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Protein

tRNA-specific adenosine deaminase subunit TAD3

Gene

TAD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deaminates adenosine-34 to inosine in many tRNAs.

GO - Molecular functioni

  • tRNA-specific adenosine deaminase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • tRNA modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciYEAST:YLR316C-MONOMER.
BRENDAi3.5.4.33. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase subunit TAD3
Alternative name(s):
tRNA-specific adenosine-34 deaminase subunit TAD3
Gene namesi
Name:TAD3
Ordered Locus Names:YLR316C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR316C.
SGDiS000004308. TAD3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322tRNA-specific adenosine deaminase subunit TAD3PRO_0000171741Add
BLAST

Proteomic databases

MaxQBiQ9URQ3.
PeptideAtlasiQ9URQ3.

Interactioni

Subunit structurei

Heterodimer with TAD2.

Binary interactionsi

WithEntry#Exp.IntActNotes
TAD2P470582EBI-2094330,EBI-18939

Protein-protein interaction databases

BioGridi31581. 50 interactions.
DIPiDIP-5917N.
IntActiQ9URQ3. 1 interaction.
MINTiMINT-4495868.

Structurei

3D structure databases

ProteinModelPortaliQ9URQ3.
SMRiQ9URQ3. Positions 167-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 283122CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000010706.
HOGENOMiHOG000111185.
InParanoidiQ9URQ3.
KOiK15442.
OMAiNPNDQIL.
OrthoDBiEOG74J9HW.

Family and domain databases

InterProiIPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9URQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKKVNNPLK IDYQNGIIEN RLLQIRNFKD VNTPKLINVW SIRIDPRDSK
60 70 80 90 100
KVIELIRNDF QKNDPVSLRH LKRIRKDIET STLEVVLCSK EYICDEGEIN
110 120 130 140 150
NKLKSIWVGT KKYELSDDIE VPEFAPSTKE LNNAWSVKYW PLIWNGNPND
160 170 180 190 200
QILNDYKIDM QEVRNELSRA STLSVKMATA GKQFPMVSVF VDPSRKKDKV
210 220 230 240 250
VAEDGRNCEN SLPIDHSVMV GIRAVGERLR EGVDEDANSY LCLDYDVYLT
260 270 280 290 300
HEPCSMCSMA LIHSRVRRVV FLTEMQRTGS LKLTSGDGYC MNDNKQLNST
310 320
YEAFQWIGEE YPVGQVDRDV CC
Length:322
Mass (Da):37,107
Last modified:May 1, 2000 - v1
Checksum:i08D0878F10B36403
GO

Sequence cautioni

The sequence AAB64529.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242668 Genomic DNA. Translation: CAB60630.1.
U20618 Genomic DNA. Translation: AAB64529.1. Different initiation.
BK006945 Genomic DNA. Translation: DAA09626.1.
RefSeqiNP_013420.2. NM_001182205.1.

Genome annotation databases

EnsemblFungiiYLR316C; YLR316C; YLR316C.
GeneIDi851027.
KEGGisce:YLR316C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242668 Genomic DNA. Translation: CAB60630.1.
U20618 Genomic DNA. Translation: AAB64529.1. Different initiation.
BK006945 Genomic DNA. Translation: DAA09626.1.
RefSeqiNP_013420.2. NM_001182205.1.

3D structure databases

ProteinModelPortaliQ9URQ3.
SMRiQ9URQ3. Positions 167-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31581. 50 interactions.
DIPiDIP-5917N.
IntActiQ9URQ3. 1 interaction.
MINTiMINT-4495868.

Proteomic databases

MaxQBiQ9URQ3.
PeptideAtlasiQ9URQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR316C; YLR316C; YLR316C.
GeneIDi851027.
KEGGisce:YLR316C.

Organism-specific databases

EuPathDBiFungiDB:YLR316C.
SGDiS000004308. TAD3.

Phylogenomic databases

GeneTreeiENSGT00390000010706.
HOGENOMiHOG000111185.
InParanoidiQ9URQ3.
KOiK15442.
OMAiNPNDQIL.
OrthoDBiEOG74J9HW.

Enzyme and pathway databases

BioCyciYEAST:YLR316C-MONOMER.
BRENDAi3.5.4.33. 984.

Miscellaneous databases

PROiQ9URQ3.

Family and domain databases

InterProiIPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An adenosine deaminase that generates inosine at the wobble position of transfer RNAs."
    Gerber A.P., Keller W.
    Science 286:1146-1149(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: BMA41.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAD3_YEAST
AccessioniPrimary (citable) accession number: Q9URQ3
Secondary accession number(s): D6VYW0, O13552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 892 molecules/cell in log phase SD medium.1 Publication

Caution

In contrast to other cytidine and deoxycytidylate deaminase, lacks to conserved Glu active site in position 218 which is replaced by a Val residue, suggesting that it acts as a regulatory subunit.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.