Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Flavohemoprotein

Gene

SPAC869.02c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O2 and NAD(P)H to convert NO to nitrate, which protects the fungus from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress (By similarity).By similarity
In the presence of oxygen and NADH, it has NADH oxidase activity, which leads to the generation of superoxide and H2O2. Under anaerobic conditions, it also exhibits nitric oxide reductase and FAD reductase activities. However, all these reactions are much lower than NOD activity (By similarity).By similarity

Catalytic activityi

2 nitric oxide + 2 O2 + NAD(P)H = 2 nitrate + NAD(P)+ + H+.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • heme bBy similarityNote: Binds 1 heme b group per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Involved in heme-bound ligand stabilization and O-O bond activationBy similarity1
Sitei113Influences the redox potential of the prosthetic heme and FAD groupsBy similarity1
Metal bindingi114Iron (heme proximal ligand)PROSITE-ProRule annotationBy similarity1
Active sitei124Charge relay systemBy similarity1
Active sitei167Charge relay systemBy similarity1
Binding sitei216FADBy similarity1
Sitei420Influences the redox potential of the prosthetic heme and FAD groupsBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi232 – 235FADBy similarity4
Nucleotide bindingi301 – 306NADPBy similarity6
Nucleotide bindingi421 – 424FADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processDetoxification
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Flavohemoprotein (EC:1.14.12.17)
Alternative name(s):
Flavohemoglobin
Hemoglobin-like protein
Nitric oxide dioxygenase
Short name:
NO oxygenase
Short name:
NOD
Gene namesi
ORF Names:SPAC869.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC869.02c
PomBaseiSPAC869.02c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002802191 – 427FlavohemoproteinAdd BLAST427

Proteomic databases

MaxQBiQ9URY5
PaxDbiQ9URY5
PRIDEiQ9URY5

Interactioni

Protein-protein interaction databases

BioGridi278580, 9 interactors
STRINGi4896.SPAC869.02c.1

Structurei

3D structure databases

ProteinModelPortaliQ9URY5
SMRiQ9URY5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 285FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 170GlobinSequence analysisAdd BLAST143
Regioni176 – 427ReductaseSequence analysisAdd BLAST252
Regioni290 – 427NAD or NADP-bindingSequence analysisAdd BLAST138

Domaini

Consists of two distinct domains; a N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.Curated

Sequence similaritiesi

Belongs to the globin family. Two-domain flavohemoproteins subfamily.PROSITE-ProRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Sequence analysis

Phylogenomic databases

HOGENOMiHOG000238921
InParanoidiQ9URY5
KOiK05916
OMAiIVHKHAA
OrthoDBiEOG092C2IP8
PhylomeDBiQ9URY5

Family and domain databases

Gene3Di1.10.490.10, 1 hit
InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR000971 Globin
IPR009050 Globin-like_sf
IPR012292 Globin/Proto
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00042 Globin, 1 hit
PF00175 NAD_binding_1, 1 hit
SUPFAMiSSF46458 SSF46458, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS01033 GLOBIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9URY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVEVNREN ADVANTNRQA NLAEGYEIKE LNESQKQYIR SSIPILESSG
60 70 80 90 100
VNLTKAFYQK MLGNYPEVLP YFNKAHQISL SQPRILAFAL LNYAKNIDDL
110 120 130 140 150
TSLSAFMDQI VVKHVGLQIK AEHYPIVGHC LLSTMQELLP SDVATPAFLE
160 170 180 190 200
AWTTAYGNLA KILIDSEKKV YQSQPWNGFV EFKVTELINE SSDVKSVYLG
210 220 230 240 250
PKDPAFRISH AHPGQYVSVL WEIPGLSHKT LREYSLSNRV DTCRNQFRIS
260 270 280 290 300
VRRVAGGVVS NFVHDNLKVG DIVGVSPPAG NFVYKRSEEN VNRPLLCFAG
310 320 330 340 350
GIGITPLIPI IETALLDGRK VNFCYSSRNY VSRPFKQWLE QLKLKYKENL
360 370 380 390 400
KLKEFFSEES SVTKEQIVDE VMTRIINEED LEKLDLSECD IYMLGPNNYM
410 420
RFVKQELVKL GVEPNKVQSE FFGPYIP
Length:427
Mass (Da):48,459
Last modified:May 1, 2000 - v1
Checksum:i869E5D4578F309D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB60012.1
PIRiT39113
RefSeqiNP_595017.1, NM_001020448.2

Genome annotation databases

EnsemblFungiiSPAC869.02c.1; SPAC869.02c.1:pep; SPAC869.02c
GeneIDi2542104
KEGGispo:SPAC869.02c

Entry informationi

Entry nameiFHP_SCHPO
AccessioniPrimary (citable) accession number: Q9URY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health