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Protein

Microtubule-associated protein RP/EB family member 3

Gene

MAPRE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). May play a role in cell migration (By similarity).By similarity2 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule binding Source: BHF-UCL
  • protein C-terminus binding Source: ARUK-UCL
  • protein kinase binding Source: ARUK-UCL

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: BHF-UCL
  • positive regulation of microtubule plus-end binding Source: Ensembl
  • positive regulation of protein kinase activity Source: ARUK-UCL
  • positive regulation of transcription, DNA-templated Source: BHF-UCL
  • protein localization Source: ARUK-UCL

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

SIGNORiQ9UPY8

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein RP/EB family member 3
Alternative name(s):
EB1 protein family member 3
Short name:
EBF3
End-binding protein 3
Short name:
EB3
RP3
Gene namesi
Name:MAPRE3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000084764.10
HGNCiHGNC:6892 MAPRE3
MIMi605788 gene
neXtProtiNX_Q9UPY8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi226Y → A: Loss of localization of CAMSAP2 stretches to the Golgi apparatus; when associated with A-234. 1 Publication1
Mutagenesisi234E → A: Loss of localization of CAMSAP2 stretches to the Golgi apparatus; when associated with A-226. 1 Publication1

Organism-specific databases

DisGeNETi22924
OpenTargetsiENSG00000084764
PharmGKBiPA30636

Polymorphism and mutation databases

BioMutaiMAPRE3
DMDMi20138791

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002134281 – 281Microtubule-associated protein RP/EB family member 3Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineCombined sources1
Modified residuei176PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UPY8
PaxDbiQ9UPY8
PeptideAtlasiQ9UPY8
PRIDEiQ9UPY8
ProteomicsDBi85475
85476 [Q9UPY8-2]

PTM databases

iPTMnetiQ9UPY8
PhosphoSitePlusiQ9UPY8

Expressioni

Tissue specificityi

Predominantly expressed in brain and muscle.1 Publication

Gene expression databases

BgeeiENSG00000084764
CleanExiHS_MAPRE3
ExpressionAtlasiQ9UPY8 baseline and differential
GenevisibleiQ9UPY8 HS

Organism-specific databases

HPAiHPA009263

Interactioni

Subunit structurei

Interacts with APC2 (PubMed:10644998). Homodimer (PubMed:19255245). Heterodimer with MAPRE1 (PubMed:19255245). Interacts with DCTN1 and SRCIN1 (PubMed:14514668, PubMed:19146815). Binds to the C-terminal domain of APC (PubMed:14514668). Binds monomeric and polymerized tubulin (PubMed:10188731). Interacts (via C-terminus) with CLIP1 (PubMed:17563362). Interacts with SLAIN2 and SLAIN1 (PubMed:21646404). Interacts with AKAP9 (PubMed:28814570). Interacts with PDE4DIP (PubMed:28814570). Interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus (PubMed:25217626).9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule binding Source: BHF-UCL
  • protein C-terminus binding Source: ARUK-UCL
  • protein kinase binding Source: ARUK-UCL

Protein-protein interaction databases

BioGridi116584, 29 interactors
DIPiDIP-56069N
IntActiQ9UPY8, 53 interactors
MINTiQ9UPY8
STRINGi9606.ENSP00000233121

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 28Combined sources12
Helixi35 – 40Combined sources6
Helixi42 – 51Combined sources10
Beta strandi52 – 54Combined sources3
Helixi58 – 60Combined sources3
Helixi68 – 85Combined sources18
Helixi93 – 97Combined sources5
Helixi101 – 118Combined sources18
Turni126 – 129Combined sources4
Helixi206 – 238Combined sources33
Helixi247 – 255Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYONMR-A1-146[»]
3CO1X-ray1.40A1-130[»]
3JAKelectron microscopy3.50M/N1-200[»]
3JALelectron microscopy3.50M/N1-200[»]
3JARelectron microscopy3.50M/N1-200[»]
3TQ7X-ray2.30B200-281[»]
ProteinModelPortaliQ9UPY8
SMRiQ9UPY8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UPY8

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 116Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST103
Domaini194 – 264EB1 C-terminalPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 281DCTN1-bindingAdd BLAST65
Regioni217 – 260APC-bindingAdd BLAST44

Domaini

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.1 Publication

Sequence similaritiesi

Belongs to the MAPRE family.Curated

Phylogenomic databases

eggNOGiKOG3000 Eukaryota
COG5217 LUCA
GeneTreeiENSGT00490000043329
HOGENOMiHOG000198048
HOVERGENiHBG052410
InParanoidiQ9UPY8
KOiK10436
OMAiDSLHLTY
OrthoDBiEOG091G0FT4
PhylomeDBiQ9UPY8
TreeFamiTF313620

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR004953 EB1_C
IPR036133 EB1_C_sf
IPR027738 EB3
IPR027328 MAPRE
PANTHERiPTHR10623 PTHR10623, 1 hit
PTHR10623:SF10 PTHR10623:SF10, 1 hit
PfamiView protein in Pfam
PF00307 CH, 1 hit
PF03271 EB1, 1 hit
SUPFAMiSSF140612 SSF140612, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit
PS51230 EB1_C, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPY8-1) [UniParc]FASTAAdd to basket
Also known as: EBF3-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK
110 120 130 140 150
FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK
160 170 180 190 200
KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA
210 220 230 240 250
QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG
260 270 280
IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
Length:281
Mass (Da):31,982
Last modified:May 1, 2000 - v1
Checksum:i0DA45E89A0B993D3
GO
Isoform 2 (identifier: Q9UPY8-2) [UniParc]FASTAAdd to basket
Also known as: EBF3-S

The sequence of this isoform differs from the canonical sequence as follows:
     142-156: Missing.

Show »
Length:266
Mass (Da):30,380
Checksum:iE66CAD36E28EE645
GO

Sequence cautioni

The sequence AAK07556 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK07557 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA72060 differs from that shown. Reason: Frameshift at position 199.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → P in CAA72060 (PubMed:9233623).Curated1
Sequence conflicti13E → V in CAA72060 (PubMed:9233623).Curated1
Sequence conflicti123D → G in CAA72060 (PubMed:9233623).Curated1
Sequence conflicti237C → S in CAG38760 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012947142 – 156Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025186 mRNA Translation: BAA82958.1
AF288787 Genomic DNA Translation: AAK07556.1 Different initiation.
AF288787 Genomic DNA Translation: AAK07557.1 Different initiation.
CR536523 mRNA Translation: CAG38760.1
CR541845 mRNA Translation: CAG46643.1
AC013472 Genomic DNA Translation: AAY14653.1
CH471053 Genomic DNA Translation: EAX00660.1
CH471053 Genomic DNA Translation: EAX00662.1
CH471053 Genomic DNA Translation: EAX00663.1
CH471053 Genomic DNA Translation: EAX00666.1
BC011557 mRNA Translation: AAH11557.1
Y11174 mRNA Translation: CAA72060.1 Frameshift.
CCDSiCCDS1731.1 [Q9UPY8-1]
RefSeqiNP_001289979.1, NM_001303050.1 [Q9UPY8-1]
NP_036458.2, NM_012326.3 [Q9UPY8-1]
XP_006712030.1, XM_006711967.3 [Q9UPY8-2]
XP_011531002.1, XM_011532700.1 [Q9UPY8-2]
XP_016859086.1, XM_017003597.1 [Q9UPY8-2]
UniGeneiHs.515860

Genome annotation databases

EnsembliENST00000233121; ENSP00000233121; ENSG00000084764 [Q9UPY8-1]
ENST00000402218; ENSP00000385715; ENSG00000084764 [Q9UPY8-2]
ENST00000405074; ENSP00000383915; ENSG00000084764 [Q9UPY8-2]
GeneIDi22924
KEGGihsa:22924
UCSCiuc002rhw.4 human [Q9UPY8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMARE3_HUMAN
AccessioniPrimary (citable) accession number: Q9UPY8
Secondary accession number(s): B7WPK5
, O00265, Q6FHB0, Q6FI15, Q9BZP7, Q9BZP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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