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Protein

C-Jun-amino-terminal kinase-interacting protein 3

Gene

MAPK8IP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138834-MONOMER.
SignaLinkiQ9UPT6.
SIGNORiQ9UPT6.

Names & Taxonomyi

Protein namesi
Recommended name:
C-Jun-amino-terminal kinase-interacting protein 3
Short name:
JIP-3
Short name:
JNK-interacting protein 3
Alternative name(s):
JNK MAP kinase scaffold protein 3
Mitogen-activated protein kinase 8-interacting protein 3
Gene namesi
Name:MAPK8IP3
Synonyms:JIP3, KIAA1066
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:6884. MAPK8IP3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi23162.
OpenTargetsiENSG00000138834.
PharmGKBiPA30628.

Polymorphism and mutation databases

BioMutaiMAPK8IP3.
DMDMi145559484.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206331 – 1336C-Jun-amino-terminal kinase-interacting protein 3Add BLAST1336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265Phosphothreonine; by MAPKBy similarity1
Modified residuei275Phosphothreonine; by MAPKBy similarity1
Modified residuei286Phosphothreonine; by MAPKBy similarity1
Modified residuei314Phosphoserine; by ROCK11 Publication1
Modified residuei364Phosphoserine; by ROCK11 Publication1
Modified residuei365Phosphoserine; by ROCK11 Publication1
Modified residuei602PhosphoserineBy similarity1
Modified residuei676PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by ROCK1 is crucial for the recruitment of JNK.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UPT6.
PaxDbiQ9UPT6.
PeptideAtlasiQ9UPT6.
PRIDEiQ9UPT6.

PTM databases

iPTMnetiQ9UPT6.
PhosphoSitePlusiQ9UPT6.

Expressioni

Gene expression databases

BgeeiENSG00000138834.
CleanExiHS_MAPK8IP3.
ExpressionAtlasiQ9UPT6. baseline and differential.
GenevisibleiQ9UPT6. HS.

Organism-specific databases

HPAiCAB005580.
HPA041785.
HPA069311.

Interactioni

Subunit structurei

Forms homo- or heterooligomeric complexes. The central region of MAPK8IP3 interacts with the C-terminal of MAPK8IP2 but not MAPK8IP1. Binds specific components of the JNK signaling pathway namely MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3 to the N-terminal region, MAP2K4/MKK4 and MAP2K7/MKK7 to the central region and MAP3K11 to the C-terminal region. Binds the TPR motif-containing C-terminal of kinesin light chain, KLC1. Pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location (By similarity). Interacts with ROCK1 and this interaction is enhanced by ultraviolet-B (UVB) radiation.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LDLRAP1Q5SW964EBI-717887,EBI-747813

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116774. 34 interactors.
IntActiQ9UPT6. 9 interactors.
MINTiMINT-1404510.
STRINGi9606.ENSP00000250894.

Structurei

Secondary structure

11336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi433 – 484Combined sources52

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PXJX-ray2.06A/B/C433-486[»]
ProteinModelPortaliQ9UPT6.
SMRiQ9UPT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 100RH1PROSITE-ProRule annotationAdd BLAST89
Domaini520 – 594RH2PROSITE-ProRule annotationAdd BLAST75

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili58 – 177Sequence analysisAdd BLAST120
Coiled coili440 – 554Sequence analysisAdd BLAST115

Sequence similaritiesi

Belongs to the JIP scaffold family.Curated
Contains 1 RH1 domain.PROSITE-ProRule annotation
Contains 1 RH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2077. Eukaryota.
ENOG410XQ19. LUCA.
GeneTreeiENSGT00670000097546.
HOVERGENiHBG024110.
InParanoidiQ9UPT6.
KOiK04436.
PhylomeDBiQ9UPT6.
TreeFamiTF313096.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR032486. JIP_LZII.
IPR019143. JNK/Rab-associated_protein-1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF16471. JIP_LZII. 1 hit.
PF09744. Jnk-SapK_ap_N. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51776. RH1. 1 hit.
PS51777. RH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE
60 70 80 90 100
VVKELMPLVV NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL
110 120 130 140 150
RRQAEEKFIE FEDALEQEKK ELQIQVEHYE FQTRQLELKA KNYADQISRL
160 170 180 190 200
EERESEMKKE YNALHQRHTE MIQTYVEHIE RSKMQQVGGN SQTESSLPGR
210 220 230 240 250
RKERPTSLNV FPLADGTVRA QIGGKLVPAG DHWHLSDLGQ LQSSSSYQCP
260 270 280 290 300
QDEMSESGQS SAAATPSTTG TKSNTPTSSV PSAAVTPLNE SLQPLGDYGV
310 320 330 340 350
GSKNSKRARE KRDSRNMEVQ VTQEMRNVSI GMGSSDEWSD VQDIIDSTPE
360 370 380 390 400
LDMCPETRLD RTGSSPTQGI VNKAFGINTD SLYHELSTAG SEVIGDVDEG
410 420 430 440 450
ADLLGEFSVR DDFFGMGKEV GNLLLENSQL LETKNALNVV KNDLIAKVDQ
460 470 480 490 500
LSGEQEVLRG ELEAAKQAKV KLENRIKELE EELKRVKSEA IIARREPKEE
510 520 530 540 550
AEDVSSYLCT ESDKIPMAQR RRFTRVEMAR VLMERNQYKE RLMELQEAVR
560 570 580 590 600
WTEMIRASRE HPSVQEKKKS TIWQFFSRLF SSSSSPPPAK RPYPSVNIHY
610 620 630 640 650
KSPTTAGFSQ RRNHAMCPIS AGSRPLEFFP DDDCTSSARR EQKREQYRQV
660 670 680 690 700
REHVRNDDGR LQACGWSLPA KYKQLSPNGG QEDTRMKNVP VPVYCRPLVE
710 720 730 740 750
KDPTMKLWCA AGVNLSGWRP NEDDAGNGVK PAPGRDPLTC DREGDGEPKS
760 770 780 790 800
AHTSPEKKKA KELPEMDATS SRVWILTSTL TTSKVVIIDA NQPGTVVDQF
810 820 830 840 850
TVCNAHVLCI SSIPAASDSD YPPGEMFLDS DVNPEDPGAD GVLAGITLVG
860 870 880 890 900
CATRCNVPRS NCSSRGDTPV LDKGQGEVAT IANGKVNPSQ STEEATEATE
910 920 930 940 950
VPDPGPSEPE TATLRPGPLT EHVFTDPAPT PSSGPQPGSE NGPEPDSSST
960 970 980 990 1000
RPEPEPSGDP TGAGSSAAPT MWLGAQNGWL YVHSAVANWK KCLHSIKLKD
1010 1020 1030 1040 1050
SVLSLVHVKG RVLVALADGT LAIFHRGEDG QWDLSNYHLM DLGHPHHSIR
1060 1070 1080 1090 1100
CMAVVYDRVW CGYKNKVHVI QPKTMQIEKS FDAHPRRESQ VRQLAWIGDG
1110 1120 1130 1140 1150
VWVSIRLDST LRLYHAHTHQ HLQDVDIEPY VSKMLGTGKL GFSFVRITAL
1160 1170 1180 1190 1200
LVAGSRLWVG TGNGVVISIP LTETVVLHRG QLLGLRANKT SPTSGEGARP
1210 1220 1230 1240 1250
GGIIHVYGDD SSDRAASSFI PYCSMAQAQL CFHGHRDAVK FFVSVPGNVL
1260 1270 1280 1290 1300
ATLNGSVLDS PAEGPGPAAP ASEVEGQKLR NVLVLSGGEG YIDFRIGDGE
1310 1320 1330
DDETEEGAGD MSQVKPVLSK AERSHIIVWQ VSYTPE
Length:1,336
Mass (Da):147,457
Last modified:April 17, 2007 - v3
Checksum:i84166F5725D74B25
GO
Isoform 2 (identifier: Q9UPT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-250: CP → VL
     251-1336: Missing.

Show »
Length:250
Mass (Da):29,052
Checksum:iE7C2A9B0793A810E
GO

Sequence cautioni

The sequence AAK61290 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA83018 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB15727 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB72318 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049667753T → A.Corresponds to variant rs2294619dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024430249 – 250CP → VL in isoform 2. 1 Publication2
Alternative sequenceiVSP_024431251 – 1336Missing in isoform 2. 1 PublicationAdd BLAST1086

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028989 mRNA. Translation: BAA83018.2. Different initiation.
AK024437 mRNA. Translation: BAB15727.1. Different initiation.
AE006639 Genomic DNA. Translation: AAK61290.1. Sequence problems.
AL031718 Genomic DNA. Translation: CAB72318.1. Sequence problems.
AL031710, AC012180, AL031717 Genomic DNA. Translation: CAM26364.1.
AL031717, AC012180, AL031710 Genomic DNA. Translation: CAM26394.1.
BC137123 mRNA. Translation: AAI37124.1.
BC137124 mRNA. Translation: AAI37125.1.
BC150266 mRNA. Translation: AAI50267.1.
CCDSiCCDS10442.2. [Q9UPT6-1]
RefSeqiNP_001035529.1. NM_001040439.1.
NP_001305781.1. NM_001318852.1.
NP_055948.2. NM_015133.4. [Q9UPT6-1]
UniGeneiHs.207763.

Genome annotation databases

EnsembliENST00000250894; ENSP00000250894; ENSG00000138834. [Q9UPT6-1]
GeneIDi23162.
KEGGihsa:23162.
UCSCiuc002cmk.4. human. [Q9UPT6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028989 mRNA. Translation: BAA83018.2. Different initiation.
AK024437 mRNA. Translation: BAB15727.1. Different initiation.
AE006639 Genomic DNA. Translation: AAK61290.1. Sequence problems.
AL031718 Genomic DNA. Translation: CAB72318.1. Sequence problems.
AL031710, AC012180, AL031717 Genomic DNA. Translation: CAM26364.1.
AL031717, AC012180, AL031710 Genomic DNA. Translation: CAM26394.1.
BC137123 mRNA. Translation: AAI37124.1.
BC137124 mRNA. Translation: AAI37125.1.
BC150266 mRNA. Translation: AAI50267.1.
CCDSiCCDS10442.2. [Q9UPT6-1]
RefSeqiNP_001035529.1. NM_001040439.1.
NP_001305781.1. NM_001318852.1.
NP_055948.2. NM_015133.4. [Q9UPT6-1]
UniGeneiHs.207763.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PXJX-ray2.06A/B/C433-486[»]
ProteinModelPortaliQ9UPT6.
SMRiQ9UPT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116774. 34 interactors.
IntActiQ9UPT6. 9 interactors.
MINTiMINT-1404510.
STRINGi9606.ENSP00000250894.

PTM databases

iPTMnetiQ9UPT6.
PhosphoSitePlusiQ9UPT6.

Polymorphism and mutation databases

BioMutaiMAPK8IP3.
DMDMi145559484.

Proteomic databases

EPDiQ9UPT6.
PaxDbiQ9UPT6.
PeptideAtlasiQ9UPT6.
PRIDEiQ9UPT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000250894; ENSP00000250894; ENSG00000138834. [Q9UPT6-1]
GeneIDi23162.
KEGGihsa:23162.
UCSCiuc002cmk.4. human. [Q9UPT6-1]

Organism-specific databases

CTDi23162.
DisGeNETi23162.
GeneCardsiMAPK8IP3.
HGNCiHGNC:6884. MAPK8IP3.
HPAiCAB005580.
HPA041785.
HPA069311.
MIMi605431. gene.
neXtProtiNX_Q9UPT6.
OpenTargetsiENSG00000138834.
PharmGKBiPA30628.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2077. Eukaryota.
ENOG410XQ19. LUCA.
GeneTreeiENSGT00670000097546.
HOVERGENiHBG024110.
InParanoidiQ9UPT6.
KOiK04436.
PhylomeDBiQ9UPT6.
TreeFamiTF313096.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000138834-MONOMER.
SignaLinkiQ9UPT6.
SIGNORiQ9UPT6.

Miscellaneous databases

ChiTaRSiMAPK8IP3. human.
GeneWikiiMAPK8IP3.
GenomeRNAii23162.
PROiQ9UPT6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138834.
CleanExiHS_MAPK8IP3.
ExpressionAtlasiQ9UPT6. baseline and differential.
GenevisibleiQ9UPT6. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR032486. JIP_LZII.
IPR019143. JNK/Rab-associated_protein-1_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF16471. JIP_LZII. 1 hit.
PF09744. Jnk-SapK_ap_N. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51776. RH1. 1 hit.
PS51777. RH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJIP3_HUMAN
AccessioniPrimary (citable) accession number: Q9UPT6
Secondary accession number(s): A2A2B3
, A7E2B3, Q96RY4, Q9H4I4, Q9H7P1, Q9NUG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: April 17, 2007
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.