Microtubule protein alp7 - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

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Q9URY2 (ALP7_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 71. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Microtubule protein alp7

Alternative name(s):
Altered polarity protein 7
Transforming acidic coiled-coil protein mia1
Short name=TACC protein mia1

Gene names

Name:	alp7
Synonyms:	mia1
ORF Names:	SPAC890.02c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for bipolar spindle formation and proper chromosome segregation. Has an indirect role in connecting the kinetochores and the plus end of pole to chromosome microtubules by targeting alp14 to the spindle pole body. Involved in the emergence of large microtubule-organizing centers (MTOC) in interphase cells. Attaches to the minus ends of microtubules and associates with the sites of microtubule attachment on the nuclear envelope. This leads to the stabilzation of the microtubule bundles. Ref.3 Ref.4
Subunit structure	Interacts with alp14. Ref.3
Subcellular location	Nucleus. Cytoplasm. Cytoplasm › cytoskeleton › spindle. Chromosome › centromere › kinetochore. Note: Associated with the equatorial MTOC, spindle midzones, spindle pole body and mitotic kinetochore periphery. Spindle and kinetochore localization is alp14-dependent. Ref.2 Ref.3 Ref.4 Ref.5

Ontologies

Keywords
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Microtubule Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	astral microtubule organization Inferred from mutant phenotype. Source: GeneDB_Spombe cellular protein localization Inferred from genetic interaction. Source: GeneDB_Spombe establishment of mitotic spindle orientation Inferred from mutant phenotype. Source: GeneDB_Spombe spindle assembly involved in mitosis Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	TACC/TOG complex Inferred from direct assay. Source: GeneDB_Spombe condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: GeneDB_Spombe spindle pole body Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	protein binding Inferred from physical interaction Ref.3. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
alp14	O94534	5	EBI-1556697,EBI-1556727

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 474

474

Microtubule protein alp7

PRO_0000064570

Regions

Coiled coil

219 – 273

Potential

Coiled coil

367 – 471

105

Potential

Compositional bias

15 – 20

Poly-Ser

Amino acid modifications

Modified residue

Phosphoserine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

Q9URY2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 22B4216C196E310C
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FASTA

474

53,175

        10         20         30         40         50         60 
MSDIVSSSTD YSRRSPSSSS IGTNETDHTG FHEKRQGASS ESLIPPAQRS SEESMPAPKL 

        70         80         90        100        110        120 
FPKLTSKPNP QLNLKDTLNK RVSDRLQALE LNKSFDFSGT PRPMHPISHP LSQHKTPEFK 

       130        140        150        160        170        180 
HRKRNVESIL TPKNPSLFSS SNAASQRGSL NTAPSNFAYS HSSSLQTSAS SRPPVLSNGS 

       190        200        210        220        230        240 
FPRQTNTAPL NPPVHLKDNI RNSATPSTSQ ADIPTQYPIN STQKQQAKYE AEIEGYKAKL 

       250        260        270        280        290        300 
AGTYHEISVL QNTIVNVSGQ LIAVNDQLQQ LRSGKASTSP STKDTNMRLV EGHNEETLAL 

       310        320        330        340        350        360 
QRGKYTQEEV DKLIQERMEK VAEDLHAQYS AKHTQKINAF KANYARKYEA TIQELQNQIG 

       370        380        390        400        410        420 
TAPNAPKISN SNWEEERRAL KADNQTLQKQ LEKAIQERQD MSDFLNNFKA DMAKSDKLLM 

       430        440        450        460        470 
QQQSQQTGDL ETLRLQLQAL QEELRVEREE RQQLIQMSED LVIAMDQLNL EQKS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 104-273, SUBCELLULAR LOCATION. Strain: ATCC 38364 / 968.
[3]	"Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation." Sato M., Vardy L., Angel Garcia M., Koonrugsa N., Toda T. Mol. Biol. Cell 15:1609-1622(2004) [PubMed: 14742702] [Abstract] Cited for: FUNCTION, INTERACTION WITH ALP14, SUBCELLULAR LOCATION.
[4]	"The fission yeast transforming acidic coiled coil-related protein Mia1p/Alp7p is required for formation and maintenance of persistent microtubule-organizing centers at the nuclear envelope." Zheng L., Schwartz C., Wee L., Oliferenko S. Mol. Biol. Cell 17:2212-2222(2006) [PubMed: 16481403] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB63493.1. AB027802 Genomic DNA. Translation: BAA87106.1.
PIR	T50258.
RefSeq	NP_594820.1. NM_001020249.1.
3D structure databases
ProteinModelPortal	Q9URY2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9URY2. 4 interactions.
STRING	Q9URY2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC890.02c.1; SPAC890.02c.1:pep; SPAC890.02c.
GeneID	2543382.
GenomeReviews	Gene locus alp7 in contig CU329670_GR.
KEGG	spo:SPAC890.02c.
NMPDR	fig\|4896.1.peg.4790.
Organism-specific databases
GeneDB_Spombe	SPAC890.02c.
Phylogenomic databases
OrthoDB	EOG4FN7S5.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002893-MONOMER.
Gene expression databases
ArrayExpress	Q9URY2.
Family and domain databases
InterPro	IPR024312. Kinetocho_Slk19. IPR007707. TACC. [Graphical view]
Pfam	PF12709. Kinetocho_Slk19. 1 hit. PF05010. TACC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALP7_SCHPO

Accession

Primary (citable) accession number: Q9URY2
Secondary accession number(s): Q9UU56

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents