Q9UQ07 (MOK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAPK/MAK/MRK overlapping kinase
EC=2.7.11.22

Alternative name(s):
MOK protein kinase
Renal tumor antigen 1
Short name=RAGE-1

Gene names

Name:	MOK
Synonyms:	RAGE, RAGE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Able to phosphorylate several exogenous substrates and to undergo autophosphorylation By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation appears to increase the enzymatic activity By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAB38087.1 differs from that shown. Reason: Frameshift at position 299.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Traceable author statement Ref.1. Source: ProtInc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UQ07-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UQ07-2)

The sequence of this isoform differs from the canonical sequence as follows:
     232-419: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9UQ07-3)

Also known as: RAGE-1 ORF5; RAGE-2 ORF5; RAGE-3 ORF5;

The sequence of this isoform differs from the canonical sequence as follows:
     1-346: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9UQ07-4)

Also known as: RAGE-4 ORF3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     290-329: KTEKRALGSH...ISKEGRKQKQ → TQNGSEDEAS...LPGRSVTLLV
     330-419: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	MAPK/MAK/MRK overlapping kinase		PRO_0000086341
Regions
	Domain	4 – 285	282	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 346	346	Missing in isoform 3.		VSP_009143
	Alternative sequence	1 – 234	234	Missing in isoform 4.		VSP_009142
	Alternative sequence	232 – 419	188	Missing in isoform 2.		VSP_009144
	Alternative sequence	290 – 329	40	KTEKR…RKQKQ → TQNGSEDEASAVLLPIQTRS SLNPLLSTCMLPGRSVTLLV in isoform 4.		VSP_009145
	Alternative sequence	330 – 419	90	Missing in isoform 4.		VSP_009146
	Natural variant	38	1	R → H. Ref.4 Corresponds to variant rs34114580 [ dbSNP | Ensembl ].		VAR_042011
	Natural variant	86	1	D → N. Ref.4 Corresponds to variant rs34084056 [ dbSNP | Ensembl ].		VAR_042012
	Natural variant	230	1	K → R. Ref.4 Corresponds to variant rs34965156 [ dbSNP | Ensembl ].		VAR_042013
	Natural variant	248	1	P → S. Ref.4 Corresponds to variant rs34299975 [ dbSNP | Ensembl ].		VAR_042014
	Natural variant	272	1	E → D in a breast pleomorphic lobular carcinoma sample; somatic mutation. Ref.4		VAR_042015
	Natural variant	398	1	Q → R. Ref.4 Corresponds to variant rs2236493 [ dbSNP | Ensembl ].		VAR_024576

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CDA7D9C1E80AAE8F	FASTA	419	48,014
	10 20 30 40 50 60 MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQRFE SIEQVNNLRE IQALRRLNPH 70 80 90 100 110 120 PNILMLHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRYPL SEKKIMHYMY QLCKSLDHIH 130 140 150 160 170 180 RNGIFHRDVK PENILIKQDV LKLGDFGSCR SVYSKQPYTE YISTRWYRAP ECLLTDGFYT 190 200 210 220 230 240 YKMDLWSAGC VFYEIASLQP LFPGVNELDQ ISKIHDVIGT PAQKILTKFK QSRAMNFDFP 250 260 270 280 290 300 FKKGSGIPLL TTNLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQEQRK TEKRALGSHR 310 320 330 340 350 360 KAGFPEHPVA PEPLSNSCQI SKEGRKQKQS LKQEEDRPKR RGPAYVMELP KLKLSGVVRL 370 380 390 400 410 SSYSSPTLQS VLGSGTNGRV PVLRPLKCIP ASKKTDPQKD LKPAPQQCRL PTIVRKGGR
	Isoform 2 [UniParc]. Checksum: D0665FAE077D3B76	FASTA	231	27,120
	10 20 30 40 50 60 MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQRFE SIEQVNNLRE IQALRRLNPH 70 80 90 100 110 120 PNILMLHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRYPL SEKKIMHYMY QLCKSLDHIH 130 140 150 160 170 180 RNGIFHRDVK PENILIKQDV LKLGDFGSCR SVYSKQPYTE YISTRWYRAP ECLLTDGFYT 190 200 210 220 230 YKMDLWSAGC VFYEIASLQP LFPGVNELDQ ISKIHDVIGT PAQKILTKFK Q
	Isoform 3 (RAGE-1 ORF5) (RAGE-2 ORF5) (RAGE-3 ORF5) [UniParc]. Checksum: 38B1376ECFEDF351	FASTA	73	7,879
	10 20 30 40 50 60 MELPKLKLSG VVRLSSYSSP TLQSVLGSGT NGRVPVLRPL KCIPASKKTD PQKDLKPAPQ 70 QCRLPTIVRK GGR
	Isoform 4 (RAGE-4 ORF3) [UniParc]. Checksum: 8B155074ED025D9A	FASTA	95	10,482
	10 20 30 40 50 60 MNFDFPFKKG SGIPLLTTNL SPQCLSLLHA MVAYDPDERI AAHQALQHPY FQEQRTQNGS 70 80 90 EDEASAVLLP IQTRSSLNPL LSTCMLPGRS VTLLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel member of the MAP kinase superfamily." Miyata Y., Akashi M., Nishida E. Genes Cells 4:299-309(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma." Gaugler B., Brouwenstijn N., Vantomme V., Szikora J.-P., Van der Spek C.W., Patard J.-J., Boon T., Schrier P., Van den Eynde B.J. Immunogenetics 44:323-330(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4). Tissue: Renal cell carcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain, Lung and Testis.
[4]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-38; ASN-86; ARG-230; SER-248; ASP-272 AND ARG-398.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022694 mRNA. Translation: BAA81688.1. U46191 mRNA. Translation: AAB38079.1. U46192 mRNA. Translation: AAB38082.1. U46193 mRNA. Translation: AAB38085.1. U46194 mRNA. Translation: AAB38087.1. Frameshift. BC053536 mRNA. Translation: AAH53536.1.
IPI	IPI00398648. IPI00398649. IPI00413657. IPI00472352.
RefSeq	NP_001258940.1. NM_001272011.1. NP_055041.1. NM_014226.2.
UniGene	Hs.104119.
3D structure databases
ProteinModelPortal	Q9UQ07.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UQ07. 1 interaction.
MINT	MINT-4777240.
STRING	9606.ENSP00000355304.
PTM databases
PhosphoSite	Q9UQ07.
Polymorphism databases
DMDM	41017258.
Proteomic databases
PaxDb	Q9UQ07.
PRIDE	Q9UQ07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000193029; ENSP00000193029; ENSG00000080823. ENST00000361847; ENSP00000355304; ENSG00000080823.
GeneID	5891.
KEGG	hsa:5891.
UCSC	uc001ylm.3. human.
Organism-specific databases
CTD	5891.
GeneCards	GC14M102690.
H-InvDB	HIX0011985.
HGNC	HGNC:9833. MOK.
HPA	HPA027282. HPA027292.
MIM	605762. gene.
neXtProt	NX_Q9UQ07.
PharmGKB	PA34187.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
HOVERGEN	HBG106271.
KO	K08830.
OMA	ILMLHEV.
PhylomeDB	Q9UQ07.
Enzyme and pathway databases
SignaLink	Q9UQ07.
Gene expression databases
ArrayExpress	Q9UQ07.
Bgee	Q9UQ07.
CleanEx	HS_RAGE.
Genevestigator	Q9UQ07.
GermOnline	ENSG00000080823. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MOK. human.
GenomeRNAi	5891.
NextBio	22916.
SOURCE	Search...

Entry information

Entry name

MOK_HUMAN

Accession

Primary (citable) accession number: Q9UQ07
Secondary accession number(s): Q92790, Q93067

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.