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Protein

Alpha-galactosidase mel1

Gene

mel1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Secreted alpha-galactosidase required for catabolic conversion of melibiose to glucose and galactose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541NucleophileBy similarity
Active sitei214 – 2141Proton donorBy similarity

GO - Molecular functioni

  • alpha-galactosidase activity Source: PomBase
  • raffinose alpha-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-SPO-1660662. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.
mycoCLAPiMEL27A_SCHPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase mel1 (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase
Gene namesi
Name:mel1
ORF Names:SPAC869.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC869.07c.
PomBaseiSPAC869.07c. mel1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • endoplasmic reticulum Source: PomBase
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • extracellular region Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 436412Alpha-galactosidase mel1PRO_0000001006Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 77By similarity
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence analysis
Disulfide bondi126 ↔ 156By similarity
Glycosylationi180 – 1801N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9URZ0.

Interactioni

Protein-protein interaction databases

MINTiMINT-4707322.

Structurei

3D structure databases

ProteinModelPortaliQ9URZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000161224.
InParanoidiQ9URZ0.
KOiK07407.
OMAiMTCARYA.
OrthoDBiEOG7B8SD5.
PhylomeDBiQ9URZ0.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR006215. Glyco_hydro_melibiase.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9URZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISISFLNCF FLVFLFLFFS DVHGSYNGLG LKPQMGWNSW NKYACDIDES
60 70 80 90 100
IILNNAKAIK EEGLLDLGYE YIVMDDCWSK HERNATTGRL EANPDKFPNG
110 120 130 140 150
IGSMAKKLHD MGFKFGMYSS AGKYTCAGFP GSLNHEQIDA DTFADWGVDY
160 170 180 190 200
LKYDNCFNEG KSGVPLISYE RYKRMSDALN KTGRPIFYSL CQWGEDFVWN
210 220 230 240 250
WGNTIANSWR ISGDIFDTFS RKDVRCPCET IECFALQGDH CSVMNIISKA
260 270 280 290 300
SFLSSKAGMN SGWNDLDSLE VGNGGMSFEE YKTHFTMWAI LKSPLILGND
310 320 330 340 350
VSSMSPMDKL IVSNKELISI NQDIGTNPAA LIWKKKYGDE YIELFSGRLS
360 370 380 390 400
NNDWVVAVLN AASEPLKMGI HLSDIFVDAL GNAEHDWLAT DLWNNNVKLV
410 420 430
SDRIRANVAS HGVQVWRFQQ YKVKNTNDKF FSFNKH
Length:436
Mass (Da):49,366
Last modified:May 1, 2000 - v1
Checksum:i8B3EE243AE39BD50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB60017.1.
PIRiT39118.
RefSeqiNP_595012.1. NM_001020443.2.

Genome annotation databases

EnsemblFungiiSPAC869.07c.1; SPAC869.07c.1:pep; SPAC869.07c.
GeneIDi2543243.
KEGGispo:SPAC869.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB60017.1.
PIRiT39118.
RefSeqiNP_595012.1. NM_001020443.2.

3D structure databases

ProteinModelPortaliQ9URZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4707322.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.
mycoCLAPiMEL27A_SCHPO.

Proteomic databases

MaxQBiQ9URZ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC869.07c.1; SPAC869.07c.1:pep; SPAC869.07c.
GeneIDi2543243.
KEGGispo:SPAC869.07c.

Organism-specific databases

EuPathDBiFungiDB:SPAC869.07c.
PomBaseiSPAC869.07c. mel1.

Phylogenomic databases

HOGENOMiHOG000161224.
InParanoidiQ9URZ0.
KOiK07407.
OMAiMTCARYA.
OrthoDBiEOG7B8SD5.
PhylomeDBiQ9URZ0.

Enzyme and pathway databases

ReactomeiR-SPO-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

PROiQ9URZ0.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR006215. Glyco_hydro_melibiase.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Development of a semi-quantitative plate-based alpha-galactosidase gene reporter for Schizosaccharomyces pombe and its use to isolate a constitutively active Mam2."
    Goddard A., Ladds G., Davey J.
    Yeast 22:31-41(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAGAL_SCHPO
AccessioniPrimary (citable) accession number: Q9URZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.