Calcium/calmodulin-dependent protein kinase type II subunit alpha

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

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Molecule processing

Regions

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Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

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Q9UQM7 (KCC2A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Calcium/calmodulin-dependent protein kinase type II subunit alpha
Short name=CaM kinase II subunit alpha
Short name=CaMK-II subunit alpha
EC=2.7.11.17

Gene names

Name:	CAMK2A
Synonyms:	CAMKA, KIAA0968

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state By similarity.
Subunit structure	CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1 By similarity. Ref.4
Subcellular location	Cell junction › synapse › presynaptic cell membrane By similarity. Cell junction › synapse By similarity. Note: Postsynaptic lipid rafts By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence BAA76812.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Keywords
Cellular component	Cell junction Cell membrane Membrane Synapse
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome positive regulation of NF-kappaB transcription factor activity Inferred from mutant phenotype. Source: UniProtKB synaptic transmission Traceable author statement. Source: Reactome
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytosol Traceable author statement. Source: Reactome endocytic vesicle membrane Traceable author statement. Source: Reactome nucleoplasm Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 478

478

Calcium/calmodulin-dependent protein kinase type II subunit alpha

PRO_0000086091

Domain

13 – 271

259

Protein kinase

Nucleotide binding

19 – 27

ATP By similarity

Region

290 – 300

Calmodulin-binding

Region

310 – 320

Interaction with BAALC By similarity

Active site

135

Proton acceptor By similarity

Binding site

ATP By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

275

Phosphoserine By similarity

Modified residue

286

Phosphothreonine; by autocatalysis By similarity

Modified residue

333

Phosphoserine By similarity

Modified residue

334

Phosphothreonine By similarity

Modified residue

337

Phosphothreonine By similarity

Alternative sequence

328

K → KKRKSSSSVQLM in isoform B.

VSP_004766

Sequence conflict

365

D → G in AAD30558. Ref.1

Sequence conflict

365

D → G in AAD30559. Ref.1

478

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform A [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 208143A311BA9262 Show »	FASTA	478	54,088
10 20 30 40 50 60 MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE 70 80 90 100 110 120 ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL 130 140 150 160 170 180 EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL 190 200 210 220 230 240 SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV 250 260 270 280 290 300 TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK 310 320 330 340 350 360 GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA 370 380 390 400 410 420 ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH 430 440 450 460 470 LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH « Hide
Isoform B [UniParc]. Checksum: 41E67A1E15EB142B Show »	FASTA	489	55,320
10 20 30 40 50 60 MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE 70 80 90 100 110 120 ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL 130 140 150 160 170 180 EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL 190 200 210 220 230 240 SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV 250 260 270 280 290 300 TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK 310 320 330 340 350 360 GAILTTMLAT RNFSGGKSGG NKKSDGVKKR KSSSSVQLME SSESTNTTIE DEDTKVRKQE 370 380 390 400 410 420 IIKVTEQLIE AISNGDFESY TKMCDPGMTA FEPEALGNLV EGLDFHRFYF ENLWSRNSKP 430 440 450 460 470 480 VHTTILNPHI HLMGDESACI AYIRITQYLD AGGIPRTAQS EETRVWHRRD GKWQIVHFHR SGAPSVLPH « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human calcium/calmodulin-dependent protein kinase II: cDNA cloning and gene analysis." Li G.Y., Cooper N.G.F. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B). Tissue: Brain.
[2]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed: 10231032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation." Krapivinsky G., Medina I., Krapivinsky L., Gapon S., Clapham D.E. Neuron 43:563-574(2004) [PubMed: 15312654] [Abstract] Cited for: INTERACTION WITH MPDZ.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

AF145710 mRNA. Translation: AAD30558.1.
AF145711 mRNA. Translation: AAD30559.1.
AB023185 mRNA. Translation: BAA76812.1. Different initiation.
AC011372 Genomic DNA. No translation available.

IPI

IPI00215715.
IPI00550056.

RefSeq

NP_741960.1. NM_171825.2.

UniGene

Hs.725992.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VZ6	X-ray	2.30	A/B	13-302	[»]
3SOA	X-ray	3.55	A	1-474	[»]

ProteinModelPortal

Q9UQM7.

SMR

Q9UQM7. Positions 9-300, 336-474.

ModBase

Search...

DIP

DIP-39705N.

IntAct

Q9UQM7. 1 interaction.

MINT

MINT-95446.

STRING

Q9UQM7.

PhosphoSite

Q9UQM7.

DMDM

296434552.

PRIDE

Q9UQM7.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENST00000348628; ENSP00000261793; ENSG00000070808.

GeneID

815.

KEGG

hsa:815.

CTD

815.

GeneCards

GC05M149579.

HGNC

HGNC:1460. CAMK2A.

HPA

CAB004330.

MIM

114078. gene.

neXtProt

NX_Q9UQM7.

HUGE

Search...

GenAtlas

Search...

GeneTree

ENSGT00550000074354.

HOVERGEN

HBG108055.

OMA

ICTRFTE.

OrthoDB

EOG42JNR7.

PhylomeDB

Q9UQM7.

BRENDA

2.7.11.17. 2681.

Pathway_Interaction_DB

fgf_pathway. FGF signaling pathway.
ifngpathway. IFN-gamma pathway.
wnt_calcium_pathway. Noncanonical Wnt signaling pathway.
tgfbrpathway. TGF-beta receptor signaling.
pi3kplctrkpathway. Trk receptor signaling mediated by PI3K and PLC-gamma.

Reactome

REACT_13685. Neuronal System.
REACT_6900. Immune System.

ArrayExpress

Q9UQM7.

Bgee

Q9UQM7.

CleanEx

HS_CAMK2A.

Genevestigator

Q9UQM7.

InterPro

IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR013543. Ca/CaM-dep_prot_kinase-assoc.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR002290. Ser/Thr_kinase_dom.
[Graphical view]

K04515.

PANTHER

PTHR24347. PTHR24347. 1 hit.

Pfam

PF08332. CaMKII_AD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

NextBio

3304.

SOURCE

Search...

Entry name

KCC2A_HUMAN

Accession

Primary (citable) accession number: Q9UQM7
Secondary accession number(s): Q9UL21, Q9Y2H4, Q9Y352

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9UQM7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9UQM7-2)

The sequence of this isoform differs from the canonical sequence as follows:
328-328: K → KKRKSSSSVQLM