Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9UQM7

- KCC2A_HUMAN

UniProt

Q9UQM7 - KCC2A_HUMAN

Protein

Calcium/calmodulin-dependent protein kinase type II subunit alpha

Gene

CAMK2A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei42 – 421ATPPROSITE-ProRule annotation
    Active sitei135 – 1351Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: UniProtKB-EC
    3. kinase activity Source: UniProtKB
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. calcium ion transport Source: Ensembl
    2. cytokine-mediated signaling pathway Source: Reactome
    3. G1/S transition of mitotic cell cycle Source: Ensembl
    4. interferon-gamma-mediated signaling pathway Source: Reactome
    5. positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
    6. protein autophosphorylation Source: Ensembl
    7. protein phosphorylation Source: UniProtKB
    8. regulation of neuronal synaptic plasticity Source: Ensembl
    9. regulation of neurotransmitter secretion Source: Ensembl
    10. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.17. 2681.
    ReactomeiREACT_172761. Ca2+ pathway.
    REACT_18307. Trafficking of AMPA receptors.
    REACT_200775. HSF1-dependent transactivation.
    REACT_20546. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_20568. CREB phosphorylation through the activation of Ras.
    REACT_20594. Unblocking of NMDA receptor, glutamate binding and activation.
    REACT_20642. CREB phosphorylation through the activation of CaMKII.
    REACT_25078. Interferon gamma signaling.
    SignaLinkiQ9UQM7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent protein kinase type II subunit alpha (EC:2.7.11.17)
    Short name:
    CaM kinase II subunit alpha
    Short name:
    CaMK-II subunit alpha
    Gene namesi
    Name:CAMK2A
    Synonyms:CAMKA, KIAA0968
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:1460. CAMK2A.

    Subcellular locationi

    Cell junctionsynapsepresynaptic cell membrane By similarity. Cell junctionsynapse By similarity
    Note: Postsynaptic lipid rafts.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytosol Source: Reactome
    3. endocytic vesicle membrane Source: Reactome
    4. nucleoplasm Source: Reactome
    5. nucleus Source: UniProt
    6. plasma membrane Source: Reactome
    7. presynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Synapse

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA90.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Calcium/calmodulin-dependent protein kinase type II subunit alphaPRO_0000086091Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131PhosphotyrosineBy similarity
    Modified residuei286 – 2861Phosphothreonine; by autocatalysisBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9UQM7.
    PaxDbiQ9UQM7.
    PRIDEiQ9UQM7.

    PTM databases

    PhosphoSiteiQ9UQM7.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9UQM7.
    BgeeiQ9UQM7.
    CleanExiHS_CAMK2A.
    GenevestigatoriQ9UQM7.

    Organism-specific databases

    HPAiCAB004330.

    Interactioni

    Subunit structurei

    CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GRIN2BQ132243EBI-1383687,EBI-2256942

    Protein-protein interaction databases

    BioGridi107265. 36 interactions.
    DIPiDIP-39705N.
    IntActiQ9UQM7. 9 interactions.
    MINTiMINT-95446.
    STRINGi9606.ENSP00000381412.

    Structurei

    Secondary structure

    1
    478
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 208
    Beta strandi26 – 327
    Helixi33 – 353
    Beta strandi37 – 459
    Helixi46 – 483
    Helixi51 – 6616
    Beta strandi75 – 806
    Beta strandi82 – 909
    Helixi97 – 1026
    Helixi109 – 12820
    Helixi138 – 1403
    Beta strandi141 – 1444
    Beta strandi152 – 1543
    Helixi177 – 1793
    Helixi182 – 1854
    Helixi193 – 20816
    Helixi218 – 22710
    Helixi236 – 2394
    Helixi242 – 25110
    Turni256 – 2583
    Helixi262 – 2654
    Helixi269 – 2724
    Helixi274 – 2774
    Helixi284 – 29815

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VZ6X-ray2.30A/B13-302[»]
    3SOAX-ray3.55A1-474[»]
    ProteinModelPortaliQ9UQM7.
    SMRiQ9UQM7. Positions 7-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UQM7.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 271259Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni290 – 30011Calmodulin-bindingAdd
    BLAST
    Regioni310 – 32011Interaction with BAALCBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233016.
    HOVERGENiHBG108055.
    KOiK04515.
    OMAiITCTRFS.
    OrthoDBiEOG7ZD1VM.
    PhylomeDBiQ9UQM7.
    TreeFamiTF315229.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR013543. Ca/CaM-dep_prot_kinase-assoc.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF08332. CaMKII_AD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q9UQM7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS    50
    ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED 100
    IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA 150
    AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG 200
    VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK 250
    MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK 300
    GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI 350
    IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE 400
    NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE 450
    ETRVWHRRDG KWQIVHFHRS GAPSVLPH 478
    Length:478
    Mass (Da):54,088
    Last modified:May 18, 2010 - v2
    Checksum:i208143A311BA9262
    GO
    Isoform B (identifier: Q9UQM7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         328-328: K → KKRKSSSSVQLM

    Show »
    Length:489
    Mass (Da):55,320
    Checksum:i41E67A1E15EB142B
    GO

    Sequence cautioni

    The sequence BAA76812.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti365 – 3651D → G in AAD30558. 1 PublicationCurated
    Sequence conflicti365 – 3651D → G in AAD30559. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei328 – 3281K → KKRKSSSSVQLM in isoform B. 1 PublicationVSP_004766

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF145710 mRNA. Translation: AAD30558.1.
    AF145711 mRNA. Translation: AAD30559.1.
    AB023185 mRNA. Translation: BAA76812.1. Different initiation.
    AC011372 Genomic DNA. No translation available.
    CCDSiCCDS43386.1. [Q9UQM7-1]
    CCDS43387.1. [Q9UQM7-2]
    RefSeqiNP_741960.1. NM_171825.2. [Q9UQM7-1]
    UniGeneiHs.743976.

    Genome annotation databases

    EnsembliENST00000348628; ENSP00000261793; ENSG00000070808. [Q9UQM7-1]
    ENST00000398376; ENSP00000381412; ENSG00000070808. [Q9UQM7-2]
    GeneIDi815.
    KEGGihsa:815.
    UCSCiuc003lru.2. human. [Q9UQM7-1]

    Polymorphism databases

    DMDMi296434552.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF145710 mRNA. Translation: AAD30558.1 .
    AF145711 mRNA. Translation: AAD30559.1 .
    AB023185 mRNA. Translation: BAA76812.1 . Different initiation.
    AC011372 Genomic DNA. No translation available.
    CCDSi CCDS43386.1. [Q9UQM7-1 ]
    CCDS43387.1. [Q9UQM7-2 ]
    RefSeqi NP_741960.1. NM_171825.2. [Q9UQM7-1 ]
    UniGenei Hs.743976.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VZ6 X-ray 2.30 A/B 13-302 [» ]
    3SOA X-ray 3.55 A 1-474 [» ]
    ProteinModelPortali Q9UQM7.
    SMRi Q9UQM7. Positions 7-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107265. 36 interactions.
    DIPi DIP-39705N.
    IntActi Q9UQM7. 9 interactions.
    MINTi MINT-95446.
    STRINGi 9606.ENSP00000381412.

    Chemistry

    BindingDBi Q9UQM7.
    ChEMBLi CHEMBL2097164.
    GuidetoPHARMACOLOGYi 1555.

    PTM databases

    PhosphoSitei Q9UQM7.

    Polymorphism databases

    DMDMi 296434552.

    Proteomic databases

    MaxQBi Q9UQM7.
    PaxDbi Q9UQM7.
    PRIDEi Q9UQM7.

    Protocols and materials databases

    DNASUi 815.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000348628 ; ENSP00000261793 ; ENSG00000070808 . [Q9UQM7-1 ]
    ENST00000398376 ; ENSP00000381412 ; ENSG00000070808 . [Q9UQM7-2 ]
    GeneIDi 815.
    KEGGi hsa:815.
    UCSCi uc003lru.2. human. [Q9UQM7-1 ]

    Organism-specific databases

    CTDi 815.
    GeneCardsi GC05M149579.
    HGNCi HGNC:1460. CAMK2A.
    HPAi CAB004330.
    MIMi 114078. gene.
    neXtProti NX_Q9UQM7.
    PharmGKBi PA90.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233016.
    HOVERGENi HBG108055.
    KOi K04515.
    OMAi ITCTRFS.
    OrthoDBi EOG7ZD1VM.
    PhylomeDBi Q9UQM7.
    TreeFami TF315229.

    Enzyme and pathway databases

    BRENDAi 2.7.11.17. 2681.
    Reactomei REACT_172761. Ca2+ pathway.
    REACT_18307. Trafficking of AMPA receptors.
    REACT_200775. HSF1-dependent transactivation.
    REACT_20546. Ras activation uopn Ca2+ infux through NMDA receptor.
    REACT_20568. CREB phosphorylation through the activation of Ras.
    REACT_20594. Unblocking of NMDA receptor, glutamate binding and activation.
    REACT_20642. CREB phosphorylation through the activation of CaMKII.
    REACT_25078. Interferon gamma signaling.
    SignaLinki Q9UQM7.

    Miscellaneous databases

    ChiTaRSi CAMK2A. human.
    EvolutionaryTracei Q9UQM7.
    GeneWikii CAMK2A.
    GenomeRNAii 815.
    NextBioi 3304.
    PROi Q9UQM7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UQM7.
    Bgeei Q9UQM7.
    CleanExi HS_CAMK2A.
    Genevestigatori Q9UQM7.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR013543. Ca/CaM-dep_prot_kinase-assoc.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF08332. CaMKII_AD. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human calcium/calmodulin-dependent protein kinase II: cDNA cloning and gene analysis."
      Li G.Y., Cooper N.G.F.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
      Tissue: Brain.
    2. "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation."
      Krapivinsky G., Medina I., Krapivinsky L., Gapon S., Clapham D.E.
      Neuron 43:563-574(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MPDZ.

    Entry informationi

    Entry nameiKCC2A_HUMAN
    AccessioniPrimary (citable) accession number: Q9UQM7
    Secondary accession number(s): Q9UL21, Q9Y2H4, Q9Y352
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3