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Protein

Wiskott-Aldrich syndrome protein family member 3

Gene

WASF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.2 Publications

GO - Biological processi

  • actin filament polymerization Source: ProtInc
  • cytoskeleton organization Source: UniProtKB
  • lamellipodium assembly Source: Ensembl
  • oligodendrocyte development Source: Ensembl
  • positive regulation of myelination Source: Ensembl
  • protein complex assembly Source: ProtInc
  • regulation of cell shape Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132970-MONOMER.
ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 3
Short name:
WASP family protein member 3
Alternative name(s):
Protein WAVE-3
Verprolin homology domain-containing protein 3
Gene namesi
Name:WASF3
Synonyms:KIAA0900, SCAR3, WAVE3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:12734. WASF3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • lamellipodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi10810.
OpenTargetsiENSG00000132970.
PharmGKBiPA37345.

Polymorphism and mutation databases

BioMutaiWASF3.
DMDMi59800455.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889961 – 502Wiskott-Aldrich syndrome protein family member 3Add BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151Phosphotyrosine; by ABL11 Publication1
Modified residuei248Phosphotyrosine; by ABL11 Publication1
Modified residuei337Phosphotyrosine; by ABL11 Publication1
Modified residuei486Phosphotyrosine; by ABL11 Publication1

Post-translational modificationi

Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UPY6.
PaxDbiQ9UPY6.
PeptideAtlasiQ9UPY6.
PRIDEiQ9UPY6.

PTM databases

iPTMnetiQ9UPY6.
PhosphoSitePlusiQ9UPY6.

Miscellaneous databases

PMAP-CutDBQ9UPY6.

Expressioni

Tissue specificityi

Expressed in ovary and brain.

Gene expression databases

BgeeiENSG00000132970.
CleanExiHS_WASF3.
ExpressionAtlasiQ9UPY6. baseline and differential.
GenevisibleiQ9UPY6. HS.

Organism-specific databases

HPAiCAB037086.
HPA056574.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex.

Protein-protein interaction databases

BioGridi116024. 24 interactors.
DIPiDIP-61425N.
IntActiQ9UPY6. 8 interactors.
STRINGi9606.ENSP00000335055.

Structurei

3D structure databases

ProteinModelPortaliQ9UPY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini440 – 457WH2PROSITE-ProRule annotationAdd BLAST18

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili57 – 93Sequence analysisAdd BLAST37
Coiled coili162 – 206Sequence analysisAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi304 – 310Poly-Pro7
Compositional biasi343 – 350Poly-Pro8
Compositional biasi395 – 410Poly-ProAdd BLAST16

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1830. Eukaryota.
ENOG410XRBQ. LUCA.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ9UPY6.
KOiK06083.
OMAiDQHHYPN.
OrthoDBiEOG091G08O1.
PhylomeDBiQ9UPY6.
TreeFamiTF315031.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPY6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLVKRNIEP RHLCRGALPE GITSELECVT NSTLAAIIRQ LSSLSKHAED
60 70 80 90 100
IFGELFNEAN NFYIRANSLQ DRIDRLAVKV TQLDSTVEEV SLQDINMKKA
110 120 130 140 150
FKSSTVQDQQ VVSKNSIPNP VADIYNQSDK PPPLNILTPY RDDKKDGLKF
160 170 180 190 200
YTDPSYFFDL WKEKMLQDTE DKRKEKRRQK EQKRIDGTTR EVKKVRKARN
210 220 230 240 250
RRQEWNMMAY DKELRPDNRL SQSVYHGASS EGSLSPDTRS HASDVTDYSY
260 270 280 290 300
PATPNHSLHP QPVTPSYAAG DVPPHGPASQ AAEHEYRPPS ASARHMALNR
310 320 330 340 350
PQQPPPPPPP QAPEGSQASA PMAPADYGML PAQIIEYYNP SGPPPPPPPP
360 370 380 390 400
VIPSAQTAFV SPLQMPMQPP FPASASSTHA APPHPPSTGL LVTAPPPPGP
410 420 430 440 450
PPPPPGPPGP GSSLSSSPMH GPPVAEAKRQ EPAQPPISDA RSDLLAAIRM
460 470 480 490 500
GIQLKKVQEQ REQEAKREPV GNDVATILSR RIAVEYSDSD DDSEFDENDW

SD
Length:502
Mass (Da):55,293
Last modified:February 1, 2005 - v2
Checksum:iC0655CE181BD3C57
GO
Isoform 2 (identifier: Q9UPY6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-239: EQKRIDGTTR...SEGSLSPDTR → REKHKLNPNR...SAKEDRVPSG

Note: No experimental confirmation available.
Show »
Length:499
Mass (Da):55,007
Checksum:iD32CA1466AF9B1CE
GO

Sequence cautioni

The sequence BAA74923 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti307 – 308PP → RR (PubMed:10381382).Curated2
Sequence conflicti307 – 308PP → RR (PubMed:12856283).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052953415S → L.Corresponds to variant rs17084492dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054517181 – 239EQKRI…SPDTR → REKHKLNPNRNQQVNVRKVR TRKEEWERRKMGIEFMSDAK KLEQAGSAKEDRVPSG in isoform 2. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026543 mRNA. Translation: BAA81796.1.
AF454702 mRNA. Translation: AAL51032.1.
AB020707 mRNA. Translation: BAA74923.2. Different initiation.
AL163538, AL159978 Genomic DNA. Translation: CAH72487.1.
AL353789 Genomic DNA. No translation available.
CH471075 Genomic DNA. Translation: EAX08388.1.
BC050283 mRNA. Translation: AAH50283.1.
AF134305 mRNA. Translation: AAD33054.1.
CCDSiCCDS76626.1. [Q9UPY6-2]
CCDS9318.1. [Q9UPY6-1]
RefSeqiNP_001278894.1. NM_001291965.1. [Q9UPY6-2]
NP_006637.2. NM_006646.5. [Q9UPY6-1]
XP_011533191.1. XM_011534889.1. [Q9UPY6-1]
XP_011533192.1. XM_011534890.1. [Q9UPY6-1]
UniGeneiHs.618732.

Genome annotation databases

EnsembliENST00000335327; ENSP00000335055; ENSG00000132970. [Q9UPY6-1]
ENST00000361042; ENSP00000354325; ENSG00000132970. [Q9UPY6-2]
GeneIDi10810.
KEGGihsa:10810.
UCSCiuc001uqv.5. human. [Q9UPY6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026543 mRNA. Translation: BAA81796.1.
AF454702 mRNA. Translation: AAL51032.1.
AB020707 mRNA. Translation: BAA74923.2. Different initiation.
AL163538, AL159978 Genomic DNA. Translation: CAH72487.1.
AL353789 Genomic DNA. No translation available.
CH471075 Genomic DNA. Translation: EAX08388.1.
BC050283 mRNA. Translation: AAH50283.1.
AF134305 mRNA. Translation: AAD33054.1.
CCDSiCCDS76626.1. [Q9UPY6-2]
CCDS9318.1. [Q9UPY6-1]
RefSeqiNP_001278894.1. NM_001291965.1. [Q9UPY6-2]
NP_006637.2. NM_006646.5. [Q9UPY6-1]
XP_011533191.1. XM_011534889.1. [Q9UPY6-1]
XP_011533192.1. XM_011534890.1. [Q9UPY6-1]
UniGeneiHs.618732.

3D structure databases

ProteinModelPortaliQ9UPY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116024. 24 interactors.
DIPiDIP-61425N.
IntActiQ9UPY6. 8 interactors.
STRINGi9606.ENSP00000335055.

PTM databases

iPTMnetiQ9UPY6.
PhosphoSitePlusiQ9UPY6.

Polymorphism and mutation databases

BioMutaiWASF3.
DMDMi59800455.

Proteomic databases

EPDiQ9UPY6.
PaxDbiQ9UPY6.
PeptideAtlasiQ9UPY6.
PRIDEiQ9UPY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335327; ENSP00000335055; ENSG00000132970. [Q9UPY6-1]
ENST00000361042; ENSP00000354325; ENSG00000132970. [Q9UPY6-2]
GeneIDi10810.
KEGGihsa:10810.
UCSCiuc001uqv.5. human. [Q9UPY6-1]

Organism-specific databases

CTDi10810.
DisGeNETi10810.
GeneCardsiWASF3.
HGNCiHGNC:12734. WASF3.
HPAiCAB037086.
HPA056574.
MIMi605068. gene.
neXtProtiNX_Q9UPY6.
OpenTargetsiENSG00000132970.
PharmGKBiPA37345.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1830. Eukaryota.
ENOG410XRBQ. LUCA.
GeneTreeiENSGT00730000110593.
HOGENOMiHOG000021456.
HOVERGENiHBG058482.
InParanoidiQ9UPY6.
KOiK06083.
OMAiDQHHYPN.
OrthoDBiEOG091G08O1.
PhylomeDBiQ9UPY6.
TreeFamiTF315031.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132970-MONOMER.
ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-4420097. VEGFA-VEGFR2 Pathway.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

ChiTaRSiWASF3. human.
GeneWikiiWASF3.
GenomeRNAii10810.
PMAP-CutDBQ9UPY6.
PROiQ9UPY6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132970.
CleanExiHS_WASF3.
ExpressionAtlasiQ9UPY6. baseline and differential.
GenevisibleiQ9UPY6. HS.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASF3_HUMAN
AccessioniPrimary (citable) accession number: Q9UPY6
Secondary accession number(s): O94974, Q86VQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.