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Q9URQ5

- HTL1_YEAST

UniProt

Q9URQ5 - HTL1_YEAST

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Protein

High temperature lethal protein 1

Gene
HTL1, DRT1, YCR020W-B
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for cell cycle progression through G2/M transition at temperatures higher than 33 degrees Celsius. Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. When associated with the RSC complex, may act coordinately with PKC1 to regulate G2/M transition. Together with LDB7, NPL6, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.3 Publications

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. chromatin remodeling Source: SGD
  3. G2/M transition of mitotic cell cycle Source: UniProtKB
  4. nucleosome disassembly Source: SGD
  5. regulation of cell cycle Source: SGD
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29401-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
High temperature lethal protein 1
Alternative name(s):
Chromatin structure-remodeling complex protein HTL1
Gene namesi
Name:HTL1
Synonyms:DRT1
Ordered Locus Names:YCR020W-B
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR020w-b.
SGDiS000006439. HTL1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. RSC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 7877High temperature lethal protein 1PRO_0000084085Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9URQ5.
PaxDbiQ9URQ5.
PeptideAtlasiQ9URQ5.

Expressioni

Gene expression databases

GenevestigatoriQ9URQ5.

Interactioni

Subunit structurei

Interacts directly with RSC8. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of a fungal-specific module (HTL1-LDB7-NPL6-RSC3-RSC30) within the RSC complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RSC8P436093EBI-8717,EBI-23005
STH1P325973EBI-8717,EBI-18410

Protein-protein interaction databases

BioGridi31003. 55 interactions.
IntActiQ9URQ5. 21 interactions.
MINTiMINT-2779715.
STRINGi4932.YCR020W-B.

Structurei

3D structure databases

ProteinModelPortaliQ9URQ5.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG257626.
HOGENOMiHOG000000899.
KOiK11769.
OrthoDBiEOG7MPRTD.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9URQ5-1 [UniParc]FASTAAdd to Basket

« Hide

MSQNNTISSM NPERAYNNVT LKNLTAFQLL SQRENICELL NLVESTERHN   50
SIINPERQRM SLEEMKKMLD ALKNERKK 78
Length:78
Mass (Da):9,180
Last modified:May 1, 2000 - v1
Checksum:i62308D5A1F6322C7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF162267 Genomic DNA. Translation: AAD47275.1.
X59720 Genomic DNA. Translation: CAC42974.1.
AY558371 Genomic DNA. Translation: AAS56697.1.
BK006937 Genomic DNA. Translation: DAA07499.1.
PIRiS07696.
RefSeqiNP_009949.1. NM_001184313.1.

Genome annotation databases

EnsemblFungiiYCR020W-B; YCR020W-B; YCR020W-B.
GeneIDi850384.
KEGGisce:YCR020W-B.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF162267 Genomic DNA. Translation: AAD47275.1 .
X59720 Genomic DNA. Translation: CAC42974.1 .
AY558371 Genomic DNA. Translation: AAS56697.1 .
BK006937 Genomic DNA. Translation: DAA07499.1 .
PIRi S07696.
RefSeqi NP_009949.1. NM_001184313.1.

3D structure databases

ProteinModelPortali Q9URQ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31003. 55 interactions.
IntActi Q9URQ5. 21 interactions.
MINTi MINT-2779715.
STRINGi 4932.YCR020W-B.

Proteomic databases

MaxQBi Q9URQ5.
PaxDbi Q9URQ5.
PeptideAtlasi Q9URQ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR020W-B ; YCR020W-B ; YCR020W-B .
GeneIDi 850384.
KEGGi sce:YCR020W-B.

Organism-specific databases

CYGDi YCR020w-b.
SGDi S000006439. HTL1.

Phylogenomic databases

eggNOGi NOG257626.
HOGENOMi HOG000000899.
KOi K11769.
OrthoDBi EOG7MPRTD.

Enzyme and pathway databases

BioCyci YEAST:G3O-29401-MONOMER.

Miscellaneous databases

NextBioi 965894.

Gene expression databases

Genevestigatori Q9URQ5.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DRT1 gene encodes a novel small ORF necessary for viability at 36 degrees Celsius."
    Davidov E., Jiang W., Bailey D.A.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Dissecting the pet18 mutation in Saccharomyces cerevisiae: HTL1 encodes a 7-kDa polypeptide that interacts with components of the RSC complex."
    Lu Y.-M., Lin Y.-R., Tsai A., Hsao Y.-S., Li C.-C., Cheng M.Y.
    Mol. Genet. Genomics 269:321-330(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RSC8.
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "HTL1 encodes a novel factor that interacts with the RSC chromatin remodeling complex in Saccharomyces cerevisiae."
    Romeo M.J., Angus-Hill M.L., Sobering A.K., Kamada Y., Cairns B.R., Levin D.E.
    Mol. Cell. Biol. 22:8165-8174(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE RSC COMPLEX.
  7. "The HTL1 gene (YCR020W-b) of Saccharomyces cerevisiae is necessary for growth at 37 degrees C, and for the conservation of chromosome stability and fertility."
    Lanzuolo C., Ederle S., Pollice A., Russo F., Storlazzi A., Pulitzer J.F.
    Yeast 18:1317-1330(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion."
    Baetz K.K., Krogan N.J., Emili A., Greenblatt J., Hieter P.
    Mol. Cell. Biol. 24:1232-1244(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RSC COMPLEX.
  10. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
    Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
    Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RSC COMPLEX.
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHTL1_YEAST
AccessioniPrimary (citable) accession number: Q9URQ5
Secondary accession number(s): D6VR30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3550 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

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