTLWLWLGV LDAIQSVGIG MILLQTLTRR HVASTLMTGQ IVGAVATMIG
2360 2370 2380 2390 2400
RGASPNREGP ANVFIDFTKW NHGDGSSILA SAPFWINIIC QLAICVGYLA
2410
FFRRENLSRP
Length:2,410
Mass (Da):272,106
Last modified:February 12, 2003 - v3
Checksum:i3C0959CDAEA1092C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256F → Y in CAA19332 (PubMed:11859360).Curated1
Sequence conflicti1849V → A in BAA34054 (PubMed:10087262).Curated1
Sequence conflicti2028G → C in BAA34054 (PubMed:10087262).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019183 Genomic DNA. Translation: BAA34054.1.
AF061180 Genomic DNA. Translation: AAC31430.1.
AF063305 mRNA. Translation: AAC39519.1.
CU329672 Genomic DNA. Translation: CAA19332.1.
D89202 mRNA. Translation: BAA13863.1.
PIRiT43731.

Similar proteinsi

Entry informationi

Entry nameiAGS1_SCHPO
AccessioniPrimary (citable) accession number: Q9USK8
Secondary accession number(s): P78852, Q9URK2, Q9URT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 12, 2003
Last modified: July 5, 2017
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families