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Q9UPY6

- WASF3_HUMAN

UniProt

Q9UPY6 - WASF3_HUMAN

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Protein

Wiskott-Aldrich syndrome protein family member 3

Gene

WASF3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.2 Publications

GO - Biological processi

  1. actin filament polymerization Source: ProtInc
  2. cytoskeleton organization Source: UniProtKB
  3. lamellipodium assembly Source: Ensembl
  4. oligodendrocyte development Source: Ensembl
  5. positive regulation of myelination Source: Ensembl
  6. protein complex assembly Source: ProtInc
  7. regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_160086. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 3
Short name:
WASP family protein member 3
Alternative name(s):
Protein WAVE-3
Verprolin homology domain-containing protein 3
Gene namesi
Name:WASF3
Synonyms:KIAA0900, SCAR3, WAVE3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 13

Organism-specific databases

HGNCiHGNC:12734. WASF3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: UniProtKB
  4. lamellipodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37345.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Wiskott-Aldrich syndrome protein family member 3PRO_0000188996Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511Phosphotyrosine; by ABL11 Publication
Modified residuei248 – 2481Phosphotyrosine; by ABL11 Publication
Modified residuei337 – 3371Phosphotyrosine; by ABL11 Publication
Modified residuei486 – 4861Phosphotyrosine; by ABL11 Publication

Post-translational modificationi

Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UPY6.
PaxDbiQ9UPY6.
PRIDEiQ9UPY6.

PTM databases

PhosphoSiteiQ9UPY6.

Miscellaneous databases

PMAP-CutDBQ9UPY6.

Expressioni

Tissue specificityi

Expressed in ovary and brain.

Gene expression databases

BgeeiQ9UPY6.
CleanExiHS_WASF3.
ExpressionAtlasiQ9UPY6. baseline and differential.
GenevestigatoriQ9UPY6.

Organism-specific databases

HPAiCAB037086.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex.

Protein-protein interaction databases

BioGridi116024. 5 interactions.
IntActiQ9UPY6. 2 interactions.
STRINGi9606.ENSP00000335055.

Structurei

3D structure databases

ProteinModelPortaliQ9UPY6.
SMRiQ9UPY6. Positions 17-200, 439-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini440 – 45718WH2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili57 – 9337Sequence AnalysisAdd
BLAST
Coiled coili162 – 20645Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi304 – 3107Poly-Pro
Compositional biasi343 – 3508Poly-Pro
Compositional biasi395 – 41016Poly-ProAdd
BLAST

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG299696.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ9UPY6.
KOiK06083.
OMAiIERNTAM.
PhylomeDBiQ9UPY6.
TreeFamiTF315031.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UPY6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLVKRNIEP RHLCRGALPE GITSELECVT NSTLAAIIRQ LSSLSKHAED
60 70 80 90 100
IFGELFNEAN NFYIRANSLQ DRIDRLAVKV TQLDSTVEEV SLQDINMKKA
110 120 130 140 150
FKSSTVQDQQ VVSKNSIPNP VADIYNQSDK PPPLNILTPY RDDKKDGLKF
160 170 180 190 200
YTDPSYFFDL WKEKMLQDTE DKRKEKRRQK EQKRIDGTTR EVKKVRKARN
210 220 230 240 250
RRQEWNMMAY DKELRPDNRL SQSVYHGASS EGSLSPDTRS HASDVTDYSY
260 270 280 290 300
PATPNHSLHP QPVTPSYAAG DVPPHGPASQ AAEHEYRPPS ASARHMALNR
310 320 330 340 350
PQQPPPPPPP QAPEGSQASA PMAPADYGML PAQIIEYYNP SGPPPPPPPP
360 370 380 390 400
VIPSAQTAFV SPLQMPMQPP FPASASSTHA APPHPPSTGL LVTAPPPPGP
410 420 430 440 450
PPPPPGPPGP GSSLSSSPMH GPPVAEAKRQ EPAQPPISDA RSDLLAAIRM
460 470 480 490 500
GIQLKKVQEQ REQEAKREPV GNDVATILSR RIAVEYSDSD DDSEFDENDW

SD
Length:502
Mass (Da):55,293
Last modified:February 1, 2005 - v2
Checksum:iC0655CE181BD3C57
GO
Isoform 2 (identifier: Q9UPY6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-239: EQKRIDGTTR...SEGSLSPDTR → REKHKLNPNR...SAKEDRVPSG

Note: No experimental confirmation available.

Show »
Length:499
Mass (Da):55,007
Checksum:iD32CA1466AF9B1CE
GO

Sequence cautioni

The sequence BAA74923.2 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti307 – 3082PP → RR(PubMed:10381382)Curated
Sequence conflicti307 – 3082PP → RR(PubMed:12856283)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti415 – 4151S → L.
Corresponds to variant rs17084492 [ dbSNP | Ensembl ].
VAR_052953

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei181 – 23959EQKRI…SPDTR → REKHKLNPNRNQQVNVRKVR TRKEEWERRKMGIEFMSDAK KLEQAGSAKEDRVPSG in isoform 2. 1 PublicationVSP_054517Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026543 mRNA. Translation: BAA81796.1.
AF454702 mRNA. Translation: AAL51032.1.
AB020707 mRNA. Translation: BAA74923.2. Different initiation.
AL163538, AL159978 Genomic DNA. Translation: CAH72487.1.
AL353789 Genomic DNA. No translation available.
CH471075 Genomic DNA. Translation: EAX08388.1.
BC050283 mRNA. Translation: AAH50283.1.
AF134305 mRNA. Translation: AAD33054.1.
CCDSiCCDS9318.1. [Q9UPY6-1]
RefSeqiNP_001278894.1. NM_001291965.1. [Q9UPY6-2]
NP_006637.2. NM_006646.5. [Q9UPY6-1]
XP_005266296.1. XM_005266239.1. [Q9UPY6-2]
UniGeneiHs.618732.

Genome annotation databases

EnsembliENST00000335327; ENSP00000335055; ENSG00000132970. [Q9UPY6-1]
ENST00000361042; ENSP00000354325; ENSG00000132970. [Q9UPY6-2]
GeneIDi10810.
KEGGihsa:10810.
UCSCiuc001uqv.3. human. [Q9UPY6-1]
uc001uqw.3. human.

Polymorphism databases

DMDMi59800455.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB026543 mRNA. Translation: BAA81796.1 .
AF454702 mRNA. Translation: AAL51032.1 .
AB020707 mRNA. Translation: BAA74923.2 . Different initiation.
AL163538 , AL159978 Genomic DNA. Translation: CAH72487.1 .
AL353789 Genomic DNA. No translation available.
CH471075 Genomic DNA. Translation: EAX08388.1 .
BC050283 mRNA. Translation: AAH50283.1 .
AF134305 mRNA. Translation: AAD33054.1 .
CCDSi CCDS9318.1. [Q9UPY6-1 ]
RefSeqi NP_001278894.1. NM_001291965.1. [Q9UPY6-2 ]
NP_006637.2. NM_006646.5. [Q9UPY6-1 ]
XP_005266296.1. XM_005266239.1. [Q9UPY6-2 ]
UniGenei Hs.618732.

3D structure databases

ProteinModelPortali Q9UPY6.
SMRi Q9UPY6. Positions 17-200, 439-487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116024. 5 interactions.
IntActi Q9UPY6. 2 interactions.
STRINGi 9606.ENSP00000335055.

PTM databases

PhosphoSitei Q9UPY6.

Polymorphism databases

DMDMi 59800455.

Proteomic databases

MaxQBi Q9UPY6.
PaxDbi Q9UPY6.
PRIDEi Q9UPY6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000335327 ; ENSP00000335055 ; ENSG00000132970 . [Q9UPY6-1 ]
ENST00000361042 ; ENSP00000354325 ; ENSG00000132970 . [Q9UPY6-2 ]
GeneIDi 10810.
KEGGi hsa:10810.
UCSCi uc001uqv.3. human. [Q9UPY6-1 ]
uc001uqw.3. human.

Organism-specific databases

CTDi 10810.
GeneCardsi GC13P027132.
HGNCi HGNC:12734. WASF3.
HPAi CAB037086.
MIMi 605068. gene.
neXtProti NX_Q9UPY6.
PharmGKBi PA37345.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG299696.
GeneTreei ENSGT00730000110593.
HOGENOMi HOG000021456.
HOVERGENi HBG058482.
InParanoidi Q9UPY6.
KOi K06083.
OMAi IERNTAM.
PhylomeDBi Q9UPY6.
TreeFami TF315031.

Enzyme and pathway databases

Reactomei REACT_160086. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

ChiTaRSi WASF3. human.
GeneWikii WASF3.
GenomeRNAii 10810.
NextBioi 41067.
PMAP-CutDB Q9UPY6.
PROi Q9UPY6.
SOURCEi Search...

Gene expression databases

Bgeei Q9UPY6.
CleanExi HS_WASF3.
ExpressionAtlasi Q9UPY6. baseline and differential.
Genevestigatori Q9UPY6.

Family and domain databases

InterProi IPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view ]
PANTHERi PTHR12902. PTHR12902. 1 hit.
Pfami PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00246. WH2. 1 hit.
[Graphical view ]
PROSITEi PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex."
    Suetsugu S., Miki H., Takenawa T.
    Biochem. Biophys. Res. Commun. 260:296-302(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Genomic organization and expression profile of the human and mouse WAVE gene family."
    Sossey-Alaoui K., Head K., Nowak N., Cowell J.K.
    Mamm. Genome 14:314-322(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
    Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
    DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  5. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  8. Machesky L.M., Insall R.H.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-502 (ISOFORM 1).
  9. "c-Abl-mediated phosphorylation of WAVE3 is required for lamellipodia formation and cell migration."
    Sossey-Alaoui K., Li X., Cowell J.K.
    J. Biol. Chem. 282:26257-26265(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT TYR-151; TYR-248; TYR-337 AND TYR-486.
  10. "Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration."
    Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V., Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J.
    BMC Biol. 9:54-54(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiWASF3_HUMAN
AccessioniPrimary (citable) accession number: Q9UPY6
Secondary accession number(s): O94974, Q86VQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 2005
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3