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Protein

Trafficking kinesin-binding protein 1

Gene

TRAK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors.1 Publication

GO - Biological processi

  1. endosome to lysosome transport Source: UniProtKB
  2. protein O-linked glycosylation Source: UniProtKB
  3. protein targeting Source: UniProtKB
  4. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking kinesin-binding protein 1
Alternative name(s):
106 kDa O-GlcNAc transferase-interacting protein
Gene namesi
Name:TRAK1
Synonyms:KIAA1042, OIP106
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:29947. TRAK1.

Subcellular locationi

Cytoplasm. Nucleus. Mitochondrion. Early endosome. Endosome
Note: Predominantly associated with early endosome. The localization to early endosomes depends on its interaction with HRS. Colocalizes with MGARP at the mitochondria.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. early endosome Source: UniProtKB
  3. mitochondrion Source: UniProtKB
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394593.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Trafficking kinesin-binding protein 1PRO_0000058037Add
BLAST

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9UPV9.
PaxDbiQ9UPV9.
PRIDEiQ9UPV9.

PTM databases

PhosphoSiteiQ9UPV9.

Expressioni

Tissue specificityi

High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined.1 Publication

Gene expression databases

BgeeiQ9UPV9.
CleanExiHS_TRAK1.
ExpressionAtlasiQ9UPV9. baseline and differential.
GenevestigatoriQ9UPV9.

Organism-specific databases

HPAiHPA005853.

Interactioni

Subunit structurei

Interacts with O-GlcNAc transferase. Interacts with RHOT1/Miro-1 and RHOT2/Miro-2. Interacts with HGS. Interacts with GABRA1 (By similarity). Interacts with KIF5C.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHOT1Q8IXI23EBI-1105048,EBI-1396430

Protein-protein interaction databases

BioGridi116570. 7 interactions.
IntActiQ9UPV9. 8 interactions.
MINTiMINT-1195439.
STRINGi9606.ENSP00000328998.

Structurei

3D structure databases

ProteinModelPortaliQ9UPV9.
SMRiQ9UPV9. Positions 88-113, 315-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 354308HAP1 N-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni359 – 509151Interaction with HGSAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili104 – 356253Sequence AnalysisAdd
BLAST
Coiled coili492 – 53241Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi200 – 2034Poly-Ser

Domaini

The C-terminal region is required for the early endosomal and mitochondrial localization.

Sequence similaritiesi

Contains 1 HAP1 N-terminal domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG246318.
GeneTreeiENSGT00390000003132.
HOGENOMiHOG000143414.
HOVERGENiHBG069248.
InParanoidiQ9UPV9.
KOiK15369.
OMAiACGSTSH.
OrthoDBiEOG7CRTSM.
PhylomeDBiQ9UPV9.
TreeFamiTF323495.

Family and domain databases

InterProiIPR006933. HAP1_N.
IPR022154. Traffickng_kinesin-bd_prot_dom.
[Graphical view]
PfamiPF04849. HAP1_N. 1 hit.
PF12448. Milton. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UPV9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVFQFGQP VRAQPLPGLC HGKLIRTNAC DVCNSTDLPE VEIISLLEEQ
60 70 80 90 100
LPHYKLRADT IYGYDHDDWL HTPLISPDAN IDLTTEQIEE TLKYFLLCAE
110 120 130 140 150
RVGQMTKTYN DIDAVTRLLE EKERDLELAA RIGQSLLKKN KTLTERNELL
160 170 180 190 200
EEQVEHIREE VSQLRHELSM KDELLQFYTS AAEESEPESV CSTPLKRNES
210 220 230 240 250
SSSVQNYFHL DSLQKKLKDL EEENVVLRSE ASQLKTETIT YEEKEQQLVN
260 270 280 290 300
DCVKELRDAN VQIASISEEL AKKTEDAARQ QEEITHLLSQ IVDLQKKAKA
310 320 330 340 350
CAVENEELVQ HLGAAKDAQR QLTAELRELE DKYAECMEML HEAQEELKNL
360 370 380 390 400
RNKTMPNTTS RRYHSLGLFP MDSLAAEIEG TMRKELQLEE AESPDITHQK
410 420 430 440 450
RVFETVRNIN QVVKQRSLTP SPMNIPGSNQ SSAMNSLLSS CVSTPRSSFY
460 470 480 490 500
GSDIGNVVLD NKTNSIILET EAADLGNDER SKKPGTPGTP GSHDLETALR
510 520 530 540 550
RLSLRRENYL SERRFFEEEQ ERKLQELAEK GELRSGSLTP TESIMSLGTH
560 570 580 590 600
SRFSEFTGFS GMSFSSRSYL PEKLQIVKPL EGSATLHHWQ QLAQPHLGGI
610 620 630 640 650
LDPRPGVVTK GFRTLDVDLD EVYCLNDFEE DDTGDHISLP RLATSTPVQH
660 670 680 690 700
PETSAHHPGK CMSQTNSTFT FTTCRILHPS DELTRVTPSL NSAPTPACGS
710 720 730 740 750
TSHLKSTPVA TPCTPRRLSL AESFTNTRES TTTMSTSLGL VWLLKERGIS
760 770 780 790 800
AAVYDPQSWD RAGRGSLLHS YTPKMAVIPS TPPNSPMQTP TSSPPSFEFK
810 820 830 840 850
CTSPPYDNFL ASKPASSILR EVREKNVRSS ESQTDVSVSN LNLVDKVRRF
860 870 880 890 900
GVAKVVNSGR AHVPTLTEEQ GPLLCGPPGP APALVPRGLV PEGLPLRCPT
910 920 930 940 950
VTSAIGGLQL NSGIRRNRSF PTMVGSSMQM KAPVTLTSGI LMGAKLSKQT

SLR
Length:953
Mass (Da):106,040
Last modified:May 1, 2000 - v1
Checksum:iE834BE07CF41D9A4
GO
Isoform 2 (identifier: Q9UPV9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: MALVFQFGQP...QIEETLKYFL → MSLRDKGGEE...HDTQDLLEEV
     655-744: AHHPGKCMSQ...STSLGLVWLL → GERSQARVTV...LSVASARLCG
     745-953: Missing.

Show »
Length:686
Mass (Da):77,256
Checksum:iE14EC7B6B460E042
GO
Isoform 3 (identifier: Q9UPV9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: MALVFQFGQP...IEETLKYFLL → MQKFIEADYYELDWYYEECSDVL
     583-630: SATLHHWQQL...EVYCLNDFEE → DHAGPRPLSV...SHPRCWFEFL
     631-953: Missing.

Note: No experimental confirmation available.

Show »
Length:556
Mass (Da):63,642
Checksum:i9837FDB279C91758
GO

Sequence cautioni

The sequence BAA82994.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti503 – 5031S → P in CAH56169. (PubMed:17974005)Curated
Sequence conflicti638 – 6381S → F in CAH56169. (PubMed:17974005)Curated
Isoform 2 (identifier: Q9UPV9-2)
Sequence conflicti631 – 6311E → EE in CAH56169. (PubMed:17974005)Curated
Sequence conflicti631 – 6311E → EEE in AAH15922. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9797MALVF…KYFLL → MQKFIEADYYELDWYYEECS DVL in isoform 3. 1 PublicationVSP_045558Add
BLAST
Alternative sequencei1 – 9696MALVF…LKYFL → MSLRDKGGEEECFEYDCQDE ERKPTHRQHDTQDLLEEV in isoform 2. 2 PublicationsVSP_010839Add
BLAST
Alternative sequencei583 – 63048SATLH…NDFEE → DHAGPRPLSVLLGDSLWSLI HLRKAGHLCHAYSFFFRDSH PRCWFEFL in isoform 3. 1 PublicationVSP_045559Add
BLAST
Alternative sequencei631 – 953323Missing in isoform 3. 1 PublicationVSP_045560Add
BLAST
Alternative sequencei655 – 74490AHHPG…LVWLL → GERSQARVTVSGSRSYPSRP QASPEEMQEPPAATEEEEEE EEEEGSGEGTTISPVNLAPF PEAEFWAILTSVPGTIRSGS LSVASARLCG in isoform 2. 2 PublicationsVSP_010840Add
BLAST
Alternative sequencei745 – 953209Missing in isoform 2. 2 PublicationsVSP_010841Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028965 mRNA. Translation: BAA82994.2. Different initiation.
BX647199 mRNA. Translation: CAH56169.1.
AL713787 mRNA. Translation: CAH56394.1.
AC018358 Genomic DNA. No translation available.
AC093414 Genomic DNA. No translation available.
AC137935 Genomic DNA. No translation available.
BC015922 mRNA. Translation: AAH15922.1.
CCDSiCCDS2695.1. [Q9UPV9-2]
CCDS43072.1. [Q9UPV9-1]
CCDS58826.1. [Q9UPV9-3]
RefSeqiNP_001036111.1. NM_001042646.2. [Q9UPV9-1]
NP_001252537.1. NM_001265608.1.
NP_001252539.1. NM_001265610.1. [Q9UPV9-3]
NP_055780.2. NM_014965.4. [Q9UPV9-2]
UniGeneiHs.535711.

Genome annotation databases

EnsembliENST00000327628; ENSP00000328998; ENSG00000182606. [Q9UPV9-1]
ENST00000341421; ENSP00000340702; ENSG00000182606. [Q9UPV9-2]
ENST00000449246; ENSP00000410717; ENSG00000182606. [Q9UPV9-3]
GeneIDi22906.
KEGGihsa:22906.
UCSCiuc003cky.4. human. [Q9UPV9-1]

Polymorphism databases

DMDMi13124654.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028965 mRNA. Translation: BAA82994.2. Different initiation.
BX647199 mRNA. Translation: CAH56169.1.
AL713787 mRNA. Translation: CAH56394.1.
AC018358 Genomic DNA. No translation available.
AC093414 Genomic DNA. No translation available.
AC137935 Genomic DNA. No translation available.
BC015922 mRNA. Translation: AAH15922.1.
CCDSiCCDS2695.1. [Q9UPV9-2]
CCDS43072.1. [Q9UPV9-1]
CCDS58826.1. [Q9UPV9-3]
RefSeqiNP_001036111.1. NM_001042646.2. [Q9UPV9-1]
NP_001252537.1. NM_001265608.1.
NP_001252539.1. NM_001265610.1. [Q9UPV9-3]
NP_055780.2. NM_014965.4. [Q9UPV9-2]
UniGeneiHs.535711.

3D structure databases

ProteinModelPortaliQ9UPV9.
SMRiQ9UPV9. Positions 88-113, 315-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116570. 7 interactions.
IntActiQ9UPV9. 8 interactions.
MINTiMINT-1195439.
STRINGi9606.ENSP00000328998.

PTM databases

PhosphoSiteiQ9UPV9.

Polymorphism databases

DMDMi13124654.

Proteomic databases

MaxQBiQ9UPV9.
PaxDbiQ9UPV9.
PRIDEiQ9UPV9.

Protocols and materials databases

DNASUi22906.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327628; ENSP00000328998; ENSG00000182606. [Q9UPV9-1]
ENST00000341421; ENSP00000340702; ENSG00000182606. [Q9UPV9-2]
ENST00000449246; ENSP00000410717; ENSG00000182606. [Q9UPV9-3]
GeneIDi22906.
KEGGihsa:22906.
UCSCiuc003cky.4. human. [Q9UPV9-1]

Organism-specific databases

CTDi22906.
GeneCardsiGC03P042061.
HGNCiHGNC:29947. TRAK1.
HPAiHPA005853.
MIMi608112. gene.
neXtProtiNX_Q9UPV9.
PharmGKBiPA128394593.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG246318.
GeneTreeiENSGT00390000003132.
HOGENOMiHOG000143414.
HOVERGENiHBG069248.
InParanoidiQ9UPV9.
KOiK15369.
OMAiACGSTSH.
OrthoDBiEOG7CRTSM.
PhylomeDBiQ9UPV9.
TreeFamiTF323495.

Miscellaneous databases

ChiTaRSiTRAK1. human.
GeneWikiiTRAK1.
GenomeRNAii22906.
NextBioi35534989.
PROiQ9UPV9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UPV9.
CleanExiHS_TRAK1.
ExpressionAtlasiQ9UPV9. baseline and differential.
GenevestigatoriQ9UPV9.

Family and domain databases

InterProiIPR006933. HAP1_N.
IPR022154. Traffickng_kinesin-bd_prot_dom.
[Graphical view]
PfamiPF04849. HAP1_N. 1 hit.
PF12448. Milton. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-508 (ISOFORM 3).
    Tissue: Kidney and Salivary gland.
  3. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Kidney.
  5. "Identification and cloning of a novel family of coiled-coil domain proteins that interact with O-GlcNAc transferase."
    Iyer S.P.N., Akimoto Y., Hart G.W.
    J. Biol. Chem. 278:5399-5409(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH O-GLCNAC TRANSFERASE, GLYCOSYLATION.
  6. "GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin."
    Brickley K., Smith M.J., Beck M., Stephenson F.A.
    J. Biol. Chem. 280:14723-14732(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KIF5C, SUBCELLULAR LOCATION.
  7. "The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking."
    Fransson S., Ruusala A., Aspenstroem P.
    Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RHOT1 AND RHOT2.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: a novel cancer biomarker."
    Zhang F., Ren G., Lu Y., Jin B., Wang J., Chen X., Liu Z., Li K., Nie Y., Wang X., Fan D.
    Cancer Lett. 274:250-258(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MARKER FOR GASTRIC CANCER.
  10. "Hypertonia-associated protein Trak1 is a novel regulator of endosome-to-lysosome trafficking."
    Webber E., Li L., Chin L.S.
    J. Mol. Biol. 382:638-651(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HGS.
  11. "HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport."
    Li Y., Lim S., Hoffman D., Aspenstrom P., Federoff H.J., Rempe D.A.
    J. Cell Biol. 185:1065-1081(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTRAK1_HUMAN
AccessioniPrimary (citable) accession number: Q9UPV9
Secondary accession number(s): E9PDS2
, J3KNT7, Q63HR0, Q659B5, Q96B69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Over-expressed in all investigated carcinomas, especially in gastric adenocarcinoma and signet-ring carcinoma and may serve as a marker of gastric cancer.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.