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Q9URQ5

- HTL1_YEAST

UniProt

Q9URQ5 - HTL1_YEAST

Protein

High temperature lethal protein 1

Gene

HTL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Required for cell cycle progression through G2/M transition at temperatures higher than 33 degrees Celsius. Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. When associated with the RSC complex, may act coordinately with PKC1 to regulate G2/M transition. Together with LDB7, NPL6, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.3 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. chromatin remodeling Source: SGD
    3. G2/M transition of mitotic cell cycle Source: UniProtKB
    4. nucleosome disassembly Source: SGD
    5. regulation of cell cycle Source: SGD
    6. regulation of transcription, DNA-templated Source: UniProtKB-KW
    7. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29401-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High temperature lethal protein 1
    Alternative name(s):
    Chromatin structure-remodeling complex protein HTL1
    Gene namesi
    Name:HTL1
    Synonyms:DRT1
    Ordered Locus Names:YCR020W-B
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR020w-b.
    SGDiS000006439. HTL1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. RSC complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 7877High temperature lethal protein 1PRO_0000084085Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9URQ5.
    PaxDbiQ9URQ5.
    PeptideAtlasiQ9URQ5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9URQ5.

    Interactioni

    Subunit structurei

    Interacts directly with RSC8. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSC8P436093EBI-8717,EBI-23005
    STH1P325973EBI-8717,EBI-18410

    Protein-protein interaction databases

    BioGridi31003. 55 interactions.
    IntActiQ9URQ5. 21 interactions.
    MINTiMINT-2779715.
    STRINGi4932.YCR020W-B.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9URQ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG257626.
    HOGENOMiHOG000000899.
    KOiK11769.
    OrthoDBiEOG7MPRTD.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9URQ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQNNTISSM NPERAYNNVT LKNLTAFQLL SQRENICELL NLVESTERHN   50
    SIINPERQRM SLEEMKKMLD ALKNERKK 78
    Length:78
    Mass (Da):9,180
    Last modified:May 1, 2000 - v1
    Checksum:i62308D5A1F6322C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF162267 Genomic DNA. Translation: AAD47275.1.
    X59720 Genomic DNA. Translation: CAC42974.1.
    AY558371 Genomic DNA. Translation: AAS56697.1.
    BK006937 Genomic DNA. Translation: DAA07499.1.
    PIRiS07696.
    RefSeqiNP_009949.1. NM_001184313.1.

    Genome annotation databases

    EnsemblFungiiYCR020W-B; YCR020W-B; YCR020W-B.
    GeneIDi850384.
    KEGGisce:YCR020W-B.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF162267 Genomic DNA. Translation: AAD47275.1 .
    X59720 Genomic DNA. Translation: CAC42974.1 .
    AY558371 Genomic DNA. Translation: AAS56697.1 .
    BK006937 Genomic DNA. Translation: DAA07499.1 .
    PIRi S07696.
    RefSeqi NP_009949.1. NM_001184313.1.

    3D structure databases

    ProteinModelPortali Q9URQ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31003. 55 interactions.
    IntActi Q9URQ5. 21 interactions.
    MINTi MINT-2779715.
    STRINGi 4932.YCR020W-B.

    Proteomic databases

    MaxQBi Q9URQ5.
    PaxDbi Q9URQ5.
    PeptideAtlasi Q9URQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR020W-B ; YCR020W-B ; YCR020W-B .
    GeneIDi 850384.
    KEGGi sce:YCR020W-B.

    Organism-specific databases

    CYGDi YCR020w-b.
    SGDi S000006439. HTL1.

    Phylogenomic databases

    eggNOGi NOG257626.
    HOGENOMi HOG000000899.
    KOi K11769.
    OrthoDBi EOG7MPRTD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29401-MONOMER.

    Miscellaneous databases

    NextBioi 965894.

    Gene expression databases

    Genevestigatori Q9URQ5.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The DRT1 gene encodes a novel small ORF necessary for viability at 36 degrees Celsius."
      Davidov E., Jiang W., Bailey D.A.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Dissecting the pet18 mutation in Saccharomyces cerevisiae: HTL1 encodes a 7-kDa polypeptide that interacts with components of the RSC complex."
      Lu Y.-M., Lin Y.-R., Tsai A., Hsao Y.-S., Li C.-C., Cheng M.Y.
      Mol. Genet. Genomics 269:321-330(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RSC8.
    3. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "HTL1 encodes a novel factor that interacts with the RSC chromatin remodeling complex in Saccharomyces cerevisiae."
      Romeo M.J., Angus-Hill M.L., Sobering A.K., Kamada Y., Cairns B.R., Levin D.E.
      Mol. Cell. Biol. 22:8165-8174(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE RSC COMPLEX.
    7. "The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37 degrees C, and for the conservation of chromosome stability and fertility."
      Lanzuolo C., Ederle S., Pollice A., Russo F., Storlazzi A., Pulitzer J.F.
      Yeast 18:1317-1330(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion."
      Baetz K.K., Krogan N.J., Emili A., Greenblatt J., Hieter P.
      Mol. Cell. Biol. 24:1232-1244(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RSC COMPLEX.
    10. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
      Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
      Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX.
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHTL1_YEAST
    AccessioniPrimary (citable) accession number: Q9URQ5
    Secondary accession number(s): D6VR30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3