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Q9UQQ1 (NALDL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylated-alpha-linked acidic dipeptidase-like protein

Short name=NAALADase L
EC=3.4.17.21
Alternative name(s):
100 kDa ileum brush border membrane protein
Short name=I100
Ileal dipeptidylpeptidase
Gene names
Name:NAALADL1
Synonyms:NAALADASEL, NAALADL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length740 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

NAALADase-like activity unknown. Has no NAAG hydrolyzing activity. Exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC By similarity.

Catalytic activity

Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subcellular location

Apical cell membrane; Single-pass type II membrane protein. Note: Ileal brush border membrane.

Tissue specificity

Mainly expressed in the distal small intestine. Also expressed in the spleen and testis. Weak expression in the brain, locating mainly to the brain stem, amygdala, thalamus and ventral striatum. Isoform 2 and 3 isoform 3 are found in the small intestine and colon.

Sequence similarities

Belongs to the peptidase M28 family. M28B subfamily.

Sequence caution

The sequence AAB87645.1 differs from that shown. Reason: Frameshift at positions 686 and 693.

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9UQQ1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9UQQ1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     161-201: Missing.
Isoform 3 (identifier: Q9UQQ1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     296-330: Missing.
Isoform 4 (identifier: Q9UQQ1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     359-359: P → PGEPSSCCLHPRPLLCSGCRCPHPALPLPPPSPAPPAHLSLSSGSLPLFLWP
Isoform 5 (identifier: Q9UQQ1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     502-522: PSLGSLGAGSDYAPFVHFLGI → PRLQQPSGCGPDSGECDSPAQ
     523-740: Missing.
Isoform 6 (identifier: Q9UQQ1-6)

The sequence of this isoform differs from the canonical sequence as follows:
     502-543: PSLGSLGAGS...SKTSARIYPT → PRARLQPGSP...IRLWPGQRGV
     544-740: Missing.
Isoform 7 (identifier: Q9UQQ1-7)

The sequence of this isoform differs from the canonical sequence as follows:
     562-579: FSSHQAVARTAGSVILRL → FEEGDKGHPETRTGEAED
     580-740: Missing.
Isoform 8 (identifier: Q9UQQ1-8)

The sequence of this isoform differs from the canonical sequence as follows:
     619-635: GPLVTAVEKFEAEAAAL → GMHSPDPEVWGALHPHD
     636-740: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 740740N-acetylated-alpha-linked acidic dipeptidase-like protein
PRO_0000174123

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2822Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 740712Extracellular Potential
Region263 – 579317NAALADase

Sites

Active site4161Nucleophile; for NAALADase activity By similarity
Active site6171Charge relay system Potential
Active site6571Charge relay system Potential
Active site6801Charge relay system Potential
Metal binding3681Zinc 2 By similarity
Metal binding3781Zinc 1 By similarity
Metal binding3781Zinc 2 By similarity
Metal binding4171Zinc 1 By similarity
Metal binding4451Zinc 2 By similarity
Metal binding5451Zinc 1 By similarity

Amino acid modifications

Glycosylation1361N-linked (GlcNAc...) Potential
Glycosylation2741N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation4511N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence161 – 20141Missing in isoform 2.
VSP_005343
Alternative sequence296 – 33035Missing in isoform 3.
VSP_005344
Alternative sequence3591P → PGEPSSCCLHPRPLLCSGCR CPHPALPLPPPSPAPPAHLS LSSGSLPLFLWP in isoform 4.
VSP_005345
Alternative sequence502 – 54342PSLGS…RIYPT → PRARLQPGSPPTTQPLTPLT MWTSFWTRASAAIRLWPGQR GV in isoform 6.
VSP_005348
Alternative sequence502 – 52221PSLGS…HFLGI → PRLQQPSGCGPDSGECDSPA Q in isoform 5.
VSP_005346
Alternative sequence523 – 740218Missing in isoform 5.
VSP_005347
Alternative sequence544 – 740197Missing in isoform 6.
VSP_005349
Alternative sequence562 – 57918FSSHQ…VILRL → FEEGDKGHPETRTGEAED in isoform 7.
VSP_005350
Alternative sequence580 – 740161Missing in isoform 7.
VSP_005351
Alternative sequence619 – 63517GPLVT…EAAAL → GMHSPDPEVWGALHPHD in isoform 8.
VSP_005352
Alternative sequence636 – 740105Missing in isoform 8.
VSP_005353
Natural variant21Q → H.
Corresponds to variant rs35422506 [ dbSNP | Ensembl ].
VAR_057155
Natural variant3981K → T.
Corresponds to variant rs12223986 [ dbSNP | Ensembl ].
VAR_057156
Natural variant6111L → V.
Corresponds to variant rs36053340 [ dbSNP | Ensembl ].
VAR_057157

Experimental info

Sequence conflict420 – 4245LIGST → SPGLQ in AAB87645. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 018D59835F2C48AC

FASTA74080,558
        10         20         30         40         50         60 
MQWTKVLGLG LGAAALLGLG IILGHFAIPK KANSLAPQDL DLEILETVMG QLDAHRIREN 

        70         80         90        100        110        120 
LRELSREPHL ASSPRDEDLV QLLLQRWKDP ESGLDSAEAS TYEVLLSFPS QEQPNVVDIV 

       130        140        150        160        170        180 
GPTGGIIHSC HRTEENVTGE QGGPDVVQPY AAYAPSGTPQ GLLVYANRGA EEDFKELQTQ 

       190        200        210        220        230        240 
GIKLEGTIAL TRYGGVGRGA KAVNAAKHGV AGVLVYTDPA DINDGLSSPD ETFPNSWYLP 

       250        260        270        280        290        300 
PSGVERGSYY EYFGDPLTPY LPAVPSSFRV DLANVSGFPP IPTQPIGFQD ARDLLCNLNG 

       310        320        330        340        350        360 
TLAPATWQGA LGCHYRLGPG FRPDGDFPAD SQVNVSVYNR LELRNSSNVL GIIRGAVEPD 

       370        380        390        400        410        420 
RYVLYGNHRD SWVHGAVDPS SGTAVLLELS RVLGTLLKKG TWRPRRSIVF ASWGAEEFGL 

       430        440        450        460        470        480 
IGSTEFTEEF FNKLQERTVA YINVDISVFA NATLRVQGTP PVQSVVFSAT KEIRSPGPGD 

       490        500        510        520        530        540 
LSIYDNWIRY FNRSSPVYGL VPSLGSLGAG SDYAPFVHFL GISSMDIAYT YDRSKTSARI 

       550        560        570        580        590        600 
YPTYHTAFDT FDYVDKFLDP GFSSHQAVAR TAGSVILRLS DSFFLPLKVS DYSETLRSFL 

       610        620        630        640        650        660 
QAAQQDLGAL LEQHSISLGP LVTAVEKFEA EAAALGQRIS TLQKGSPDPL QVRMLNDQLM 

       670        680        690        700        710        720 
LLERTFLNPR AFPEERYYSH VLWAPRTGSV VTFPGLSNAC SRARDTASGS EAWAEVQRQL 

       730        740 
SIVVTALEGA AATLRPVADL 

« Hide

Isoform 2 [UniParc].

Checksum: BDF2C67F921ED4CA
Show »

FASTA69976,195
Isoform 3 [UniParc].

Checksum: B043A0DBBDEBFB8D
Show »

FASTA70576,887
Isoform 4 [UniParc].

Checksum: 8872DB91F3669FD7
Show »

FASTA79185,832
Isoform 5 [UniParc].

Checksum: D9C30160F01AC93B
Show »

FASTA52256,573
Isoform 6 [UniParc].

Checksum: 3DEF010A089357C9
Show »

FASTA54359,132
Isoform 7 [UniParc].

Checksum: 62FAEF2C2C593E0F
Show »

FASTA57963,077
Isoform 8 [UniParc].

Checksum: 1388AE658E962A2C
Show »

FASTA63569,188

References

« Hide 'large scale' references
[1]"Isolation and expression of novel human glutamate carboxypeptidases with N-acetylated alpha-linked acidic dipeptidase and dipeptidyl peptidase IV activity."
Pangalos M.N., Neefs J.-M., Somers M., Verhasselt P., Bekkers M., van der Helm L., Fraiponts E., Ashton D., Gordon R.D.
J. Biol. Chem. 274:8470-8483(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5; 6; 7 AND 8).
Tissue: Small intestine.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Cloning and characterization of a novel peptidase from rat and human ileum."
Shneider B.L., Thevananther S., Moyer M.S., Walters H.C., Rinaldo P., Devarajan P., Sun A.Q., Dawson P.A., Ananthanarayanan M.
J. Biol. Chem. 272:31006-31015(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 419-740.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ012371 mRNA. Translation: CAB39968.1.
AP003068 Genomic DNA. No translation available.
AF010141 mRNA. Translation: AAB87645.1. Frameshift.
RefSeqNP_005459.2. NM_005468.2.
UniGeneHs.13967.

3D structure databases

ProteinModelPortalQ9UQQ1.
SMRQ9UQQ1. Positions 48-737.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000351484.

Protein family/group databases

MEROPSM28.011.

PTM databases

PhosphoSiteQ9UQQ1.

Polymorphism databases

DMDM313104139.

Proteomic databases

PaxDbQ9UQQ1.
PRIDEQ9UQQ1.

Protocols and materials databases

DNASU10004.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000340252; ENSP00000344244; ENSG00000168060. [Q9UQQ1-4]
ENST00000355369; ENSP00000347530; ENSG00000168060. [Q9UQQ1-5]
ENST00000355721; ENSP00000347955; ENSG00000168060. [Q9UQQ1-2]
ENST00000358658; ENSP00000351484; ENSG00000168060. [Q9UQQ1-1]
GeneID10004.
KEGGhsa:10004.
UCSCuc001ocn.3. human. [Q9UQQ1-1]

Organism-specific databases

CTD10004.
GeneCardsGC11M064812.
H-InvDBHIX0035851.
HGNCHGNC:23536. NAALADL1.
HPAHPA047123.
MIM602640. gene.
neXtProtNX_Q9UQQ1.
PharmGKBPA134867982.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2234.
HOGENOMHOG000211921.
HOVERGENHBG051639.
KOK01301.
OMARGAEEDF.
OrthoDBEOG7QK0BC.
PhylomeDBQ9UQQ1.
TreeFamTF312981.

Gene expression databases

ArrayExpressQ9UQQ1.
BgeeQ9UQQ1.
CleanExHS_NAALADL1.
GenevestigatorQ9UQQ1.

Family and domain databases

Gene3D1.20.930.40. 1 hit.
InterProIPR007484. Peptidase_M28.
IPR003137. Protease-assoc_domain.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMSSF47672. SSF47672. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi10004.
NextBio37785.
PROQ9UQQ1.
SOURCESearch...

Entry information

Entry nameNALDL_HUMAN
AccessionPrimary (citable) accession number: Q9UQQ1
Secondary accession number(s): C9J8A1 expand/collapse secondary AC list , C9J964, C9JL35, C9JSN0, O43176
Entry history
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: November 30, 2010
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM