Q9UQR1 (ZN148_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 148

Alternative name(s):
Transcription factor ZBP-89
Zinc finger DNA-binding protein 89

Gene names

Name:	ZNF148
Synonyms:	ZBP89

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Subunit structure	Interacts with HNRPDL. Interacts with the 5FMC complex; the interaction requires association with CHTOP. Ref.6
Subcellular location	Nucleus.
Post-translational modification	Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes By similarity.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular defense response Traceable author statement PubMed 8355710. Source: ProtInc gamete generation Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Traceable author statement PubMed 9685330. Source: ProtInc positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara protein complex assembly Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Traceable author statement PubMed 9685330. Source: ProtInc
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular_function	double-stranded DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from direct assay PubMed 12771217. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara transcription regulatory region sequence-specific DNA binding Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 794	794	Zinc finger protein 148		PRO_0000047427
Regions
	Zinc finger	171 – 193	23	C2H2-type 1
	Zinc finger	199 – 221	23	C2H2-type 2
	Zinc finger	227 – 249	23	C2H2-type 3
	Zinc finger	255 – 278	24	C2H2-type 4
Amino acid modifications
	Modified residue	194	1	Phosphothreonine Ref.8
	Modified residue	250	1	Phosphoserine Ref.8
	Modified residue	305	1	Phosphothreonine By similarity
	Modified residue	306	1	Phosphoserine Ref.7 Ref.8 Ref.9 Ref.11
	Modified residue	412	1	Phosphoserine Ref.8
	Modified residue	607	1	N6-acetyllysine Ref.10
	Modified residue	665	1	Phosphoserine Ref.11
	Modified residue	784	1	Phosphoserine Ref.8 Ref.9 Ref.11
Experimental info
	Sequence conflict	287	1	R → T in CAA15422. Ref.2
	Sequence conflict	348	1	L → V in CAA15422. Ref.2
	Sequence conflict	379	1	E → K in AAB57692. Ref.5
	Sequence conflict	495	1	A → R in CAA15422. Ref.2
	Sequence conflict	616	1	T → N in AAB57692. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9UQR1 [UniParc]. Last modified January 24, 2001. Version 2. Checksum: 50413723459EBD1F	FASTA	794	88,976
	10 20 30 40 50 60 MNIDDKLEGL FLKCGGIDEM QSSRTMVVMG GVSGQSTVSG ELQDSVLQDR SMPHQEILAA 70 80 90 100 110 120 DEVLQESEMR QQDMISHDEL MVHEETVKND EEQMETHERL PQGLQYALNV PISVKQEITF 130 140 150 160 170 180 TDVSEQLMRD KKQIREPVDL QKKKKRKQRS PAKILTINED GSLGLKTPKS HVCEHCNAAF 190 200 210 220 230 240 RTNYHLQRHV FIHTGEKPFQ CSQCDMRFIQ KYLLQRHEKI HTGEKPFRCD ECGMRFIQKY 250 260 270 280 290 300 HMERHKRTHS GEKPYQCEYC LQYFSRTDRV LKHKRMCHEN HDKKLNRCAI KGGLLTSEED 310 320 330 340 350 360 SGFSTSPKDN SLPKKKRQKT EKKSSGMDKE SALDKSDLKK DKNDYLPLYS SSTKVKDEYM 370 380 390 400 410 420 VAEYAVEMPH SSVGGSHLED ASGEIHPPKL VLKKINSKRS LKQPLEQNQT ISPLSTYEES 430 440 450 460 470 480 KVSKYAFELV DKQALLDSEG NADIDQVDNL QEGPSKPVHS STNYDDAMQF LKKKRYLQAA 490 500 510 520 530 540 SNNSREYALN VGTIASQPSV TQAAVASVID ESTTASILES QALNVEIKSN HDKNVIPDEV 550 560 570 580 590 600 LQTLLDHYSH KANGQHEISF SVADTEVTSS ISINSSEVPE VTPSENVGSS SQASSSDKAN 610 620 630 640 650 660 MLQEYSKFLQ QALDRTSQND AYLNSPSLNF VTDNQTLPNQ PAFSSIDKQV YATMPINSFR 670 680 690 700 710 720 SGMNSPLRTT PDKSHFGLIV GDSQHSFPFS GDETNHASAT STQDFLDQVT SQKKAEAQPV 730 740 750 760 770 780 HQAYQMSSFE QPFRAPYHGS RAGIATQFST ANGQVNLRGP GTSAEFSEFP LVNVNDNRAG 790 MTSSPDATTG QTFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human ZBP-89 homolog, located at chromosome 3q21, represses gastrin gene expression." Law D.J., Tarle S.A., Merchant J.L. Mamm. Genome 9:165-167(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human stromelysin gene promoter activity is modulated by transcription factor ZBP-89." Ye S., Whatling C., Watkins H., Henney A. FEBS Lett. 450:268-272(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"The growth-regulating transcription factor ZBP-89 is located at human chromosome 3q21 and mouse chromosome 8B1." Law D.J., Chen X.N., Korenberg J.R., Mortensen E.R., Merchant J.L. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 262-665.
[6]	"Regulation of murine cytochrome c oxidase Vb gene expression during myogenesis: YY-1 and heterogeneous nuclear ribonucleoprotein D-like protein (JKTBP1) reciprocally regulate transcription activity by physical interaction with the BERF-1/ZBP-89 factor." Boopathi E., Lenka N., Prabu S.K., Fang J.-K., Wilkinson F., Atchison M., Giallongo A., Avadhani N.G. J. Biol. Chem. 279:35242-35254(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HNRPDL.
[7]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-194; SER-250; SER-306; SER-412 AND SER-784, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306 AND SER-784, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-607, MASS SPECTROMETRY.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-306; SER-665 AND SER-784, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039019 mRNA. Translation: AAC39926.1. AJ236885 mRNA. Translation: CAA15422.1. CH471052 Genomic DNA. Translation: EAW79394.1. CH471052 Genomic DNA. Translation: EAW79395.1. CH471052 Genomic DNA. Translation: EAW79396.1. CH471052 Genomic DNA. Translation: EAW79398.1. BC050260 mRNA. Translation: AAH50260.1. U96633 Genomic DNA. Translation: AAB57692.1.
IPI	IPI00010833.
RefSeq	NP_068799.2. NM_021964.2.
UniGene	Hs.592591.
3D structure databases
ProteinModelPortal	Q9UQR1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UQR1. 1 interaction.
STRING	9606.ENSP00000353863.
PTM databases
PhosphoSite	Q9UQR1.
Polymorphism databases
DMDM	12643385.
Proteomic databases
PaxDb	Q9UQR1.
PRIDE	Q9UQR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360647; ENSP00000353863; ENSG00000163848. ENST00000484491; ENSP00000420335; ENSG00000163848. ENST00000485866; ENSP00000420448; ENSG00000163848. ENST00000492394; ENSP00000419322; ENSG00000163848.
GeneID	7707.
KEGG	hsa:7707.
UCSC	uc003ehx.4. human.
Organism-specific databases
CTD	7707.
GeneCards	GC03M124944.
HGNC	HGNC:12933. ZNF148.
HPA	CAB017486. HPA001656.
MIM	601897. gene.
neXtProt	NX_Q9UQR1.
PharmGKB	PA37520.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000293204.
HOVERGEN	HBG054247.
InParanoid	Q9UQR1.
OMA	NDAYLNN.
OrthoDB	EOG43N7C2.
PhylomeDB	Q9UQR1.
Enzyme and pathway databases
SignaLink	Q9UQR1.
Gene expression databases
ArrayExpress	Q9UQR1.
Bgee	Q9UQR1.
CleanEx	HS_ZNF148.
Genevestigator	Q9UQR1.
GermOnline	ENSG00000163848. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 4 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 4 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7707.
NextBio	29880.
SOURCE	Search...

Entry information

Entry name

ZN148_HUMAN

Accession

Primary (citable) accession number: Q9UQR1
Secondary accession number(s): D3DN27 , O00389, O43591, Q58EY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 24, 2001
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.