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Protein

C-Jun-amino-terminal kinase-interacting protein 3

Gene

MAPK8IP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

SignaLinkiQ9UPT6
SIGNORiQ9UPT6

Names & Taxonomyi

Protein namesi
Recommended name:
C-Jun-amino-terminal kinase-interacting protein 3
Short name:
JIP-3
Short name:
JNK-interacting protein 3
Alternative name(s):
JNK MAP kinase scaffold protein 3
Mitogen-activated protein kinase 8-interacting protein 3
Gene namesi
Name:MAPK8IP3
Synonyms:JIP3, KIAA1066
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000138834.12
HGNCiHGNC:6884 MAPK8IP3
MIMi605431 gene
neXtProtiNX_Q9UPT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi23162
OpenTargetsiENSG00000138834
PharmGKBiPA30628

Polymorphism and mutation databases

BioMutaiMAPK8IP3
DMDMi145559484

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206331 – 1336C-Jun-amino-terminal kinase-interacting protein 3Add BLAST1336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265Phosphothreonine; by MAPKBy similarity1
Modified residuei275Phosphothreonine; by MAPKBy similarity1
Modified residuei286Phosphothreonine; by MAPKBy similarity1
Modified residuei314Phosphoserine; by ROCK11 Publication1
Modified residuei364Phosphoserine; by ROCK11 Publication1
Modified residuei365Phosphoserine; by ROCK11 Publication1
Modified residuei602PhosphoserineBy similarity1
Modified residuei676PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by ROCK1 is crucial for the recruitment of JNK.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UPT6
PaxDbiQ9UPT6
PeptideAtlasiQ9UPT6
PRIDEiQ9UPT6
ProteomicsDBi85440
85441 [Q9UPT6-2]

PTM databases

iPTMnetiQ9UPT6
PhosphoSitePlusiQ9UPT6

Expressioni

Gene expression databases

BgeeiENSG00000138834
CleanExiHS_MAPK8IP3
ExpressionAtlasiQ9UPT6 baseline and differential
GenevisibleiQ9UPT6 HS

Organism-specific databases

HPAiCAB005580
HPA041785
HPA069311

Interactioni

Subunit structurei

Forms homo- or heterooligomeric complexes. The central region of MAPK8IP3 interacts with the C-terminal of MAPK8IP2 but not MAPK8IP1. Binds specific components of the JNK signaling pathway namely MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3 to the N-terminal region, MAP2K4/MKK4 and MAP2K7/MKK7 to the central region and MAP3K11 to the C-terminal region. Binds the TPR motif-containing C-terminal of kinesin light chain, KLC1. Pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location (By similarity). Interacts with ROCK1 and this interaction is enhanced by ultraviolet-B (UVB) radiation.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LDLRAP1Q5SW964EBI-717887,EBI-747813

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116774, 34 interactors
ELMiQ9UPT6
IntActiQ9UPT6, 9 interactors
MINTiQ9UPT6
STRINGi9606.ENSP00000250894

Structurei

Secondary structure

11336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi433 – 484Combined sources52

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PXJX-ray2.06A/B/C433-486[»]
ProteinModelPortaliQ9UPT6
SMRiQ9UPT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 100RH1PROSITE-ProRule annotationAdd BLAST89
Domaini520 – 594RH2PROSITE-ProRule annotationAdd BLAST75

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili58 – 177Sequence analysisAdd BLAST120
Coiled coili440 – 554Sequence analysisAdd BLAST115

Sequence similaritiesi

Belongs to the JIP scaffold family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2077 Eukaryota
ENOG410XQ19 LUCA
GeneTreeiENSGT00670000097546
HOVERGENiHBG024110
InParanoidiQ9UPT6
KOiK04436
PhylomeDBiQ9UPT6
TreeFamiTF313096

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR032486 JIP_LZII
IPR019143 JNK/Rab-associated_protein-1_N
IPR034743 RH1
IPR034744 RH2
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF16471 JIP_LZII, 1 hit
PF09744 Jnk-SapK_ap_N, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS51776 RH1, 1 hit
PS51777 RH2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UPT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE
60 70 80 90 100
VVKELMPLVV NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL
110 120 130 140 150
RRQAEEKFIE FEDALEQEKK ELQIQVEHYE FQTRQLELKA KNYADQISRL
160 170 180 190 200
EERESEMKKE YNALHQRHTE MIQTYVEHIE RSKMQQVGGN SQTESSLPGR
210 220 230 240 250
RKERPTSLNV FPLADGTVRA QIGGKLVPAG DHWHLSDLGQ LQSSSSYQCP
260 270 280 290 300
QDEMSESGQS SAAATPSTTG TKSNTPTSSV PSAAVTPLNE SLQPLGDYGV
310 320 330 340 350
GSKNSKRARE KRDSRNMEVQ VTQEMRNVSI GMGSSDEWSD VQDIIDSTPE
360 370 380 390 400
LDMCPETRLD RTGSSPTQGI VNKAFGINTD SLYHELSTAG SEVIGDVDEG
410 420 430 440 450
ADLLGEFSVR DDFFGMGKEV GNLLLENSQL LETKNALNVV KNDLIAKVDQ
460 470 480 490 500
LSGEQEVLRG ELEAAKQAKV KLENRIKELE EELKRVKSEA IIARREPKEE
510 520 530 540 550
AEDVSSYLCT ESDKIPMAQR RRFTRVEMAR VLMERNQYKE RLMELQEAVR
560 570 580 590 600
WTEMIRASRE HPSVQEKKKS TIWQFFSRLF SSSSSPPPAK RPYPSVNIHY
610 620 630 640 650
KSPTTAGFSQ RRNHAMCPIS AGSRPLEFFP DDDCTSSARR EQKREQYRQV
660 670 680 690 700
REHVRNDDGR LQACGWSLPA KYKQLSPNGG QEDTRMKNVP VPVYCRPLVE
710 720 730 740 750
KDPTMKLWCA AGVNLSGWRP NEDDAGNGVK PAPGRDPLTC DREGDGEPKS
760 770 780 790 800
AHTSPEKKKA KELPEMDATS SRVWILTSTL TTSKVVIIDA NQPGTVVDQF
810 820 830 840 850
TVCNAHVLCI SSIPAASDSD YPPGEMFLDS DVNPEDPGAD GVLAGITLVG
860 870 880 890 900
CATRCNVPRS NCSSRGDTPV LDKGQGEVAT IANGKVNPSQ STEEATEATE
910 920 930 940 950
VPDPGPSEPE TATLRPGPLT EHVFTDPAPT PSSGPQPGSE NGPEPDSSST
960 970 980 990 1000
RPEPEPSGDP TGAGSSAAPT MWLGAQNGWL YVHSAVANWK KCLHSIKLKD
1010 1020 1030 1040 1050
SVLSLVHVKG RVLVALADGT LAIFHRGEDG QWDLSNYHLM DLGHPHHSIR
1060 1070 1080 1090 1100
CMAVVYDRVW CGYKNKVHVI QPKTMQIEKS FDAHPRRESQ VRQLAWIGDG
1110 1120 1130 1140 1150
VWVSIRLDST LRLYHAHTHQ HLQDVDIEPY VSKMLGTGKL GFSFVRITAL
1160 1170 1180 1190 1200
LVAGSRLWVG TGNGVVISIP LTETVVLHRG QLLGLRANKT SPTSGEGARP
1210 1220 1230 1240 1250
GGIIHVYGDD SSDRAASSFI PYCSMAQAQL CFHGHRDAVK FFVSVPGNVL
1260 1270 1280 1290 1300
ATLNGSVLDS PAEGPGPAAP ASEVEGQKLR NVLVLSGGEG YIDFRIGDGE
1310 1320 1330
DDETEEGAGD MSQVKPVLSK AERSHIIVWQ VSYTPE
Length:1,336
Mass (Da):147,457
Last modified:April 17, 2007 - v3
Checksum:i84166F5725D74B25
GO
Isoform 2 (identifier: Q9UPT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-250: CP → VL
     251-1336: Missing.

Show »
Length:250
Mass (Da):29,052
Checksum:iE7C2A9B0793A810E
GO

Sequence cautioni

The sequence AAK61290 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA83018 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB15727 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB72318 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049667753T → A. Corresponds to variant dbSNP:rs2294619Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024430249 – 250CP → VL in isoform 2. 1 Publication2
Alternative sequenceiVSP_024431251 – 1336Missing in isoform 2. 1 PublicationAdd BLAST1086

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028989 mRNA Translation: BAA83018.2 Different initiation.
AK024437 mRNA Translation: BAB15727.1 Different initiation.
AE006639 Genomic DNA Translation: AAK61290.1 Sequence problems.
AL031718 Genomic DNA Translation: CAB72318.1 Sequence problems.
AC012180 Genomic DNA No translation available.
AL031710 Genomic DNA No translation available.
AL031717 Genomic DNA No translation available.
BC137123 mRNA Translation: AAI37124.1
BC137124 mRNA Translation: AAI37125.1
BC150266 mRNA Translation: AAI50267.1
CCDSiCCDS10442.2 [Q9UPT6-1]
RefSeqiNP_001035529.1, NM_001040439.1
NP_001305781.1, NM_001318852.1
NP_055948.2, NM_015133.4 [Q9UPT6-1]
UniGeneiHs.207763

Genome annotation databases

EnsembliENST00000250894; ENSP00000250894; ENSG00000138834 [Q9UPT6-1]
GeneIDi23162
KEGGihsa:23162
UCSCiuc002cmk.4 human [Q9UPT6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiJIP3_HUMAN
AccessioniPrimary (citable) accession number: Q9UPT6
Secondary accession number(s): A2A2B3
, A7E2B3, Q96RY4, Q9H4I4, Q9H7P1, Q9NUG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: April 17, 2007
Last modified: June 20, 2018
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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