Reviewed, UniProtKB/Swiss-Prot Q9UPR0 (PLCL2_HUMAN)

Names and origin

Protein names

Recommended name:
    Inactive phospholipase C-like protein 2
      Short name=Phospholipase C-L2
      Short name=PLC-L(2)
      Short name=PLC-L2
Alternative name(s):
    Phospholipase C epsilon 2

Gene names

Name:	PLCL2
Synonyms:	KIAA1092, PLCE2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum By similarity.
Subcellular location	Cytoplasm. Note: Predominantly localized to perinuclear areas in both myoblast and myotube C2C12 cells By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 PH domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.
Caution	The PI-PLC X-box lacks the essential His at position 486 which is replaced by a Thr and is predicted to be inactive.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Transducer
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9UPR0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9UPR0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.
     1069-1086: GQADLLKYAKNETLENLK → IRKTGRRSIFVPQTPIMV
     1087-1127: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9UPR0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-126: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1127	1127	Inactive phospholipase C-like protein 2		PRO_0000288851
Regions
	Domain	141 – 251	111	PH
	Domain	426 – 570	145	PI-PLC X-box
	Domain	618 – 734	117	PI-PLC Y-box
	Domain	741 – 846	106	C2
	Compositional bias	5 – 65	61	Gly-rich
Amino acid modifications
	Modified residue	584	1	Phosphothreonine Ref.5
	Modified residue	1113	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 126	126	Missing in isoform 2 and isoform 3.		VSP_025790
	Alternative sequence	1069 – 1086	18	GQADL…LENLK → IRKTGRRSIFVPQTPIMV in isoform 2.		VSP_025791
	Alternative sequence	1087 – 1127	41	Missing in isoform 2.		VSP_025792
	Natural variant	211	1	Q → R: dbSNP rs17853614. Ref.3		VAR_032507
	Natural variant	635	1	V → M: dbSNP rs17857109. Ref.3		VAR_032508
	Natural variant	742	1	A → V: dbSNP rs17857110. Ref.3		VAR_032509
	Natural variant	809	1	P → H: dbSNP rs17853612. Ref.3		VAR_032510
	Natural variant	890	1	H → R: dbSNP rs17853613. Ref.3		VAR_032511

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: F75F774DAE18EC5F	FASTA	1,127	125,866
	10 20 30 40 50 60 MAECGRGGAA GGALPTSPGP ALGAKGALKA GVGEGGGGGG RLGHGRARYD SGGVSNGDCS 70 80 90 100 110 120 LGVSGDEARA SPTRGPRGVA LAPTPSAVVC TLPRESKPGG LPRRSSIIKD GTKQKRERKK 130 140 150 160 170 180 TVSFSSMPTE KKISSASDCI NSMVEGSELK KVRSNSRIYH RYFLLDADMQ SLRWEPSKKD 190 200 210 220 230 240 SEKAKIDIKS IKEVRTGKNT DIFRSNGISD QISEDCAFSV IYGENYESLD LVANSADVAN 250 260 270 280 290 300 IWVTGLRYLI SYGKHTLDML ESSQDNMRTS WVSQMFSEID VDNLGHITLC NAVQCIRNLN 310 320 330 340 350 360 PGLKTSKIEL KFKELHKSKD KAGTEVTKEE FIEVFHELCT RPEIYFLLVQ FSSNKEFLDT 370 380 390 400 410 420 KDLMMFLEAE QGVAHINEEI SLEIIHKYEP SKEGQEKGWL SIDGFTNYLM SPDCYIFDPE 430 440 450 460 470 480 HKKVCQDMKQ PLSHYFINSS HNTYLIEDQF RGPSDITGYI RALKMGCRSV ELDVWDGPDN 490 500 510 520 530 540 EPVIYTGHTM TSQIVFRSVI DIINKYAFFA SEYPLILCLE NHCSIKQQKV MVQHMKKLLG 550 560 570 580 590 600 DKLYTTSPNV EESYLPSPDV LKGKILIKAK KLSSNCSGVE GDVTDEDEGA EMSQRMGKEN 610 620 630 640 650 660 MEQPNNVPVK RFQLCKELSE LVSICKSVQF KEFQVSFQVQ KYWEVCSFNE VLASKYANEN 670 680 690 700 710 720 PGDFVNYNKR FLARVFPSPM RIDSSNMNPQ DFWKCGCQIV AMNFQTPGLM MDLNIGWFRQ 730 740 750 760 770 780 NGNCGYVLRP AIMREEVSFF SANTKDSVPG VSPQLLHIKI ISGQNFPKPK GSGAKGDVVD 790 800 810 820 830 840 PYVYVEIHGI PADCAEQRTK TVHQNGDAPI FDESFEFQIN LPELAMVRFV VLDDDYIGDE 850 860 870 880 890 900 FIGQYTIPFE CLQTGYRHVP LQSLTGEVLA HASLFVHVAI TNRRGGGKPH KRGLSVRKGK 910 920 930 940 950 960 KSREYASLRT LWIKTVDEVF KNAQPPIRDA TDLRENMQNA VVSFKELCGL SSVANLMQCM 970 980 990 1000 1010 1020 LAVSPRFLGP DNTPLVVLNL SEQYPTMELQ GIVPEVLKKI VTTYDMMIQS LKALIENADA 1030 1040 1050 1060 1070 1080 VYEKIVHCQK AAMEFHEHLH SIGTKEGLKE RKLQKAVESF TWNITILKGQ ADLLKYAKNE 1090 1100 1110 1120 TLENLKQIHF AAVSCGLNKP GTENADVQKP RRSLEVIPEK ANDETGE
	Isoform 2. Checksum: DD532227D68B5986	FASTA	960	109,221
	10 20 30 40 50 60 MPTEKKISSA SDCINSMVEG SELKKVRSNS RIYHRYFLLD ADMQSLRWEP SKKDSEKAKI 70 80 90 100 110 120 DIKSIKEVRT GKNTDIFRSN GISDQISEDC AFSVIYGENY ESLDLVANSA DVANIWVTGL 130 140 150 160 170 180 RYLISYGKHT LDMLESSQDN MRTSWVSQMF SEIDVDNLGH ITLCNAVQCI RNLNPGLKTS 190 200 210 220 230 240 KIELKFKELH KSKDKAGTEV TKEEFIEVFH ELCTRPEIYF LLVQFSSNKE FLDTKDLMMF 250 260 270 280 290 300 LEAEQGVAHI NEEISLEIIH KYEPSKEGQE KGWLSIDGFT NYLMSPDCYI FDPEHKKVCQ 310 320 330 340 350 360 DMKQPLSHYF INSSHNTYLI EDQFRGPSDI TGYIRALKMG CRSVELDVWD GPDNEPVIYT 370 380 390 400 410 420 GHTMTSQIVF RSVIDIINKY AFFASEYPLI LCLENHCSIK QQKVMVQHMK KLLGDKLYTT 430 440 450 460 470 480 SPNVEESYLP SPDVLKGKIL IKAKKLSSNC SGVEGDVTDE DEGAEMSQRM GKENMEQPNN 490 500 510 520 530 540 VPVKRFQLCK ELSELVSICK SVQFKEFQVS FQVQKYWEVC SFNEVLASKY ANENPGDFVN 550 560 570 580 590 600 YNKRFLARVF PSPMRIDSSN MNPQDFWKCG CQIVAMNFQT PGLMMDLNIG WFRQNGNCGY 610 620 630 640 650 660 VLRPAIMREE VSFFSANTKD SVPGVSPQLL HIKIISGQNF PKPKGSGAKG DVVDPYVYVE 670 680 690 700 710 720 IHGIPADCAE QRTKTVHQNG DAPIFDESFE FQINLPELAM VRFVVLDDDY IGDEFIGQYT 730 740 750 760 770 780 IPFECLQTGY RHVPLQSLTG EVLAHASLFV HVAITNRRGG GKPHKRGLSV RKGKKSREYA 790 800 810 820 830 840 SLRTLWIKTV DEVFKNAQPP IRDATDLREN MQNAVVSFKE LCGLSSVANL MQCMLAVSPR 850 860 870 880 890 900 FLGPDNTPLV VLNLSEQYPT MELQGIVPEV LKKIVTTYDM MIQSLKALIE NADAVYEKIV 910 920 930 940 950 960 HCQKAAMEFH EHLHSIGTKE GLKERKLQKA VESFTWNITI LKIRKTGRRS IFVPQTPIMV
	Isoform 3. Checksum: 7208D87B3EA88134	FASTA	1,001	113,602
	10 20 30 40 50 60 MPTEKKISSA SDCINSMVEG SELKKVRSNS RIYHRYFLLD ADMQSLRWEP SKKDSEKAKI 70 80 90 100 110 120 DIKSIKEVRT GKNTDIFRSN GISDQISEDC AFSVIYGENY ESLDLVANSA DVANIWVTGL 130 140 150 160 170 180 RYLISYGKHT LDMLESSQDN MRTSWVSQMF SEIDVDNLGH ITLCNAVQCI RNLNPGLKTS 190 200 210 220 230 240 KIELKFKELH KSKDKAGTEV TKEEFIEVFH ELCTRPEIYF LLVQFSSNKE FLDTKDLMMF 250 260 270 280 290 300 LEAEQGVAHI NEEISLEIIH KYEPSKEGQE KGWLSIDGFT NYLMSPDCYI FDPEHKKVCQ 310 320 330 340 350 360 DMKQPLSHYF INSSHNTYLI EDQFRGPSDI TGYIRALKMG CRSVELDVWD GPDNEPVIYT 370 380 390 400 410 420 GHTMTSQIVF RSVIDIINKY AFFASEYPLI LCLENHCSIK QQKVMVQHMK KLLGDKLYTT 430 440 450 460 470 480 SPNVEESYLP SPDVLKGKIL IKAKKLSSNC SGVEGDVTDE DEGAEMSQRM GKENMEQPNN 490 500 510 520 530 540 VPVKRFQLCK ELSELVSICK SVQFKEFQVS FQVQKYWEVC SFNEVLASKY ANENPGDFVN 550 560 570 580 590 600 YNKRFLARVF PSPMRIDSSN MNPQDFWKCG CQIVAMNFQT PGLMMDLNIG WFRQNGNCGY 610 620 630 640 650 660 VLRPAIMREE VSFFSANTKD SVPGVSPQLL HIKIISGQNF PKPKGSGAKG DVVDPYVYVE 670 680 690 700 710 720 IHGIPADCAE QRTKTVHQNG DAPIFDESFE FQINLPELAM VRFVVLDDDY IGDEFIGQYT 730 740 750 760 770 780 IPFECLQTGY RHVPLQSLTG EVLAHASLFV HVAITNRRGG GKPHKRGLSV RKGKKSREYA 790 800 810 820 830 840 SLRTLWIKTV DEVFKNAQPP IRDATDLREN MQNAVVSFKE LCGLSSVANL MQCMLAVSPR 850 860 870 880 890 900 FLGPDNTPLV VLNLSEQYPT MELQGIVPEV LKKIVTTYDM MIQSLKALIE NADAVYEKIV 910 920 930 940 950 960 HCQKAAMEFH EHLHSIGTKE GLKERKLQKA VESFTWNITI LKGQADLLKY AKNETLENLK 970 980 990 1000 QIHFAAVSCG LNKPGTENAD VQKPRRSLEV IPEKANDETG E

References

[1]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed: 10470851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 384-1127 (ISOFORM 2). Tissue: Brain and Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS ARG-211; MET-635; VAL-742; HIS-809 AND ARG-890. Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 918-1127. Tissue: Testis.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-584 AND SER-1113, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB029015 mRNA. Translation: BAA83044.1. Different initiation. AK023546 mRNA. Translation: BAB14606.1. Different initiation. AK291419 mRNA. Translation: BAF84108.1. BC036392 mRNA. Translation: AAH36392.1. AL117515 mRNA. Translation: CAB55974.1.
IPI	IPI00010334. IPI00789859. IPI00847504.
PIR	T17284.
RefSeq	NP_001137854.1. NP_055999.2.
UniGene	Hs.202010
3D structure databases
HSSP	HSSP built from PDB template 1QAS based on UniProtKB P10688.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9UPR0. 1 interaction.
PTM databases
PhosphoSite	Q9UPR0.
Genome annotation databases
Ensembl	ENSG00000154822. Homo sapiens. [Contig view]
GeneID	23228.
KEGG	hsa:23228.
Organism-specific databases
GeneCards	GC03P016901.
HGNC	HGNC:9064. PLCL2.
PharmGKB	PA33395.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9UPR0.
HOVERGEN	Q9UPR0.
OMA	Q9UPR0. SSMPTEK.
Gene expression databases
ArrayExpress	Q9UPR0.
Bgee	Q9UPR0.
CleanEx	HS_PLCL2.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR011992. EF-Hand_type. IPR011993. PH_type. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR000909. Phospholipase_C_Pinositol-sp_X. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 2 hits. G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00169. PH. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
ProDom	PD001202. PI_PLC_Y. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00233. PH. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	44837.

Entry information

Entry name

PLCL2_HUMAN

Accession

Primary (citable) accession number: Q9UPR0
Secondary accession number(s): A8K5V4 , Q8N498, Q9H8L0, Q9UFP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)