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Q9UPY6

- WASF3_HUMAN

UniProt

Q9UPY6 - WASF3_HUMAN

Protein

Wiskott-Aldrich syndrome protein family member 3

Gene

WASF3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.2 Publications

    GO - Biological processi

    1. actin filament polymerization Source: ProtInc
    2. cytoskeleton organization Source: UniProtKB
    3. lamellipodium assembly Source: Ensembl
    4. oligodendrocyte development Source: Ensembl
    5. positive regulation of myelination Source: Ensembl
    6. protein complex assembly Source: ProtInc
    7. regulation of cell shape Source: UniProtKB

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_160086. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Wiskott-Aldrich syndrome protein family member 3
    Short name:
    WASP family protein member 3
    Alternative name(s):
    Protein WAVE-3
    Verprolin homology domain-containing protein 3
    Gene namesi
    Name:WASF3
    Synonyms:KIAA0900, SCAR3, WAVE3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:12734. WASF3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. cytoskeleton Source: UniProtKB-SubCell
    3. lamellipodium Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37345.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Wiskott-Aldrich syndrome protein family member 3PRO_0000188996Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511Phosphotyrosine; by ABL11 Publication
    Modified residuei248 – 2481Phosphotyrosine; by ABL11 Publication
    Modified residuei337 – 3371Phosphotyrosine; by ABL11 Publication
    Modified residuei486 – 4861Phosphotyrosine; by ABL11 Publication

    Post-translational modificationi

    Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9UPY6.
    PaxDbiQ9UPY6.
    PRIDEiQ9UPY6.

    PTM databases

    PhosphoSiteiQ9UPY6.

    Miscellaneous databases

    PMAP-CutDBQ9UPY6.

    Expressioni

    Tissue specificityi

    Expressed in ovary and brain.

    Gene expression databases

    ArrayExpressiQ9UPY6.
    BgeeiQ9UPY6.
    CleanExiHS_WASF3.
    GenevestigatoriQ9UPY6.

    Organism-specific databases

    HPAiCAB037086.

    Interactioni

    Subunit structurei

    Binds actin and the Arp2/3 complex.

    Protein-protein interaction databases

    BioGridi116024. 4 interactions.
    IntActiQ9UPY6. 1 interaction.
    STRINGi9606.ENSP00000335055.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UPY6.
    SMRiQ9UPY6. Positions 17-200, 439-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini440 – 45718WH2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili57 – 9337Sequence AnalysisAdd
    BLAST
    Coiled coili162 – 20645Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi304 – 3107Poly-Pro
    Compositional biasi343 – 3508Poly-Pro
    Compositional biasi395 – 41016Poly-ProAdd
    BLAST

    Domaini

    Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

    Sequence similaritiesi

    Belongs to the SCAR/WAVE family.Curated
    Contains 1 WH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG299696.
    HOGENOMiHOG000021456.
    HOVERGENiHBG058482.
    InParanoidiQ9UPY6.
    KOiK06083.
    OMAiIERNTAM.
    PhylomeDBiQ9UPY6.
    TreeFamiTF315031.

    Family and domain databases

    InterProiIPR028288. SCAR/WAVE_fam.
    IPR003124. WH2_dom.
    [Graphical view]
    PANTHERiPTHR12902. PTHR12902. 1 hit.
    PfamiPF02205. WH2. 1 hit.
    [Graphical view]
    SMARTiSM00246. WH2. 1 hit.
    [Graphical view]
    PROSITEiPS51082. WH2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UPY6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPLVKRNIEP RHLCRGALPE GITSELECVT NSTLAAIIRQ LSSLSKHAED    50
    IFGELFNEAN NFYIRANSLQ DRIDRLAVKV TQLDSTVEEV SLQDINMKKA 100
    FKSSTVQDQQ VVSKNSIPNP VADIYNQSDK PPPLNILTPY RDDKKDGLKF 150
    YTDPSYFFDL WKEKMLQDTE DKRKEKRRQK EQKRIDGTTR EVKKVRKARN 200
    RRQEWNMMAY DKELRPDNRL SQSVYHGASS EGSLSPDTRS HASDVTDYSY 250
    PATPNHSLHP QPVTPSYAAG DVPPHGPASQ AAEHEYRPPS ASARHMALNR 300
    PQQPPPPPPP QAPEGSQASA PMAPADYGML PAQIIEYYNP SGPPPPPPPP 350
    VIPSAQTAFV SPLQMPMQPP FPASASSTHA APPHPPSTGL LVTAPPPPGP 400
    PPPPPGPPGP GSSLSSSPMH GPPVAEAKRQ EPAQPPISDA RSDLLAAIRM 450
    GIQLKKVQEQ REQEAKREPV GNDVATILSR RIAVEYSDSD DDSEFDENDW 500
    SD 502
    Length:502
    Mass (Da):55,293
    Last modified:February 1, 2005 - v2
    Checksum:iC0655CE181BD3C57
    GO
    Isoform 2 (identifier: Q9UPY6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         181-239: EQKRIDGTTR...SEGSLSPDTR → REKHKLNPNR...SAKEDRVPSG

    Note: No experimental confirmation available.

    Show »
    Length:499
    Mass (Da):55,007
    Checksum:iD32CA1466AF9B1CE
    GO

    Sequence cautioni

    The sequence BAA74923.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti307 – 3082PP → RR(PubMed:10381382)Curated
    Sequence conflicti307 – 3082PP → RR(PubMed:12856283)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti415 – 4151S → L.
    Corresponds to variant rs17084492 [ dbSNP | Ensembl ].
    VAR_052953

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei181 – 23959EQKRI…SPDTR → REKHKLNPNRNQQVNVRKVR TRKEEWERRKMGIEFMSDAK KLEQAGSAKEDRVPSG in isoform 2. 1 PublicationVSP_054517Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026543 mRNA. Translation: BAA81796.1.
    AF454702 mRNA. Translation: AAL51032.1.
    AB020707 mRNA. Translation: BAA74923.2. Different initiation.
    AL163538, AL159978 Genomic DNA. Translation: CAH72487.1.
    AL353789 Genomic DNA. No translation available.
    CH471075 Genomic DNA. Translation: EAX08388.1.
    BC050283 mRNA. Translation: AAH50283.1.
    AF134305 mRNA. Translation: AAD33054.1.
    CCDSiCCDS9318.1. [Q9UPY6-1]
    RefSeqiNP_006637.2. NM_006646.5. [Q9UPY6-1]
    XP_005266296.1. XM_005266239.1. [Q9UPY6-2]
    UniGeneiHs.618732.

    Genome annotation databases

    EnsembliENST00000335327; ENSP00000335055; ENSG00000132970. [Q9UPY6-1]
    ENST00000361042; ENSP00000354325; ENSG00000132970. [Q9UPY6-2]
    GeneIDi10810.
    KEGGihsa:10810.
    UCSCiuc001uqv.3. human. [Q9UPY6-1]
    uc001uqw.3. human.

    Polymorphism databases

    DMDMi59800455.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB026543 mRNA. Translation: BAA81796.1 .
    AF454702 mRNA. Translation: AAL51032.1 .
    AB020707 mRNA. Translation: BAA74923.2 . Different initiation.
    AL163538 , AL159978 Genomic DNA. Translation: CAH72487.1 .
    AL353789 Genomic DNA. No translation available.
    CH471075 Genomic DNA. Translation: EAX08388.1 .
    BC050283 mRNA. Translation: AAH50283.1 .
    AF134305 mRNA. Translation: AAD33054.1 .
    CCDSi CCDS9318.1. [Q9UPY6-1 ]
    RefSeqi NP_006637.2. NM_006646.5. [Q9UPY6-1 ]
    XP_005266296.1. XM_005266239.1. [Q9UPY6-2 ]
    UniGenei Hs.618732.

    3D structure databases

    ProteinModelPortali Q9UPY6.
    SMRi Q9UPY6. Positions 17-200, 439-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116024. 4 interactions.
    IntActi Q9UPY6. 1 interaction.
    STRINGi 9606.ENSP00000335055.

    PTM databases

    PhosphoSitei Q9UPY6.

    Polymorphism databases

    DMDMi 59800455.

    Proteomic databases

    MaxQBi Q9UPY6.
    PaxDbi Q9UPY6.
    PRIDEi Q9UPY6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000335327 ; ENSP00000335055 ; ENSG00000132970 . [Q9UPY6-1 ]
    ENST00000361042 ; ENSP00000354325 ; ENSG00000132970 . [Q9UPY6-2 ]
    GeneIDi 10810.
    KEGGi hsa:10810.
    UCSCi uc001uqv.3. human. [Q9UPY6-1 ]
    uc001uqw.3. human.

    Organism-specific databases

    CTDi 10810.
    GeneCardsi GC13P027132.
    HGNCi HGNC:12734. WASF3.
    HPAi CAB037086.
    MIMi 605068. gene.
    neXtProti NX_Q9UPY6.
    PharmGKBi PA37345.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG299696.
    HOGENOMi HOG000021456.
    HOVERGENi HBG058482.
    InParanoidi Q9UPY6.
    KOi K06083.
    OMAi IERNTAM.
    PhylomeDBi Q9UPY6.
    TreeFami TF315031.

    Enzyme and pathway databases

    Reactomei REACT_160086. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    ChiTaRSi WASF3. human.
    GeneWikii WASF3.
    GenomeRNAii 10810.
    NextBioi 41067.
    PMAP-CutDB Q9UPY6.
    PROi Q9UPY6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UPY6.
    Bgeei Q9UPY6.
    CleanExi HS_WASF3.
    Genevestigatori Q9UPY6.

    Family and domain databases

    InterProi IPR028288. SCAR/WAVE_fam.
    IPR003124. WH2_dom.
    [Graphical view ]
    PANTHERi PTHR12902. PTHR12902. 1 hit.
    Pfami PF02205. WH2. 1 hit.
    [Graphical view ]
    SMARTi SM00246. WH2. 1 hit.
    [Graphical view ]
    PROSITEi PS51082. WH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex."
      Suetsugu S., Miki H., Takenawa T.
      Biochem. Biophys. Res. Commun. 260:296-302(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "Genomic organization and expression profile of the human and mouse WAVE gene family."
      Sossey-Alaoui K., Head K., Nowak N., Cowell J.K.
      Mamm. Genome 14:314-322(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    5. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    8. Machesky L.M., Insall R.H.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-502 (ISOFORM 1).
    9. "c-Abl-mediated phosphorylation of WAVE3 is required for lamellipodia formation and cell migration."
      Sossey-Alaoui K., Li X., Cowell J.K.
      J. Biol. Chem. 282:26257-26265(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT TYR-151; TYR-248; TYR-337 AND TYR-486.
    10. "Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration."
      Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V., Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J.
      BMC Biol. 9:54-54(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiWASF3_HUMAN
    AccessioniPrimary (citable) accession number: Q9UPY6
    Secondary accession number(s): O94974, Q86VQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3