Q9UPV9 (TRAK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking kinesin-binding protein 1

Alternative name(s):
106 kDa O-GlcNAc transferase-interacting protein

Gene names

Name:	TRAK1
Synonyms:	KIAA1042, OIP106

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors. Ref.10
Subunit structure	Interacts with O-GlcNAc transferase. Interacts with RHOT1/Miro-1 and RHOT2/Miro-2. Interacts with HGS. Interacts with GABRA1 By similarity. Interacts with KIF5C. Ref.5 Ref.6 Ref.7 Ref.10
Subcellular location	Cytoplasm. Nucleus. Mitochondrion. Early endosome. Endosome. Note: Predominantly associated with early endosome. The localization to early endosomes depends on its interaction with HRS. Colocalizes with MGARP at the mitochondria. Ref.5 Ref.6 Ref.7 Ref.9 Ref.10 Ref.11
Tissue specificity	High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined. Ref.9
Domain	The C-terminal region is required for the early endosomal and mitochondrial localization.
Post-translational modification	O-glycosylated. Ref.5
Miscellaneous	Over-expressed in all investigated carcinomas, especially in gastric adenocarcinoma and signet-ring carcinoma and may serve as a marker of gastric cancer.
Sequence similarities	Contains 1 HAP1 N-terminal domain.
Sequence caution	The sequence BAA82994.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cytoplasm Endosome Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endosome to lysosome transport Inferred from direct assay Ref.10. Source: UniProtKB protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB protein targeting Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	early endosome Inferred from direct assay Ref.10. Source: UniProtKB mitochondrion Inferred from direct assay Ref.10Ref.11. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RHOT1	Q8IXI2	3	EBI-1105048,EBI-1396430

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UPV9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UPV9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: MALVFQFGQP...QIEETLKYFL → MSLRDKGGEE...HDTQDLLEEV
     655-746: AHHPGKCMSQ...SLGLVWLLKE → GERSQARVTV...LSVASARLCG
     747-953: Missing.

Isoform 3 (identifier: Q9UPV9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: MALVFQFGQP...IEETLKYFLL → MQKFIEADYYELDWYYEECSDVL
     583-630: SATLHHWQQL...EVYCLNDFEE → DHAGPRPLSV...SHPRCWFEFL
     631-953: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Trafficking kinesin-binding protein 1		PRO_0000058037
Regions
	Domain	47 – 354	308	HAP1 N-terminal
	Region	359 – 509	151	Interaction with HGS
	Coiled coil	104 – 356	253	Potential
	Coiled coil	492 – 532	41	Potential
	Compositional bias	200 – 203	4	Poly-Ser
Amino acid modifications
	Modified residue	200	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 97	97	MALVF…KYFLL → MQKFIEADYYELDWYYEECS DVL in isoform 3.		VSP_045558
	Alternative sequence	1 – 96	96	MALVF…LKYFL → MSLRDKGGEEECFEYDCQDE ERKPTHRQHDTQDLLEEV in isoform 2.		VSP_010839
	Alternative sequence	583 – 630	48	SATLH…NDFEE → DHAGPRPLSVLLGDSLWSLI HLRKAGHLCHAYSFFFRDSH PRCWFEFL in isoform 3.		VSP_045559
	Alternative sequence	631 – 953	323	Missing in isoform 3.		VSP_045560
	Alternative sequence	655 – 746	92	AHHPG…WLLKE → GERSQARVTVSGSRSYPSRP QASPEEMQEPPAATEEEEEE EEEEEEGSGEGTTISPVNLA PFPEAEFWAILTSVPGTIRS GSLSVASARLCG in isoform 2.		VSP_010840
	Alternative sequence	747 – 953	207	Missing in isoform 2.		VSP_010841
Experimental info
	Sequence conflict	503	1	S → P in CAH56169. Ref.2
	Sequence conflict	638	1	S → F in CAH56169. Ref.2
Isoform 2:
	Sequence conflict	630 – 631	2	TE → T in CAH56169. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E834BE07CF41D9A4	FASTA	953	106,040
	10 20 30 40 50 60 MALVFQFGQP VRAQPLPGLC HGKLIRTNAC DVCNSTDLPE VEIISLLEEQ LPHYKLRADT 70 80 90 100 110 120 IYGYDHDDWL HTPLISPDAN IDLTTEQIEE TLKYFLLCAE RVGQMTKTYN DIDAVTRLLE 130 140 150 160 170 180 EKERDLELAA RIGQSLLKKN KTLTERNELL EEQVEHIREE VSQLRHELSM KDELLQFYTS 190 200 210 220 230 240 AAEESEPESV CSTPLKRNES SSSVQNYFHL DSLQKKLKDL EEENVVLRSE ASQLKTETIT 250 260 270 280 290 300 YEEKEQQLVN DCVKELRDAN VQIASISEEL AKKTEDAARQ QEEITHLLSQ IVDLQKKAKA 310 320 330 340 350 360 CAVENEELVQ HLGAAKDAQR QLTAELRELE DKYAECMEML HEAQEELKNL RNKTMPNTTS 370 380 390 400 410 420 RRYHSLGLFP MDSLAAEIEG TMRKELQLEE AESPDITHQK RVFETVRNIN QVVKQRSLTP 430 440 450 460 470 480 SPMNIPGSNQ SSAMNSLLSS CVSTPRSSFY GSDIGNVVLD NKTNSIILET EAADLGNDER 490 500 510 520 530 540 SKKPGTPGTP GSHDLETALR RLSLRRENYL SERRFFEEEQ ERKLQELAEK GELRSGSLTP 550 560 570 580 590 600 TESIMSLGTH SRFSEFTGFS GMSFSSRSYL PEKLQIVKPL EGSATLHHWQ QLAQPHLGGI 610 620 630 640 650 660 LDPRPGVVTK GFRTLDVDLD EVYCLNDFEE DDTGDHISLP RLATSTPVQH PETSAHHPGK 670 680 690 700 710 720 CMSQTNSTFT FTTCRILHPS DELTRVTPSL NSAPTPACGS TSHLKSTPVA TPCTPRRLSL 730 740 750 760 770 780 AESFTNTRES TTTMSTSLGL VWLLKERGIS AAVYDPQSWD RAGRGSLLHS YTPKMAVIPS 790 800 810 820 830 840 TPPNSPMQTP TSSPPSFEFK CTSPPYDNFL ASKPASSILR EVREKNVRSS ESQTDVSVSN 850 860 870 880 890 900 LNLVDKVRRF GVAKVVNSGR AHVPTLTEEQ GPLLCGPPGP APALVPRGLV PEGLPLRCPT 910 920 930 940 950 VTSAIGGLQL NSGIRRNRSF PTMVGSSMQM KAPVTLTSGI LMGAKLSKQT SLR
	Isoform 2 [UniParc]. Checksum: 15E9A169D2799F68	FASTA	688	77,514
	10 20 30 40 50 60 MSLRDKGGEE ECFEYDCQDE ERKPTHRQHD TQDLLEEVLC AERVGQMTKT YNDIDAVTRL 70 80 90 100 110 120 LEEKERDLEL AARIGQSLLK KNKTLTERNE LLEEQVEHIR EEVSQLRHEL SMKDELLQFY 130 140 150 160 170 180 TSAAEESEPE SVCSTPLKRN ESSSSVQNYF HLDSLQKKLK DLEEENVVLR SEASQLKTET 190 200 210 220 230 240 ITYEEKEQQL VNDCVKELRD ANVQIASISE ELAKKTEDAA RQQEEITHLL SQIVDLQKKA 250 260 270 280 290 300 KACAVENEEL VQHLGAAKDA QRQLTAELRE LEDKYAECME MLHEAQEELK NLRNKTMPNT 310 320 330 340 350 360 TSRRYHSLGL FPMDSLAAEI EGTMRKELQL EEAESPDITH QKRVFETVRN INQVVKQRSL 370 380 390 400 410 420 TPSPMNIPGS NQSSAMNSLL SSCVSTPRSS FYGSDIGNVV LDNKTNSIIL ETEAADLGND 430 440 450 460 470 480 ERSKKPGTPG TPGSHDLETA LRRLSLRREN YLSERRFFEE EQERKLQELA EKGELRSGSL 490 500 510 520 530 540 TPTESIMSLG THSRFSEFTG FSGMSFSSRS YLPEKLQIVK PLEGSATLHH WQQLAQPHLG 550 560 570 580 590 600 GILDPRPGVV TKGFRTLDVD LDEVYCLNDF EEDDTGDHIS LPRLATSTPV QHPETSGERS 610 620 630 640 650 660 QARVTVSGSR SYPSRPQASP EEMQEPPAAT EEEEEEEEEE EEGSGEGTTI SPVNLAPFPE 670 680 AEFWAILTSV PGTIRSGSLS VASARLCG
	Isoform 3 [UniParc]. Checksum: 9837FDB279C91758	FASTA	556	63,642
	10 20 30 40 50 60 MQKFIEADYY ELDWYYEECS DVLCAERVGQ MTKTYNDIDA VTRLLEEKER DLELAARIGQ 70 80 90 100 110 120 SLLKKNKTLT ERNELLEEQV EHIREEVSQL RHELSMKDEL LQFYTSAAEE SEPESVCSTP 130 140 150 160 170 180 LKRNESSSSV QNYFHLDSLQ KKLKDLEEEN VVLRSEASQL KTETITYEEK EQQLVNDCVK 190 200 210 220 230 240 ELRDANVQIA SISEELAKKT EDAARQQEEI THLLSQIVDL QKKAKACAVE NEELVQHLGA 250 260 270 280 290 300 AKDAQRQLTA ELRELEDKYA ECMEMLHEAQ EELKNLRNKT MPNTTSRRYH SLGLFPMDSL 310 320 330 340 350 360 AAEIEGTMRK ELQLEEAESP DITHQKRVFE TVRNINQVVK QRSLTPSPMN IPGSNQSSAM 370 380 390 400 410 420 NSLLSSCVST PRSSFYGSDI GNVVLDNKTN SIILETEAAD LGNDERSKKP GTPGTPGSHD 430 440 450 460 470 480 LETALRRLSL RRENYLSERR FFEEEQERKL QELAEKGELR SGSLTPTESI MSLGTHSRFS 490 500 510 520 530 540 EFTGFSGMSF SSRSYLPEKL QIVKPLEGDH AGPRPLSVLL GDSLWSLIHL RKAGHLCHAY 550 SFFFRDSHPR CWFEFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-508 (ISOFORM 3). Tissue: Kidney and Salivary gland.
[3]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Kidney.
[5]	"Identification and cloning of a novel family of coiled-coil domain proteins that interact with O-GlcNAc transferase." Iyer S.P.N., Akimoto Y., Hart G.W. J. Biol. Chem. 278:5399-5409(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH O-GLCNAC TRANSFERASE, GLYCOSYLATION.
[6]	"GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin." Brickley K., Smith M.J., Beck M., Stephenson F.A. J. Biol. Chem. 280:14723-14732(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KIF5C, SUBCELLULAR LOCATION.
[7]	"The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking." Fransson S., Ruusala A., Aspenstroem P. Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RHOT1 AND RHOT2.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: a novel cancer biomarker." Zhang F., Ren G., Lu Y., Jin B., Wang J., Chen X., Liu Z., Li K., Nie Y., Wang X., Fan D. Cancer Lett. 274:250-258(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MARKER FOR GASTRIC CANCER.
[10]	"Hypertonia-associated protein Trak1 is a novel regulator of endosome-to-lysosome trafficking." Webber E., Li L., Chin L.S. J. Mol. Biol. 382:638-651(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HGS.
[11]	"HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport." Li Y., Lim S., Hoffman D., Aspenstrom P., Federoff H.J., Rempe D.A. J. Cell Biol. 185:1065-1081(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028965 mRNA. Translation: BAA82994.2. Different initiation. BX647199 mRNA. Translation: CAH56169.1. AL713787 mRNA. Translation: CAH56394.1. AC018358 Genomic DNA. No translation available. AC093414 Genomic DNA. No translation available. AC137935 Genomic DNA. No translation available. BC015922 mRNA. Translation: AAH15922.1.
IPI	IPI00444592. IPI00444595. IPI00927380.
RefSeq	NP_001036111.1. NM_001042646.2. NP_001252537.1. NM_001265608.1. NP_001252539.1. NM_001265610.1. NP_055780.2. NM_014965.4.
UniGene	Hs.535711.
3D structure databases
ProteinModelPortal	Q9UPV9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UPV9. 6 interactions.
MINT	MINT-1195439.
STRING	9606.ENSP00000328998.
PTM databases
PhosphoSite	Q9UPV9.
Polymorphism databases
DMDM	13124654.
Proteomic databases
PaxDb	Q9UPV9.
PRIDE	Q9UPV9.
Protocols and materials databases
DNASU	22906.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327628; ENSP00000328998; ENSG00000182606. ENST00000449246; ENSP00000410717; ENSG00000182606.
GeneID	22906.
KEGG	hsa:22906.
UCSC	uc003cky.3. human. uc003ckz.4. human.
Organism-specific databases
CTD	22906.
GeneCards	GC03P042108.
HGNC	HGNC:29947. TRAK1.
HPA	HPA005853.
MIM	608112. gene.
neXtProt	NX_Q9UPV9.
PharmGKB	PA128394593.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG246318.
HOGENOM	HOG000143414.
HOVERGEN	HBG069248.
InParanoid	Q9UPV9.
KO	K15369.
OMA	NSIILEA.
OrthoDB	EOG4NGGM8.
PhylomeDB	Q9UPV9.
Gene expression databases
ArrayExpress	Q9UPV9.
Bgee	Q9UPV9.
CleanEx	HS_TRAK1.
Genevestigator	Q9UPV9.
GermOnline	ENSG00000182606. Homo sapiens.
Family and domain databases
InterPro	IPR006933. HAP1_N. IPR022154. Traffickng_kinesin-bd_prot_dom. [Graphical view]
Pfam	PF04849. HAP1_N. 1 hit. PF12448. Milton. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TRAK1. human.
GenomeRNAi	22906.
NextBio	43565.
SOURCE	Search...

Entry information

Entry name

TRAK1_HUMAN

Accession

Primary (citable) accession number: Q9UPV9
Secondary accession number(s): E9PDS2 , Q63HR0, Q659B5, Q96B69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.