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Protein

Putative uracil phosphoribosyltransferase urg2

Gene

urg2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.Curated

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP.By similarity

Enzyme regulationi

Allosterically activated by GTP.Curated

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uracil.
Proteins known to be involved in this subpathway in this organism are:
  1. Uracil phosphoribosyltransferase 1 (SPAC1B3.01c), Uracil phosphoribosyltransferase 2 (SPAC1399.04c), Putative uracil phosphoribosyltransferase urg2 (urg2)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uracil, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 7515-phospho-alpha-D-ribose 1-diphosphateBy similarity
Binding sitei100 – 10015-phospho-alpha-D-ribose 1-diphosphateBy similarity
Binding sitei187 – 1871Ribose-5-phosphateBy similarity
Binding sitei188 – 1881Uracil; via amide nitrogenBy similarity
Binding sitei194 – 19415-phospho-alpha-D-ribose 1-diphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative uracil phosphoribosyltransferase urg2 (EC:2.4.2.9)
Short name:
UPRTase urg2
Alternative name(s):
UMP pyrophosphorylase urg2
Gene namesi
Name:urg2Imported
ORF Names:SPAC1002.17c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1002.17c.
PomBaseiSPAC1002.17c. urg2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Putative uracil phosphoribosyltransferase urg2PRO_0000309558Add
BLAST

Proteomic databases

MaxQBiQ9US43.

Interactioni

Protein-protein interaction databases

BioGridi279688. 17 interactions.
MINTiMINT-4707677.

Structurei

3D structure databases

ProteinModelPortaliQ9US43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 13495-phospho-alpha-D-ribose 1-diphosphate bindingBy similarity
Regioni193 – 1953Uracil bindingBy similarity

Sequence similaritiesi

Belongs to the UPRTase family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000262754.
InParanoidiQ9US43.
KOiK00761.
OMAiIQTLREW.
OrthoDBiEOG7NW6NF.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
InterProiIPR029057. PRTase-like.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9US43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTTVSAI RTVEEMSNIT ISSHPVVNEQ IAILRDRSLK PSQVRSVVDE
60 70 80 90 100
ISRFLAYEST KTLKSPSVSI VPVLRSGMSM MSAFSKVLPD VPIYHIGIFR
110 120 130 140 150
EKSTLQPIEY YNKLPKKSTD TAVILDPVMA TGGTANAVIT TLQEWGCKNI
160 170 180 190 200
IFVSVLASEQ ALTRFSNIPG VEFVIGAVDK SLDAKGYLVP GVGDIGDRLY

GATA
Length:204
Mass (Da):22,084
Last modified:June 13, 2012 - v2
Checksum:i4F52E9EFB6BDAF8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB65617.2.
RefSeqiNP_593505.2. NM_001018939.2.

Genome annotation databases

EnsemblFungiiSPAC1002.17c.1; SPAC1002.17c.1:pep; SPAC1002.17c.
GeneIDi2543260.
KEGGispo:SPAC1002.17c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB65617.2.
RefSeqiNP_593505.2. NM_001018939.2.

3D structure databases

ProteinModelPortaliQ9US43.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279688. 17 interactions.
MINTiMINT-4707677.

Proteomic databases

MaxQBiQ9US43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1002.17c.1; SPAC1002.17c.1:pep; SPAC1002.17c.
GeneIDi2543260.
KEGGispo:SPAC1002.17c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1002.17c.
PomBaseiSPAC1002.17c. urg2.

Phylogenomic databases

HOGENOMiHOG000262754.
InParanoidiQ9US43.
KOiK00761.
OMAiIQTLREW.
OrthoDBiEOG7NW6NF.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00636.

Miscellaneous databases

PROiQ9US43.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
InterProiIPR029057. PRTase-like.
[Graphical view]
PfamiPF14681. UPRTase. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Comparative functional genomics of the fission yeasts."
    Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
    , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
    Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUPP3_SCHPO
AccessioniPrimary (citable) accession number: Q9US43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 13, 2012
Last modified: June 8, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.