Q9UPY6 (WASF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wiskott-Aldrich syndrome protein family member 3
Short name=WASP family protein member 3

Alternative name(s):
Protein WAVE-3
Verprolin homology domain-containing protein 3

Gene names

Name:	WASF3
Synonyms:	KIAA0900, SCAR3, WAVE3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Ref.7 Ref.8
Subunit structure	Binds actin and the Arp2/3 complex.
Subcellular location	Cytoplasm › cytoskeleton.
Tissue specificity	Expressed in ovary and brain.
Domain	Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Post-translational modification	Phosphorylation by ABL1 promotes lamellipodia formation and cell migration.
Sequence similarities	Belongs to the SCAR/WAVE family. Contains 1 WH2 domain.
Sequence caution	The sequence BAA74923.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament polymerization Traceable author statement Ref.1. Source: ProtInc lamellipodium assembly Inferred from electronic annotation. Source: Compara oligodendrocyte development Inferred from electronic annotation. Source: Compara positive regulation of myelination Inferred from electronic annotation. Source: Compara regulation of cell shape Inferred from mutant phenotype Ref.8. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell lamellipodium Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Wiskott-Aldrich syndrome protein family member 3		PRO_0000188996
Regions
	Domain	440 – 457	18	WH2
	Coiled coil	57 – 93	37	Potential
	Coiled coil	162 – 206	45	Potential
	Compositional bias	304 – 310	7	Poly-Pro
	Compositional bias	343 – 350	8	Poly-Pro
	Compositional bias	395 – 410	16	Poly-Pro
Amino acid modifications
	Modified residue	151	1	Phosphotyrosine; by ABL1 Ref.7
	Modified residue	248	1	Phosphotyrosine; by ABL1 Ref.7
	Modified residue	337	1	Phosphotyrosine; by ABL1 Ref.7
	Modified residue	486	1	Phosphotyrosine; by ABL1 Ref.7
Natural variations
	Natural variant	415	1	S → L. Corresponds to variant rs17084492 [ dbSNP | Ensembl ].		VAR_052953
Experimental info
	Sequence conflict	307 – 308	2	PP → RR Ref.1
	Sequence conflict	307 – 308	2	PP → RR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9UPY6 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: C0655CE181BD3C57	FASTA	502	55,293
	10 20 30 40 50 60 MPLVKRNIEP RHLCRGALPE GITSELECVT NSTLAAIIRQ LSSLSKHAED IFGELFNEAN 70 80 90 100 110 120 NFYIRANSLQ DRIDRLAVKV TQLDSTVEEV SLQDINMKKA FKSSTVQDQQ VVSKNSIPNP 130 140 150 160 170 180 VADIYNQSDK PPPLNILTPY RDDKKDGLKF YTDPSYFFDL WKEKMLQDTE DKRKEKRRQK 190 200 210 220 230 240 EQKRIDGTTR EVKKVRKARN RRQEWNMMAY DKELRPDNRL SQSVYHGASS EGSLSPDTRS 250 260 270 280 290 300 HASDVTDYSY PATPNHSLHP QPVTPSYAAG DVPPHGPASQ AAEHEYRPPS ASARHMALNR 310 320 330 340 350 360 PQQPPPPPPP QAPEGSQASA PMAPADYGML PAQIIEYYNP SGPPPPPPPP VIPSAQTAFV 370 380 390 400 410 420 SPLQMPMQPP FPASASSTHA APPHPPSTGL LVTAPPPPGP PPPPPGPPGP GSSLSSSPMH 430 440 450 460 470 480 GPPVAEAKRQ EPAQPPISDA RSDLLAAIRM GIQLKKVQEQ REQEAKREPV GNDVATILSR 490 500 RIAVEYSDSD DDSEFDENDW SD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex." Suetsugu S., Miki H., Takenawa T. Biochem. Biophys. Res. Commun. 260:296-302(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Genomic organization and expression profile of the human and mouse WAVE gene family." Sossey-Alaoui K., Head K., Nowak N., Cowell J.K. Mamm. Genome 14:314-322(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Machesky L.M., Insall R.H. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-502.
[7]	"c-Abl-mediated phosphorylation of WAVE3 is required for lamellipodia formation and cell migration." Sossey-Alaoui K., Li X., Cowell J.K. J. Biol. Chem. 282:26257-26265(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT TYR-151; TYR-248; TYR-337 AND TYR-486.
[8]	"Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration." Bai S.W., Herrera-Abreu M.T., Rohn J.L., Racine V., Tajadura V., Suryavanshi N., Bechtel S., Wiemann S., Baum B., Ridley A.J. BMC Biol. 9:54-54(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026543 mRNA. Translation: BAA81796.1. AF454702 mRNA. Translation: AAL51032.1. AB020707 mRNA. Translation: BAA74923.2. Different initiation. AL163538, AL159978 Genomic DNA. Translation: CAH72487.1. AF134305 mRNA. Translation: AAD33054.1.
IPI	IPI00299003.
RefSeq	NP_006637.2. NM_006646.5.
UniGene	Hs.618732.
3D structure databases
ProteinModelPortal	Q9UPY6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UPY6. 1 interaction.
STRING	9606.ENSP00000335055.
PTM databases
PhosphoSite	Q9UPY6.
Polymorphism databases
DMDM	59800455.
Proteomic databases
PaxDb	Q9UPY6.
PRIDE	Q9UPY6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335327; ENSP00000335055; ENSG00000132970.
GeneID	10810.
KEGG	hsa:10810.
UCSC	uc001uqv.3. human.
Organism-specific databases
CTD	10810.
GeneCards	GC13P027132.
HGNC	HGNC:12734. WASF3.
HPA	CAB037086.
MIM	605068. gene.
neXtProt	NX_Q9UPY6.
PharmGKB	PA37345.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG299696.
HOGENOM	HOG000021456.
HOVERGEN	HBG058482.
InParanoid	Q9UPY6.
KO	K06083.
OMA	MEPNETS.
OrthoDB	EOG40K801.
PhylomeDB	Q9UPY6.
Gene expression databases
ArrayExpress	Q9UPY6.
Bgee	Q9UPY6.
CleanEx	HS_WASF3.
Genevestigator	Q9UPY6.
GermOnline	ENSG00000132970. Homo sapiens.
Family and domain databases
InterPro	IPR003124. WH2_dom. [Graphical view]
Pfam	PF02205. WH2. 1 hit. [Graphical view]
SMART	SM00246. WH2. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	WASF3. human.
GenomeRNAi	10810.
NextBio	41067.
PMAP-CutDB	Q9UPY6.
SOURCE	Search...

Entry information

Entry name

WASF3_HUMAN

Accession

Primary (citable) accession number: Q9UPY6
Secondary accession number(s): O94974

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.