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Protein

Probable proteasome subunit beta type-6

Gene

pam1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of mitotic metaphase/anaphase transition Source: PomBase
  2. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_188566. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_188568. ER-Phagosome pathway.
REACT_188587. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
REACT_215320. Orc1 removal from chromatin.
REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Protein family/group databases

MEROPSiT01.A12.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable proteasome subunit beta type-6 (EC:3.4.25.1)
Gene namesi
Name:pam1
ORF Names:SPAC22F8.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC22F8.06.

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. nucleus Source: PomBase
  3. proteasome core complex, beta-subunit complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Probable proteasome subunit beta type-6PRO_0000148041Add
BLAST

Proteomic databases

MaxQBiQ9UQY2.
PaxDbiQ9UQY2.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).By similarity

Protein-protein interaction databases

BioGridi278540. 2 interactions.
MINTiMINT-4706840.
STRINGi4896.SPAC22F8.06-1.

Structurei

3D structure databases

ProteinModelPortaliQ9UQY2.
SMRiQ9UQY2. Positions 5-225.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091081.
InParanoidiQ9UQY2.
KOiK02732.
OMAiSEYSSEA.
OrthoDBiEOG7VMPGM.
PhylomeDBiQ9UQY2.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UQY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSQFDPYV QNGGTTVAIA GDGFAILAGD TRSVNGYNIN TRFQPRVHEV
60 70 80 90 100
GDDLVIGASG FEADALALVK RIQQRIDLYH DNHERKMSAQ SCACMVRTLL
110 120 130 140 150
YGKRFFPYYV YTTVAGIDKE GKGEIYSFDP VGSYEREWCR AGGSAANFIT
160 170 180 190 200
PFLDNQVNLH NQYVPGSHGK ERKPRRLLKL EEAMKITTDA FTSAGERHIE
210 220
VGDSVLVKII TKEGVETRII PLKKD
Length:225
Mass (Da):25,082
Last modified:May 1, 2000 - v1
Checksum:iBE62FA28F9D7D661
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012135 Genomic DNA. Translation: BAA88692.1.
CU329670 Genomic DNA. Translation: CAB52716.1.
PIRiT38196.
RefSeqiNP_594729.1. NM_001020157.2.

Genome annotation databases

EnsemblFungiiSPAC22F8.06.1; SPAC22F8.06.1:pep; SPAC22F8.06.
GeneIDi2542062.
KEGGispo:SPAC22F8.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012135 Genomic DNA. Translation: BAA88692.1.
CU329670 Genomic DNA. Translation: CAB52716.1.
PIRiT38196.
RefSeqiNP_594729.1. NM_001020157.2.

3D structure databases

ProteinModelPortaliQ9UQY2.
SMRiQ9UQY2. Positions 5-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278540. 2 interactions.
MINTiMINT-4706840.
STRINGi4896.SPAC22F8.06-1.

Protein family/group databases

MEROPSiT01.A12.

Proteomic databases

MaxQBiQ9UQY2.
PaxDbiQ9UQY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC22F8.06.1; SPAC22F8.06.1:pep; SPAC22F8.06.
GeneIDi2542062.
KEGGispo:SPAC22F8.06.

Organism-specific databases

PomBaseiSPAC22F8.06.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091081.
InParanoidiQ9UQY2.
KOiK02732.
OMAiSEYSSEA.
OrthoDBiEOG7VMPGM.
PhylomeDBiQ9UQY2.

Enzyme and pathway databases

ReactomeiREACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_188566. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_188568. ER-Phagosome pathway.
REACT_188587. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
REACT_215320. Orc1 removal from chromatin.
REACT_218991. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_81558. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Miscellaneous databases

NextBioi20803136.
PROiQ9UQY2.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Switch from mitotic to meiotic cell cycle is disturbed by strong activation of Ras-MAP kinase cascade in the fission yeast 26S proteasome-related mutants."
    Kitamura K., Tsujimoto K., Yamashita I., Shimoda C.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 38364 / 968.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSB6_SCHPO
AccessioniPrimary (citable) accession number: Q9UQY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.