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Q9UPY6 (WASF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wiskott-Aldrich syndrome protein family member 3

Short name=WASP family protein member 3
Alternative name(s):
Protein WAVE-3
Verprolin homology domain-containing protein 3
Gene names
Name:WASF3
Synonyms:KIAA0900, SCAR3, WAVE3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Ref.7 Ref.8

Subunit structure

Binds actin and the Arp2/3 complex.

Subcellular location

Cytoplasmcytoskeleton.

Tissue specificity

Expressed in ovary and brain.

Domain

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Post-translational modification

Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.

Sequence similarities

Belongs to the SCAR/WAVE family.

Contains 1 WH2 domain.

Sequence caution

The sequence BAA74923.2 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Wiskott-Aldrich syndrome protein family member 3
PRO_0000188996

Regions

Domain440 – 45718WH2
Coiled coil57 – 9337 Potential
Coiled coil162 – 20645 Potential
Compositional bias304 – 3107Poly-Pro
Compositional bias343 – 3508Poly-Pro
Compositional bias395 – 41016Poly-Pro

Amino acid modifications

Modified residue1511Phosphotyrosine; by ABL1 Ref.7
Modified residue2481Phosphotyrosine; by ABL1 Ref.7
Modified residue3371Phosphotyrosine; by ABL1 Ref.7
Modified residue4861Phosphotyrosine; by ABL1 Ref.7

Natural variations

Natural variant4151S → L.
Corresponds to variant rs17084492 [ dbSNP | Ensembl ].
VAR_052953

Experimental info

Sequence conflict307 – 3082PP → RR Ref.1
Sequence conflict307 – 3082PP → RR Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9UPY6 [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: C0655CE181BD3C57

FASTA50255,293
        10         20         30         40         50         60 
MPLVKRNIEP RHLCRGALPE GITSELECVT NSTLAAIIRQ LSSLSKHAED IFGELFNEAN 

        70         80         90        100        110        120 
NFYIRANSLQ DRIDRLAVKV TQLDSTVEEV SLQDINMKKA FKSSTVQDQQ VVSKNSIPNP 

       130        140        150        160        170        180 
VADIYNQSDK PPPLNILTPY RDDKKDGLKF YTDPSYFFDL WKEKMLQDTE DKRKEKRRQK 

       190        200        210        220        230        240 
EQKRIDGTTR EVKKVRKARN RRQEWNMMAY DKELRPDNRL SQSVYHGASS EGSLSPDTRS 

       250        260        270        280        290        300 
HASDVTDYSY PATPNHSLHP QPVTPSYAAG DVPPHGPASQ AAEHEYRPPS ASARHMALNR 

       310        320        330        340        350        360 
PQQPPPPPPP QAPEGSQASA PMAPADYGML PAQIIEYYNP SGPPPPPPPP VIPSAQTAFV 

       370        380        390        400        410        420 
SPLQMPMQPP FPASASSTHA APPHPPSTGL LVTAPPPPGP PPPPPGPPGP GSSLSSSPMH 

       430        440        450        460        470        480 
GPPVAEAKRQ EPAQPPISDA RSDLLAAIRM GIQLKKVQEQ REQEAKREPV GNDVATILSR 

       490        500 
RIAVEYSDSD DDSEFDENDW SD 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex."
Suetsugu S., Miki H., Takenawa T.
Biochem. Biophys. Res. Commun. 260:296-302(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Genomic organization and expression profile of the human and mouse WAVE gene family."
Sossey-Alaoui K., Head K., Nowak N., Cowell J.K.
Mamm. Genome 14:314-322(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[5]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Machesky L.M., Insall R.H.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-502.
[7]"c-Abl-mediated phosphorylation of WAVE3 is required for lamellipodia formation and cell migration."
Sossey-Alaoui K., Li X., Cowell J.K.
J. Biol. Chem. 282:26257-26265(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT TYR-151; TYR-248; TYR-337 AND TYR-486.
[8]"Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration."
Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V., Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J.
BMC Biol. 9:54-54(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026543 mRNA. Translation: BAA81796.1.
AF454702 mRNA. Translation: AAL51032.1.
AB020707 mRNA. Translation: BAA74923.2. Different initiation.
AL163538, AL159978 Genomic DNA. Translation: CAH72487.1.
AF134305 mRNA. Translation: AAD33054.1.
RefSeqNP_006637.2. NM_006646.5.
UniGeneHs.618732.

3D structure databases

ProteinModelPortalQ9UPY6.
SMRQ9UPY6. Positions 21-208, 439-487.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116024. 4 interactions.
IntActQ9UPY6. 1 interaction.
STRING9606.ENSP00000335055.

PTM databases

PhosphoSiteQ9UPY6.

Polymorphism databases

DMDM59800455.

Proteomic databases

PaxDbQ9UPY6.
PRIDEQ9UPY6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000335327; ENSP00000335055; ENSG00000132970.
GeneID10810.
KEGGhsa:10810.
UCSCuc001uqv.3. human.

Organism-specific databases

CTD10810.
GeneCardsGC13P027132.
HGNCHGNC:12734. WASF3.
HPACAB037086.
MIM605068. gene.
neXtProtNX_Q9UPY6.
PharmGKBPA37345.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG299696.
HOGENOMHOG000021456.
HOVERGENHBG058482.
InParanoidQ9UPY6.
KOK06083.
OMAIERNTAM.
PhylomeDBQ9UPY6.
TreeFamTF315031.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ9UPY6.
BgeeQ9UPY6.
CleanExHS_WASF3.
GenevestigatorQ9UPY6.

Family and domain databases

InterProIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERPTHR12902. PTHR12902. 1 hit.
PfamPF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSWASF3. human.
GeneWikiWASF3.
GenomeRNAi10810.
NextBio41067.
PMAP-CutDBQ9UPY6.
PROQ9UPY6.
SOURCESearch...

Entry information

Entry nameWASF3_HUMAN
AccessionPrimary (citable) accession number: Q9UPY6
Secondary accession number(s): O94974
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM