Q9UPY3 (DICER_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoribonuclease Dicer
EC=3.1.26.-

Alternative name(s):
Helicase with RNase motif
Short name=Helicase MOI

Gene names

Name:	DICER1
Synonyms:	DICER, HERNA, KIAA0928

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1922 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.23 Ref.24
Cofactor	Binds 2 magnesium or manganese ions per subunit Probable. Ref.30
Subunit structure	Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, EIF2C1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D. Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.23 Ref.28 Ref.30
Subcellular location	Cytoplasm Ref.17.
Involvement in disease	Pleuropulmonary blastoma (PPB) [MIM:601200]: A rare pediatric intrathoracic neoplasm. The tumor arises from the lung, pleura, or both, and appears to be purely mesenchymal in phenotype. It lacks malignant epithelial elements, a feature that distinguishes it from the classic adult-type pulmonary blastoma. It arises during fetal lung development and is often part of an inherited cancer syndrome. The tumor contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis. Some patients have multilocular cystic nephroma, a benign kidney tumor. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.26 Ref.31 Goiter multinodular 1, with or without Sertoli-Leydig cell tumors (MNG1) [MIM:138800]: A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.26 Ref.32 DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (Ref.26). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (Ref.29). Ref.26
Sequence similarities	Belongs to the helicase family. Dicer subfamily. Contains 1 Dicer dsRNA-binding fold domain. Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 PAZ domain. Contains 2 RNase III domains.
Caution	It is uncertain whether Met-1 or Met-11 is the initiator.
Sequence caution	The sequence CAB38857.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Disease	Disease mutation
Domain	Repeat
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: Compara apoptotic process Inferred from electronic annotation. Source: Compara branching morphogenesis of an epithelial tube Inferred from electronic annotation. Source: Compara cardiac muscle cell development Inferred from electronic annotation. Source: Compara cerebral cortex development Inferred from electronic annotation. Source: Compara defense response to virus Inferred from electronic annotation. Source: Compara embryonic hindlimb morphogenesis Inferred from electronic annotation. Source: Compara hair follicle cell proliferation Inferred from electronic annotation. Source: Compara hair follicle morphogenesis Inferred from electronic annotation. Source: Compara inner ear receptor cell development Inferred from electronic annotation. Source: Compara intestinal epithelial cell development Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara multicellular organism growth Inferred from electronic annotation. Source: Compara negative regulation of Schwann cell proliferation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: BHF-UCL nerve development Inferred from sequence or structural similarity. Source: BHF-UCL neuron projection morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL olfactory bulb interneuron differentiation Inferred from electronic annotation. Source: Compara peripheral nervous system myelin formation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of Schwann cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of miRNA metabolic process Inferred from electronic annotation. Source: Compara positive regulation of myelination Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara post-embryonic development Inferred from electronic annotation. Source: Compara pre-miRNA processing Inferred from direct assay Ref.16. Source: UniProtKB production of siRNA involved in RNA interference Inferred from direct assay Ref.16Ref.18. Source: UniProtKB regulation of cell cycle Inferred from electronic annotation. Source: Compara regulation of enamel mineralization Inferred from electronic annotation. Source: Compara regulation of neuron differentiation Inferred from electronic annotation. Source: Compara regulation of oligodendrocyte differentiation Inferred from electronic annotation. Source: Compara regulation of viral genome replication Inferred from electronic annotation. Source: Compara spinal cord motor neuron differentiation Inferred from electronic annotation. Source: Compara spindle assembly Inferred from electronic annotation. Source: Compara spleen development Inferred from electronic annotation. Source: Compara stem cell maintenance Inferred from electronic annotation. Source: Compara targeting of mRNA for destruction involved in RNA interference Inferred from mutant phenotype Ref.16Ref.18. Source: UniProtKB zygote asymmetric cell division Inferred from electronic annotation. Source: Compara
Cellular_component	RNA-induced silencing complex Inferred from direct assay Ref.16. Source: UniProtKB cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro double-stranded RNA binding Inferred from direct assay PubMed 12411504. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW miRNA binding Inferred from electronic annotation. Source: Compara ribonuclease III activity Inferred from direct assay PubMed 12560494. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DHX58	Q96C10	2	EBI-395506,EBI-744193
EIF2AK2	P19525	2	EBI-395506,EBI-640775
EIF2C1	Q9UL18	3	EBI-395506,EBI-527363
EIF2C2	Q9UKV8	7	EBI-395506,EBI-528269
Eif2c2	Q8CJG0	2	EBI-395506,EBI-528299	From a different organism.
PIWIL1	Q96J94	2	EBI-395506,EBI-527417
TARBP2	Q15633	8	EBI-395506,EBI-978581

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UPY3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UPY3-2)

Also known as: t-Dicer;

The sequence of this isoform differs from the canonical sequence as follows:
     1789-1922: LRRSEEDEEK...KANQPQVPNS → KSFLQMYPVP...TTGRSESLWK

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1922

1922

Endoribonuclease Dicer

PRO_0000180470

Regions

Domain

51 – 227

177

Helicase ATP-binding

Domain

433 – 602

170

Helicase C-terminal

Domain

630 – 722

93

Dicer dsRNA-binding fold

Domain

891 – 1042

152

PAZ

Domain

1276 – 1403

128

RNase III 1

Domain

1666 – 1824

159

RNase III 2

Domain

1849 – 1914

66

DRBM

Nucleotide binding

64 – 71

8

ATP By similarity

Region

256 – 595

340

Required for interaction with PRKRA and TARBP2

Motif

175 – 178

4

DECH box

Sites

Metal binding

1316

1

Magnesium or manganese 1 By similarity

Metal binding

1395

1

Magnesium or manganese 1 By similarity

Metal binding

1398

1

Magnesium or manganese 1 By similarity

Metal binding

1705

1

Magnesium or manganese 2

Metal binding

1810

1

Magnesium or manganese 2

Metal binding

1813

1

Magnesium or manganese 2

Site

1806

1

Important for activity By similarity

Amino acid modifications

Modified residue

413

1

Phosphoserine Ref.27

Modified residue

415

1

Phosphoserine Ref.27

Modified residue

1016

1

Phosphoserine Ref.22

Natural variations

Alternative sequence

1789 – 1922

134

LRRSE…QVPNS → KSFLQMYPVPLCENCLKWNQ KLPNLARLRELTTGRSESLW K in isoform 2.

VSP_042832

Natural variant

839

1

S → F in MNG1. Ref.32

VAR_065301

Natural variant

1583

1

L → R in PPB. Ref.31

VAR_063150

Natural variant

1705

1

E → K in non-epithelial ovarian tumor; somatic mutation; results in reduced RNase IIIb activity but retention of RNase IIIa activity. Ref.29

VAR_067091

Natural variant

1709

1

D → E in non-epithelial ovarian tumor; somatic mutation; results in reduced RNase IIIb activity but retention of RNase IIIa activity. Ref.29

VAR_067092

Natural variant

1709

1

D → G in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067093

Natural variant

1709

1

D → N in non-epithelial ovarian tumor; somatic mutation; results in reduced RNase IIIb activity but retention of RNase IIIa activity. Ref.29

VAR_067094

Natural variant

1810

1

D → H in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067095

Natural variant

1810

1

D → N in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067096

Natural variant

1810

1

D → Y in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067097

Natural variant

1813

1

E → G in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067098

Natural variant

1813

1

E → K in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067099

Natural variant

1813

1

E → Q in non-epithelial ovarian tumor; somatic mutation. Ref.29

VAR_067100

Experimental info

Mutagenesis

960

1

F → A: 2-fold decrease in activity.

Mutagenesis

971

1

Y → A: 10-fold decrease in activity; when associated with Y-972.

Mutagenesis

972

1

Y → A: 10-fold decrease in activity; when associated with Y-971.

Mutagenesis

1036

1

E → A: 5-fold decrease in activity.

Mutagenesis

1313

1

E → A: No effect on activity. Ref.10

Mutagenesis

1320

1

D → A: Decreased activity. Loss of activity; when associated with D-1709. Ref.10

Mutagenesis

1340

1

E → A: No effect on activity. Ref.10

Mutagenesis

1444

1

E → A: Decreased activity. Loss of activity; when associated with E-1813. Ref.10

Mutagenesis

1702

1

Q → A: No effect on activity. Ref.10

Mutagenesis

1709

1

D → A: Decreased activity. Loss of activity; when associated with D-1320. Ref.10

Mutagenesis

1729

1

P → E: No effect on activity. Ref.10

Mutagenesis

1813

1

E → A: Decreased activity. Loss of activity; when associated with E-1444. Ref.10

Sequence conflict

75 – 90

16

VLLTK…RGDFS → STTLLKSCLYLDLGETSA in BAA78691. Ref.1

Sequence conflict

189

1

I → F in BAA78691. Ref.1

Sequence conflict

195

1

N → I in BAA78691. Ref.1

Sequence conflict

214

1

C → W in BAA78691. Ref.1

Sequence conflict

218

1

E → D in BAA78691. Ref.1

Sequence conflict

223

1

I → F in BAA78691. Ref.1

Sequence conflict

393 – 394

2

QQ → HS in BAA78691. Ref.1

Sequence conflict

492 – 493

2

KQ → NT in BAA78691. Ref.1

Sequence conflict

609

1

D → H in BAA78691. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1922

Helix Strand Turn

Details...

Turn

1662 – 1665

4

Helix

1666 – 1673

8

Helix

1680 – 1687

8

Helix

1702 – 1722

21

Helix

1729 – 1739

11

Helix

1742 – 1751

10

Helix

1754 – 1756

3

Helix

1763 – 1778

16

Helix

1806 – 1822

17

Helix

1827 – 1842

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 3. Checksum: 9452B96A601D4551	FASTA	1,922	218,682
	10 20 30 40 50 60 MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT 70 80 90 100 110 120 IVCLNTGSGK TFIAVLLTKE LSYQIRGDFS RNGKRTVFLV NSANQVAQQV SAVRTHSDLK 130 140 150 160 170 180 VGEYSNLEVN ASWTKERWNQ EFTKHQVLIM TCYVALNVLK NGYLSLSDIN LLVFDECHLA 190 200 210 220 230 240 ILDHPYREIM KLCENCPSCP RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL 250 260 270 280 290 300 VVLDRYTSQP CEIVVDCGPF TDRSGLYERL LMELEEALNF INDCNISVHS KERDSTLISK 310 320 330 340 350 360 QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT FLRKIHALCE 370 380 390 400 410 420 EHFSPASLDL KFVTPKVIKL LEILRKYKPY ERQQFESVEW YNNRNQDNYV SWSDSEDDDE 430 440 450 460 470 480 DEEIEEKEKP ETNFPSPFTN ILCGIIFVER RYTAVVLNRL IKEAGKQDPE LAYISSNFIT 490 500 510 520 530 540 GHGIGKNQPR NKQMEAEFRK QEEVLRKFRA HETNLLIATS IVEEGVDIPK CNLVVRFDLP 550 560 570 580 590 600 TEYRSYVQSK GRARAPISNY IMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSVDTGETD 610 620 630 640 650 660 IDPVMDDDDV FPPYVLRPDD GGPRVTINTA IGHINRYCAR LPSDPFTHLA PKCRTRELPD 670 680 690 700 710 720 GTFYSTLYLP INSPLRASIV GPPMSCVRLA ERVVALICCE KLHKIGELDD HLMPVGKETV 730 740 750 760 770 780 KYEEELDLHD EEETSVPGRP GSTKRRQCYP KAIPECLRDS YPRPDQPCYL YVIGMVLTTP 790 800 810 820 830 840 LPDELNFRRR KLYPPEDTTR CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFMLSL 850 860 870 880 890 900 QMLELITRLH QYIFSHILRL EKPALEFKPT DADSAYCVLP LNVVNDSSTL DIDFKFMEDI 910 920 930 940 950 960 EKSEARIGIP STKYTKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV YTDLTPLSKF 970 980 990 1000 1010 1020 PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL TPRHLNQKGK ALPLSSAEKR 1030 1040 1050 1060 1070 1080 KAKWESLQNK QILVPELCAI HPIPASLWRK AVCLPSILYR LHCLLTAEEL RAQTASDAGV 1090 1100 1110 1120 1130 1140 GVRSLPADFR YPNLDFGWKK SIDSKSFISI SNSSSAENDN YCKHSTIVPE NAAHQGANRT 1150 1160 1170 1180 1190 1200 SSLENHDQMS VNCRTLLSES PGKLHVEVSA DLTAINGLSY NQNLANGSYD LANRDFCQGN 1210 1220 1230 1240 1250 1260 QLNYYKQEIP VQPTTSYSIQ NLYSYENQPQ PSDECTLLSN KYLDGNANKS TSDGSPVMAV 1270 1280 1290 1300 1310 1320 MPGTTDTIQV LKGRMDSEQS PSIGYSSRTL GPNPGLILQA LTLSNASDGF NLERLEMLGD 1330 1340 1350 1360 1370 1380 SFLKHAITTY LFCTYPDAHE GRLSYMRSKK VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW 1390 1400 1410 1420 1430 1440 LPPGYVVNQD KSNTDKWEKD EMTKDCMLAN GKLDEDYEEE DEEEESLMWR APKEEADYED 1450 1460 1470 1480 1490 1500 DFLEYDQEHI RFIDNMLMGS GAFVKKISLS PFSTTDSAYE WKMPKKSSLG SMPFSSDFED 1510 1520 1530 1540 1550 1560 FDYSSWDAMC YLDPSKAVEE DDFVVGFWNP SEENCGVDTG KQSISYDLHT EQCIADKSIA 1570 1580 1590 1600 1610 1620 DCVEALLGCY LTSCGERAAQ LFLCSLGLKV LPVIKRTDRE KALCPTRENF NSQQKNLSVS 1630 1640 1650 1660 1670 1680 CAAASVASSR SSVLKDSEYG CLKIPPRCMF DHPDADKTLN HLISGFENFE KKINYRFKNK 1690 1700 1710 1720 1730 1740 AYLLQAFTHA SYHYNTITDC YQRLEFLGDA ILDYLITKHL YEDPRQHSPG VLTDLRSALV 1750 1760 1770 1780 1790 1800 NNTIFASLAV KYDYHKYFKA VSPELFHVID DFVQFQLEKN EMQGMDSELR RSEEDEEKEE 1810 1820 1830 1840 1850 1860 DIEVPKAMGD IFESLAGAIY MDSGMSLETV WQVYYPMMRP LIEKFSANVP RSPVRELLEM 1870 1880 1890 1900 1910 1920 EPETAKFSPA ERTYDGKVRV TVEVVGKGKF KGVGRSYRIA KSAAARRALR SLKANQPQVP NS
	Isoform 2 (t-Dicer) [UniParc]. Checksum: 8F52FAB99C876358	FASTA	1,829	208,448
	10 20 30 40 50 60 MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT 70 80 90 100 110 120 IVCLNTGSGK TFIAVLLTKE LSYQIRGDFS RNGKRTVFLV NSANQVAQQV SAVRTHSDLK 130 140 150 160 170 180 VGEYSNLEVN ASWTKERWNQ EFTKHQVLIM TCYVALNVLK NGYLSLSDIN LLVFDECHLA 190 200 210 220 230 240 ILDHPYREIM KLCENCPSCP RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL 250 260 270 280 290 300 VVLDRYTSQP CEIVVDCGPF TDRSGLYERL LMELEEALNF INDCNISVHS KERDSTLISK 310 320 330 340 350 360 QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT FLRKIHALCE 370 380 390 400 410 420 EHFSPASLDL KFVTPKVIKL LEILRKYKPY ERQQFESVEW YNNRNQDNYV SWSDSEDDDE 430 440 450 460 470 480 DEEIEEKEKP ETNFPSPFTN ILCGIIFVER RYTAVVLNRL IKEAGKQDPE LAYISSNFIT 490 500 510 520 530 540 GHGIGKNQPR NKQMEAEFRK QEEVLRKFRA HETNLLIATS IVEEGVDIPK CNLVVRFDLP 550 560 570 580 590 600 TEYRSYVQSK GRARAPISNY IMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSVDTGETD 610 620 630 640 650 660 IDPVMDDDDV FPPYVLRPDD GGPRVTINTA IGHINRYCAR LPSDPFTHLA PKCRTRELPD 670 680 690 700 710 720 GTFYSTLYLP INSPLRASIV GPPMSCVRLA ERVVALICCE KLHKIGELDD HLMPVGKETV 730 740 750 760 770 780 KYEEELDLHD EEETSVPGRP GSTKRRQCYP KAIPECLRDS YPRPDQPCYL YVIGMVLTTP 790 800 810 820 830 840 LPDELNFRRR KLYPPEDTTR CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFMLSL 850 860 870 880 890 900 QMLELITRLH QYIFSHILRL EKPALEFKPT DADSAYCVLP LNVVNDSSTL DIDFKFMEDI 910 920 930 940 950 960 EKSEARIGIP STKYTKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV YTDLTPLSKF 970 980 990 1000 1010 1020 PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL TPRHLNQKGK ALPLSSAEKR 1030 1040 1050 1060 1070 1080 KAKWESLQNK QILVPELCAI HPIPASLWRK AVCLPSILYR LHCLLTAEEL RAQTASDAGV 1090 1100 1110 1120 1130 1140 GVRSLPADFR YPNLDFGWKK SIDSKSFISI SNSSSAENDN YCKHSTIVPE NAAHQGANRT 1150 1160 1170 1180 1190 1200 SSLENHDQMS VNCRTLLSES PGKLHVEVSA DLTAINGLSY NQNLANGSYD LANRDFCQGN 1210 1220 1230 1240 1250 1260 QLNYYKQEIP VQPTTSYSIQ NLYSYENQPQ PSDECTLLSN KYLDGNANKS TSDGSPVMAV 1270 1280 1290 1300 1310 1320 MPGTTDTIQV LKGRMDSEQS PSIGYSSRTL GPNPGLILQA LTLSNASDGF NLERLEMLGD 1330 1340 1350 1360 1370 1380 SFLKHAITTY LFCTYPDAHE GRLSYMRSKK VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW 1390 1400 1410 1420 1430 1440 LPPGYVVNQD KSNTDKWEKD EMTKDCMLAN GKLDEDYEEE DEEEESLMWR APKEEADYED 1450 1460 1470 1480 1490 1500 DFLEYDQEHI RFIDNMLMGS GAFVKKISLS PFSTTDSAYE WKMPKKSSLG SMPFSSDFED 1510 1520 1530 1540 1550 1560 FDYSSWDAMC YLDPSKAVEE DDFVVGFWNP SEENCGVDTG KQSISYDLHT EQCIADKSIA 1570 1580 1590 1600 1610 1620 DCVEALLGCY LTSCGERAAQ LFLCSLGLKV LPVIKRTDRE KALCPTRENF NSQQKNLSVS 1630 1640 1650 1660 1670 1680 CAAASVASSR SSVLKDSEYG CLKIPPRCMF DHPDADKTLN HLISGFENFE KKINYRFKNK 1690 1700 1710 1720 1730 1740 AYLLQAFTHA SYHYNTITDC YQRLEFLGDA ILDYLITKHL YEDPRQHSPG VLTDLRSALV 1750 1760 1770 1780 1790 1800 NNTIFASLAV KYDYHKYFKA VSPELFHVID DFVQFQLEKN EMQGMDSEKS FLQMYPVPLC 1810 1820 ENCLKWNQKL PNLARLRELT TGRSESLWK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase." Matsuda S., Ichigotani Y., Okuda T., Irimura T., Nakatsugawa S., Hamaguchi M. Biochim. Biophys. Acta 1490:163-169(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A novel splice variant of the human dicer gene is expressed in neuroblastoma cells." Potenza N., Papa U., Scaruffi P., Mosca N., Tonini G.P., Russo A. FEBS Lett. 584:3452-3457(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE SPLICING. Tissue: Neuroblastoma.
[3]	"RNA binding and processing by recombinant human Dicer." Provost P., Dishart D., Doucet D., Hermansson A., Frendewey D., Samuelsson B., Radmark O. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lung.
[4]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[6]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]	"Interaction of 5-lipoxygenase with cellular proteins." Provost P., Samuelsson B., Radmark O. Proc. Natl. Acad. Sci. U.S.A. 96:1881-1885(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1248-1922 (ISOFORM 1). Tissue: Lung.
[10]	"Single processing center models for human Dicer and bacterial RNase III." Zhang H., Kolb F.A., Jaskiewicz L., Westhof E., Filipowicz W. Cell 118:57-68(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLU-1313; ASP-1320; GLU-1340; GLU-1444; GLN-1702; ASP-1709; PRO-1729 AND GLU-1813.
[11]	"Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer." Tahbaz N., Kolb F.A., Zhang H., Jaronczyk K., Filipowicz W., Hobman T.C. EMBO Rep. 5:189-194(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PIWIL1.
[12]	"Human RISC couples microRNA biogenesis and posttranscriptional gene silencing." Gregory R.I., Chendrimada T.P., Cooch N., Shiekhattar R. Cell 123:631-640(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH EIF2C2 AND TARBP2.
[13]	"Identification of novel argonaute-associated proteins." Meister G., Landthaler M., Peters L., Chen P.Y., Urlaub H., Luehrmann R., Tuschl T. Curr. Biol. 15:2149-2155(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH EIF2C1 AND EIF2C2.
[14]	"TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing." Haase A.D., Jaskiewicz L., Zhang H., Laine S., Sack R., Gatignol A., Filipowicz W. EMBO Rep. 6:961-967(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TARBP2.
[15]	"A human, ATP-independent, RISC assembly machine fueled by pre-miRNA." Maniataki E., Mourelatos Z. Genes Dev. 19:2979-2990(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH EIF2C2.
[16]	"TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing." Chendrimada T.P., Gregory R.I., Kumaraswamy E., Norman J., Cooch N., Nishikura K., Shiekhattar R. Nature 436:740-744(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH EIF2C2 AND TARBP2.
[17]	"The role of PACT in the RNA silencing pathway." Lee Y., Hur I., Park S.-Y., Kim Y.-K., Suh M.R., Kim V.N. EMBO J. 25:522-532(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PRKRA AND TARBP2, SUBCELLULAR LOCATION.
[18]	"Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA." Kok K.H., Ng M.-H., Ching Y.-P., Jin D.-Y. J. Biol. Chem. 282:17649-17657(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PRKRA AND TARBP2.
[19]	"RNA helicase A interacts with RISC in human cells and functions in RISC loading." Robb G.B., Rana T.M. Mol. Cell 26:523-537(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DHX9.
[20]	"MicroRNA silencing through RISC recruitment of eIF6." Chendrimada T.P., Finn K.J., Ji X., Baillat D., Gregory R.I., Liebhaber S.A., Pasquinelli A.E., Shiekhattar R. Nature 447:823-828(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH EIF2C2; EIF6; MOV10 AND TARBP2, ASSOCIATION WITH THE 60S RIBOSOME.
[21]	"Prolyl 4-hydroxylation regulates Argonaute 2 stability." Qi H.H., Ongusaha P.P., Myllyharju J., Cheng D., Pakkanen O., Shi Y., Lee S.W., Peng J., Shi Y. Nature 455:421-424(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH EIF2C2.
[22]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1016, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[23]	"In vitro reconstitution of the human RISC-loading complex." MacRae I.J., Ma E., Zhou M., Robinson C.V., Doudna J.A. Proc. Natl. Acad. Sci. U.S.A. 105:512-517(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH EIF2C2 AND TARBP2.
[24]	"A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function." Melo S.A., Ropero S., Moutinho C., Aaltonen L.A., Yamamoto H., Calin G.A., Rossi S., Fernandez A.F., Carneiro F., Oliveira C., Ferreira B., Liu C.-G., Villanueva A., Capella G., Schwartz S. Jr., Shiekhattar R., Esteller M. Nat. Genet. 41:365-370(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[25]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[26]	"Extending the phenotypes associated with DICER1 mutations." Foulkes W.D., Bahubeshi A., Hamel N., Pasini B., Asioli S., Baynam G., Choong C.S., Charles A., Frieder R.P., Dishop M.K., Graf N., Ekim M., Bouron-Dal Soglio D., Arseneau J., Young R.H., Sabbaghian N., Srivastava A., Tischkowitz M.D., Priest J.R. Hum. Mutat. 32:1381-1384(2011) [PubMed] [Europe PMC] [Abstract] Cited for: DISEASE.
[27]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413 AND SER-415, MASS SPECTROMETRY.
[28]	"Human RNA methyltransferase BCDIN3D regulates microRNA processing." Xhemalce B., Robson S.C., Kouzarides T. Cell 151:278-288(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BCDIN3D.
[29]	"Recurrent somatic DICER1 mutations in nonepithelial ovarian cancers." Heravi-Moussavi A., Anglesio M.S., Cheng S.W., Senz J., Yang W., Prentice L., Fejes A.P., Chow C., Tone A., Kalloger S.E., Hamel N., Roth A., Ha G., Wan A.N., Maines-Bandiera S., Salamanca C., Pasini B., Clarke B.A. , Lee A.F., Lee C.H., Zhao C., Young R.H., Aparicio S.A., Sorensen P.H., Woo M.M., Boyd N., Jones S.J., Hirst M., Marra M.A., Gilks B., Shah S.P., Foulkes W.D., Morin G.B., Huntsman D.G. N. Engl. J. Med. 366:234-242(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN NON-EPITHELIAL OVARIAN TUMORS, VARIANTS LYS-1705; ASN-1709; GLU-1709; GLY-1709; HIS-1810; TYR-1810; ASN-1810; GLN-1813; GLY-1813 AND LYS-1813, CHARACTERIZATION OF VARIANTS LYS-1705; ASN-1709 AND GLU-1709.
[30]	"Homodimeric structure and double-stranded RNA cleavage activity of the C-terminal RNase III domain of human dicer." Takeshita D., Zenno S., Lee W.C., Nagata K., Saigo K., Tanokura M. J. Mol. Biol. 374:106-120(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1660-1852, COFACTOR, SUBUNIT.
[31]	"DICER1 mutations in familial pleuropulmonary blastoma." Hill D.A., Ivanovich J., Priest J.R., Gurnett C.A., Dehner L.P., Desruisseau D., Jarzembowski J.A., Wikenheiser-Brokamp K.A., Suarez B.K., Whelan A.J., Williams G., Bracamontes D., Messinger Y., Goodfellow P.J. Science 325:965-965(2009) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT PPB ARG-1583.
[32]	"DICER1 mutations in familial multinodular goiter with and without ovarian Sertoli-Leydig cell tumors." Rio Frio T., Bahubeshi A., Kanellopoulou C., Hamel N., Niedziela M., Sabbaghian N., Pouchet C., Gilbert L., O'Brien P.K., Serfas K., Broderick P., Houlston R.S., Lesueur F., Bonora E., Muljo S., Schimke R.N., Bouron-Dal Soglio D., Arseneau J. , Schultz K.A., Priest J.R., Nguyen V.H., Harach H.R., Livingston D.M., Foulkes W.D., Tischkowitz M. JAMA 305:68-77(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT MNG1 PHE-839.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The dark side of RNA - Issue 87 of October 2007

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB028449 mRNA. Translation: BAA78691.1.
HM595745 mRNA. Translation: ADK25182.1.
AJ132261 mRNA. Translation: CAB38857.2. Different initiation.
AB023145 mRNA. Translation: BAA76772.2.
AL356017 Genomic DNA. No translation available.
AL390254 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81596.1.
BC150287 mRNA. Translation: AAI50288.1.

IPI

IPI00219036.
IPI00977554.

RefSeq

NP_001182502.1. NM_001195573.1.
NP_001258211.1. NM_001271282.1.
NP_085124.2. NM_030621.3.
NP_803187.1. NM_177438.2.

UniGene

Hs.87889.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EB1	X-ray	2.00	A/B/C	1660-1842	[»]

ProteinModelPortal

Q9UPY3.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29664N.

IntAct

Q9UPY3. 13 interactions.

MINT

MINT-1957525.

STRING

9606.ENSP00000343745.

PTM databases

PhosphoSite

Q9UPY3.

Polymorphism databases

DMDM

257051056.

Proteomic databases

PaxDb

Q9UPY3.

PRIDE

Q9UPY3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000343455; ENSP00000343745; ENSG00000100697.
ENST00000393063; ENSP00000376783; ENSG00000100697.
ENST00000526495; ENSP00000437256; ENSG00000100697.
ENST00000527414; ENSP00000435681; ENSG00000100697.
ENST00000541352; ENSP00000444719; ENSG00000100697.

GeneID

23405.

KEGG

hsa:23405.

UCSC

uc001ydv.2. human.

Organism-specific databases

CTD

23405.

GeneCards

GC14M095552.

HGNC

HGNC:17098. DICER1.

HPA

HPA000694.

MIM

138800. phenotype.
601200. phenotype.
606241. gene.

neXtProt

NX_Q9UPY3.

Orphanet

276399. Familial multinodular goiter.
99916. Ovarian malignant Sertoli-Leydig cell tumor.
284343. Pleuropulmonary blastoma family tumor susceptibility syndrome.

PharmGKB

PA38437.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1111.

HOGENOM

HOG000001567.

HOVERGEN

HBG107811.

InParanoid

Q9UPY3.

KO

K11592.

OMA

FLEYDQE.

OrthoDB

EOG4JDH5W.

PhylomeDB

Q9UPY3.

Enzyme and pathway databases

BRENDA

3.1.26.3. 2681.

Reactome

REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q9UPY3.

Bgee

Q9UPY3.

CleanEx

HS_DICER1.

Genevestigator

Q9UPY3.

GermOnline

ENSG00000100697. Homo sapiens.

Family and domain databases

Gene3D

1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.

InterPro

IPR005034. Dicer_dsRNA_binding_fold.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR003100. PAZ.
IPR000999. RNase_III_dom.
[Graphical view]

Pfam

PF00270. DEAD. 1 hit.
PF03368. dsRNA_bind. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]

SUPFAM

SSF101690. PAZ. 1 hit.
SSF69065. RNase_III. 2 hits.

PROSITE

PS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

DICER1. human.

EvolutionaryTrace

Q9UPY3.

GenomeRNAi

23405.

NextBio

45577.

SOURCE

Search...

Entry information

Entry name

DICER_HUMAN

Accession

Primary (citable) accession number: Q9UPY3
Secondary accession number(s): A7E2D3 , E0AD28, O95943, Q9UQ02

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.