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Q9UPV9

- TRAK1_HUMAN

UniProt

Q9UPV9 - TRAK1_HUMAN

Protein

Trafficking kinesin-binding protein 1

Gene

TRAK1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. endosome to lysosome transport Source: UniProtKB
    2. protein O-linked glycosylation Source: UniProtKB
    3. protein targeting Source: UniProtKB
    4. regulation of transcription from RNA polymerase II promoter Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trafficking kinesin-binding protein 1
    Alternative name(s):
    106 kDa O-GlcNAc transferase-interacting protein
    Gene namesi
    Name:TRAK1
    Synonyms:KIAA1042, OIP106
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:29947. TRAK1.

    Subcellular locationi

    Cytoplasm. Nucleus. Mitochondrion. Early endosome. Endosome
    Note: Predominantly associated with early endosome. The localization to early endosomes depends on its interaction with HRS. Colocalizes with MGARP at the mitochondria.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. early endosome Source: UniProtKB
    3. mitochondrion Source: UniProtKB
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Mitochondrion, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA128394593.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Trafficking kinesin-binding protein 1PRO_0000058037Add
    BLAST

    Post-translational modificationi

    O-glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9UPV9.
    PaxDbiQ9UPV9.
    PRIDEiQ9UPV9.

    PTM databases

    PhosphoSiteiQ9UPV9.

    Expressioni

    Tissue specificityi

    High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined.1 Publication

    Gene expression databases

    ArrayExpressiQ9UPV9.
    BgeeiQ9UPV9.
    CleanExiHS_TRAK1.
    GenevestigatoriQ9UPV9.

    Organism-specific databases

    HPAiHPA005853.

    Interactioni

    Subunit structurei

    Interacts with O-GlcNAc transferase. Interacts with RHOT1/Miro-1 and RHOT2/Miro-2. Interacts with HGS. Interacts with GABRA1 By similarity. Interacts with KIF5C.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RHOT1Q8IXI23EBI-1105048,EBI-1396430

    Protein-protein interaction databases

    BioGridi116570. 7 interactions.
    IntActiQ9UPV9. 8 interactions.
    MINTiMINT-1195439.
    STRINGi9606.ENSP00000328998.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UPV9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 354308HAP1 N-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni359 – 509151Interaction with HGSAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili104 – 356253Sequence AnalysisAdd
    BLAST
    Coiled coili492 – 53241Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi200 – 2034Poly-Ser

    Domaini

    The C-terminal region is required for the early endosomal and mitochondrial localization.

    Sequence similaritiesi

    Contains 1 HAP1 N-terminal domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG246318.
    HOGENOMiHOG000143414.
    HOVERGENiHBG069248.
    InParanoidiQ9UPV9.
    KOiK15369.
    OMAiNSCDVCN.
    OrthoDBiEOG7CRTSM.
    PhylomeDBiQ9UPV9.
    TreeFamiTF323495.

    Family and domain databases

    InterProiIPR006933. HAP1_N.
    IPR022154. Traffickng_kinesin-bd_prot_dom.
    [Graphical view]
    PfamiPF04849. HAP1_N. 1 hit.
    PF12448. Milton. 2 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9UPV9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALVFQFGQP VRAQPLPGLC HGKLIRTNAC DVCNSTDLPE VEIISLLEEQ    50
    LPHYKLRADT IYGYDHDDWL HTPLISPDAN IDLTTEQIEE TLKYFLLCAE 100
    RVGQMTKTYN DIDAVTRLLE EKERDLELAA RIGQSLLKKN KTLTERNELL 150
    EEQVEHIREE VSQLRHELSM KDELLQFYTS AAEESEPESV CSTPLKRNES 200
    SSSVQNYFHL DSLQKKLKDL EEENVVLRSE ASQLKTETIT YEEKEQQLVN 250
    DCVKELRDAN VQIASISEEL AKKTEDAARQ QEEITHLLSQ IVDLQKKAKA 300
    CAVENEELVQ HLGAAKDAQR QLTAELRELE DKYAECMEML HEAQEELKNL 350
    RNKTMPNTTS RRYHSLGLFP MDSLAAEIEG TMRKELQLEE AESPDITHQK 400
    RVFETVRNIN QVVKQRSLTP SPMNIPGSNQ SSAMNSLLSS CVSTPRSSFY 450
    GSDIGNVVLD NKTNSIILET EAADLGNDER SKKPGTPGTP GSHDLETALR 500
    RLSLRRENYL SERRFFEEEQ ERKLQELAEK GELRSGSLTP TESIMSLGTH 550
    SRFSEFTGFS GMSFSSRSYL PEKLQIVKPL EGSATLHHWQ QLAQPHLGGI 600
    LDPRPGVVTK GFRTLDVDLD EVYCLNDFEE DDTGDHISLP RLATSTPVQH 650
    PETSAHHPGK CMSQTNSTFT FTTCRILHPS DELTRVTPSL NSAPTPACGS 700
    TSHLKSTPVA TPCTPRRLSL AESFTNTRES TTTMSTSLGL VWLLKERGIS 750
    AAVYDPQSWD RAGRGSLLHS YTPKMAVIPS TPPNSPMQTP TSSPPSFEFK 800
    CTSPPYDNFL ASKPASSILR EVREKNVRSS ESQTDVSVSN LNLVDKVRRF 850
    GVAKVVNSGR AHVPTLTEEQ GPLLCGPPGP APALVPRGLV PEGLPLRCPT 900
    VTSAIGGLQL NSGIRRNRSF PTMVGSSMQM KAPVTLTSGI LMGAKLSKQT 950
    SLR 953
    Length:953
    Mass (Da):106,040
    Last modified:May 1, 2000 - v1
    Checksum:iE834BE07CF41D9A4
    GO
    Isoform 2 (identifier: Q9UPV9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-96: MALVFQFGQP...QIEETLKYFL → MSLRDKGGEE...HDTQDLLEEV
         655-744: AHHPGKCMSQ...STSLGLVWLL → GERSQARVTV...LSVASARLCG
         745-953: Missing.

    Show »
    Length:686
    Mass (Da):77,256
    Checksum:iE14EC7B6B460E042
    GO
    Isoform 3 (identifier: Q9UPV9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-97: MALVFQFGQP...IEETLKYFLL → MQKFIEADYYELDWYYEECSDVL
         583-630: SATLHHWQQL...EVYCLNDFEE → DHAGPRPLSV...SHPRCWFEFL
         631-953: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:556
    Mass (Da):63,642
    Checksum:i9837FDB279C91758
    GO

    Sequence cautioni

    The sequence BAA82994.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti503 – 5031S → P in CAH56169. (PubMed:17974005)Curated
    Sequence conflicti638 – 6381S → F in CAH56169. (PubMed:17974005)Curated
    Isoform 2 (identifier: Q9UPV9-2)
    Sequence conflicti631 – 6311E → EE in CAH56169. (PubMed:17974005)Curated
    Sequence conflicti631 – 6311E → EEE in AAH15922. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9797MALVF…KYFLL → MQKFIEADYYELDWYYEECS DVL in isoform 3. 1 PublicationVSP_045558Add
    BLAST
    Alternative sequencei1 – 9696MALVF…LKYFL → MSLRDKGGEEECFEYDCQDE ERKPTHRQHDTQDLLEEV in isoform 2. 2 PublicationsVSP_010839Add
    BLAST
    Alternative sequencei583 – 63048SATLH…NDFEE → DHAGPRPLSVLLGDSLWSLI HLRKAGHLCHAYSFFFRDSH PRCWFEFL in isoform 3. 1 PublicationVSP_045559Add
    BLAST
    Alternative sequencei631 – 953323Missing in isoform 3. 1 PublicationVSP_045560Add
    BLAST
    Alternative sequencei655 – 74490AHHPG…LVWLL → GERSQARVTVSGSRSYPSRP QASPEEMQEPPAATEEEEEE EEEEGSGEGTTISPVNLAPF PEAEFWAILTSVPGTIRSGS LSVASARLCG in isoform 2. 2 PublicationsVSP_010840Add
    BLAST
    Alternative sequencei745 – 953209Missing in isoform 2. 2 PublicationsVSP_010841Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028965 mRNA. Translation: BAA82994.2. Different initiation.
    BX647199 mRNA. Translation: CAH56169.1.
    AL713787 mRNA. Translation: CAH56394.1.
    AC018358 Genomic DNA. No translation available.
    AC093414 Genomic DNA. No translation available.
    AC137935 Genomic DNA. No translation available.
    BC015922 mRNA. Translation: AAH15922.1.
    CCDSiCCDS2695.1. [Q9UPV9-2]
    CCDS43072.1. [Q9UPV9-1]
    CCDS58826.1. [Q9UPV9-3]
    RefSeqiNP_001036111.1. NM_001042646.2. [Q9UPV9-1]
    NP_001252537.1. NM_001265608.1.
    NP_001252539.1. NM_001265610.1. [Q9UPV9-3]
    NP_055780.2. NM_014965.4. [Q9UPV9-2]
    UniGeneiHs.535711.

    Genome annotation databases

    EnsembliENST00000327628; ENSP00000328998; ENSG00000182606. [Q9UPV9-1]
    ENST00000341421; ENSP00000340702; ENSG00000182606. [Q9UPV9-2]
    ENST00000449246; ENSP00000410717; ENSG00000182606. [Q9UPV9-3]
    GeneIDi22906.
    KEGGihsa:22906.
    UCSCiuc003cky.4. human. [Q9UPV9-1]

    Polymorphism databases

    DMDMi13124654.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028965 mRNA. Translation: BAA82994.2 . Different initiation.
    BX647199 mRNA. Translation: CAH56169.1 .
    AL713787 mRNA. Translation: CAH56394.1 .
    AC018358 Genomic DNA. No translation available.
    AC093414 Genomic DNA. No translation available.
    AC137935 Genomic DNA. No translation available.
    BC015922 mRNA. Translation: AAH15922.1 .
    CCDSi CCDS2695.1. [Q9UPV9-2 ]
    CCDS43072.1. [Q9UPV9-1 ]
    CCDS58826.1. [Q9UPV9-3 ]
    RefSeqi NP_001036111.1. NM_001042646.2. [Q9UPV9-1 ]
    NP_001252537.1. NM_001265608.1.
    NP_001252539.1. NM_001265610.1. [Q9UPV9-3 ]
    NP_055780.2. NM_014965.4. [Q9UPV9-2 ]
    UniGenei Hs.535711.

    3D structure databases

    ProteinModelPortali Q9UPV9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116570. 7 interactions.
    IntActi Q9UPV9. 8 interactions.
    MINTi MINT-1195439.
    STRINGi 9606.ENSP00000328998.

    PTM databases

    PhosphoSitei Q9UPV9.

    Polymorphism databases

    DMDMi 13124654.

    Proteomic databases

    MaxQBi Q9UPV9.
    PaxDbi Q9UPV9.
    PRIDEi Q9UPV9.

    Protocols and materials databases

    DNASUi 22906.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000327628 ; ENSP00000328998 ; ENSG00000182606 . [Q9UPV9-1 ]
    ENST00000341421 ; ENSP00000340702 ; ENSG00000182606 . [Q9UPV9-2 ]
    ENST00000449246 ; ENSP00000410717 ; ENSG00000182606 . [Q9UPV9-3 ]
    GeneIDi 22906.
    KEGGi hsa:22906.
    UCSCi uc003cky.4. human. [Q9UPV9-1 ]

    Organism-specific databases

    CTDi 22906.
    GeneCardsi GC03P042108.
    HGNCi HGNC:29947. TRAK1.
    HPAi HPA005853.
    MIMi 608112. gene.
    neXtProti NX_Q9UPV9.
    PharmGKBi PA128394593.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG246318.
    HOGENOMi HOG000143414.
    HOVERGENi HBG069248.
    InParanoidi Q9UPV9.
    KOi K15369.
    OMAi NSCDVCN.
    OrthoDBi EOG7CRTSM.
    PhylomeDBi Q9UPV9.
    TreeFami TF323495.

    Miscellaneous databases

    ChiTaRSi TRAK1. human.
    GeneWikii TRAK1.
    GenomeRNAii 22906.
    NextBioi 35534989.
    PROi Q9UPV9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9UPV9.
    Bgeei Q9UPV9.
    CleanExi HS_TRAK1.
    Genevestigatori Q9UPV9.

    Family and domain databases

    InterProi IPR006933. HAP1_N.
    IPR022154. Traffickng_kinesin-bd_prot_dom.
    [Graphical view ]
    Pfami PF04849. HAP1_N. 1 hit.
    PF12448. Milton. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-508 (ISOFORM 3).
      Tissue: Kidney and Salivary gland.
    3. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Kidney.
    5. "Identification and cloning of a novel family of coiled-coil domain proteins that interact with O-GlcNAc transferase."
      Iyer S.P.N., Akimoto Y., Hart G.W.
      J. Biol. Chem. 278:5399-5409(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH O-GLCNAC TRANSFERASE, GLYCOSYLATION.
    6. "GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin."
      Brickley K., Smith M.J., Beck M., Stephenson F.A.
      J. Biol. Chem. 280:14723-14732(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KIF5C, SUBCELLULAR LOCATION.
    7. "The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking."
      Fransson S., Ruusala A., Aspenstroem P.
      Biochem. Biophys. Res. Commun. 344:500-510(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH RHOT1 AND RHOT2.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: a novel cancer biomarker."
      Zhang F., Ren G., Lu Y., Jin B., Wang J., Chen X., Liu Z., Li K., Nie Y., Wang X., Fan D.
      Cancer Lett. 274:250-258(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MARKER FOR GASTRIC CANCER.
    10. "Hypertonia-associated protein Trak1 is a novel regulator of endosome-to-lysosome trafficking."
      Webber E., Li L., Chin L.S.
      J. Mol. Biol. 382:638-651(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HGS.
    11. "HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport."
      Li Y., Lim S., Hoffman D., Aspenstrom P., Federoff H.J., Rempe D.A.
      J. Cell Biol. 185:1065-1081(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTRAK1_HUMAN
    AccessioniPrimary (citable) accession number: Q9UPV9
    Secondary accession number(s): E9PDS2
    , J3KNT7, Q63HR0, Q659B5, Q96B69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Over-expressed in all investigated carcinomas, especially in gastric adenocarcinoma and signet-ring carcinoma and may serve as a marker of gastric cancer.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3