Q9UPT6 (JIP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-Jun-amino-terminal kinase-interacting protein 3
Short name=JIP-3
Short name=JNK-interacting protein 3

Alternative name(s):
JNK MAP kinase scaffold protein 3
Mitogen-activated protein kinase 8-interacting protein 3

Gene names

Name:	MAPK8IP3
Synonyms:	JIP3, KIAA1066

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interations with the JNK-signaling components and motor proteins By similarity. Ref.7 UniProtKB Q9ESN9
Subunit structure	Forms homo- or heterooligomeric complexes. The central region of MAPK8IP3 interacts with the C-terminal of MAPK8IP2 but not MAPK8IP1. Binds specific components of the JNK signaling pathway namely MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3 to the N-terminal region, MAP2K4/MKK4 and MAP2K7/MKK7 to the central region and MAP3K11 to the C-terminal region. Binds the TPR motif-containing C-terminal of kinesin light chain, KLC1. Pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location By similarity. Interacts with ROCK1 and this interaction is enhanced by ultraviolet-B (UVB) radiation. Ref.7 Ref.9 UniProtKB Q9ESN9
Subcellular location	Cytoplasm By similarity UniProtKB Q9ESN9.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Phosphorylation by ROCK1 is crucial for the recruitment of JNK. Ref.8 Ref.9
Sequence similarities	Belongs to the JIP scaffold family.
Sequence caution	The sequence AAK61290.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAA83018.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB15727.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAB72318.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	MAP-kinase scaffold activity Inferred from sequence or structural similarity. Source: UniProtKB kinesin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UPT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UPT6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-250: CP → VL
     251-1336: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1336	1336	C-Jun-amino-terminal kinase-interacting protein 3		PRO_0000220633
Regions
	Coiled coil	58 – 177	120	Potential
	Coiled coil	440 – 554	115	Potential
Amino acid modifications
	Modified residue	265	1	Phosphothreonine; by MAPK By similarity
	Modified residue	267	1	Phosphoserine By similarity
	Modified residue	275	1	Phosphothreonine; by MAPK By similarity
	Modified residue	286	1	Phosphothreonine; by MAPK By similarity
	Modified residue	314	1	Phosphoserine; by ROCK1 Ref.9
	Modified residue	364	1	Phosphoserine; by ROCK1 Ref.9
	Modified residue	365	1	Phosphoserine; by ROCK1 Ref.9
	Modified residue	585	1	Phosphoserine Ref.8
	Modified residue	676	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	249 – 250	2	CP → VL in isoform 2.		VSP_024430
	Alternative sequence	251 – 1336	1086	Missing in isoform 2.		VSP_024431
	Natural variant	753	1	T → A. Corresponds to variant rs2294619 [ dbSNP | Ensembl ].		VAR_049667

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 17, 2007. Version 3. Checksum: 84166F5725D74B25	FASTA	1,336	147,457
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RRQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGN SQTESSLPGR RKERPTSLNV FPLADGTVRA QIGGKLVPAG DHWHLSDLGQ 250 260 270 280 290 300 LQSSSSYQCP QDEMSESGQS SAAATPSTTG TKSNTPTSSV PSAAVTPLNE SLQPLGDYGV 310 320 330 340 350 360 GSKNSKRARE KRDSRNMEVQ VTQEMRNVSI GMGSSDEWSD VQDIIDSTPE LDMCPETRLD 370 380 390 400 410 420 RTGSSPTQGI VNKAFGINTD SLYHELSTAG SEVIGDVDEG ADLLGEFSVR DDFFGMGKEV 430 440 450 460 470 480 GNLLLENSQL LETKNALNVV KNDLIAKVDQ LSGEQEVLRG ELEAAKQAKV KLENRIKELE 490 500 510 520 530 540 EELKRVKSEA IIARREPKEE AEDVSSYLCT ESDKIPMAQR RRFTRVEMAR VLMERNQYKE 550 560 570 580 590 600 RLMELQEAVR WTEMIRASRE HPSVQEKKKS TIWQFFSRLF SSSSSPPPAK RPYPSVNIHY 610 620 630 640 650 660 KSPTTAGFSQ RRNHAMCPIS AGSRPLEFFP DDDCTSSARR EQKREQYRQV REHVRNDDGR 670 680 690 700 710 720 LQACGWSLPA KYKQLSPNGG QEDTRMKNVP VPVYCRPLVE KDPTMKLWCA AGVNLSGWRP 730 740 750 760 770 780 NEDDAGNGVK PAPGRDPLTC DREGDGEPKS AHTSPEKKKA KELPEMDATS SRVWILTSTL 790 800 810 820 830 840 TTSKVVIIDA NQPGTVVDQF TVCNAHVLCI SSIPAASDSD YPPGEMFLDS DVNPEDPGAD 850 860 870 880 890 900 GVLAGITLVG CATRCNVPRS NCSSRGDTPV LDKGQGEVAT IANGKVNPSQ STEEATEATE 910 920 930 940 950 960 VPDPGPSEPE TATLRPGPLT EHVFTDPAPT PSSGPQPGSE NGPEPDSSST RPEPEPSGDP 970 980 990 1000 1010 1020 TGAGSSAAPT MWLGAQNGWL YVHSAVANWK KCLHSIKLKD SVLSLVHVKG RVLVALADGT 1030 1040 1050 1060 1070 1080 LAIFHRGEDG QWDLSNYHLM DLGHPHHSIR CMAVVYDRVW CGYKNKVHVI QPKTMQIEKS 1090 1100 1110 1120 1130 1140 FDAHPRRESQ VRQLAWIGDG VWVSIRLDST LRLYHAHTHQ HLQDVDIEPY VSKMLGTGKL 1150 1160 1170 1180 1190 1200 GFSFVRITAL LVAGSRLWVG TGNGVVISIP LTETVVLHRG QLLGLRANKT SPTSGEGARP 1210 1220 1230 1240 1250 1260 GGIIHVYGDD SSDRAASSFI PYCSMAQAQL CFHGHRDAVK FFVSVPGNVL ATLNGSVLDS 1270 1280 1290 1300 1310 1320 PAEGPGPAAP ASEVEGQKLR NVLVLSGGEG YIDFRIGDGE DDETEEGAGD MSQVKPVLSK 1330 AERSHIIVWQ VSYTPE
	Isoform 2 [UniParc]. Checksum: E7C2A9B0793A810E	FASTA	250	29,052
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RRQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGN SQTESSLPGR RKERPTSLNV FPLADGTVRA QIGGKLVPAG DHWHLSDLGQ 250 LQSSSSYQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed: 10470851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	Ohara O., Nagase T., Kikuno R. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 324; 414 AND 753.
[3]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed: 11214971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Spleen.
[4]	"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16." Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R. Hum. Mol. Genet. 10:339-352(2001) [PubMed: 11157797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[7]	"Phosphorylation-dependent scaffolding role of JSAP1/JIP3 in the ASK1-JNK signaling pathway. A new mode of regulation of the MAP kinase cascade." Matsuura H., Nishitoh H., Takeda K., Matsuzawa A., Amagasa T., Ito M., Yoshioka K., Ichijo H. J. Biol. Chem. 277:40703-40709(2002) [PubMed: 12189133] [Abstract] Cited for: INTERACTION WITH MAP2K4/MEK4, MAP2K7/MKK7, MAPK10/JNK3 AND MAP3K5/ASK1, FUNCTION.
[8]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage." Ongusaha P.P., Qi H.H., Raj L., Kim Y.B., Aaronson S.A., Davis R.J., Shi Y., Liao J.K., Lee S.W. Sci. Signal. 1:RA14-RA14(2008) [PubMed: 19036714] [Abstract] Cited for: INTERACTION WITH ROCK1, PHOSPHORYLATION AT SER-314; SER-364 AND SER-365.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB028989 mRNA. Translation: BAA83018.2. Different initiation. AK024437 mRNA. Translation: BAB15727.1. Different initiation. AE006639 Genomic DNA. Translation: AAK61290.1. Sequence problems. AL031718 Genomic DNA. Translation: CAB72318.1. Sequence problems. AL031710, AC012180, AL031717 Genomic DNA. Translation: CAM26364.1. AL031717, AC012180, AL031710 Genomic DNA. Translation: CAM26394.1. BC137123 mRNA. Translation: AAI37124.1. BC137124 mRNA. Translation: AAI37125.1. BC150266 mRNA. Translation: AAI50267.1.
IPI	IPI00219817. IPI00398504.
RefSeq	NP_001035529.1. NM_001040439.1. NP_055948.2. NM_015133.3.
UniGene	Hs.207763.
3D structure databases
ProteinModelPortal	Q9UPT6.
SMR	Q9UPT6. Positions 416-475.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UPT6. 3 interactions.
MINT	MINT-1404510.
STRING	Q9UPT6.
PTM databases
PhosphoSite	Q9UPT6.
Polymorphism databases
DMDM	145559484.
Proteomic databases
PRIDE	Q9UPT6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250894; ENSP00000250894; ENSG00000138834.
GeneID	23162.
KEGG	hsa:23162.
UCSC	uc002cmi.1. human. uc002cmk.1. human.
Organism-specific databases
CTD	23162.
GeneCards	GC16P001696.
H-InvDB	HIX0012690.
HGNC	HGNC:6884. MAPK8IP3.
HPA	CAB005580.
MIM	605431. gene.
neXtProt	NX_Q9UPT6.
PharmGKB	PA30628.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11458.
GeneTree	ENSGT00600000084097.
HOVERGEN	HBG024110.
OMA	WLYVHSA.
OrthoDB	EOG4B2SWD.
PhylomeDB	Q9UPT6.
Gene expression databases
ArrayExpress	Q9UPT6.
Bgee	Q9UPT6.
CleanEx	HS_MAPK8IP3.
Genevestigator	Q9UPT6.
GermOnline	ENSG00000138834. Homo sapiens.
Family and domain databases
InterPro	IPR019143. JNK/Rab-associated_protein-1_N. IPR015943. WD40/YVTN_repeat-like_dom. IPR011046. WD40_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K04436.
Pfam	PF09744. Jnk-SapK_ap_N. 1 hit. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
ProtoNet	Search...
Other
NextBio	44539.
SOURCE	Search...

Entry information

Entry name

JIP3_HUMAN

Accession

Primary (citable) accession number: Q9UPT6
Secondary accession number(s): A2A2B3 , A7E2B3, Q96RY4, Q9H4I4, Q9H7P1, Q9NUG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	April 17, 2007
Last modified:	December 14, 2011
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.