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Protein

Mediator of RNA polymerase II transcription subunit 6

Gene

med6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 6
Alternative name(s):
Mediator complex subunit 6
RNA polymerase II mediator complex protein pmc5
Gene namesi
Name:med6
Synonyms:pmc5
ORF Names:SPAC1002.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1002.15c.
PomBaseiSPAC1002.15c. med6.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: GO_Central
  • mediator complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Mediator of RNA polymerase II transcription subunit 6PRO_0000096390Add
BLAST

Proteomic databases

MaxQBiQ9US45.

Interactioni

Subunit structurei

Component of the Mediator complex.2 Publications

Protein-protein interaction databases

BioGridi279691. 107 interactions.
DIPiDIP-38748N.
IntActiQ9US45. 1 interaction.

Structurei

Secondary structure

1
216
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 133Combined sources
Helixi19 – 246Combined sources
Turni30 – 323Combined sources
Helixi33 – 386Combined sources
Helixi48 – 5811Combined sources
Helixi66 – 716Combined sources
Beta strandi75 – 8410Combined sources
Turni85 – 873Combined sources
Beta strandi88 – 969Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi102 – 11110Combined sources
Beta strandi114 – 1174Combined sources
Helixi120 – 14021Combined sources
Beta strandi148 – 1503Combined sources
Turni151 – 1533Combined sources
Beta strandi154 – 1563Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H61X-ray2.70A/B10-180[»]
4H63X-ray3.40F1-180[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 6 family.Curated

Phylogenomic databases

HOGENOMiHOG000093949.
InParanoidiQ9US45.
KOiK15128.
OMAiGNVYQAP.
OrthoDBiEOG7MD52F.
PhylomeDBiQ9US45.

Family and domain databases

InterProiIPR007018. Mediator_Med6.
[Graphical view]
PANTHERiPTHR13104. PTHR13104. 1 hit.
PfamiPF04934. Med6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9US45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE
60 70 80 90 100
MLKMQSQFNA LDLGDLNSQL KRLTGIQFVI IHERPPFLWV IQKQNRLNEN
110 120 130 140 150
EVKPLTVYFV CNENIYMAPN AYTLLATRML NATYCFQKAL TKIEKFPQYN
160 170 180 190 200
PQEGYTYPKL SNDNLEVDHS NTNEPADENK NQSIENADYS FSPEDFSVVR
210
AFMQSLHSSK EAPDVK
Length:216
Mass (Da):24,906
Last modified:May 1, 2000 - v1
Checksum:i84AE0C11F607F35A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB65615.1.
RefSeqiNP_593503.1. NM_001018937.2.

Genome annotation databases

EnsemblFungiiSPAC1002.15c.1; SPAC1002.15c.1:pep; SPAC1002.15c.
GeneIDi2543263.
KEGGispo:SPAC1002.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB65615.1.
RefSeqiNP_593503.1. NM_001018937.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H61X-ray2.70A/B10-180[»]
4H63X-ray3.40F1-180[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279691. 107 interactions.
DIPiDIP-38748N.
IntActiQ9US45. 1 interaction.

Proteomic databases

MaxQBiQ9US45.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1002.15c.1; SPAC1002.15c.1:pep; SPAC1002.15c.
GeneIDi2543263.
KEGGispo:SPAC1002.15c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1002.15c.
PomBaseiSPAC1002.15c. med6.

Phylogenomic databases

HOGENOMiHOG000093949.
InParanoidiQ9US45.
KOiK15128.
OMAiGNVYQAP.
OrthoDBiEOG7MD52F.
PhylomeDBiQ9US45.

Miscellaneous databases

PROiQ9US45.

Family and domain databases

InterProiIPR007018. Mediator_Med6.
[Graphical view]
PANTHERiPTHR13104. PTHR13104. 1 hit.
PfamiPF04934. Med6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe."
    Spaehr H., Beve J., Larsson T., Bergstroem J., Karlsson K.-A., Gustafsson C.M.
    J. Biol. Chem. 275:1351-1356(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    Strain: 972 / ATCC 24843.
  3. "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells."
    Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D., Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.
    Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.

Entry informationi

Entry nameiMED6_SCHPO
AccessioniPrimary (citable) accession number: Q9US45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.