Q9UPY5 (XCT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystine/glutamate transporter

Alternative name(s):
Amino acid transport system xc-
Calcium channel blocker resistance protein CCBR1
Solute carrier family 7 member 11
xCT

Gene names

Name:

SLC7A11

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Ref.8
Subunit structure	Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Ref.8.
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome brain development Inferred from electronic annotation. Source: Compara lens fiber cell differentiation Inferred from electronic annotation. Source: Compara leukocyte migration Traceable author statement. Source: Reactome platelet aggregation Inferred from electronic annotation. Source: Compara response to nicotine Inferred from electronic annotation. Source: Compara response to oxidative stress Inferred from electronic annotation. Source: Compara response to toxin Inferred from direct assay PubMed 17575980. Source: UniProtKB
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: Compara integral to membrane Traceable author statement PubMed 10206947. Source: ProtInc plasma membrane Traceable author statement. Source: Reactome rough endoplasmic reticulum Inferred from electronic annotation. Source: Compara
Molecular_function	cystine:glutamate antiporter activity Traceable author statement PubMed 10206947. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD44	P16070	4	EBI-3843348,EBI-490245

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Cystine/glutamate transporter		PRO_0000054279
Regions
	Topological domain	1 – 43	43	Cytoplasmic Potential
	Transmembrane	44 – 64	21	Helical; Potential
	Topological domain	65 – 74	10	Extracellular Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Topological domain	96 – 113	18	Cytoplasmic Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Topological domain	135 – 158	24	Extracellular Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Topological domain	180 – 189	10	Cytoplasmic Potential
	Transmembrane	190 – 210	21	Helical; Potential
	Topological domain	211 – 234	24	Extracellular Potential
	Transmembrane	235 – 255	21	Helical; Potential
	Topological domain	256 – 265	10	Cytoplasmic Potential
	Transmembrane	266 – 286	21	Helical; Potential
	Topological domain	287 – 317	31	Extracellular Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Topological domain	339 – 364	26	Cytoplasmic Potential
	Transmembrane	365 – 385	21	Helical; Potential
	Topological domain	386 – 387	2	Extracellular Potential
	Transmembrane	388 – 408	21	Helical; Potential
	Topological domain	409 – 422	14	Cytoplasmic Potential
	Transmembrane	423 – 443	21	Helical; Potential
	Topological domain	444 – 449	6	Extracellular Potential
	Transmembrane	450 – 470	21	Helical; Potential
	Topological domain	471 – 501	31	Cytoplasmic Potential
Amino acid modifications
	Modified residue	26	1	Phosphoserine Ref.9 Ref.10
	Glycosylation	314	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UPY5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3EF2648B94A9F59E	FASTA	501	55,423
	10 20 30 40 50 60 MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL KRKVTLLRGV SIIIGTIIGA 70 80 90 100 110 120 GIFISPKGVL QNTGSVGMSL TIWTVCGVLS LFGALSYAEL GTTIKKSGGH YTYILEVFGP 130 140 150 160 170 180 LPAFVRVWVE LLIIRPAATA VISLAFGRYI LEPFFIQCEI PELAIKLITA VGITVVMVLN 190 200 210 220 230 240 SMSVSWSARI QIFLTFCKLT AILIIIVPGV MQLIKGQTQN FKDAFSGRDS SITRLPLAFY 250 260 270 280 290 300 YGMYAYAGWF YLNFVTEEVE NPEKTIPLAI CISMAIVTIG YVLTNVAYFT TINAEELLLS 310 320 330 340 350 360 NAVAVTFSER LLGNFSLAVP IFVALSCFGS MNGGVFAVSR LFYVASREGH LPEILSMIHV 370 380 390 400 410 420 RKHTPLPAVI VLHPLTMIML FSGDLDSLLN FLSFARWLFI GLAVAGLIYL RYKCPDMHRP 430 440 450 460 470 480 FKVPLFIPAL FSFTCLFMVA LSLYSDPFST GIGFVITLTG VPAYYLFIIW DKKPRWFRIM 490 500 SEKITRTLQI ILEVVPEEDK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of human xCT, the light chain of amino acid transport system xc-." Sato H., Tamba M., Kuriyama-Matsumura K., Okuno S., Bannai S. Antioxid. Redox Signal. 2:665-671(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"CCBR1, novel CD98 light chain implicated in redox control and calcium signaling." Conklin D.S., Beach D.H. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure, function, and regulation of human cystine/glutamate transporter in retinal pigment epithelial cells." Bridges C.C., Kekuda R., Wang H., Prasad P.D., Mehta P., Huang W., Smith S.B., Ganapathy V. Invest. Ophthalmol. Vis. Sci. 42:47-54(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[4]	Borsani G., Manzoni M., Palacin M., Pineda M., Gasol E. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[8]	"Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility." Gasol E., Jimenez-Vidal M., Chillaron J., Zorzano A., Palacin M. J. Biol. Chem. 279:31228-31236(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MEMBRANE TOPOLOGY, SUBCELLULAR LOCATION.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026891 mRNA. Translation: BAA82628.1. AF200708 mRNA. Translation: AAG35592.1. AF252872 mRNA. Translation: AAK49111.1. AJ277882 mRNA. Translation: CAC81905.1. AK290359 mRNA. Translation: BAF83048.1. AK314855 mRNA. Translation: BAG37372.1. CH471056 Genomic DNA. Translation: EAX05135.1. BC012087 mRNA. Translation: AAH12087.1.
IPI	IPI00010474.
RefSeq	NP_055146.1. NM_014331.3.
UniGene	Hs.390594.
3D structure databases
ProteinModelPortal	Q9UPY5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UPY5. 1 interaction.
STRING	9606.ENSP00000280612.
PTM databases
PhosphoSite	Q9UPY5.
Polymorphism databases
DMDM	12585385.
Proteomic databases
PaxDb	Q9UPY5.
PRIDE	Q9UPY5.
Protocols and materials databases
DNASU	23657.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000280612; ENSP00000280612; ENSG00000151012.
GeneID	23657.
KEGG	hsa:23657.
UCSC	uc021xrw.1. human.
Organism-specific databases
CTD	23657.
GeneCards	GC04M139085.
HGNC	HGNC:11059. SLC7A11.
MIM	607933. gene.
neXtProt	NX_Q9UPY5.
PharmGKB	PA35919.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0531.
HOGENOM	HOG000098892.
HOVERGEN	HBG000476.
InParanoid	Q9UPY5.
KO	K13869.
OMA	SLLNFMS.
OrthoDB	EOG4F1X35.
PhylomeDB	Q9UPY5.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000151012-MONOMER.
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q9UPY5.
Bgee	Q9UPY5.
CleanEx	HS_SLC7A11.
Genevestigator	Q9UPY5.
GermOnline	ENSG00000151012. Homo sapiens.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004760. L_AA_transporter. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00911. 2A0308. 1 hit.
ProtoNet	Search...
Other
BindingDB	Q9UPY5.
ChEMBL	CHEMBL1075149.
DrugBank	DB00138. L-Cystine. DB00142. L-Glutamic Acid. DB00795. Sulfasalazine.
GenomeRNAi	23657.
NextBio	46497.
SOURCE	Search...

Entry information

Entry name

XCT_HUMAN

Accession

Primary (citable) accession number: Q9UPY5
Secondary accession number(s): A8K2U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.