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Q9UPY5 (XCT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cystine/glutamate transporter
Alternative name(s):
Amino acid transport system xc-
Calcium channel blocker resistance protein CCBR1
Solute carrier family 7 member 11
xCT
Gene names
Name:SLC7A11
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Ref.8

Subunit structure

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Ref.8.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Ontologies

Keywords
   Biological processAmino-acid transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-glutamate transport

Traceable author statement PubMed 10206947. Source: GOC

amino acid transport

Traceable author statement. Source: Reactome

blood coagulation

Traceable author statement. Source: Reactome

brain development

Inferred from electronic annotation. Source: Ensembl

ion transport

Traceable author statement. Source: Reactome

lens fiber cell differentiation

Inferred from electronic annotation. Source: Ensembl

leukocyte migration

Traceable author statement. Source: Reactome

platelet aggregation

Inferred from electronic annotation. Source: Ensembl

response to nicotine

Inferred from electronic annotation. Source: Ensembl

response to oxidative stress

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from direct assay PubMed 17575980. Source: UniProtKB

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentcytoskeleton

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Traceable author statement PubMed 10206947. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

rough endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncystine:glutamate antiporter activity

Traceable author statement PubMed 10206947. Source: ProtInc

protein binding

Inferred from physical interaction PubMed 21397861. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CD44P160704EBI-3843348,EBI-490245

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Cystine/glutamate transporter
PRO_0000054279

Regions

Topological domain1 – 4343Cytoplasmic Potential
Transmembrane44 – 6421Helical; Potential
Topological domain65 – 7410Extracellular Potential
Transmembrane75 – 9521Helical; Potential
Topological domain96 – 11318Cytoplasmic Potential
Transmembrane114 – 13421Helical; Potential
Topological domain135 – 15824Extracellular Potential
Transmembrane159 – 17921Helical; Potential
Topological domain180 – 18910Cytoplasmic Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 23424Extracellular Potential
Transmembrane235 – 25521Helical; Potential
Topological domain256 – 26510Cytoplasmic Potential
Transmembrane266 – 28621Helical; Potential
Topological domain287 – 31731Extracellular Potential
Transmembrane318 – 33821Helical; Potential
Topological domain339 – 36426Cytoplasmic Potential
Transmembrane365 – 38521Helical; Potential
Topological domain386 – 3872Extracellular Potential
Transmembrane388 – 40821Helical; Potential
Topological domain409 – 42214Cytoplasmic Potential
Transmembrane423 – 44321Helical; Potential
Topological domain444 – 4496Extracellular Potential
Transmembrane450 – 47021Helical; Potential
Topological domain471 – 50131Cytoplasmic Potential

Amino acid modifications

Modified residue261Phosphoserine Ref.9 Ref.10
Glycosylation3141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9UPY5 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 3EF2648B94A9F59E

FASTA50155,423
        10         20         30         40         50         60 
MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL KRKVTLLRGV SIIIGTIIGA 

        70         80         90        100        110        120 
GIFISPKGVL QNTGSVGMSL TIWTVCGVLS LFGALSYAEL GTTIKKSGGH YTYILEVFGP 

       130        140        150        160        170        180 
LPAFVRVWVE LLIIRPAATA VISLAFGRYI LEPFFIQCEI PELAIKLITA VGITVVMVLN 

       190        200        210        220        230        240 
SMSVSWSARI QIFLTFCKLT AILIIIVPGV MQLIKGQTQN FKDAFSGRDS SITRLPLAFY 

       250        260        270        280        290        300 
YGMYAYAGWF YLNFVTEEVE NPEKTIPLAI CISMAIVTIG YVLTNVAYFT TINAEELLLS 

       310        320        330        340        350        360 
NAVAVTFSER LLGNFSLAVP IFVALSCFGS MNGGVFAVSR LFYVASREGH LPEILSMIHV 

       370        380        390        400        410        420 
RKHTPLPAVI VLHPLTMIML FSGDLDSLLN FLSFARWLFI GLAVAGLIYL RYKCPDMHRP 

       430        440        450        460        470        480 
FKVPLFIPAL FSFTCLFMVA LSLYSDPFST GIGFVITLTG VPAYYLFIIW DKKPRWFRIM 

       490        500 
SEKITRTLQI ILEVVPEEDK L 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of human xCT, the light chain of amino acid transport system xc-."
Sato H., Tamba M., Kuriyama-Matsumura K., Okuno S., Bannai S.
Antioxid. Redox Signal. 2:665-671(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"CCBR1, novel CD98 light chain implicated in redox control and calcium signaling."
Conklin D.S., Beach D.H.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure, function, and regulation of human cystine/glutamate transporter in retinal pigment epithelial cells."
Bridges C.C., Kekuda R., Wang H., Prasad P.D., Mehta P., Huang W., Smith S.B., Ganapathy V.
Invest. Ophthalmol. Vis. Sci. 42:47-54(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[4]Borsani G., Manzoni M., Palacin M., Pineda M., Gasol E.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Tongue.
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[8]"Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility."
Gasol E., Jimenez-Vidal M., Chillaron J., Zorzano A., Palacin M.
J. Biol. Chem. 279:31228-31236(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MEMBRANE TOPOLOGY, SUBCELLULAR LOCATION.
[9]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB026891 mRNA. Translation: BAA82628.1.
AF200708 mRNA. Translation: AAG35592.1.
AF252872 mRNA. Translation: AAK49111.1.
AJ277882 mRNA. Translation: CAC81905.1.
AK290359 mRNA. Translation: BAF83048.1.
AK314855 mRNA. Translation: BAG37372.1.
CH471056 Genomic DNA. Translation: EAX05135.1.
BC012087 mRNA. Translation: AAH12087.1.
CCDSCCDS3742.1.
RefSeqNP_055146.1. NM_014331.3.
UniGeneHs.390594.

3D structure databases

ProteinModelPortalQ9UPY5.
SMRQ9UPY5. Positions 52-359.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117179. 2 interactions.
IntActQ9UPY5. 1 interaction.
STRING9606.ENSP00000280612.

Chemistry

BindingDBQ9UPY5.
ChEMBLCHEMBL1075149.
DrugBankDB00138. L-Cystine.
DB00142. L-Glutamic Acid.
DB00795. Sulfasalazine.

Protein family/group databases

TCDB2.A.3.8.18. the amino acid-polyamine-organocation (apc) family.

PTM databases

PhosphoSiteQ9UPY5.

Polymorphism databases

DMDM12585385.

Proteomic databases

MaxQBQ9UPY5.
PaxDbQ9UPY5.
PRIDEQ9UPY5.

Protocols and materials databases

DNASU23657.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000280612; ENSP00000280612; ENSG00000151012.
GeneID23657.
KEGGhsa:23657.
UCSCuc021xrw.1. human.

Organism-specific databases

CTD23657.
GeneCardsGC04M139085.
HGNCHGNC:11059. SLC7A11.
MIM607933. gene.
neXtProtNX_Q9UPY5.
PharmGKBPA35919.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0531.
HOGENOMHOG000098892.
HOVERGENHBG000476.
InParanoidQ9UPY5.
KOK13869.
OMAMYAYAGX.
OrthoDBEOG73BVCR.
PhylomeDBQ9UPY5.
TreeFamTF313355.

Enzyme and pathway databases

BioCycMetaCyc:ENSG00000151012-MONOMER.
ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressQ9UPY5.
BgeeQ9UPY5.
CleanExHS_SLC7A11.
GenevestigatorQ9UPY5.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PfamPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00911. 2A0308. 1 hit.
ProtoNetSearch...

Other

GeneWikiSLC7A11.
GenomeRNAi23657.
NextBio46497.
PROQ9UPY5.
SOURCESearch...

Entry information

Entry nameXCT_HUMAN
AccessionPrimary (citable) accession number: Q9UPY5
Secondary accession number(s): A8K2U4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM