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Q9UPT9

- UBP22_HUMAN

UniProt

Q9UPT9 - UBP22_HUMAN

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Protein
Ubiquitin carboxyl-terminal hydrolase 22
Gene
USP22, KIAA1063, USP3L
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.3 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei185 – 1851Nucleophile By similarity
Active sitei479 – 4791Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 12161UBP-type
Add
BLAST

GO - Molecular functioni

  1. enzyme binding Source: UniProt
  2. ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. transcription coactivator activity Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. chromatin organization Source: Reactome
  3. embryo development Source: UniProtKB
  4. histone H4 acetylation Source: GOC
  5. histone deubiquitination Source: UniProtKB
  6. histone ubiquitination Source: UniProtKB
  7. positive regulation of mitotic cell cycle Source: UniProtKB
  8. positive regulation of transcription, DNA-templated Source: UniProtKB
  9. protein deubiquitination Source: UniProtKB
  10. transcription, DNA-templated Source: UniProtKB-KW
  11. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_172610. HATs acetylate histones.

Protein family/group databases

MEROPSiC19.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:USP22
Synonyms:KIAA1063, USP3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:12621. USP22.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. SAGA complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi471 – 4711H → A: Impairs its function as a positive modulator of androgen receptor transactivation; when associated with A-479. 1 Publication
Mutagenesisi479 – 4791H → A: Impairs its function as a positive modulator of androgen receptor transactivation; when associated with A-471. 1 Publication

Organism-specific databases

PharmGKBiPA37247.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Ubiquitin carboxyl-terminal hydrolase 22
PRO_0000080650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9UPT9.
PaxDbiQ9UPT9.
PRIDEiQ9UPT9.

PTM databases

PhosphoSiteiQ9UPT9.

Expressioni

Tissue specificityi

Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.1 Publication

Gene expression databases

ArrayExpressiQ9UPT9.
BgeeiQ9UPT9.
CleanExiHS_USP22.
GenevestigatoriQ9UPT9.

Organism-specific databases

HPAiHPA044980.

Interactioni

Subunit structurei

Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recritment to the SAGA complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FUBP1Q96AE43EBI-723510,EBI-711404
NFATC2Q134692EBI-723510,EBI-716258

Protein-protein interaction databases

BioGridi116914. 42 interactions.
IntActiQ9UPT9. 35 interactions.
MINTiMINT-1390273.
STRINGi9606.ENSP00000261497.

Structurei

3D structure databases

ProteinModelPortaliQ9UPT9.
SMRiQ9UPT9. Positions 76-517.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini176 – 520345USP
Add
BLAST

Sequence similaritiesi

Contains 1 USP domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000007260.
HOVERGENiHBG058014.
InParanoidiQ9UPT9.
KOiK11366.
OMAiYCQMCDD.
OrthoDBiEOG7FR7G7.
PhylomeDBiQ9UPT9.
TreeFamiTF323554.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9UPT9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVSRPEPEGE AMDAELAVAP PGCSHLGSFK VDNWKQNLRA IYQCFVWSGT    50
AEARKRKAKS CICHVCGVHL NRLHSCLYCV FFGCFTKKHI HEHAKAKRHN 100
LAIDLMYGGI YCFLCQDYIY DKDMEIIAKE EQRKAWKMQG VGEKFSTWEP 150
TKRELELLKH NPKRRKITSN CTIGLRGLIN LGNTCFMNCI VQALTHTPLL 200
RDFFLSDRHR CEMQSPSSCL VCEMSSLFQE FYSGHRSPHI PYKLLHLVWT 250
HARHLAGYEQ QDAHEFLIAA LDVLHRHCKG DDNGKKANNP NHCNCIIDQI 300
FTGGLQSDVT CQVCHGVSTT IDPFWDISLD LPGSSTPFWP LSPGSEGNVV 350
NGESHVSGTT TLTDCLRRFT RPEHLGSSAK IKCSGCHSYQ ESTKQLTMKK 400
LPIVACFHLK RFEHSAKLRR KITTYVSFPL ELDMTPFMAS SKESRMNGQY 450
QQPTDSLNND NKYSLFAVVN HQGTLESGHY TSFIRQHKDQ WFKCDDAIIT 500
KASIKDVLDS EGYLLFYHKQ FLEYE 525
Length:525
Mass (Da):59,961
Last modified:September 13, 2005 - v2
Checksum:iD18BFE84BE8E5915
GO
Isoform 2 (identifier: Q9UPT9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MVSRPEPEGE...SGTAEARKRK → MAPGWPSLSA...RATALQGPSQ

Show »
Length:513
Mass (Da):58,179
Checksum:i79C5E8674661256E
GO

Sequence cautioni

The sequence AAH25317.1 differs from that shown. Reason: Frameshift at position 355.
Isoform 2 : The sequence AAI10500.1 differs from that shown. Reason: Frameshift at position 3.
The sequence BAA83015.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5757MVSRP…ARKRK → MAPGWPSLSAGSRQEAPQLA AGGSAYQAVGRQFQPRATAL QGPSQ in isoform 2.
VSP_036720Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851F → L in AAI26899. 1 Publication
Sequence conflicti380 – 3801K → T in CAE45893. 1 Publication
Sequence conflicti496 – 4961D → V in AAH25317. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB028986 mRNA. Translation: BAA83015.1. Different initiation.
BC007196 mRNA. Translation: AAH07196.1.
BC025317 mRNA. Translation: AAH25317.1. Frameshift.
BC110499 mRNA. Translation: AAI10500.1. Frameshift.
BC126898 mRNA. Translation: AAI26899.1.
BX640815 mRNA. Translation: CAE45893.1.
CCDSiCCDS42285.1. [Q9UPT9-1]
RefSeqiNP_056091.1. NM_015276.1. [Q9UPT9-1]
UniGeneiHs.462492.

Genome annotation databases

EnsembliENST00000261497; ENSP00000261497; ENSG00000124422. [Q9UPT9-1]
ENST00000537526; ENSP00000440950; ENSG00000124422. [Q9UPT9-2]
GeneIDi23326.
KEGGihsa:23326.
UCSCiuc002gyl.4. human. [Q9UPT9-1]
uc002gyn.4. human. [Q9UPT9-2]

Polymorphism databases

DMDMi78103328.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB028986 mRNA. Translation: BAA83015.1 . Different initiation.
BC007196 mRNA. Translation: AAH07196.1 .
BC025317 mRNA. Translation: AAH25317.1 . Frameshift.
BC110499 mRNA. Translation: AAI10500.1 . Frameshift.
BC126898 mRNA. Translation: AAI26899.1 .
BX640815 mRNA. Translation: CAE45893.1 .
CCDSi CCDS42285.1. [Q9UPT9-1 ]
RefSeqi NP_056091.1. NM_015276.1. [Q9UPT9-1 ]
UniGenei Hs.462492.

3D structure databases

ProteinModelPortali Q9UPT9.
SMRi Q9UPT9. Positions 76-517.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116914. 42 interactions.
IntActi Q9UPT9. 35 interactions.
MINTi MINT-1390273.
STRINGi 9606.ENSP00000261497.

Protein family/group databases

MEROPSi C19.035.

PTM databases

PhosphoSitei Q9UPT9.

Polymorphism databases

DMDMi 78103328.

Proteomic databases

MaxQBi Q9UPT9.
PaxDbi Q9UPT9.
PRIDEi Q9UPT9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261497 ; ENSP00000261497 ; ENSG00000124422 . [Q9UPT9-1 ]
ENST00000537526 ; ENSP00000440950 ; ENSG00000124422 . [Q9UPT9-2 ]
GeneIDi 23326.
KEGGi hsa:23326.
UCSCi uc002gyl.4. human. [Q9UPT9-1 ]
uc002gyn.4. human. [Q9UPT9-2 ]

Organism-specific databases

CTDi 23326.
GeneCardsi GC17M020902.
HGNCi HGNC:12621. USP22.
HPAi HPA044980.
MIMi 612116. gene.
neXtProti NX_Q9UPT9.
PharmGKBi PA37247.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000007260.
HOVERGENi HBG058014.
InParanoidi Q9UPT9.
KOi K11366.
OMAi YCQMCDD.
OrthoDBi EOG7FR7G7.
PhylomeDBi Q9UPT9.
TreeFami TF323554.

Enzyme and pathway databases

Reactomei REACT_172610. HATs acetylate histones.

Miscellaneous databases

ChiTaRSi USP22. human.
GenomeRNAii 23326.
NextBioi 45232.
PROi Q9UPT9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9UPT9.
Bgeei Q9UPT9.
CleanExi HS_USP22.
Genevestigatori Q9UPT9.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Uterus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 73-525.
    Tissue: Colon endothelium.
  4. "The expression patterns of deubiquitinating enzymes, USP22 and Usp22."
    Lee H.-J., Kim M.-S., Shin J.-M., Park T.-J., Chung H.-M., Baek K.-H.
    Gene Expr. Patterns 6:277-284(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing."
    Zhao Y., Lang G., Ito S., Bonnet J., Metzger E., Sawatsubashi S., Suzuki E., Le Guezennec X., Stunnenberg H.G., Krasnov A., Georgieva S.G., Schuele R., Takeyama K., Kato S., Tora L., Devys D.
    Mol. Cell 29:92-101(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HISTONE DEUBIQUITINATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SAGA COMPLEX, MUTAGENESIS OF HIS-471 AND HIS-479.
  6. "The putative cancer stem cell marker USP22 is a subunit of the human SAGA complex required for activated transcription and cell-cycle progression."
    Zhang X.-Y., Varthi M., Sykes S.M., Phillips C., Warzecha C., Zhu W., Wyce A., Thorne A.W., Berger S.L., McMahon S.B.
    Mol. Cell 29:102-111(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HISTONE H2A DEUBIQUITINATION, CATALYTIC ACTIVITY.
  7. "USP22, an hSAGA subunit and potential cancer stem cell marker, reverses the polycomb-catalyzed ubiquitylation of histone H2A."
    Zhang X.-Y., Pfeiffer H.K., Thorne A.W., McMahon S.B.
    Cell Cycle 7:1522-1524(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HISTONE H2B DEUBIQUITINATION.
  8. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-129, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP22_HUMAN
AccessioniPrimary (citable) accession number: Q9UPT9
Secondary accession number(s): A0JNS3
, Q2NLE2, Q6MZY4, Q8TBS8, Q96IW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: September 13, 2005
Last modified: September 3, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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