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Protein

Protein Smaug homolog 1

Gene

SAMD4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a translational repressor of SRE-containing messengers.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • translation repressor activity Source: MGI

GO - Biological processi

  • negative regulation of translation Source: GOC
  • positive regulation of translation Source: MGI
  • regulation of transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Smaug homolog 1
Short name:
Smaug 1
Short name:
hSmaug1
Alternative name(s):
Sterile alpha motif domain-containing protein 4A
Short name:
SAM domain-containing protein 4A
Gene namesi
Name:SAMD4A
Synonyms:KIAA1053, SAMD4, SMAUG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:23023. SAMD4A.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell projectiondendrite By similarity
  • Cell junctionsynapsesynaptosome By similarity

  • Note: Enriched in synaptoneurosomes (By similarity). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Colocalizes throughout the cytoplasm in granules with polyadenylated RNAs, PABPC1 and STAU1. Also frequently colocalizes in cytoplasmic stress granule-like foci with ELAVL1, TIA1 and TIAL1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394596.

Polymorphism and mutation databases

BioMutaiSAMD4A.
DMDMi254763391.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Protein Smaug homolog 1PRO_0000097570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei168 – 1681PhosphoserineBy similarity
Modified residuei420 – 4201PhosphoserineCombined sources
Modified residuei424 – 4241PhosphothreonineCombined sources
Modified residuei580 – 5801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UPU9.
MaxQBiQ9UPU9.
PaxDbiQ9UPU9.
PRIDEiQ9UPU9.

PTM databases

iPTMnetiQ9UPU9.
PhosphoSiteiQ9UPU9.
SwissPalmiQ9UPU9.

Expressioni

Gene expression databases

BgeeiQ9UPU9.
CleanExiHS_SAMD4A.
ExpressionAtlasiQ9UPU9. baseline and differential.
GenevisibleiQ9UPU9. HS.

Organism-specific databases

HPAiHPA043061.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GOLGA2Q083793EBI-1047497,EBI-618309
NUTM1Q86Y263EBI-1047497,EBI-10178410

Protein-protein interaction databases

BioGridi116673. 15 interactions.
IntActiQ9UPU9. 6 interactions.
MINTiMINT-3976554.
STRINGi9606.ENSP00000350261.

Structurei

3D structure databases

ProteinModelPortaliQ9UPU9.
SMRiQ9UPU9. Positions 324-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini323 – 39169SAMAdd
BLAST

Sequence similaritiesi

Belongs to the SMAUG family.Curated

Phylogenomic databases

eggNOGiKOG3791. Eukaryota.
ENOG410XSUP. LUCA.
GeneTreeiENSGT00390000015877.
HOGENOMiHOG000290216.
InParanoidiQ9UPU9.
OMAiEAKVEYM.
OrthoDBiEOG7TTQ74.
PhylomeDBiQ9UPU9.
TreeFamiTF324165.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.170. 2 hits.
InterProiIPR015327. PHAT_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPU9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMFRDQVGVL AGWFKGWNEC EQTVALLSLL KRVSQTQARF LQLCLEHSLA
60 70 80 90 100
DCAELHVLER EANSPGIINQ WQQESKDKVI SLLLTHLPLL KPGNLDAKVE
110 120 130 140 150
YMKLLPKILA HSIEHNQHIE ESRQLLSYAL IHPATSLEDR SALAMWLNHL
160 170 180 190 200
EDRTSTSFGG QNRGRSDSVD YGQTHYYHQR QNSDDKLNGW QNSRDSGICI
210 220 230 240 250
NASNWQDKSM GCENGHVPLY SSSSVPTTIN TIGTSTSTIL SGQAHHSPLK
260 270 280 290 300
RSVSLTPPMN VPNQPLGHGW MSHEDLRARG PQCLPSDHAP LSPQSSVASS
310 320 330 340 350
GSGGSEHLED QTTARNTFQE EGSGMKDVPA WLKSLRLHKY AALFSQMTYE
360 370 380 390 400
EMMALTECQL EAQNVTKGAR HKIVISIQKL KERQNLLKSL ERDIIEGGSL
410 420 430 440 450
RIPLQELHQM ILTPIKAYSS PSTTPEARRR EPQAPRQPSL MGPESQSPDC
460 470 480 490 500
KDGAAATGAT ATPSAGASGG LQPHQLSSCD GELAVAPLPE GDLPGQFTRV
510 520 530 540 550
MGKVCTQLLV SRPDEENISS YLQLIDKCLI HEAFTETQKK RLLSWKQQVQ
560 570 580 590 600
KLFRSFPRKT LLDISGYRQQ RNRGFGQSNS LPTAGSVGGG MGRRNPRQYQ
610 620 630 640 650
IPSRNVPSAR LGLLGTSGFV SSNQRNTTAT PTIMKQGRQN LWFANPGGSN
660 670 680 690 700
SMPSRTHSSV QRTRSLPVHT SPQNMLMFQQ PEFQLPVTEP DINNRLESLC
710
LSMTEHALGD GVDRTSTI
Length:718
Mass (Da):79,415
Last modified:July 28, 2009 - v3
Checksum:i01F5D49E6447F771
GO
Isoform 2 (identifier: Q9UPU9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-101: Missing.

Show »
Length:617
Mass (Da):67,929
Checksum:i5808A07C51439A57
GO
Isoform 3 (identifier: Q9UPU9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-327: ILSGQAHHSP...FQEEGSGMKD → N

Show »
Length:630
Mass (Da):70,095
Checksum:iCDD4F9337B6ED487
GO

Sequence cautioni

The sequence AAH57838.1 differs from that shown.Intron retention.Curated
The sequence AAP97302.1 differs from that shown.Intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081I → T in AAH57838 (PubMed:15489334).Curated
Sequence conflicti138 – 1381E → K in AK024652 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 101101Missing in isoform 2. 1 PublicationVSP_037778Add
BLAST
Alternative sequencei239 – 32789ILSGQ…SGMKD → N in isoform 3. 2 PublicationsVSP_037779Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133444 Genomic DNA. No translation available.
AL138994 Genomic DNA. No translation available.
AL359792 Genomic DNA. No translation available.
AF429970 mRNA. Translation: AAP97302.1. Sequence problems.
BC057838 mRNA. Translation: AAH57838.1. Sequence problems.
BC121173 mRNA. Translation: AAI21174.1.
BC121174 mRNA. Translation: AAI21175.1.
AK024652 mRNA. No translation available.
AB028976 mRNA. Translation: BAA83005.1.
CCDSiCCDS32084.2. [Q9UPU9-1]
CCDS55917.2. [Q9UPU9-3]
RefSeqiNP_001155048.2. NM_001161576.2. [Q9UPU9-3]
NP_001155049.1. NM_001161577.1.
NP_056404.4. NM_015589.5. [Q9UPU9-1]
UniGeneiHs.733324.
Hs.98259.

Genome annotation databases

EnsembliENST00000251091; ENSP00000251091; ENSG00000020577. [Q9UPU9-3]
ENST00000392067; ENSP00000375919; ENSG00000020577. [Q9UPU9-1]
ENST00000554335; ENSP00000452535; ENSG00000020577. [Q9UPU9-1]
GeneIDi23034.
KEGGihsa:23034.
UCSCiuc001xbb.4. human. [Q9UPU9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133444 Genomic DNA. No translation available.
AL138994 Genomic DNA. No translation available.
AL359792 Genomic DNA. No translation available.
AF429970 mRNA. Translation: AAP97302.1. Sequence problems.
BC057838 mRNA. Translation: AAH57838.1. Sequence problems.
BC121173 mRNA. Translation: AAI21174.1.
BC121174 mRNA. Translation: AAI21175.1.
AK024652 mRNA. No translation available.
AB028976 mRNA. Translation: BAA83005.1.
CCDSiCCDS32084.2. [Q9UPU9-1]
CCDS55917.2. [Q9UPU9-3]
RefSeqiNP_001155048.2. NM_001161576.2. [Q9UPU9-3]
NP_001155049.1. NM_001161577.1.
NP_056404.4. NM_015589.5. [Q9UPU9-1]
UniGeneiHs.733324.
Hs.98259.

3D structure databases

ProteinModelPortaliQ9UPU9.
SMRiQ9UPU9. Positions 324-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116673. 15 interactions.
IntActiQ9UPU9. 6 interactions.
MINTiMINT-3976554.
STRINGi9606.ENSP00000350261.

PTM databases

iPTMnetiQ9UPU9.
PhosphoSiteiQ9UPU9.
SwissPalmiQ9UPU9.

Polymorphism and mutation databases

BioMutaiSAMD4A.
DMDMi254763391.

Proteomic databases

EPDiQ9UPU9.
MaxQBiQ9UPU9.
PaxDbiQ9UPU9.
PRIDEiQ9UPU9.

Protocols and materials databases

DNASUi23034.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000251091; ENSP00000251091; ENSG00000020577. [Q9UPU9-3]
ENST00000392067; ENSP00000375919; ENSG00000020577. [Q9UPU9-1]
ENST00000554335; ENSP00000452535; ENSG00000020577. [Q9UPU9-1]
GeneIDi23034.
KEGGihsa:23034.
UCSCiuc001xbb.4. human. [Q9UPU9-1]

Organism-specific databases

CTDi23034.
GeneCardsiSAMD4A.
HGNCiHGNC:23023. SAMD4A.
HPAiHPA043061.
MIMi610747. gene.
neXtProtiNX_Q9UPU9.
PharmGKBiPA128394596.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3791. Eukaryota.
ENOG410XSUP. LUCA.
GeneTreeiENSGT00390000015877.
HOGENOMiHOG000290216.
InParanoidiQ9UPU9.
OMAiEAKVEYM.
OrthoDBiEOG7TTQ74.
PhylomeDBiQ9UPU9.
TreeFamiTF324165.

Miscellaneous databases

ChiTaRSiSAMD4A. human.
GenomeRNAii23034.
PROiQ9UPU9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9UPU9.
CleanExiHS_SAMD4A.
ExpressionAtlasiQ9UPU9. baseline and differential.
GenevisibleiQ9UPU9. HS.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.170. 2 hits.
InterProiIPR015327. PHAT_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Guo J.H., Yu L.
    Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-238 (ISOFORM 2).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-238 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-718 (ISOFORM 3).
    Tissue: Placenta.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-718 (ISOFORM 1).
  5. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 123-718 (ISOFORM 3).
    Tissue: Brain.
  6. "Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules."
    Baez M.V., Boccaccio G.L.
    J. Biol. Chem. 280:43131-43140(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-420 AND THR-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiSMAG1_HUMAN
AccessioniPrimary (citable) accession number: Q9UPU9
Secondary accession number(s): A8MPZ5, Q0VA96, Q6PEW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: July 28, 2009
Last modified: June 8, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.