Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein SMG5

Gene

SMG5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.1 Publication

GO - Molecular functioni

  • DNA polymerase binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • protein phosphatase 2A binding Source: HGNC
  • ribonuclease activity Source: GO_Central
  • ribonucleoprotein complex binding Source: GO_Central
  • telomerase RNA binding Source: GO_Central
  • telomeric DNA binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: BHF-UCL

GO - Biological processi

  • mRNA export from nucleus Source: HGNC
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: HGNC
  • regulation of dephosphorylation Source: HGNC
  • regulation of RNA stability Source: GO_Central
  • regulation of telomere maintenance Source: BHF-UCL
  • regulation of telomere maintenance via telomerase Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay

Enzyme and pathway databases

BioCyciZFISH:G66-32264-MONOMER.
ReactomeiR-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SMG5
Alternative name(s):
EST1-like protein B
LPTS-RP1
LPTS-interacting protein
SMG-5 homolog
Short name:
hSMG-5
Gene namesi
Name:SMG5
Synonyms:EST1B, KIAA1089
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24644. SMG5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HGNC
  • cytosol Source: Reactome
  • nucleus Source: HGNC
  • telomerase holoenzyme complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi860D → A: Abolishes stimulation of RENT1 dephosphorylation. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000198952.
PharmGKBiPA143485617.

Polymorphism and mutation databases

BioMutaiSMG5.
DMDMi84029494.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000763222 – 1016Protein SMG5Add BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei423PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9UPR3.
MaxQBiQ9UPR3.
PaxDbiQ9UPR3.
PeptideAtlasiQ9UPR3.
PRIDEiQ9UPR3.

PTM databases

iPTMnetiQ9UPR3.
PhosphoSitePlusiQ9UPR3.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiENSG00000198952.
CleanExiHS_SMG5.
GenevisibleiQ9UPR3. HS.

Interactioni

Subunit structurei

Interacts with TERT, PPP2CA and SMG1. Part of a complex that contains SMG1, SMG5, SMG7, PPP2CA, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated UPF1. Not detected in complexes that contain unphosphorylated UPF1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SMG7Q925405EBI-3400861,EBI-719830
UPF1Q92900-22EBI-3400861,EBI-373492

GO - Molecular functioni

  • DNA polymerase binding Source: BHF-UCL
  • histone deacetylase binding Source: BHF-UCL
  • protein phosphatase 2A binding Source: HGNC
  • ubiquitin protein ligase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi116957. 16 interactors.
IntActiQ9UPR3. 8 interactors.
MINTiMINT-6783753.
STRINGi9606.ENSP00000355261.

Structurei

Secondary structure

11016
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi856 – 859Combined sources4
Helixi861 – 866Combined sources6
Helixi868 – 877Combined sources10
Beta strandi878 – 884Combined sources7
Helixi886 – 895Combined sources10
Helixi900 – 915Combined sources16
Beta strandi918 – 922Combined sources5
Helixi946 – 955Combined sources10
Beta strandi977 – 980Combined sources4
Helixi992 – 996Combined sources5
Beta strandi1000 – 1002Combined sources3
Helixi1005 – 1011Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HWYX-ray2.75A/B853-1016[»]
ProteinModelPortaliQ9UPR3.
SMRiQ9UPR3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UPR3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini872 – 995PINcAdd BLAST124

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili798 – 841Sequence analysisAdd BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi460 – 463Poly-Arg4
Compositional biasi529 – 532Poly-Glu4

Sequence similaritiesi

Contains 1 PINc domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410INQY. Eukaryota.
ENOG410XPF4. LUCA.
GeneTreeiENSGT00560000077120.
HOVERGENiHBG082268.
InParanoidiQ9UPR3.
KOiK11125.
OMAiIIPFELS.
OrthoDBiEOG091G0188.
PhylomeDBiQ9UPR3.
TreeFamiTF327119.

Family and domain databases

InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR002716. PIN_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 2 hits.
PF13638. PIN_4. 1 hit.
[Graphical view]
SMARTiSM00670. PINc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UPR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQGPPTGES SEPEAKVLHT KRLYRAVVEA VHRLDLILCN KTAYQEVFKP
60 70 80 90 100
ENISLRNKLR ELCVKLMFLH PVDYGRKAEE LLWRKVYYEV IQLIKTNKKH
110 120 130 140 150
IHSRSTLECA YRTHLVAGIG FYQHLLLYIQ SHYQLELQCC IDWTHVTDPL
160 170 180 190 200
IGCKKPVSAS GKEMDWAQMA CHRCLVYLGD LSRYQNELAG VDTELLAERF
210 220 230 240 250
YYQALSVAPQ IGMPFNQLGT LAGSKYYNVE AMYCYLRCIQ SEVSFEGAYG
260 270 280 290 300
NLKRLYDKAA KMYHQLKKCE TRKLSPGKKR CKDIKRLLVN FMYLQSLLQP
310 320 330 340 350
KSSSVDSELT SLCQSVLEDF NLCLFYLPSS PNLSLASEDE EEYESGYAFL
360 370 380 390 400
PDLLIFQMVI ICLMCVHSLE RAGSKQYSAA IAFTLALFSH LVNHVNIRLQ
410 420 430 440 450
AELEEGENPV PAFQSDGTDE PESKEPVEKE EEPDPEPPPV TPQVGEGRKS
460 470 480 490 500
RKFSRLSCLR RRRHPPKVGD DSDLSEGFES DSSHDSARAS EGSDSGSDKS
510 520 530 540 550
LEGGGTAFDA ETDSEMNSQE SRSDLEDMEE EEGTRSPTLE PPRGRSEAPD
560 570 580 590 600
SLNGPLGPSE ASIASNLQAM STQMFQTKRC FRLAPTFSNL LLQPTTNPHT
610 620 630 640 650
SASHRPCVNG DVDKPSEPAS EEGSESEGSE SSGRSCRNER SIQEKLQVLM
660 670 680 690 700
AEGLLPAVKV FLDWLRTNPD LIIVCAQSSQ SLWNRLSVLL NLLPAAGELQ
710 720 730 740 750
ESGLALCPEV QDLLEGCELP DLPSSLLLPE DMALRNLPPL RAAHRRFNFD
760 770 780 790 800
TDRPLLSTLE ESVVRICCIR SFGHFIARLQ GSILQFNPEV GIFVSIAQSE
810 820 830 840 850
QESLLQQAQA QFRMAQEEAR RNRLMRDMAQ LRLQLEVSQL EGSLQQPKAQ
860 870 880 890 900
SAMSPYLVPD TQALCHHLPV IRQLATSGRF IVIIPRTVID GLDLLKKEHP
910 920 930 940 950
GARDGIRYLE AEFKKGNRYI RCQKEVGKSF ERHKLKRQDA DAWTLYKILD
960 970 980 990 1000
SCKQLTLAQG AGEEDPSGMV TIITGLPLDN PSVLSGPMQA ALQAAAHASV
1010
DIKNVLDFYK QWKEIG
Length:1,016
Mass (Da):113,928
Last modified:December 20, 2005 - v3
Checksum:iB6F8F9B53CF42195
GO

Sequence cautioni

The sequence BAA83041 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti372A → S in AAQ84301 (Ref. 3) Curated1
Sequence conflicti384T → A in AAQ84301 (Ref. 3) Curated1
Sequence conflicti496G → R in AAQ84301 (Ref. 3) Curated1
Sequence conflicti915K → KK in CAH56374 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0308281004N → D.1 PublicationCorresponds to variant rs17853821dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085691 mRNA. Translation: BAC53623.1.
AY168922 mRNA. Translation: AAO17582.1.
AY336728 mRNA. Translation: AAQ84301.1.
AB029012 mRNA. Translation: BAA83041.2. Different initiation.
AL589685, AL135927 Genomic DNA. Translation: CAI14160.1.
AL135927, AL589685 Genomic DNA. Translation: CAI15525.1.
CH471121 Genomic DNA. Translation: EAW52973.1.
CH471121 Genomic DNA. Translation: EAW52974.1.
BC007453 mRNA. Translation: AAH07453.2.
BC038296 mRNA. Translation: AAH38296.1.
AL137738 mRNA. Translation: CAH56374.1.
CCDSiCCDS1137.1.
RefSeqiNP_056142.2. NM_015327.2.
UniGeneiHs.516837.

Genome annotation databases

EnsembliENST00000361813; ENSP00000355261; ENSG00000198952.
GeneIDi23381.
KEGGihsa:23381.
UCSCiuc001foc.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB085691 mRNA. Translation: BAC53623.1.
AY168922 mRNA. Translation: AAO17582.1.
AY336728 mRNA. Translation: AAQ84301.1.
AB029012 mRNA. Translation: BAA83041.2. Different initiation.
AL589685, AL135927 Genomic DNA. Translation: CAI14160.1.
AL135927, AL589685 Genomic DNA. Translation: CAI15525.1.
CH471121 Genomic DNA. Translation: EAW52973.1.
CH471121 Genomic DNA. Translation: EAW52974.1.
BC007453 mRNA. Translation: AAH07453.2.
BC038296 mRNA. Translation: AAH38296.1.
AL137738 mRNA. Translation: CAH56374.1.
CCDSiCCDS1137.1.
RefSeqiNP_056142.2. NM_015327.2.
UniGeneiHs.516837.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HWYX-ray2.75A/B853-1016[»]
ProteinModelPortaliQ9UPR3.
SMRiQ9UPR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116957. 16 interactors.
IntActiQ9UPR3. 8 interactors.
MINTiMINT-6783753.
STRINGi9606.ENSP00000355261.

PTM databases

iPTMnetiQ9UPR3.
PhosphoSitePlusiQ9UPR3.

Polymorphism and mutation databases

BioMutaiSMG5.
DMDMi84029494.

Proteomic databases

EPDiQ9UPR3.
MaxQBiQ9UPR3.
PaxDbiQ9UPR3.
PeptideAtlasiQ9UPR3.
PRIDEiQ9UPR3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361813; ENSP00000355261; ENSG00000198952.
GeneIDi23381.
KEGGihsa:23381.
UCSCiuc001foc.5. human.

Organism-specific databases

CTDi23381.
GeneCardsiSMG5.
HGNCiHGNC:24644. SMG5.
MIMi610962. gene.
neXtProtiNX_Q9UPR3.
OpenTargetsiENSG00000198952.
PharmGKBiPA143485617.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INQY. Eukaryota.
ENOG410XPF4. LUCA.
GeneTreeiENSGT00560000077120.
HOVERGENiHBG082268.
InParanoidiQ9UPR3.
KOiK11125.
OMAiIIPFELS.
OrthoDBiEOG091G0188.
PhylomeDBiQ9UPR3.
TreeFamiTF327119.

Enzyme and pathway databases

BioCyciZFISH:G66-32264-MONOMER.
ReactomeiR-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiSMG5. human.
EvolutionaryTraceiQ9UPR3.
GeneWikiiSMG5.
GenomeRNAii23381.
PROiQ9UPR3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198952.
CleanExiHS_SMG5.
GenevisibleiQ9UPR3. HS.

Family and domain databases

InterProiIPR018834. DNA/RNA-bd_Est1-type.
IPR019458. EST1.
IPR002716. PIN_dom.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF10374. EST1. 1 hit.
PF10373. EST1_DNA_bind. 2 hits.
PF13638. PIN_4. 1 hit.
[Graphical view]
SMARTiSM00670. PINc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSMG5_HUMAN
AccessioniPrimary (citable) accession number: Q9UPR3
Secondary accession number(s): D3DVB7
, Q5QJE7, Q659C7, Q8IXC0, Q8IY09, Q96IJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.