Q9UQV4 (LAMP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysosome-associated membrane glycoprotein 3
Short name=LAMP-3
Short name=Lysosomal-associated membrane protein 3

Alternative name(s):
DC-lysosome-associated membrane glycoprotein
Short name=DC LAMP
Protein TSC403
CD_antigen=CD208

Gene names

Name:	LAMP3
Synonyms:	DCLAMP, TSC403

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in dendritic cell function and in adaptive immunity. Ref.2
Subunit structure	Monomer. Ref.6
Subcellular location	Lysosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Note: During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3. Detected on lysosomes in mature dendritic cells. Ref.2 Ref.5
Tissue specificity	Detected in tonsil interdigitating dendritic cells, in spleen, lymph node, Peyer's patches in the small instestine, in thymus medulla and in B cells (at protein level). Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues. Ref.1 Ref.2 Ref.5
Developmental stage	Up-regulated during dendritic cell maturation.
Sequence similarities	Belongs to the LAMP family.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Cytoplasmic vesicle Lysosome Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	alveolar lamellar body membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Potential

Chain

28 – 416

389

Lysosome-associated membrane glycoprotein 3

PRO_0000223695

Regions

Topological domain

28 – 381

354

Lumenal Potential

Transmembrane

382 – 402

21

Helical; Potential

Topological domain

403 – 416

14

Cytoplasmic Potential

Compositional bias

74 – 215

142

Thr-rich

Amino acid modifications

Glycosylation

112

1

N-linked (GlcNAc...) Potential

Glycosylation

158

1

N-linked (GlcNAc...) Potential

Glycosylation

164

1

N-linked (GlcNAc...) Potential

Glycosylation

200

1

N-linked (GlcNAc...) Potential

Glycosylation

232

1

N-linked (GlcNAc...) Potential

Glycosylation

266

1

N-linked (GlcNAc...) Potential

Glycosylation

291

1

N-linked (GlcNAc...) Ref.6

Disulfide bond

237 ↔ 274

Ref.6

Disulfide bond

339 ↔ 376

Ref.6

Natural variations

Natural variant

32

1

E → G. Ref.4
Corresponds to variant rs17853113 [ dbSNP | Ensembl ].

VAR_030827

Natural variant

318

1

I → V. Ref.1 Ref.3
Corresponds to variant rs482912 [ dbSNP | Ensembl ].

VAR_025343

Experimental info

Sequence conflict

145

1

T → A in BAA34533. Ref.1

Sequence conflict

186

1

V → D in BAA34533. Ref.1

Secondary structure

1

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416

Helix Strand Turn

Details...

Beta strand

226 – 232

7

Beta strand

235 – 248

14

Beta strand

251 – 253

3

Beta strand

259 – 262

4

Helix

265 – 267

3

Beta strand

269 – 273

5

Beta strand

276 – 285

10

Beta strand

288 – 296

9

Beta strand

301 – 311

11

Beta strand

313 – 315

3

Beta strand

318 – 323

6

Beta strand

328 – 331

4

Beta strand

337 – 339

3

Beta strand

343 – 346

4

Beta strand

348 – 363

16

Sequences

	Sequence		Length	Mass (Da)
	Q9UQV4 [UniParc]. Last modified February 20, 2007. Version 3. Checksum: D615895D382B8F88	FASTA	416	44,346
	10 20 30 40 50 60 MPRQLSAAAA LFASLAVILH DGSQMRAKAF PETRDYSQPT AAATVQDIKK PVQQPAKQAP 70 80 90 100 110 120 HQTLAARFMD GHITFQTAAT VKIPTTTPAT TKNTATTSPI TYTLVTTQAT PNNSHTAPPV 130 140 150 160 170 180 TEVTVGPSLA PYSLPPTITP PAHTTGTSSS TVSHTTGNTT QPSNQTTLPA TLSIALHKST 190 200 210 220 230 240 TGQKPVQPTH APGTTAAAHN TTRTAAPAST VPGPTLAPQP SSVKTGIYQV LNGSRLCIKA 250 260 270 280 290 300 EMGIQLIVQD KESVFSPRRY FNIDPNATQA SGNCGTRKSN LLLNFQGGFV NLTFTKDEES 310 320 330 340 350 360 YYISEVGAYL TVSDPETIYQ GIKHAVVMFQ TAVGHSFKCV SEQSLQLSAH LQVKTTDVQL 370 380 390 400 410 QAFDFEDDHF GNVDECSSDY TIVLPVIGAI VVGLCLMGMG VYKIRLRCQS SGYQRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a novel human lung-specific gene homologous to lysosomal membrane glycoprotein 1 and 2; significantly increased expression in cancers of various tissues." Ozaki K., Nagata M., Suzuki M., Fujiwara T., Ueda K., Miyoshi Y., Takahashi E., Nakamura Y. Cancer Res. 58:3499-3503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT VAL-318. Tissue: Lung.
[2]	"A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment." de Saint-Vis B.M., Vincent J., Vandenabeele S., Vanbervliet B., Pin J.J., Ait-Yahia S., Patel S., Mattei M.-G., Banchereau J., Zurawski S., Davoust J., Caux C., Lebecque S. Immunity 9:325-336(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-318.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-32. Tissue: Testis.
[5]	"Reciprocal polarization of T and B cells at the immunological synapse." Duchez S., Rodrigues M., Bertrand F., Valitutti S. J. Immunol. 187:4571-4580(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Crystal structure of the conserved domain of the DC lysosomal associated membrane protein: implications for the lysosomal glycocalyx." Wilke S., Krausze J., Bussow K. BMC Biol. 10:62-62(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS) OF 222-381, SUBUNIT, GLYCOSYLATION AT ASN-291, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB013924 mRNA. Translation: BAA34533.1.
AJ005766 mRNA. Translation: CAA06691.1.
CH471052 Genomic DNA. Translation: EAW78337.1.
CH471052 Genomic DNA. Translation: EAW78338.1.
BC032940 mRNA. Translation: AAH32940.1.

IPI

IPI00004307.

RefSeq

NP_055213.2. NM_014398.3.

UniGene

Hs.518448.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AKM	X-ray	2.69	A/B	222-381	[»]

ProteinModelPortal

Q9UQV4.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9UQV4. 1 interaction.

MINT

MINT-7996363.

STRING

9606.ENSP00000265598.

PTM databases

PhosphoSite

Q9UQV4.

Polymorphism databases

DMDM

126302564.

Proteomic databases

PaxDb

Q9UQV4.

PeptideAtlas

Q9UQV4.

PRIDE

Q9UQV4.

Protocols and materials databases

DNASU

27074.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265598; ENSP00000265598; ENSG00000078081.

GeneID

27074.

KEGG

hsa:27074.

UCSC

uc003flh.4. human.

Organism-specific databases

CTD

27074.

GeneCards

GC03M182840.

HGNC

HGNC:14582. LAMP3.

HPA

CAB025133.

MIM

605883. gene.

neXtProt

NX_Q9UQV4.

PharmGKB

PA30286.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG288611.

HOGENOM

HOG000004835.

HOVERGEN

HBG081889.

InParanoid

Q9UQV4.

KO

K06562.

OMA

YYISEVG.

OrthoDB

EOG4NP73T.

PhylomeDB

Q9UQV4.

Gene expression databases

ArrayExpress

Q9UQV4.

Bgee

Q9UQV4.

CleanEx

HS_LAMP3.

Genevestigator

Q9UQV4.

GermOnline

ENSG00000078081. Homo sapiens.

Family and domain databases

InterPro

IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]

PANTHER

PTHR11506. PTHR11506. 1 hit.

Pfam

PF01299. Lamp. 1 hit.
[Graphical view]

PRINTS

PR00336. LYSASSOCTDMP.

PROSITE

PS51407. LAMP_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

27074.

NextBio

49669.

SOURCE

Search...

Entry information

Entry name

LAMP3_HUMAN

Accession

Primary (citable) accession number: Q9UQV4
Secondary accession number(s): D3DNS4, O94781, Q8NEC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	February 20, 2007
Last modified:	May 29, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.