Reviewed, UniProtKB/Swiss-Prot Q9UR35 (NCB5R_MORAP)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 1
    EC=1.6.2.2
Alternative name(s):
    Microsomal cytochrome b reductase

Gene names

Name:

CBR1

Organism

Mortierella alpina (Mortierella renispora)

Taxonomic identifier

64518 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mortierellales › Mortierellaceae › Mortierella

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	NADH-cytochrome b5 reductase 1		PRO_0000330158
Regions
	Transmembrane	14 – 34	21	Potential
	Domain	56 – 159	104	FAD-binding FR-type
	Nucleotide binding	139 – 154	16	FAD By similarity
	Nucleotide binding	165 – 197	33	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UR35-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 31764BE085A802C4	FASTA	298	32,331
	10 20 30 40 50 60 MTLSNPAIAA ASGVILAGAY LIDPSALPFV AAGVAATWAR VLFKKTAVKT PPMDPKEYRK 70 80 90 100 110 120 FKLVDKVHCS PNTAMYKFAL PHEDDLLNLP IGQHISIMAN INGKDISRSY TPTSSSDDVG 130 140 150 160 170 180 HFVLCIKSYP QGNISKMFSE LSIGDSINAR GPKGQFSYTP NMCRAIGMIA GGTGLTPMLQ 190 200 210 220 230 240 IIRAIVKNPE DKTQVNFIFA NVTEEDIILK AELDLLSQKH PQFKVYYVLN NAPEGWTGGV 250 260 270 280 290 GFVNADMIKE HMPAPAADIK VLLCGPPPMV SAMSKITQDL GYDKVNAVSK LPDQVFKF

References

[1]

"Identification of an NADH-cytochrome b(5) reductase gene from an arachidonic acid-producing fungus, Mortierella alpina 1S-4, by sequencing of the encoding cDNA and heterologous expression in a fungus, Aspergillus oryzae." Sakuradani E., Kobayashi M., Shimizu S. Appl. Environ. Microbiol. 65:3873-3879(1999) [PubMed: 10473389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 1S-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020034 mRNA. Translation: BAA85586.1. AB020035 Genomic DNA. Translation: BAA85587.1.
3D structure databases
HSSP	HSSP built from PDB template 1UMK based on UniProtKB P00387.
SMR	Q9UR35. Positions 49-298.
ModBase	Search...
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_MORAP

Accession

Primary (citable) accession number: Q9UR35

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)