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Protein

Aristolochene synthase

Gene

Ari1

Organism
Aspergillus terreus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic sesquiterpene aristolochene. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce aristolochene. Aristolochene is the likely parent compound for a number of sesquiterpenoid toxins produced by filamentous fungi.2 Publications

Catalytic activityi

(2E,6E)-farnesyl diphosphate = aristolochene + diphosphate.1 Publication

Cofactori

Mg2+Note: Binds 3 Mg2+ ions per monomer.

Kineticsi

  1. KM=13.5 nM for (2E,6E)-farnesyl diphosphate2 Publications
  1. Vmax=23.6 nmol/min/mg enzyme2 Publications

pH dependencei

Optimum pH is 8.0.2 Publications

Pathwayi: aristolochene biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes aristolochene from farnesyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Aristolochene synthase (Ari1)
This subpathway is part of the pathway aristolochene biosynthesis, which is itself part of Sesquiterpene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes aristolochene from farnesyl diphosphate, the pathway aristolochene biosynthesis and in Sesquiterpene biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90Magnesium 12 Publications1
Metal bindingi90Magnesium 22 Publications1
Binding sitei175Substrate1 Publication1
Metal bindingi219Magnesium 32 Publications1
Metal bindingi223Magnesium 32 Publications1
Binding sitei226Substrate1 Publication1
Metal bindingi227Magnesium 32 Publications1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.3.9. 536.
UniPathwayiUPA00177; UER00582.

Names & Taxonomyi

Protein namesi
Recommended name:
Aristolochene synthase (EC:4.2.3.9)
Short name:
AS
Alternative name(s):
Sesquiterpene cyclase
Gene namesi
Name:Ari1
OrganismiAspergillus terreus
Taxonomic identifieri33178 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi227E → Q: Abolishes catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004185501 – 320Aristolochene synthaseAdd BLAST320

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1320
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 43Combined sources15
Helixi49 – 58Combined sources10
Helixi60 – 67Combined sources8
Turni73 – 75Combined sources3
Helixi76 – 92Combined sources17
Helixi93 – 95Combined sources3
Helixi98 – 112Combined sources15
Helixi123 – 138Combined sources16
Helixi140 – 144Combined sources5
Helixi147 – 157Combined sources11
Helixi160 – 163Combined sources4
Helixi168 – 178Combined sources11
Helixi181 – 192Combined sources12
Helixi198 – 203Combined sources6
Helixi205 – 233Combined sources29
Turni236 – 239Combined sources4
Helixi244 – 252Combined sources9
Helixi256 – 283Combined sources28
Turni284 – 286Combined sources3
Helixi289 – 311Combined sources23
Helixi313 – 316Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E4OX-ray2.20A/B/C/D1-320[»]
2OA6X-ray2.15A/B/C/D1-320[»]
3BNXX-ray2.10A/B/C/D1-320[»]
3BNYX-ray1.89A/B/C/D1-320[»]
3CKEX-ray2.40A/B/C/D1-320[»]
4KUXX-ray1.90A/B/C/D14-320[»]
4KVDX-ray2.40A/B/C/D14-320[»]
4KVIX-ray2.15A/B/C/D14-320[»]
4KVWX-ray2.10A/B/C/D14-320[»]
4KVYX-ray1.95A/B/C/D14-320[»]
4KWDX-ray1.86A/B/C/D14-320[»]
5IMIX-ray2.46A/B/C/D14-320[»]
5IMNX-ray2.53A/B/C/D14-320[»]
5IMPX-ray2.04A/B/C/D14-320[»]
5IN8X-ray2.35A/B/C/D14-320[»]
5IVGX-ray1.95A/B/C/D14-320[»]
ProteinModelPortaliQ9UR08.
SMRiQ9UR08.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UR08.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni314 – 315Substrate-binding2

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UR08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPNGTNGA SSSLEPPPST FQPLCHPLVE EVSKEVDGYF LQHWNFPNEK
60 70 80 90 100
ARKKFVAAGF SRVTCLYFPK ALDDRIHFAC RLLTVLFLID DLLEYMSFEE
110 120 130 140 150
GSAYNEKLIP ISRGDVLPDR SIPVEYIIYD LWESMRAHDR EMADEILEPV
160 170 180 190 200
FLFMRAQTDR TRARPMGLGG YLEYRERDVG KELLAALMRF SMGLKLSPSE
210 220 230 240 250
LQRVREIDAN CSKHLSVVND IYSYEKELYT SKTAHSEGGI LCTSVQILAQ
260 270 280 290 300
EADVTAEAAK RVLFVMCREW ELRHQLLVAR LSAEGLETPG LAAYVEGLEY
310 320
QMSGNELWSQ TTLRYSVVVD
Length:320
Mass (Da):36,481
Last modified:May 1, 2000 - v1
Checksum:i73190707711ADC2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198359 mRNA. Translation: AAF13263.1.
AF198360 Genomic DNA. Translation: AAF13264.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198359 mRNA. Translation: AAF13263.1.
AF198360 Genomic DNA. Translation: AAF13264.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E4OX-ray2.20A/B/C/D1-320[»]
2OA6X-ray2.15A/B/C/D1-320[»]
3BNXX-ray2.10A/B/C/D1-320[»]
3BNYX-ray1.89A/B/C/D1-320[»]
3CKEX-ray2.40A/B/C/D1-320[»]
4KUXX-ray1.90A/B/C/D14-320[»]
4KVDX-ray2.40A/B/C/D14-320[»]
4KVIX-ray2.15A/B/C/D14-320[»]
4KVWX-ray2.10A/B/C/D14-320[»]
4KVYX-ray1.95A/B/C/D14-320[»]
4KWDX-ray1.86A/B/C/D14-320[»]
5IMIX-ray2.46A/B/C/D14-320[»]
5IMNX-ray2.53A/B/C/D14-320[»]
5IMPX-ray2.04A/B/C/D14-320[»]
5IN8X-ray2.35A/B/C/D14-320[»]
5IVGX-ray1.95A/B/C/D14-320[»]
ProteinModelPortaliQ9UR08.
SMRiQ9UR08.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00177; UER00582.
BRENDAi4.2.3.9. 536.

Miscellaneous databases

EvolutionaryTraceiQ9UR08.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARIS_ASPTE
AccessioniPrimary (citable) accession number: Q9UR08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.