Reviewed, UniProtKB/Swiss-Prot Q9URS1 (DHE4_PENCH)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:

GDH

Organism

Penicillium chrysogenum (Penicillium notatum)

Taxonomic identifier

5076 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	NADP-specific glutamate dehydrogenase		PRO_0000182793
Sites
	Active site	115	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9URS1-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 76A014A29150B4DE	FASTA	461	49,832
	10 20 30 40 50 60 MMQNLPFEPE FEQAYKELAS TLENSTLFQK KPEYRKALQV VSVPERVIQF RVVWEDDKGQ 70 80 90 100 110 120 VQINRGYRVQ FNSALGPYKG GLRFHPTVNL SILKFLGFEQ IFKNALTGLN MGGGKGGSDF 130 140 150 160 170 180 DPKGKTDNEI RRFCVSFMTE LCKHIGADTD VPAGDIGVTG REVGFMFGQY KKIRNQWEGV 190 200 210 220 230 240 LTGKGGSWGG SLIRPEATGY GVVYYVEHMI QHASGGKESF AGKRVAISGS GNVAQYAALK 250 260 270 280 290 300 VIELGGSVIS LSDSQGALVL NGEEGSFTAE EINTIAEIKV QRKQIAELAT QDAFSSKFKY 310 320 330 340 350 360 IPGARPWTNI AGRIDVALPS ATQNEVSGDE AKALIAAGCK FIAEGSNMGS TQEAIDVFEA 370 380 390 400 410 420 HRDANPGAAA IWYAPGKAAN AGGVAVSGLE MAQNSARVNW SREEVDSRLK KIMEDCFNNG 430 440 450 460 LSTAKEYVTP AEGVLPSLVA GSNIAGFTKV AEAMKEHGDW W

References

[1]

"The NADP-dependent glutamate dehydrogenase gene from Penicillium chrysogenum and the construction of expression vectors for filamentous fungi." Diez B., Mellado E., Rodriguez M., Bernasconi E., Barredo J.L. Appl. Microbiol. Biotechnol. 52:196-207(1999) [PubMed: 10499259] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF056974 Genomic DNA. Translation: AAF00006.1.
3D structure databases
SMR	Q9URS1. Positions 3-460.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 285.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_PENCH

Accession

Primary (citable) accession number: Q9URS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)