Q9URZ0 (AGAL_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable alpha-galactosidase
EC=3.2.1.22

Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase

Gene names

ORF Names:

SPAC869.07c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	extracellular polysaccharide metabolic process Inferred by curator. Source: GeneDB_Spombe glycoside catabolic process Inferred from sequence or structural similarity. Source: RefGenome glycosylceramide catabolic process Inferred from sequence or structural similarity. Source: RefGenome melibiose catabolic process Inferred by curator. Source: GeneDB_Spombe
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: GeneDB_Spombe external side of plasma membrane Traceable author statement. Source: GeneDB_Spombe extracellular region Inferred by curator. Source: GeneDB_Spombe
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 436	412	Probable alpha-galactosidase		PRO_0000001006
Sites
	Active site	154	1	Nucleophile By similarity
	Active site	214	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 77		By similarity
	Disulfide bond	126 ↔ 156		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9URZ0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8B3EE243AE39BD50	FASTA	436	49,366
	10 20 30 40 50 60 MISISFLNCF FLVFLFLFFS DVHGSYNGLG LKPQMGWNSW NKYACDIDES IILNNAKAIK 70 80 90 100 110 120 EEGLLDLGYE YIVMDDCWSK HERNATTGRL EANPDKFPNG IGSMAKKLHD MGFKFGMYSS 130 140 150 160 170 180 AGKYTCAGFP GSLNHEQIDA DTFADWGVDY LKYDNCFNEG KSGVPLISYE RYKRMSDALN 190 200 210 220 230 240 KTGRPIFYSL CQWGEDFVWN WGNTIANSWR ISGDIFDTFS RKDVRCPCET IECFALQGDH 250 260 270 280 290 300 CSVMNIISKA SFLSSKAGMN SGWNDLDSLE VGNGGMSFEE YKTHFTMWAI LKSPLILGND 310 320 330 340 350 360 VSSMSPMDKL IVSNKELISI NQDIGTNPAA LIWKKKYGDE YIELFSGRLS NNDWVVAVLN 370 380 390 400 410 420 AASEPLKMGI HLSDIFVDAL GNAEHDWLAT DLWNNNVKLV SDRIRANVAS HGVQVWRFQQ 430 YKVKNTNDKF FSFNKH

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB60017.1.
PIR	T39118.
RefSeq	NP_595012.1. NM_001020443.1.
3D structure databases
ProteinModelPortal	Q9URZ0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9URZ0.
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC869.07c.1; SPAC869.07c.1:pep; SPAC869.07c.
GeneID	2543243.
KEGG	spo:SPAC869.07c.
NMPDR	fig|4896.1.peg.4982.
Organism-specific databases
GeneDB_Spombe	SPAC869.07c.
Phylogenomic databases
eggNOG	fuNOG07713.
GeneTree	EFGT00050000001473.
HOGENOM	HBG424315.
OMA	MTRALMK.
OrthoDB	EOG4F4WKH.
Gene expression databases
ArrayExpress	Q9URZ0.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
KO	K07407.
Pfam	PF02065. Melibiase. 2 hits. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27. PR00748. MELIBIASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_SCHPO

Accession

Primary (citable) accession number: Q9URZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program