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Protein

Microtubule-associated protein RP/EB family member 3

Gene

MAPRE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).By similarity1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000084764-MONOMER.
SIGNORiQ9UPY8.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein RP/EB family member 3
Alternative name(s):
EB1 protein family member 3
Short name:
EBF3
End-binding protein 3
Short name:
EB3
RP3
Gene namesi
Name:MAPRE3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:6892. MAPRE3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • microtubule cytoskeleton Source: BHF-UCL
  • microtubule plus-end Source: Ensembl
  • midbody Source: BHF-UCL
  • perinuclear region of cytoplasm Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi22924.
OpenTargetsiENSG00000084764.
PharmGKBiPA30636.

Polymorphism and mutation databases

BioMutaiMAPRE3.
DMDMi20138791.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002134282 – 281Microtubule-associated protein RP/EB family member 3Add BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei124PhosphotyrosineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei176PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UPY8.
PaxDbiQ9UPY8.
PeptideAtlasiQ9UPY8.
PRIDEiQ9UPY8.

PTM databases

iPTMnetiQ9UPY8.
PhosphoSitePlusiQ9UPY8.

Expressioni

Tissue specificityi

Predominantly expressed in brain and muscle.1 Publication

Gene expression databases

BgeeiENSG00000084764.
CleanExiHS_MAPRE3.
ExpressionAtlasiQ9UPY8. baseline and differential.
GenevisibleiQ9UPY8. HS.

Organism-specific databases

HPAiHPA009263.

Interactioni

Subunit structurei

Interacts with APC2 (By similarity). Homodimer. Heterodimer with MAPRE1. Interacts with DCTN1 and SRCIN1. Binds to the C-terminal domain of APC. Binds monomeric and polymerized tubulin. Interacts (via C-terminus) with CLIP1. Interacts with SLAIN2.By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-726739,EBI-726739
APC2O959967EBI-726739,EBI-1053045
APPL2Q8NEU83EBI-726739,EBI-741261
DCTN2Q135613EBI-726739,EBI-715074
EIF4E2O605733EBI-726739,EBI-398610
JUNP054123EBI-726739,EBI-852823
LMO2P257915EBI-726739,EBI-739696
MAPRE1Q1569114EBI-726739,EBI-1004115
MAPRE2Q155555EBI-726739,EBI-739717
MARCH7Q9H9924EBI-726739,EBI-949983
PSMA1P257864EBI-726739,EBI-359352
PSMB1P206183EBI-726739,EBI-372273
SDCBPO005607EBI-726739,EBI-727004
Srcin1Q9QWI6-25EBI-726739,EBI-775607From a different organism.
TROAPQ128156EBI-726739,EBI-2349743
TXN2Q997575EBI-726739,EBI-2932492

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi116584. 24 interactors.
DIPiDIP-56069N.
IntActiQ9UPY8. 33 interactors.
MINTiMINT-1417856.
STRINGi9606.ENSP00000233121.

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 28Combined sources12
Helixi35 – 40Combined sources6
Helixi42 – 51Combined sources10
Helixi58 – 60Combined sources3
Helixi68 – 85Combined sources18
Helixi93 – 97Combined sources5
Helixi101 – 118Combined sources18
Turni126 – 129Combined sources4
Helixi206 – 238Combined sources33
Helixi247 – 255Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYONMR-A1-146[»]
3CO1X-ray1.40A1-130[»]
3JAKelectron microscopy3.50M/N1-200[»]
3JALelectron microscopy3.50M/N1-200[»]
3JARelectron microscopy3.50M/N1-200[»]
3TQ7X-ray2.30B200-281[»]
ProteinModelPortaliQ9UPY8.
SMRiQ9UPY8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UPY8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 116CHPROSITE-ProRule annotationAdd BLAST103
Domaini194 – 264EB1 C-terminalPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 281DCTN1-bindingAdd BLAST65
Regioni217 – 260APC-bindingAdd BLAST44

Domaini

Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.

Sequence similaritiesi

Belongs to the MAPRE family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 EB1 C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3000. Eukaryota.
COG5217. LUCA.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
HOVERGENiHBG052410.
InParanoidiQ9UPY8.
KOiK10436.
OMAiNIQTSGR.
OrthoDBiEOG091G0FT4.
PhylomeDBiQ9UPY8.
TreeFamiTF313620.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027738. EB3_vertebrate.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PTHR10623:SF10. PTHR10623:SF10. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51230. EB1_C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPY8-1) [UniParc]FASTAAdd to basket
Also known as: EBF3-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM
60 70 80 90 100
LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK
110 120 130 140 150
FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK
160 170 180 190 200
KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA
210 220 230 240 250
QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG
260 270 280
IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
Length:281
Mass (Da):31,982
Last modified:May 1, 2000 - v1
Checksum:i0DA45E89A0B993D3
GO
Isoform 2 (identifier: Q9UPY8-2) [UniParc]FASTAAdd to basket
Also known as: EBF3-S

The sequence of this isoform differs from the canonical sequence as follows:
     142-156: Missing.

Show »
Length:266
Mass (Da):30,380
Checksum:iE66CAD36E28EE645
GO

Sequence cautioni

The sequence AAK07556 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK07557 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA72060 differs from that shown. Reason: Frameshift at position 199.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → P in CAA72060 (PubMed:9233623).Curated1
Sequence conflicti13E → V in CAA72060 (PubMed:9233623).Curated1
Sequence conflicti123D → G in CAA72060 (PubMed:9233623).Curated1
Sequence conflicti237C → S in CAG38760 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012947142 – 156Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025186 mRNA. Translation: BAA82958.1.
AF288787 Genomic DNA. Translation: AAK07556.1. Different initiation.
AF288787 Genomic DNA. Translation: AAK07557.1. Different initiation.
CR536523 mRNA. Translation: CAG38760.1.
CR541845 mRNA. Translation: CAG46643.1.
AC013472 Genomic DNA. Translation: AAY14653.1.
CH471053 Genomic DNA. Translation: EAX00660.1.
CH471053 Genomic DNA. Translation: EAX00662.1.
CH471053 Genomic DNA. Translation: EAX00663.1.
CH471053 Genomic DNA. Translation: EAX00666.1.
BC011557 mRNA. Translation: AAH11557.1.
Y11174 mRNA. Translation: CAA72060.1. Frameshift.
CCDSiCCDS1731.1. [Q9UPY8-1]
RefSeqiNP_001289979.1. NM_001303050.1. [Q9UPY8-1]
NP_036458.2. NM_012326.3. [Q9UPY8-1]
XP_006712030.1. XM_006711967.3. [Q9UPY8-2]
XP_011531002.1. XM_011532700.1. [Q9UPY8-2]
XP_016859086.1. XM_017003597.1. [Q9UPY8-2]
UniGeneiHs.515860.

Genome annotation databases

EnsembliENST00000233121; ENSP00000233121; ENSG00000084764. [Q9UPY8-1]
ENST00000402218; ENSP00000385715; ENSG00000084764. [Q9UPY8-2]
ENST00000405074; ENSP00000383915; ENSG00000084764. [Q9UPY8-2]
GeneIDi22924.
KEGGihsa:22924.
UCSCiuc002rhw.4. human. [Q9UPY8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025186 mRNA. Translation: BAA82958.1.
AF288787 Genomic DNA. Translation: AAK07556.1. Different initiation.
AF288787 Genomic DNA. Translation: AAK07557.1. Different initiation.
CR536523 mRNA. Translation: CAG38760.1.
CR541845 mRNA. Translation: CAG46643.1.
AC013472 Genomic DNA. Translation: AAY14653.1.
CH471053 Genomic DNA. Translation: EAX00660.1.
CH471053 Genomic DNA. Translation: EAX00662.1.
CH471053 Genomic DNA. Translation: EAX00663.1.
CH471053 Genomic DNA. Translation: EAX00666.1.
BC011557 mRNA. Translation: AAH11557.1.
Y11174 mRNA. Translation: CAA72060.1. Frameshift.
CCDSiCCDS1731.1. [Q9UPY8-1]
RefSeqiNP_001289979.1. NM_001303050.1. [Q9UPY8-1]
NP_036458.2. NM_012326.3. [Q9UPY8-1]
XP_006712030.1. XM_006711967.3. [Q9UPY8-2]
XP_011531002.1. XM_011532700.1. [Q9UPY8-2]
XP_016859086.1. XM_017003597.1. [Q9UPY8-2]
UniGeneiHs.515860.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYONMR-A1-146[»]
3CO1X-ray1.40A1-130[»]
3JAKelectron microscopy3.50M/N1-200[»]
3JALelectron microscopy3.50M/N1-200[»]
3JARelectron microscopy3.50M/N1-200[»]
3TQ7X-ray2.30B200-281[»]
ProteinModelPortaliQ9UPY8.
SMRiQ9UPY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116584. 24 interactors.
DIPiDIP-56069N.
IntActiQ9UPY8. 33 interactors.
MINTiMINT-1417856.
STRINGi9606.ENSP00000233121.

PTM databases

iPTMnetiQ9UPY8.
PhosphoSitePlusiQ9UPY8.

Polymorphism and mutation databases

BioMutaiMAPRE3.
DMDMi20138791.

Proteomic databases

EPDiQ9UPY8.
PaxDbiQ9UPY8.
PeptideAtlasiQ9UPY8.
PRIDEiQ9UPY8.

Protocols and materials databases

DNASUi22924.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000233121; ENSP00000233121; ENSG00000084764. [Q9UPY8-1]
ENST00000402218; ENSP00000385715; ENSG00000084764. [Q9UPY8-2]
ENST00000405074; ENSP00000383915; ENSG00000084764. [Q9UPY8-2]
GeneIDi22924.
KEGGihsa:22924.
UCSCiuc002rhw.4. human. [Q9UPY8-1]

Organism-specific databases

CTDi22924.
DisGeNETi22924.
GeneCardsiMAPRE3.
HGNCiHGNC:6892. MAPRE3.
HPAiHPA009263.
MIMi605788. gene.
neXtProtiNX_Q9UPY8.
OpenTargetsiENSG00000084764.
PharmGKBiPA30636.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3000. Eukaryota.
COG5217. LUCA.
GeneTreeiENSGT00490000043329.
HOGENOMiHOG000198048.
HOVERGENiHBG052410.
InParanoidiQ9UPY8.
KOiK10436.
OMAiNIQTSGR.
OrthoDBiEOG091G0FT4.
PhylomeDBiQ9UPY8.
TreeFamiTF313620.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000084764-MONOMER.
SIGNORiQ9UPY8.

Miscellaneous databases

ChiTaRSiMAPRE3. human.
EvolutionaryTraceiQ9UPY8.
GeneWikiiMAPRE3.
GenomeRNAii22924.
PROiQ9UPY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000084764.
CleanExiHS_MAPRE3.
ExpressionAtlasiQ9UPY8. baseline and differential.
GenevisibleiQ9UPY8. HS.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR004953. EB1_C.
IPR027738. EB3_vertebrate.
IPR027328. MAPRE.
[Graphical view]
PANTHERiPTHR10623. PTHR10623. 1 hit.
PTHR10623:SF10. PTHR10623:SF10. 1 hit.
PfamiPF00307. CH. 1 hit.
PF03271. EB1. 1 hit.
[Graphical view]
SUPFAMiSSF140612. SSF140612. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS51230. EB1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMARE3_HUMAN
AccessioniPrimary (citable) accession number: Q9UPY8
Secondary accession number(s): B7WPK5
, O00265, Q6FHB0, Q6FI15, Q9BZP7, Q9BZP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.